FastQCFastQC Report
Mon 15 Aug 2016
SRR2920853_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920853_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3312010
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25300.048.749861
GTATCAA48750.044.0860631
TCAACGC62300.034.002394
ATCAACG63750.033.444783
CAACGCA65000.032.307825
TATCAAC68000.031.0857012
AACGCAG69600.030.694936
GTACATG99500.030.5267071
TACATGG103850.028.2580472
ACATGGG104550.027.8487193
ACGCAGA83900.024.804557
CATGGGG71700.024.1728694
CGCAGAG85800.024.0404768
GCAGAGT94950.020.9993369
GAGTACA72750.019.3574221
GTATATA11100.018.2426851
AGTACAT75150.017.7554912
AGAGTAC83800.017.72291610-11
ATGGGGA37300.017.2097575
TAAGGCG10700.017.1408255
Produced by FastQC (version 0.11.5)