FastQCFastQC Report
Mon 15 Aug 2016
SRR2920854_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920854_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2854122
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32650.044.7154581
GTATCAA53650.040.5087621
TCAACGC69950.030.3350184
ATCAACG70900.029.9285563
TATCAAC72700.029.3155632
CAACGCA72000.029.2699245
AACGCAG76350.027.726626
GTACATG100950.024.1193791
ACGCAGA87450.023.8269357
TACATGG105500.022.810872
CGCAGAG93400.021.8505428
ACATGGG111250.020.9937253
GCAGAGT101350.019.3869539
CATGGGG73150.019.079084
AGTATCG3003.758032E-918.9858955
AGAGTAC88350.017.99485810-11
TAATACT16150.017.6361264
GAGTACA79450.017.2984221
GTATTAT8350.017.0858781
CAGAGTA94750.016.87954910-11
Produced by FastQC (version 0.11.5)