##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920855_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2572733
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.263636762928762	31.0	30.0	34.0	27.0	34.0
2	30.28447569180323	31.0	30.0	34.0	26.0	34.0
3	30.423717501971637	31.0	30.0	34.0	27.0	34.0
4	34.06393473399688	35.0	35.0	37.0	30.0	37.0
5	33.58763385085044	35.0	35.0	37.0	28.0	37.0
6	33.53118687403629	35.0	35.0	37.0	28.0	37.0
7	33.35056766481403	35.0	35.0	37.0	27.0	37.0
8	33.282972232252625	35.0	33.0	37.0	27.0	37.0
9	34.62702386916948	38.0	35.0	39.0	27.0	39.0
10-11	34.43076623186316	38.0	34.0	39.0	27.0	39.0
12-13	34.2496426951417	37.0	34.0	39.0	26.5	39.0
14-15	35.148478680065125	38.0	34.0	40.0	26.0	41.0
16-17	35.01587514133802	38.0	34.0	40.0	25.5	41.0
18-19	34.92629724887891	38.5	34.0	40.0	25.0	41.0
20-21	34.80915061920533	38.0	34.0	40.0	25.0	41.0
22-23	34.66061227496208	38.0	34.0	40.0	24.5	41.0
24-25	34.475633110781416	38.0	33.0	40.0	24.0	41.0
26-27	34.28616591772251	38.0	33.0	40.0	23.0	41.0
28-29	34.22008949238028	38.0	33.0	40.0	23.0	41.0
30-31	34.211684034060276	38.0	33.0	40.0	23.0	41.0
32-33	34.068822532303194	38.0	33.0	40.0	22.0	41.0
34-35	33.898104272771405	38.0	33.0	40.0	21.0	41.0
36-37	33.69899985735014	38.0	33.0	40.0	19.0	41.0
38-39	33.51132667089823	38.0	32.0	40.0	18.5	41.0
40-41	33.284470444465086	38.0	32.0	40.0	17.0	41.0
42-43	33.314348787845454	38.0	32.0	40.0	17.0	41.0
44-45	33.16831769950477	38.0	32.0	40.0	15.0	41.0
46-47	32.91668140456083	37.5	32.0	40.0	14.5	41.0
48-49	32.65342944642914	37.0	31.0	40.0	10.5	41.0
50-51	32.38990929101465	37.0	31.0	40.0	9.0	41.0
52-53	32.12559426104458	37.0	31.0	40.0	8.0	41.0
54-55	31.828240435365814	36.0	30.0	39.5	7.0	41.0
56-57	31.4925143806217	36.0	30.0	39.0	4.0	41.0
58-59	31.138064462966035	35.5	29.5	39.0	2.0	40.0
60-61	30.790068965570853	35.0	29.0	39.0	2.0	40.0
62-63	30.453348248730045	35.0	28.5	38.5	2.0	40.0
64-65	30.037658979769763	35.0	28.0	38.0	2.0	40.0
66-67	29.59456092023541	34.0	28.0	37.5	2.0	40.0
68-69	29.141341717154482	34.0	27.5	37.0	2.0	39.5
70-71	28.71827993810473	34.0	26.5	36.5	2.0	39.0
72-73	28.289583878311507	34.0	26.0	36.0	2.0	39.0
74-75	27.853300167564996	33.0	26.0	35.5	2.0	38.0
76-77	26.405588143036994	31.5	24.0	34.0	2.0	36.0
78-79	27.25132339811399	33.0	26.0	35.0	2.0	37.0
80-81	27.291404315955056	33.0	26.0	35.0	2.0	36.5
82-83	26.96529099599531	33.0	25.0	35.0	2.0	36.0
84-85	26.660141374950296	33.0	25.0	35.0	2.0	36.0
86-87	26.130420257368332	32.5	24.0	35.0	2.0	35.0
88-89	25.771408848100442	32.0	22.5	35.0	2.0	35.0
90-91	25.409280714322087	32.0	20.0	34.5	2.0	35.0
92-93	25.10880763763671	32.0	19.5	34.0	2.0	35.0
94-95	24.740331779473422	32.0	17.5	34.0	2.0	35.0
96-97	24.222432914725314	32.0	6.0	34.0	2.0	35.0
98-99	23.62771710084179	31.0	2.0	34.0	2.0	35.0
100-101	21.409115909035258	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	59354.0
3	27094.0
4	14669.0
5	10672.0
6	9173.0
7	9244.0
8	10249.0
9	12008.0
10	13390.0
11	15130.0
12	16427.0
13	18327.0
14	19540.0
15	20592.0
16	21959.0
17	22860.0
18	22268.0
19	21510.0
20	21566.0
21	22458.0
22	24545.0
23	27902.0
24	32188.0
25	37887.0
26	44743.0
27	52528.0
28	61908.0
29	73206.0
30	86125.0
31	102127.0
32	122649.0
33	149746.0
34	189318.0
35	245883.0
36	327588.0
37	379601.0
38	211555.0
39	14743.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.11958902930547	19.33042980094459	13.789039665035748	24.760941504714186
2	16.27965280501319	20.635565369589457	37.637718332994524	25.447063492402826
3	19.15933536436744	24.286964360754883	29.31340447524643	27.24029579963124
4	14.06660672224937	16.599200041362426	35.16653624285342	34.16765699353479
5	14.847793874127134	36.72332350137839	33.7236313654393	14.705251259055169
6	33.85179654057815	34.81332639679809	16.842589807573404	14.492287255050357
7	29.40087925331199	31.381301192137943	20.62107483151018	18.59674472303988
8	26.915591200958005	34.10043249541644	19.95382771870246	19.0301485849231
9	26.723943975567355	15.44221886772476	19.266864978190142	38.56697217851774
10-11	25.942361333785772	25.12514748413086	27.37151758446677	21.560973597616595
12-13	26.385951422092802	22.46472348292784	27.2542784974058	23.895046597573554
14-15	23.65018998090073	24.099672650688866	24.552677088505728	27.697460279904675
16-17	23.945708622583446	27.132911658270505	25.23799929677606	23.68338042236999
18-19	23.96162728864453	26.2562527205826	26.637241260195253	23.144878730577616
20-21	24.7481851581035	25.469980816792102	26.227374381289536	23.55445964381486
22-23	24.45728466007241	25.34018239926989	26.18145512113172	24.021077819525985
24-25	24.206741749575546	25.5748913999494	26.17390047978675	24.0444663706883
26-27	24.057331674600878	25.75254094695939	26.17073755576295	24.019389822676782
28-29	24.051007484929237	25.723460853341816	26.248599662246896	23.976931999482055
30-31	24.31471385540705	25.5280691812957	26.0520803667845	24.105136596512747
32-33	24.022423045929916	25.706076132209265	25.89006408667497	24.38143673518585
34-35	24.301444017356356	25.61718591757481	26.100896548050912	23.980473517017927
36-37	24.46541629966352	25.404328767687833	25.94714027195589	24.183114660692752
38-39	24.312970881139947	25.439761235534565	25.932761176823266	24.31450670650222
40-41	24.374566619616488	25.342850416774148	26.15375164774454	24.128831315864826
42-43	24.388881405585888	25.19857479036547	26.014876806367297	24.39766699768134
44-45	24.098587623009998	25.32828910202652	26.06694201155733	24.50618126340615
46-47	24.248700795124464	25.17534478431284	26.0053078285903	24.57064659197239
48-49	24.01568428972399	25.33118912439813	25.962245829511676	24.690880756366205
50-51	23.822175813952946	25.45065511401396	26.112037743088635	24.61513132894446
52-53	24.0703686650544	25.280794599787644	26.109986315217242	24.538850419940715
54-55	23.892184120379227	25.183388767860727	26.139653802435387	24.784773309324663
56-57	23.784499015875625	25.384966870954834	25.94646293436016	24.884071178809382
58-59	23.72403544682859	25.278468593764696	26.07226345523396	24.925232504172754
60-61	23.68049919570785	25.26923618974491	26.137716199075673	24.91254841547157
62-63	23.54883344765262	25.38632565116803	26.009130900668172	25.055710000511183
64-65	23.43220461693022	25.314439524775672	25.90387333052549	25.349482527768618
66-67	23.426213874433856	25.28570414778709	25.91649986535999	25.371582112419055
68-69	23.5093817717796	25.157348438575948	25.89880602705405	25.434463762590404
70-71	23.314999887254555	24.885971589702997	26.000382556957835	25.798645966084617
72-73	23.254553769123266	24.918902817927783	25.887324885693996	25.939218527254955
74-75	23.14929811208305	25.037390127294685	25.87038906156178	25.94292269906049
76-77	23.15675413204138	24.84881397201295	26.137081047713913	25.857350848231754
78-79	23.072341696860583	24.88677767032365	25.994045761972078	26.046834870843682
80-81	22.91952605144531	24.799804999758	26.054749738998627	26.22591920979806
82-83	22.802142267924214	24.80286998904531	26.083107151492722	26.311880591537758
84-85	22.753144609162298	24.691331388440698	26.03355221636318	26.52197178603382
86-87	22.711393591114742	24.694117133494018	26.10112765075099	26.493361624640254
88-89	22.614889574653432	24.5854086533854	26.009072015577956	26.79062975638321
90-91	22.443426234341576	24.549371227222053	26.01528978033047	26.9919127581059
92-93	22.43668612185381	24.389603580732267	26.064873075773804	27.10883722164012
94-95	22.174527014788342	24.306682235253053	26.25714132456849	27.261649425390118
96-97	22.23906411344546	24.175786586395695	26.27382323736565	27.311326062793196
98-99	22.150476026850146	24.165344747767765	26.16169170376526	27.522487521616824
100-101	22.189227151529003	23.966471358911672	26.219711409420754	27.624590080138567
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	52.0
1	48.0
2	59.0
3	77.0
4	68.0
5	80.5
6	135.5
7	270.0
8	418.0
9	452.0
10	390.0
11	321.5
12	307.0
13	340.5
14	370.5
15	401.0
16	452.0
17	504.5
18	588.0
19	679.0
20	760.0
21	872.5
22	1060.5
23	1268.5
24	1472.5
25	1804.5
26	2302.0
27	3008.5
28	4039.0
29	5812.0
30	8571.0
31	12536.5
32	17589.5
33	22569.5
34	27376.5
35	32657.0
36	39132.0
37	45807.5
38	52351.0
39	58333.0
40	63906.5
41	69923.0
42	76441.0
43	83762.0
44	90350.0
45	96629.0
46	102650.5
47	107360.5
48	111567.5
49	114772.5
50	115381.0
51	114678.5
52	113894.5
53	113716.0
54	112075.0
55	107070.0
56	100074.0
57	91534.5
58	83544.0
59	74545.0
60	63017.5
61	51770.0
62	41463.0
63	31615.5
64	23487.0
65	17944.0
66	13886.5
67	10490.0
68	7809.0
69	5785.5
70	4274.0
71	3224.5
72	2475.0
73	1999.0
74	1511.5
75	1065.0
76	728.5
77	447.0
78	264.5
79	141.5
80	69.5
81	34.5
82	21.5
83	10.5
84	5.0
85	4.5
86	1.5
87	0.0
88	0.0
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1965225307095606
2	0.0
3	0.012282658169347538
4	0.013604209997695058
5	0.006568889970315613
6	0.10751212815321295
7	0.14517635526111725
8	0.12539194700732645
9	0.1442434951469896
10-11	0.11524709326618814
12-13	0.11520822409476617
14-15	0.11664638343737964
16-17	0.06673836733154975
18-19	0.07975953975791503
20-21	0.03702288577944155
22-23	0.08409345237146645
24-25	0.05777902331878201
26-27	0.06582494180313309
28-29	0.04011298490748943
30-31	0.025284396010001813
32-33	0.03208650100884934
34-35	0.020756137539340462
36-37	0.025711956895643658
38-39	0.032105935594560334
40-41	0.025517611038533727
42-43	0.013196083697764207
44-45	0.026664251595482313
46-47	0.0338744828942607
48-49	0.008648390641391859
50-51	0.015333888125973432
52-53	0.006510586213182634
54-55	0.012787957397833354
56-57	0.005499987756211002
58-59	0.003537094599400715
60-61	0.02314659158179259
62-63	0.010086549984005336
64-65	0.006977016270246465
66-67	0.041220756293016025
68-69	0.0902930852132732
70-71	0.022038820196265994
72-73	0.04380555619257809
74-75	0.08557048088550193
76-77	0.2599958876416636
78-79	0.08267472761456396
80-81	0.01665543995432095
82-83	0.012399265683613495
84-85	0.013779121269093995
86-87	0.2738333126678905
88-89	0.6335674941783698
90-91	0.2682555865688356
92-93	0.3589567980820396
94-95	0.0012049443140815623
96-97	0.004411650956395397
98-99	0.003265010399446814
100-101	0.004916950184881213
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2572733.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.94466721504813
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.67033547975558	42.52077404730682
2	13.0679146419769	14.882961009640583
3	5.041791980588903	8.613094995064003
4	2.448690296050647	5.577594160852871
5	1.369692295373721	3.899833597353597
6	0.8663982895331305	2.9602057363890615
7	0.5466462727836406	2.1790013061607585
8	0.39656840339805577	1.8065964607604192
9	0.2775951946272729	1.4226809380691914
>10	1.2473455552743105	11.999233325807046
>50	0.049277736474277314	1.8966980592015306
>100	0.016852333902096766	1.6889388199337676
>500	6.172063348336902E-4	0.2725033777499714
>1k	2.743139265912179E-4	0.2798841657102901
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT	2577	0.10016585475445762	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.773834284397177E-5
2	0.0	0.0	0.0	0.0	7.773834284397177E-5
3	0.0	0.0	0.0	0.0	7.773834284397177E-5
4	0.0	0.0	0.0	0.0	7.773834284397177E-5
5	0.0	0.0	0.0	0.0	7.773834284397177E-5
6	0.0	0.0	0.0	0.0	7.773834284397177E-5
7	0.0	0.0	0.0	0.0	7.773834284397177E-5
8	0.0	0.0	0.0	0.0	7.773834284397177E-5
9	0.0	0.0	0.0	0.0	7.773834284397177E-5
10-11	0.0	0.0	0.0	0.0	7.773834284397177E-5
12-13	0.0	0.0	0.0	0.0	1.5547668568794353E-4
14-15	0.0	0.0	0.0	1.943458571099294E-5	1.5547668568794353E-4
16-17	0.0	0.0	0.0	3.886917142198588E-5	1.5547668568794353E-4
18-19	0.0	0.0	0.0	3.886917142198588E-5	1.7491127139893645E-4
20-21	0.0	0.0	0.0	7.773834284397177E-5	1.943458571099294E-4
22-23	0.0	0.0	0.0	2.915187856648941E-4	1.943458571099294E-4
24-25	0.0	0.0	0.0	0.0010689022141046117	2.1378044282092235E-4
26-27	0.0	0.0	0.0	0.0025070615567180893	2.3321502853191527E-4
28-29	0.0	0.0	0.0	0.008084787655773064	2.3321502853191527E-4
30-31	0.0	0.0	0.0	0.031250813823276644	2.3321502853191527E-4
32-33	0.0	3.886917142198588E-5	0.0	0.0856482192283459	2.3321502853191527E-4
34-35	0.0	3.886917142198588E-5	0.0	0.16122932305839743	2.7208419995390117E-4
36-37	0.0	3.886917142198588E-5	0.0	0.2853968911659313	2.7208419995390117E-4
38-39	0.0	3.886917142198588E-5	0.0	0.48557312398915864	2.7208419995390117E-4
40-41	0.0	3.886917142198588E-5	0.0	0.7144153707361005	2.7208419995390117E-4
42-43	0.0	3.886917142198588E-5	0.0	0.9530915178528048	3.1095337137588706E-4
44-45	0.0	3.886917142198588E-5	0.0	1.2508099363595058	3.1095337137588706E-4
46-47	0.0	3.886917142198588E-5	0.0	1.6013515588286853	3.1095337137588706E-4
48-49	0.0	3.886917142198588E-5	0.0	1.9615521703962284	3.1095337137588706E-4
50-51	0.0	3.886917142198588E-5	0.0	2.3358622911899527	3.1095337137588706E-4
52-53	0.0	3.886917142198588E-5	0.0	2.7148367125543147	3.498225427978729E-4
54-55	0.0	3.886917142198588E-5	0.0	3.1498799136948916	3.498225427978729E-4
56-57	0.0	3.886917142198588E-5	0.0	3.658172068380201	3.498225427978729E-4
58-59	0.0	3.886917142198588E-5	0.0	4.201116089388211	3.498225427978729E-4
60-61	0.0	3.886917142198588E-5	0.0	4.767245571149435	3.498225427978729E-4
62-63	0.0	3.886917142198588E-5	0.0	5.336115329495909	3.498225427978729E-4
64-65	0.0	3.886917142198588E-5	0.0	5.957380730919221	3.498225427978729E-4
66-67	0.0	3.886917142198588E-5	0.0	6.673953340669241	3.498225427978729E-4
68-69	0.0	3.886917142198588E-5	0.0	7.45310531640866	3.498225427978729E-4
70-71	0.0	3.886917142198588E-5	0.0	8.260476310600438	3.498225427978729E-4
72-73	0.0	3.886917142198588E-5	0.0	9.068410907777837	3.692571285088659E-4
74-75	0.0	3.886917142198588E-5	0.0	9.911094544206492	3.886917142198588E-4
76-77	0.0	3.886917142198588E-5	0.0	10.839368096106359	3.886917142198588E-4
78-79	0.0	3.886917142198588E-5	0.0	11.841395900779442	3.886917142198588E-4
80-81	0.0	3.886917142198588E-5	0.0	12.87067099461934	4.081262999308517E-4
82-83	0.0	3.886917142198588E-5	0.0	13.906728758872374	4.275608856418447E-4
84-85	0.0	3.886917142198588E-5	0.0	14.966166329735731	4.6643005706383054E-4
86-87	0.0	3.886917142198588E-5	0.0	16.070322882320085	5.052992284858165E-4
88-89	0.0	3.886917142198588E-5	0.0	17.247320262149238	5.441683999078023E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4310	0.0	43.259163	1
GGTATCA	2355	0.0	41.60501	1
TCAACGC	5530	0.0	33.135513	4
ATCAACG	5660	0.0	32.709938	3
TATCAAC	5720	0.0	31.783407	2
CAACGCA	5770	0.0	31.589024	5
AACGCAG	5945	0.0	30.97852	6
ACGCAGA	7010	0.0	25.933525	7
CGCAGAG	7420	0.0	24.43324	8
GTACATG	8460	0.0	24.231276	1
TACATGG	8550	0.0	23.206406	2
ACATGGG	8875	0.0	21.983912	3
TAAGGCG	625	0.0	21.264679	5
GCAGAGT	8430	0.0	20.662222	9
CGCTAAC	120	0.004783889	19.778187	2
AGAGTAC	7130	0.0	19.435036	10-11
CATGGGG	6335	0.0	18.359083	4
GTATTAA	1150	0.0	17.78443	1
GTATAGG	405	2.7284841E-11	17.615929	1
GAGTACA	6580	0.0	17.565062	1
>>END_MODULE