##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920854_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2854122
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.257861086526784	31.0	30.0	34.0	27.0	34.0
2	30.273386001018878	31.0	30.0	34.0	26.0	34.0
3	30.40884587274125	31.0	30.0	34.0	27.0	34.0
4	34.04963312710529	35.0	35.0	37.0	30.0	37.0
5	33.57475889257712	35.0	35.0	37.0	28.0	37.0
6	33.52205616998853	35.0	35.0	37.0	28.0	37.0
7	33.33922271017146	35.0	35.0	37.0	27.0	37.0
8	33.270770135264016	35.0	33.0	37.0	27.0	37.0
9	34.60396857597538	38.0	34.0	39.0	27.0	39.0
10-11	34.412237108294605	37.5	34.0	39.0	27.0	39.0
12-13	34.23234728578526	37.0	34.0	39.0	26.5	39.0
14-15	35.1214890253465	38.0	34.0	40.0	26.0	41.0
16-17	34.988653253084486	38.0	34.0	40.0	25.5	41.0
18-19	34.89704399461551	38.0	34.0	40.0	25.0	41.0
20-21	34.7853851377061	38.0	34.0	40.0	25.0	41.0
22-23	34.631215834501816	38.0	34.0	40.0	24.5	41.0
24-25	34.446675019498116	38.0	33.0	40.0	24.0	41.0
26-27	34.25501888146337	38.0	33.0	40.0	23.0	41.0
28-29	34.19329254320593	38.0	33.0	40.0	22.5	41.0
30-31	34.17619288874127	38.0	33.0	40.0	23.0	41.0
32-33	34.01984165358033	38.0	33.0	40.0	22.0	41.0
34-35	33.84670609735674	38.0	33.0	40.0	20.5	41.0
36-37	33.647593725846335	38.0	32.5	40.0	19.0	41.0
38-39	33.45239499222528	38.0	32.0	40.0	18.0	41.0
40-41	33.22460392372856	38.0	32.0	40.0	16.5	41.0
42-43	33.25257504759782	38.0	32.0	40.0	16.0	41.0
44-45	33.10122517537793	38.0	32.0	40.0	15.0	41.0
46-47	32.83768213131744	37.5	31.5	40.0	13.5	41.0
48-49	32.56753022470659	37.0	31.0	40.0	9.0	41.0
50-51	32.29332050276758	37.0	31.0	40.0	8.5	41.0
52-53	32.02433410344757	37.0	31.0	40.0	7.5	41.0
54-55	31.731676851935553	36.0	30.0	39.5	6.5	41.0
56-57	31.389477744819597	36.0	30.0	39.0	2.0	41.0
58-59	31.040685366638144	35.0	29.0	39.0	2.0	40.0
60-61	30.687870210173216	35.0	29.0	39.0	2.0	40.0
62-63	30.34920388827107	35.0	28.5	38.5	2.0	40.0
64-65	29.92955539391799	34.5	28.0	38.0	2.0	40.0
66-67	29.481476615225276	34.0	28.0	37.5	2.0	40.0
68-69	29.027297887055983	34.0	27.0	37.0	2.0	39.0
70-71	28.597254777476223	34.0	26.0	36.0	2.0	39.0
72-73	28.149077895058447	33.5	26.0	36.0	2.0	39.0
74-75	27.713144007158768	33.0	26.0	35.0	2.0	38.0
76-77	26.282106195880907	31.5	24.0	34.0	2.0	36.0
78-79	27.118139483876302	33.0	25.5	35.0	2.0	37.0
80-81	27.150767731722752	33.0	26.0	35.0	2.0	36.0
82-83	26.82694608009048	33.0	25.0	35.0	2.0	36.0
84-85	26.512690767948953	33.0	24.5	35.0	2.0	35.5
86-87	25.982584661762882	32.0	23.0	35.0	2.0	35.0
88-89	25.61815472499073	32.0	21.5	35.0	2.0	35.0
90-91	25.25016011929413	32.0	20.0	34.5	2.0	35.0
92-93	24.93609488311992	32.0	18.5	34.0	2.0	35.0
94-95	24.54524175911191	32.0	15.0	34.0	2.0	35.0
96-97	24.0013639921489	31.0	4.5	34.0	2.0	35.0
98-99	23.388598665368896	31.0	2.0	34.0	2.0	35.0
100-101	21.19840532394901	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	66551.0
3	30065.0
4	16665.0
5	11923.0
6	10302.0
7	10422.0
8	11734.0
9	13683.0
10	15251.0
11	16957.0
12	18621.0
13	20593.0
14	22234.0
15	23468.0
16	24805.0
17	25790.0
18	25159.0
19	24120.0
20	24206.0
21	25325.0
22	27732.0
23	31225.0
24	35637.0
25	42620.0
26	50144.0
27	59342.0
28	70063.0
29	82220.0
30	96663.0
31	114452.0
32	136461.0
33	166350.0
34	209825.0
35	273001.0
36	363435.0
37	418146.0
38	224430.0
39	14501.0
40	1.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.026679192504716	19.409045845690663	14.015071728677992	24.54920323312663
2	16.39032949537546	20.570424109410883	37.57309603443721	25.466150360776453
3	19.182857929791354	24.474314682675537	28.9911768756279	27.351650511905206
4	14.062820855155863	16.73061868408124	35.466457254471564	33.74010320629134
5	14.875738550237536	36.61691957187037	33.71552351832684	14.791818359565253
6	33.682393305568475	34.93310969925144	16.872062084412747	14.512434910767336
7	29.494890875643883	31.053995706111053	20.741574760437977	18.709538657807084
8	27.093127924345357	33.74577901508364	19.98194790078572	19.179145159785282
9	26.83092075924771	15.427960129635872	19.285213307290142	38.455905803826276
10-11	25.96914376932592	25.11542280128442	27.3082499526909	21.607183476698758
12-13	26.63432345399134	22.4019307712369	27.137571309084574	23.826174465687192
14-15	23.94801374598412	23.992421258219863	24.432024060032223	27.627540935763793
16-17	24.004282249839513	26.891084512903724	25.258191897663558	23.84644133959321
18-19	24.055751887654676	26.07988644705406	26.64042788534112	23.223933779950144
20-21	24.880102038133913	25.273838427704238	26.228553222497602	23.61750631166425
22-23	24.576843246586886	25.179531938783633	26.08715929784155	24.156465516787932
24-25	24.287567593718826	25.496590010932792	26.097691843984872	24.118150551363513
26-27	24.087812478416065	25.641575366983883	26.099391460106574	24.17122069449348
28-29	24.13330973227993	25.588560031966573	26.140982411373372	24.137147824380122
30-31	24.467694196928836	25.537553906963645	25.812382826662052	24.18236906944547
32-33	24.286857613706996	25.538154622556185	25.69715156616283	24.47783619757399
34-35	24.55744071524393	25.49682567050614	25.825910911310817	24.11982270293911
36-37	24.558008726278715	25.352380451733865	25.77691916802467	24.312691653962748
38-39	24.431875433610138	25.352570275290194	25.842068914637135	24.373485376462533
40-41	24.541211297848662	25.192221729050203	25.97630099139258	24.290265981708554
42-43	24.45804062559954	25.152041573477636	25.821898787294227	24.568019013628593
44-45	24.255321982504306	25.219107986263772	25.954266426549605	24.571303604682317
46-47	24.43821041869731	25.178442561385467	25.735404160791596	24.64794285912562
48-49	24.27314114418216	25.143502381413324	25.798384475830648	24.78497199857387
50-51	24.162521735047235	25.298263368849973	25.84891960783352	24.690295288269276
52-53	24.3136737554955	25.05428485031327	25.97881795855325	24.653223435637983
54-55	24.08258070213185	25.13480432523199	26.00511044002907	24.77750453260709
56-57	24.08339961527276	25.221375837867953	25.770961152359696	24.92426339449959
58-59	23.891132617345207	25.03965918764319	26.0023065836091	25.066901611402503
60-61	23.906245044356133	25.22709005293088	25.937408498553587	24.929256404159403
62-63	23.70551569391234	25.277438613624252	25.984033033508137	25.033012658955272
64-65	23.65459603586027	25.16956996373923	25.903089813082786	25.27274418731772
66-67	23.65593093886487	25.202890831789755	25.77526318805742	25.365915041287955
68-69	23.81892884045387	25.011888212924553	25.78460451878338	25.384578427838196
70-71	23.621918102020796	24.880382775119617	25.89204328613828	25.605655836721304
72-73	23.507852127653795	24.94775793331909	25.783508314826825	25.760881624200287
74-75	23.508709793367053	25.067670754907205	25.681888714527428	25.741730737198314
76-77	23.565659074390304	24.8146662305941	25.912546840622618	25.70712785439298
78-79	23.43667324360782	24.877651011629858	25.86310518348754	25.822570561274777
80-81	23.41122034075303	25.025537729081755	25.832056884919197	25.731185045246026
82-83	23.343365076673017	24.88301020280334	25.91858606908149	25.855038651442154
84-85	23.212764518490406	24.873945368909318	25.77021315663784	26.143076955962442
86-87	23.20289022306441	24.909728837813354	25.852694678731282	26.034686260390956
88-89	23.137202359177866	24.72022445862051	25.79021087510126	26.352362307100364
90-91	22.933168497270913	24.88253410502748	25.803711938090217	26.38058545961139
92-93	22.980590959418613	24.719918905434326	25.878375028611995	26.421115106535066
94-95	22.775050397124257	24.680899967292454	25.882705171523657	26.661344464059628
96-97	22.846507759036616	24.579269922814326	25.875193191935463	26.699029126213592
98-99	22.634920290101356	24.509177701620548	25.876871953657222	26.97903005462088
100-101	22.78392950214393	24.42720929926988	25.917352476140838	26.871508722445352
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	116.0
1	80.5
2	76.5
3	112.5
4	96.0
5	99.0
6	151.5
7	339.0
8	541.0
9	583.0
10	485.5
11	386.0
12	416.5
13	457.5
14	491.5
15	537.5
16	580.5
17	618.0
18	684.0
19	777.0
20	844.5
21	964.5
22	1167.0
23	1366.0
24	1680.0
25	2117.5
26	2754.5
27	3591.0
28	4440.0
29	5941.0
30	8577.5
31	12329.5
32	17271.5
33	22789.5
34	28908.0
35	35491.5
36	43894.5
37	52791.0
38	59516.5
39	65615.0
40	71763.5
41	77810.0
42	83915.5
43	90950.0
44	98423.0
45	105605.5
46	111408.0
47	115906.5
48	120691.5
49	124493.0
50	125811.0
51	126236.0
52	126419.0
53	125837.0
54	123667.0
55	119393.0
56	112283.0
57	102592.5
58	93872.5
59	83850.5
60	71479.0
61	59550.0
62	48707.0
63	37706.5
64	27718.5
65	20913.0
66	16097.5
67	12576.5
68	9588.5
69	7105.0
70	5474.5
71	4119.0
72	3099.5
73	2622.5
74	2097.5
75	1593.0
76	1132.5
77	728.5
78	486.5
79	309.0
80	189.5
81	121.5
82	69.5
83	36.5
84	23.0
85	13.5
86	6.0
87	5.0
88	2.0
89	0.5
90	0.5
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19655782058370314
2	0.0
3	0.012998743571578231
4	0.01471555876027724
5	0.007708149826811888
6	0.10637246761000406
7	0.14102410478599023
8	0.12206906362096645
9	0.13962262299929715
10-11	0.11178569101110605
12-13	0.11353754324447238
14-15	0.11397550630281397
16-17	0.0659747551085763
18-19	0.07836035039847632
20-21	0.037524674838706966
22-23	0.08412394424625157
24-25	0.05933523514411788
26-27	0.06604482919791095
28-29	0.040012305010087165
30-31	0.024893820236135667
32-33	0.03232167370560894
34-35	0.02123244906840002
36-37	0.023544894016443586
38-39	0.031410710544258445
40-41	0.02492885728080299
42-43	0.013296558451250507
44-45	0.027731820854189137
46-47	0.03374067401463567
48-49	0.008741742644498028
50-51	0.01527615147495447
52-53	0.006832223710128719
54-55	0.012473187901568329
56-57	0.005325630789433668
58-59	0.004116852748410895
60-61	0.023947820030117844
62-63	0.010248335565193079
64-65	0.007410334947139611
66-67	0.041045897827773306
68-69	0.08962476025902187
70-71	0.022598893810425763
72-73	0.04542552841118915
74-75	0.0857356483009486
76-77	0.26281287204961806
78-79	0.07844794301014463
80-81	0.01755355937833071
82-83	0.01217537302189605
84-85	0.01397978082226338
86-87	0.2745678005355062
88-89	0.6294229889261916
90-91	0.2682961695400547
92-93	0.3515967432366241
94-95	0.001419000309026734
96-97	0.0033285192433960427
98-99	0.002978148796722775
100-101	0.00471248250775545
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2854122.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.24102608750105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.82320841930282	34.87407453375414
2	13.11476140201929	12.915686166563676
3	5.8301617412740026	8.61249439189271
4	3.111143047908732	6.127835039360856
5	1.9173678999759545	4.720658139102654
6	1.248796096547245	3.689520070083143
7	0.8536891605182809	2.942557115658828
8	0.5767550955931678	2.272001016656187
9	0.4283841050918138	1.8984665604867117
>10	1.983743527891256	16.31948668806024
>50	0.08194084591907398	2.6922180644719114
>100	0.02897605632492727	2.3448713846084193
>500	5.720542045117632E-4	0.206158472898784
>1k	5.005474288761076E-4	0.3839723564017208
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	3.5037044667326766E-5	0.0	0.0	0.0	0.0
6	3.5037044667326766E-5	0.0	0.0	0.0	0.0
7	3.5037044667326766E-5	0.0	0.0	0.0	0.0
8	3.5037044667326766E-5	0.0	0.0	0.0	0.0
9	3.5037044667326766E-5	0.0	0.0	0.0	0.0
10-11	3.5037044667326766E-5	0.0	0.0	1.7518522333663383E-5	0.0
12-13	3.5037044667326766E-5	0.0	0.0	3.5037044667326766E-5	1.5766670100297045E-4
14-15	3.5037044667326766E-5	0.0	0.0	3.5037044667326766E-5	1.927037456702972E-4
16-17	3.5037044667326766E-5	0.0	0.0	7.007408933465353E-5	2.1022226800396058E-4
18-19	3.5037044667326766E-5	0.0	0.0	1.0511113400198029E-4	2.4525931267128736E-4
20-21	3.5037044667326766E-5	0.0	0.0	1.751852233366338E-4	2.8029635733861413E-4
22-23	3.5037044667326766E-5	0.0	0.0	5.08037147676238E-4	3.854074913405944E-4
24-25	3.5037044667326766E-5	0.0	0.0	9.985557730188127E-4	3.854074913405944E-4
26-27	3.5037044667326766E-5	0.0	0.0	0.0021197412023732693	4.0292601367425776E-4
28-29	3.5037044667326766E-5	0.0	0.0	0.007830779483147532	4.2044453600792117E-4
30-31	3.5037044667326766E-5	0.0	0.0	0.027258820751180225	4.5548158067524794E-4
32-33	3.5037044667326766E-5	0.0	0.0	0.06961860775397828	4.905186253425747E-4
34-35	3.5037044667326766E-5	0.0	0.0	0.1295144356127734	5.255556700099014E-4
36-37	3.5037044667326766E-5	0.0	0.0	0.22984301301766358	5.255556700099014E-4
38-39	3.5037044667326766E-5	0.0	0.0	0.38644108415828055	5.95629759344555E-4
40-41	3.5037044667326766E-5	0.0	0.0	0.565655567631657	6.131482816782184E-4
42-43	3.5037044667326766E-5	0.0	0.0	0.7538044974952016	6.481853263455451E-4
44-45	3.5037044667326766E-5	3.5037044667326766E-5	0.0	0.9859774739832425	6.832223710128719E-4
46-47	3.5037044667326766E-5	3.5037044667326766E-5	0.0	1.2653453496381726	7.007408933465352E-4
48-49	3.5037044667326766E-5	3.5037044667326766E-5	0.0	1.5667340078665173	7.007408933465352E-4
50-51	3.5037044667326766E-5	3.5037044667326766E-5	0.0	1.8853258550265195	7.007408933465352E-4
52-53	3.5037044667326766E-5	5.2555567000990146E-5	0.0	2.1987847751427587	7.708149826811888E-4
54-55	3.5037044667326766E-5	7.007408933465353E-5	0.0	2.5545334081724604	8.058520273485156E-4
56-57	3.5037044667326766E-5	7.007408933465353E-5	0.0	2.975713722118396	8.23370549682179E-4
58-59	3.5037044667326766E-5	7.007408933465353E-5	0.0	3.4304069692886285	8.408890720158423E-4
60-61	3.5037044667326766E-5	7.007408933465353E-5	0.0	3.9230453358335766	8.759261166831692E-4
62-63	3.5037044667326766E-5	7.007408933465353E-5	0.0	4.409937627053083	9.109631613504959E-4
64-65	3.5037044667326766E-5	7.007408933465353E-5	0.0	4.935703519330989	9.109631613504959E-4
66-67	3.5037044667326766E-5	7.007408933465353E-5	0.0	5.536361094585304	9.109631613504959E-4
68-69	3.5037044667326766E-5	7.007408933465353E-5	0.0	6.190327533300959	9.109631613504959E-4
70-71	3.5037044667326766E-5	7.007408933465353E-5	0.0	6.885007017920047	9.109631613504959E-4
72-73	3.5037044667326766E-5	7.007408933465353E-5	0.0	7.60275839645257	9.460002060178226E-4
74-75	3.5037044667326766E-5	7.007408933465353E-5	0.0	8.341812298142827	9.810372506851494E-4
76-77	3.5037044667326766E-5	7.007408933465353E-5	0.0	9.133141470476735	9.810372506851494E-4
78-79	3.5037044667326766E-5	7.007408933465353E-5	0.0	10.005984327229179	9.98555773018813E-4
80-81	3.5037044667326766E-5	7.007408933465353E-5	0.0	10.916351858820331	0.0010160742953524763
82-83	3.5037044667326766E-5	7.007408933465353E-5	0.0	11.836302022127995	0.0010511113400198029
84-85	3.5037044667326766E-5	7.007408933465353E-5	0.0	12.780042338764776	0.0010511113400198029
86-87	3.5037044667326766E-5	7.007408933465353E-5	0.0	13.750480883438058	0.0010861483846871297
88-89	3.5037044667326766E-5	7.007408933465353E-5	0.0	14.7878226649036	0.0011211854293544565
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3265	0.0	44.715458	1
GTATCAA	5365	0.0	40.508762	1
TCAACGC	6995	0.0	30.335018	4
ATCAACG	7090	0.0	29.928556	3
TATCAAC	7270	0.0	29.315563	2
CAACGCA	7200	0.0	29.269924	5
AACGCAG	7635	0.0	27.72662	6
GTACATG	10095	0.0	24.119379	1
ACGCAGA	8745	0.0	23.826935	7
TACATGG	10550	0.0	22.81087	2
CGCAGAG	9340	0.0	21.850542	8
ACATGGG	11125	0.0	20.993725	3
GCAGAGT	10135	0.0	19.386953	9
CATGGGG	7315	0.0	19.07908	4
AGTATCG	300	3.758032E-9	18.985895	5
AGAGTAC	8835	0.0	17.994858	10-11
TAATACT	1615	0.0	17.636126	4
GAGTACA	7945	0.0	17.298422	1
GTATTAT	835	0.0	17.085878	1
CAGAGTA	9475	0.0	16.879549	10-11
>>END_MODULE