FastQCFastQC Report
Mon 15 Aug 2016
SRR2920854_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920854_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2854122
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30300.044.048471
GTATCAA50950.039.022491
TCAACGC65050.030.0255224
CAACGCA66900.029.3322855
ATCAACG66950.029.1049333
TATCAAC67900.028.361592
AACGCAG71150.027.7042056
GTACATG99450.024.2957291
ACGCAGA82200.023.420667
TACATGG102350.022.936822
CGCAGAG88650.021.4577148
ACATGGG107500.021.3250353
CATGGGG73800.019.6938744
GCAGAGT96050.018.4680089
GTATAGG7100.017.5017241
AGAGTAC81050.017.39026510-11
TAAGGCG6400.017.193315
GAGTACA76100.016.9335821
CAGAGTA87550.016.4394610-11
GCACCGT5050.016.3392936
Produced by FastQC (version 0.11.5)