FastQCFastQC Report
Mon 15 Aug 2016
SRR2920855_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920855_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2572733
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT25770.10016585475445762No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43100.043.2591631
GGTATCA23550.041.605011
TCAACGC55300.033.1355134
ATCAACG56600.032.7099383
TATCAAC57200.031.7834072
CAACGCA57700.031.5890245
AACGCAG59450.030.978526
ACGCAGA70100.025.9335257
CGCAGAG74200.024.433248
GTACATG84600.024.2312761
TACATGG85500.023.2064062
ACATGGG88750.021.9839123
TAAGGCG6250.021.2646795
GCAGAGT84300.020.6622229
CGCTAAC1200.00478388919.7781872
AGAGTAC71300.019.43503610-11
CATGGGG63350.018.3590834
GTATTAA11500.017.784431
GTATAGG4052.7284841E-1117.6159291
GAGTACA65800.017.5650621
Produced by FastQC (version 0.11.5)