##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920854_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2854122
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.520542219288455	31.0	29.0	33.0	22.0	34.0
2	28.552369520293805	31.0	28.0	34.0	16.0	34.0
3	28.619279764495	31.0	28.0	34.0	18.0	34.0
4	32.09047370785131	35.0	32.0	37.0	22.0	37.0
5	32.04926944258164	35.0	32.0	37.0	22.0	37.0
6	32.16893146123396	35.0	32.0	37.0	23.0	37.0
7	32.00538484339492	35.0	32.0	37.0	22.0	37.0
8	31.953602894340186	35.0	32.0	37.0	21.0	37.0
9	33.209431832276266	37.0	33.0	39.0	19.0	39.0
10-11	33.01714415151139	37.0	32.0	39.0	18.0	39.0
12-13	32.84091044461309	37.0	32.0	39.0	17.0	39.0
14-15	33.59084177200554	38.0	32.0	40.0	17.0	41.0
16-17	33.47503295234051	38.0	32.0	40.0	17.0	41.0
18-19	33.35697790774185	38.0	32.0	40.0	17.0	41.0
20-21	33.26047152153973	38.0	32.0	40.0	16.0	41.0
22-23	33.034623782725475	38.0	32.0	40.0	12.0	41.0
24-25	32.83053124568606	38.0	32.0	40.0	10.0	41.0
26-27	32.8646382320027	38.0	32.0	40.0	10.0	41.0
28-29	32.70733941996873	38.0	31.5	40.0	9.5	41.0
30-31	32.46073923959803	37.5	31.0	40.0	8.5	41.0
32-33	32.21169715239923	37.0	30.5	40.0	8.0	41.0
34-35	31.881952313180726	37.0	30.0	40.0	5.0	41.0
36-37	31.566773074171323	37.0	30.0	40.0	2.0	41.0
38-39	31.363162121310864	37.0	30.0	40.0	2.0	41.0
40-41	31.161017819140177	37.0	30.0	40.0	2.0	41.0
42-43	30.922676045382783	36.0	29.5	40.0	2.0	41.0
44-45	30.634162625143567	36.0	28.5	39.0	2.0	41.0
46-47	30.381346172307982	36.0	28.0	39.0	2.0	40.0
48-49	30.067828039586253	35.0	27.5	39.0	2.0	40.0
50-51	28.922840894677943	34.0	25.5	38.0	2.0	39.5
52-53	29.367900356046448	34.5	27.0	38.5	2.0	39.5
54-55	30.006455400294733	35.0	28.0	39.0	2.0	40.0
56-57	29.953968505901287	35.0	28.0	39.0	2.0	40.5
58-59	29.699063845203533	35.0	28.0	39.0	2.0	40.0
60-61	29.368707434370357	35.0	27.0	39.0	2.0	40.0
62-63	28.972008554644823	34.5	26.0	38.5	2.0	40.0
64-65	28.544341131878735	34.0	26.0	38.0	2.0	40.0
66-67	28.146463605970595	34.0	26.0	37.5	2.0	40.0
68-69	27.687260215225557	34.0	25.0	37.0	2.0	39.0
70-71	27.359811879099773	33.5	24.0	36.0	2.0	39.0
72-73	26.89458386852419	33.0	23.0	36.0	2.0	39.0
74-75	26.449934515763516	33.0	22.0	35.0	2.0	38.0
76-77	26.035068402822304	32.5	20.5	35.0	2.0	37.0
78-79	25.57684254562349	32.0	19.5	35.0	2.0	37.0
80-81	25.124981342773715	32.0	17.5	35.0	2.0	36.0
82-83	24.64876010906331	31.5	12.0	35.0	2.0	36.0
84-85	24.24950387544751	31.0	7.0	34.5	2.0	35.0
86-87	23.79016366504305	31.0	2.0	34.0	2.0	35.0
88-89	23.25272710837168	31.0	2.0	34.0	2.0	35.0
90-91	22.746797088561735	30.5	2.0	34.0	2.0	35.0
92-93	22.192634547507076	30.0	2.0	34.0	2.0	35.0
94-95	21.55055460138004	29.5	2.0	34.0	2.0	35.0
96-97	20.900282468654108	29.0	2.0	34.0	2.0	35.0
98	20.499361625046163	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	152652.0
3	25513.0
4	18784.0
5	15423.0
6	14618.0
7	14867.0
8	16796.0
9	19376.0
10	21026.0
11	22239.0
12	23595.0
13	23050.0
14	21454.0
15	22541.0
16	24137.0
17	26031.0
18	26747.0
19	26875.0
20	27739.0
21	29282.0
22	31877.0
23	35785.0
24	40447.0
25	46572.0
26	53864.0
27	61648.0
28	71609.0
29	83318.0
30	96207.0
31	113448.0
32	135058.0
33	162291.0
34	201182.0
35	253695.0
36	322314.0
37	350843.0
38	201486.0
39	19733.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.90548261352922	18.09280055163873	13.361970701228524	24.639746133603524
2	16.2793721465468	19.538393763661617	38.5876766919902	25.59455739780138
3	19.40567564479875	23.61352235795293	28.583338648395003	28.397463348853314
4	13.592078205884786	15.713086225240014	35.97359261787583	34.72124295099937
5	14.950320966055786	36.26476606819122	33.691500401098054	15.093412564654937
6	33.854731217953635	34.39028723843808	17.121076242612975	14.633905300995309
7	29.643994391824485	30.90523816348722	20.73230773759317	18.71845970709513
8	27.13882395649625	33.41367001387262	20.04570710937574	19.40179892025539
9	27.102485457874607	15.411779874861692	19.336874877808306	38.14885978945539
10-11	25.959822320139082	24.94241661708925	27.30004183423133	21.797719228540334
12-13	26.587497082998418	22.34493289669995	27.098909654591974	23.968660365709656
14-15	24.022400002803064	23.880442218732014	24.537077996193084	27.560079782271835
16-17	23.990211423579833	26.717056671498586	25.360633145978245	23.93209875894334
18-19	23.982484727763556	25.976857072055793	26.731434712218803	23.309223487961845
20-21	24.88096866156808	25.25557811464459	26.307994684003265	23.555458539784063
22-23	24.628943885106057	25.06727025174787	26.08389982274043	24.21988604040564
24-25	24.30946616387271	25.3306619409504	26.101436595072116	24.25843530010478
26-27	24.067103111091008	25.501317625962532	26.081613038562406	24.349966224384048
28-29	24.134788516714206	25.52621910347129	26.133790067675772	24.20520231213873
30-31	24.48480104132687	25.381115503532058	25.871577515206322	24.262505939934748
32-33	24.164693517844725	25.535650110556496	25.709067594970907	24.59058877662787
34-35	24.578457000715233	25.476644590866286	25.751238291351335	24.193660117067147
36-37	24.61948038473239	25.234723858273806	25.725460062989967	24.420335694003832
38-39	24.447168498741867	25.32721958825892	25.77136525536043	24.454246657638787
40-41	24.533619721630714	25.221574984200572	25.920535699664182	24.32426959450453
42-43	24.472720725680315	25.164947744181532	25.770655185164394	24.59167634497376
44-45	24.34810882940404	25.253155469973947	25.855974455015463	24.542761245606552
46-47	24.455002470303572	25.204169534611697	25.683309897119948	24.65751809796478
48-49	24.298840662166114	25.208450874205013	25.70473944146513	24.787969022163743
50-51	24.21000615187803	25.302074451269895	25.797813435722727	24.690105961129348
52-53	24.278716753233446	25.13169851030968	25.966091264259934	24.62349347219694
54-55	24.101748919577865	25.171133585065093	26.047688204694886	24.67942929066216
56-57	24.031237345860372	25.153099141229923	26.000131281886674	24.815532231023035
58-59	24.00383721873431	24.84646638219494	26.08494069360966	25.06475570546109
60-61	23.92908152383178	24.918538113744656	26.247164056390137	24.905216306033424
62-63	23.851329394218077	24.952922218161863	26.276730265731054	24.919018121889007
64-65	23.81765983563199	24.902174680312893	26.16045059937977	25.119714884675346
66-67	23.82298146980539	24.946751858997057	26.058320557946494	25.171946113251053
68-69	23.99030633549462	24.769259005150023	26.0792621778332	25.161172481522154
70-71	23.80086190551402	24.71852287785404	26.198293847798503	25.282321368833436
72-73	23.75872587113628	24.80740433965632	26.061028120628272	25.372841668579127
74-75	23.85496297291338	24.923823613290857	26.0362889712768	25.18492444251896
76-77	23.870197303390224	24.72959529754803	26.147472759093226	25.252734639968523
78-79	23.810476586489816	24.810575924791237	26.129120451646422	25.249827037072524
80-81	23.891254857425825	24.875049545831345	26.119120312225785	25.11457528451705
82-83	23.890627684355252	24.741655672075076	26.199447958083216	25.168268685486456
84-85	23.830969708404687	24.778523910062976	26.11602303305617	25.274483348476174
86-87	23.867002240774717	24.780084971865048	26.193698227686973	25.159214559673266
88-89	23.832136869041864	24.58581086928531	26.17387887320574	25.40817338846708
90-91	23.873698967685616	24.71591218838621	26.150674779994393	25.25971406393378
92-93	24.077947042179026	24.56028494000097	26.160628314083894	25.201139703736107
94-95	23.944846117255636	24.52163713690082	26.183604187713822	25.349912558129724
96-97	24.10739375753298	24.37159092627626	26.169992167637186	25.35102314855358
98	24.09869775263947	24.230086559762036	26.154039309027105	25.51717637857139
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	118.0
1	89.5
2	96.0
3	142.5
4	148.5
5	163.5
6	260.0
7	494.5
8	767.5
9	912.5
10	917.5
11	942.0
12	1076.5
13	1260.5
14	1362.5
15	1440.0
16	1579.0
17	1639.5
18	1627.0
19	1684.0
20	1714.5
21	1706.0
22	1743.0
23	1897.0
24	2210.5
25	2738.5
26	3487.0
27	4374.0
28	5568.5
29	7628.0
30	10823.5
31	15638.0
32	21606.5
33	27775.0
34	34695.5
35	42456.0
36	51239.0
37	59470.5
38	65805.0
39	71997.0
40	78329.5
41	83738.5
42	90255.5
43	98479.5
44	105177.0
45	111087.5
46	116688.5
47	121275.5
48	124921.5
49	126574.5
50	126891.0
51	126543.0
52	126626.5
53	126664.0
54	124058.0
55	119655.5
56	112614.0
57	103047.5
58	94103.5
59	83310.5
60	70871.0
61	58827.0
62	47709.5
63	37108.5
64	27495.5
65	20743.5
66	16064.0
67	12553.0
68	9514.5
69	7148.0
70	5358.5
71	4016.0
72	3124.0
73	2486.0
74	2041.5
75	1572.5
76	1122.5
77	743.0
78	434.5
79	275.0
80	178.0
81	120.5
82	80.0
83	44.0
84	28.0
85	19.0
86	12.5
87	9.0
88	7.5
89	5.5
90	5.0
91	4.0
92	2.0
93	3.5
94	5.0
95	3.0
96	1.5
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4093027558037113
2	0.0273639318851822
3	0.020882078621726752
4	0.01842948549501388
5	0.02470111649046537
6	0.0223886715424218
7	0.015100966251617836
8	0.010476076355530703
9	0.0
10-11	0.0
12-13	0.005746075325441589
14-15	0.0036613711677356466
16-17	0.006411779174120798
18-19	0.03745460074937231
20-21	0.08240712905755254
22-23	0.10286876314327137
24-25	0.06749886655160502
26-27	0.07751946132646047
28-29	0.05949290184512084
30-31	0.05632204930272778
32-33	0.021144856456731705
34-35	0.36032096735878844
36-37	0.5512553422733857
38-39	0.5045509617318391
40-41	0.5122591115586509
42-43	0.46061450771901136
44-45	0.4341089834281786
46-47	0.43326809435616276
48-49	0.42133798064693795
50-51	0.41695835006352217
52-53	0.4811637344163984
54-55	0.4834061052751074
56-57	0.4522406540435202
58-59	0.43727983597057174
60-61	0.45253846892319244
62-63	0.430342501126441
64-65	0.4809710306707282
66-67	0.5028341465431401
68-69	0.44421717081470236
70-71	0.39770549401882616
72-73	0.38276219446821125
74-75	0.3641400052275271
76-77	0.36189763436881817
78-79	0.36103922677446865
80-81	0.37822489718379243
82-83	0.3741430814800488
84-85	0.3729167849166924
86-87	0.3587618188710924
88-89	0.4057289772476439
90-91	0.3818687498291944
92-93	0.3705517844016478
94-95	0.378557749108132
96-97	0.3780847490051231
98	0.373249636841032
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	2854122.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.342231209647316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.56791288360974	38.50708705765092
2	11.987846813162411	12.549412992007614
3	5.396444438473099	8.47385827525723
4	2.8363237641462917	5.938380569934496
5	1.682465199831136	4.403199124587339
6	1.0652029506971967	3.345305947835473
7	0.744449165319353	2.727629123448217
8	0.527152404843694	2.2073866445640187
9	0.3706919555408019	1.7462559640025663
>10	1.726997097218071	15.173842185464787
>50	0.07000984174212224	2.4531554595598637
>100	0.02362797253796605	2.054083252757771
>500	6.061243002679302E-4	0.22021298494548824
>1k	2.693885777934912E-4	0.20019041798423043
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.5037044667326766E-5
2	0.0	0.0	0.0	0.0	3.5037044667326766E-5
3	0.0	0.0	0.0	0.0	3.5037044667326766E-5
4	0.0	0.0	0.0	0.0	3.5037044667326766E-5
5	0.0	0.0	0.0	0.0	3.5037044667326766E-5
6	0.0	0.0	0.0	0.0	3.5037044667326766E-5
7	0.0	0.0	0.0	0.0	3.5037044667326766E-5
8	3.5037044667326766E-5	0.0	0.0	0.0	3.5037044667326766E-5
9	3.5037044667326766E-5	3.5037044667326766E-5	0.0	0.0	3.5037044667326766E-5
10-11	3.5037044667326766E-5	3.5037044667326766E-5	0.0	1.7518522333663383E-5	3.5037044667326766E-5
12-13	3.5037044667326766E-5	3.5037044667326766E-5	0.0	3.5037044667326766E-5	1.751852233366338E-4
14-15	3.5037044667326766E-5	3.5037044667326766E-5	0.0	3.5037044667326766E-5	2.2774079033762397E-4
16-17	3.5037044667326766E-5	3.5037044667326766E-5	0.0	5.2555567000990146E-5	2.6277783500495077E-4
18-19	3.5037044667326766E-5	3.5037044667326766E-5	0.0	7.007408933465353E-5	3.854074913405944E-4
20-21	3.5037044667326766E-5	3.5037044667326766E-5	0.0	1.4014817866930707E-4	4.379630583415846E-4
22-23	3.5037044667326766E-5	3.5037044667326766E-5	0.0	4.2044453600792117E-4	5.255556700099014E-4
24-25	3.5037044667326766E-5	3.5037044667326766E-5	0.0	9.284816836841593E-4	5.255556700099014E-4
26-27	3.5037044667326766E-5	3.5037044667326766E-5	0.0	0.002014630068371289	5.605927146772283E-4
28-29	3.5037044667326766E-5	3.5037044667326766E-5	0.0	0.007795742438480205	5.605927146772283E-4
30-31	3.5037044667326766E-5	3.5037044667326766E-5	0.0	0.027346413362848543	5.605927146772283E-4
32-33	3.5037044667326766E-5	3.5037044667326766E-5	0.0	0.06954853366464363	5.95629759344555E-4
34-35	3.5037044667326766E-5	3.5037044667326766E-5	0.0	0.12981225049244566	5.95629759344555E-4
36-37	3.5037044667326766E-5	3.5037044667326766E-5	0.0	0.23022842050900416	5.95629759344555E-4
38-39	3.5037044667326766E-5	3.5037044667326766E-5	0.0	0.3871768620962944	5.95629759344555E-4
40-41	3.5037044667326766E-5	3.5037044667326766E-5	0.0	0.5664789381813391	5.95629759344555E-4
42-43	3.5037044667326766E-5	3.5037044667326766E-5	0.0	0.7542249420312096	5.95629759344555E-4
44-45	3.5037044667326766E-5	3.5037044667326766E-5	0.0	0.986310325907582	5.95629759344555E-4
46-47	3.5037044667326766E-5	3.5037044667326766E-5	0.0	1.2661336831431873	6.657038486792085E-4
48-49	3.5037044667326766E-5	3.5037044667326766E-5	0.0	1.5705355272129222	7.357779380138621E-4
50-51	3.5037044667326766E-5	3.5037044667326766E-5	0.0	1.8941376717603522	7.357779380138621E-4
52-53	3.5037044667326766E-5	3.5037044667326766E-5	0.0	2.2131499634563623	8.058520273485156E-4
54-55	3.5037044667326766E-5	3.5037044667326766E-5	0.0	2.5745921162445056	8.759261166831692E-4
56-57	3.5037044667326766E-5	3.5037044667326766E-5	0.0	3.0023068390208967	8.934446390168325E-4
58-59	3.5037044667326766E-5	3.5037044667326766E-5	0.0	3.462763680038905	9.109631613504959E-4
60-61	3.5037044667326766E-5	3.5037044667326766E-5	0.0	3.9599743809129393	9.63518728351486E-4
62-63	3.5037044667326766E-5	3.5037044667326766E-5	0.0	4.452630265980221	9.810372506851494E-4
64-65	3.5037044667326766E-5	3.5037044667326766E-5	0.0	4.985280937535256	9.98555773018813E-4
66-67	3.5037044667326766E-5	3.5037044667326766E-5	0.0	5.594312366465063	0.0010335928176861396
68-69	3.5037044667326766E-5	3.5037044667326766E-5	0.0	6.2586848074469135	0.0010511113400198029
70-71	3.5037044667326766E-5	3.5037044667326766E-5	0.0	6.9634199238855246	0.0010861483846871297
72-73	3.5037044667326766E-5	3.5037044667326766E-5	0.0	7.689632748705206	0.0011036669070207932
74-75	3.5037044667326766E-5	3.5037044667326766E-5	0.0	8.431980132594191	0.0011211854293544565
76-77	3.5037044667326766E-5	3.5037044667326766E-5	0.0	9.22909041729821	0.0011211854293544565
78-79	3.5037044667326766E-5	3.5037044667326766E-5	0.0	10.108572794015112	0.0011211854293544565
80-81	3.5037044667326766E-5	3.5037044667326766E-5	0.0	11.026298104986402	0.0011211854293544565
82-83	3.5037044667326766E-5	3.5037044667326766E-5	0.0	11.952852751213857	0.0012613336080237636
84-85	3.5037044667326766E-5	3.5037044667326766E-5	0.0	12.903337698949098	0.0012613336080237636
86	3.5037044667326766E-5	7.007408933465353E-5	0.0	13.622788374147987	0.0012613336080237636
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3030	0.0	44.04847	1
GTATCAA	5095	0.0	39.02249	1
TCAACGC	6505	0.0	30.025522	4
CAACGCA	6690	0.0	29.332285	5
ATCAACG	6695	0.0	29.104933	3
TATCAAC	6790	0.0	28.36159	2
AACGCAG	7115	0.0	27.704205	6
GTACATG	9945	0.0	24.295729	1
ACGCAGA	8220	0.0	23.42066	7
TACATGG	10235	0.0	22.93682	2
CGCAGAG	8865	0.0	21.457714	8
ACATGGG	10750	0.0	21.325035	3
CATGGGG	7380	0.0	19.693874	4
GCAGAGT	9605	0.0	18.468008	9
GTATAGG	710	0.0	17.501724	1
AGAGTAC	8105	0.0	17.390265	10-11
TAAGGCG	640	0.0	17.19331	5
GAGTACA	7610	0.0	16.933582	1
CAGAGTA	8755	0.0	16.43946	10-11
GCACCGT	505	0.0	16.339293	6
>>END_MODULE