##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920853_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3312010
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.717910272010048	31.0	30.0	33.0	23.0	34.0
2	28.738638470294475	31.0	28.0	34.0	22.0	34.0
3	28.814345971177623	31.0	28.0	34.0	22.0	34.0
4	32.30808693210467	35.0	32.0	37.0	24.0	37.0
5	32.278027542187374	35.0	32.0	37.0	24.0	37.0
6	32.41040214250561	35.0	33.0	37.0	25.0	37.0
7	32.24961367870266	35.0	32.0	37.0	23.0	37.0
8	32.191378347287596	35.0	32.0	37.0	23.0	37.0
9	33.467359700000905	37.0	33.0	39.0	23.0	39.0
10-11	33.27410047071114	37.0	33.0	39.0	22.0	39.0
12-13	33.0982869918871	37.0	32.0	39.0	19.0	39.0
14-15	33.86664548114287	38.0	33.0	40.0	18.0	41.0
16-17	33.75427746293036	38.0	32.5	40.0	18.0	41.0
18-19	33.644386792310414	38.0	32.0	40.0	17.5	41.0
20-21	33.55578334606477	38.0	32.0	40.0	18.0	41.0
22-23	33.33177405865321	38.0	32.0	40.0	17.5	41.0
24-25	33.14008955286971	38.0	32.0	40.0	16.0	41.0
26-27	33.18389059815641	38.0	32.0	40.0	16.0	41.0
28-29	33.02657766733796	38.0	32.0	40.0	13.5	41.0
30-31	32.78253598268121	38.0	31.5	40.0	10.0	41.0
32-33	32.544863693044405	37.5	31.0	40.0	9.0	41.0
34-35	32.21749330466997	37.0	30.5	40.0	8.5	41.0
36-37	31.912634774653455	37.0	30.0	40.0	8.0	41.0
38-39	31.718840371858782	37.0	30.0	40.0	4.5	41.0
40-41	31.523497664560193	37.0	30.0	40.0	2.0	41.0
42-43	31.28691172430035	36.5	30.0	40.0	2.0	41.0
44-45	31.009512501471917	36.0	29.5	39.5	2.0	41.0
46-47	30.758173586432406	36.0	29.0	39.0	2.0	40.0
48-49	30.459779710810054	36.0	28.0	39.0	2.0	40.0
50-51	29.32266388084577	34.0	26.5	38.0	2.0	39.5
52-53	29.779696468307765	34.5	27.5	38.5	2.0	39.5
54-55	30.43610602021129	36.0	28.0	39.0	2.0	40.0
56-57	30.394505904269614	36.0	28.0	39.0	2.0	41.0
58-59	30.153177979535087	35.0	28.0	39.0	2.0	41.0
60-61	29.837203993949295	35.0	28.0	39.0	2.0	40.0
62-63	29.450835595303154	35.0	27.5	39.0	2.0	40.0
64-65	29.033073873569222	34.0	26.5	38.0	2.0	40.0
66-67	28.647500762376925	34.0	26.0	38.0	2.0	40.0
68-69	28.209154108834213	34.0	26.0	37.0	2.0	40.0
70-71	27.880316635517403	34.0	26.0	36.5	2.0	39.0
72-73	27.413738485089116	33.5	25.0	36.0	2.0	39.0
74-75	26.964312607751786	33.0	24.0	35.5	2.0	38.5
76-77	26.539011657573496	33.0	22.5	35.0	2.0	37.5
78-79	26.063100956820783	32.0	21.5	35.0	2.0	37.0
80-81	25.59565747084097	32.0	20.0	35.0	2.0	36.5
82-83	25.107129205527762	32.0	18.0	35.0	2.0	36.0
84-85	24.688817213716142	32.0	14.5	35.0	2.0	35.5
86-87	24.20787150401116	31.0	7.0	34.0	2.0	35.0
88-89	23.642890269051122	31.0	2.0	34.0	2.0	35.0
90-91	23.124562727769543	31.0	2.0	34.0	2.0	35.0
92-93	22.559080588524793	30.0	2.0	34.0	2.0	35.0
94-95	21.8988694176648	30.0	2.0	34.0	2.0	35.0
96-97	21.23604548295446	29.0	2.0	34.0	2.0	35.0
98	20.82857358522468	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	156572.0
3	27832.0
4	20844.0
5	16905.0
6	15539.0
7	15835.0
8	18223.0
9	21178.0
10	23299.0
11	24813.0
12	25805.0
13	25537.0
14	24221.0
15	25725.0
16	27505.0
17	29443.0
18	30553.0
19	30135.0
20	31515.0
21	33369.0
22	36561.0
23	41227.0
24	46808.0
25	53803.0
26	62092.0
27	72158.0
28	83323.0
29	96704.0
30	113277.0
31	132104.0
32	156565.0
33	189197.0
34	235479.0
35	297246.0
36	378063.0
37	415009.0
38	250348.0
39	27195.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.53720551081373	17.8884995746781	12.483000765761314	25.09129414874686
2	16.18753977120177	19.69256993162751	38.94815873870135	25.171731558469368
3	19.558517095798074	23.410945417867087	28.732265487687762	28.298271998647074
4	13.405423557244264	15.278042015989582	36.12269229445136	35.19384213231479
5	14.572086852553786	36.64152891033247	33.95532946909917	14.831054768014578
6	33.94513200572599	34.51664341239785	17.13501368075815	14.403210901118015
7	29.89636645411291	31.21608008030256	20.600632467214016	18.286920998370515
8	26.84809828059092	34.33503610423893	19.974961205268293	18.841904409901858
9	27.063475049894176	15.813478824037366	19.311294349956672	37.811751776111784
10-11	25.87494087803794	25.20078822237678	27.430507068291927	21.493763831293357
12-13	26.51791777519511	22.37986450362836	27.396723798343086	23.705493922833437
14-15	23.423721285471245	24.072006607706385	24.81787969741149	27.68639240941088
16-17	23.74848514997827	27.219697828655836	25.523624413516956	23.508192607848944
18-19	23.685971528923766	26.25658703800498	27.03305446009659	23.02438697297466
20-21	24.32073209075046	25.73593368902612	26.763321066449823	23.180013153773597
22-23	24.034545550711385	25.46774766333955	26.618782443123003	23.878924342826053
24-25	23.92019948165708	25.69086204573237	26.688345864888824	23.700592607721727
26-27	23.581929313421725	26.0022886144002	26.55650859091577	23.85927348126231
28-29	23.63391184999956	26.071029242448002	26.652597252521666	23.642461655030775
30-31	23.757762122248167	25.847136395994774	26.496427509243702	23.898673972513354
32-33	23.67232581087682	25.90481252496182	26.385755475744855	24.03710618841651
34-35	24.05907252392231	25.938374611710845	26.20791769747088	23.79463516689597
36-37	24.13919613527567	25.569600116818474	26.35288803170735	23.9383157161985
38-39	23.824063505751877	25.723595165700193	26.491685594822513	23.960655733725414
40-41	24.059575505029084	25.522227925665202	26.552959603894116	23.865236965411594
42-43	24.00983616253174	25.437186790788946	26.357321427623603	24.195655619055707
44-45	23.792779471523914	25.690750508692382	26.43810114658755	24.07836887319615
46-47	23.833146702300915	25.67316797611608	26.29462960486678	24.199055716716227
48-49	23.811147298037234	25.680788865557048	26.282976776563483	24.225087059842235
50-51	23.696659905833418	25.77488850688665	26.350802356286817	24.177649230993115
52-53	23.86010673532579	25.586656734271575	26.414149021420936	24.139087508981703
54-55	23.42308643267456	25.424745886632294	26.694652887434717	24.45751479325843
56-57	23.39063522379278	25.71838646893938	26.643445665054994	24.24753264221284
58-59	23.452299115989366	25.355940652754207	26.64822210854412	24.54353812271231
60-61	23.316896812331912	25.384325873160485	26.91921832560385	24.379558988903757
62-63	23.251765373661282	25.43564682763361	26.887742277428295	24.42484552127681
64-65	23.134178145265523	25.3450860513807	26.739370063332835	24.78136574002094
66-67	23.11306112503713	25.475658524166693	26.63622496615931	24.77505538463686
68-69	23.196593622169477	25.20637109831182	26.791564356759913	24.805470922758786
70-71	23.06856875509518	25.210744646492632	26.792505961139717	24.928180637272472
72-73	23.17851812003243	25.21903910411347	26.76224608087528	24.84019669497882
74-75	23.095252200027968	25.386208446125657	26.65970431151393	24.85883504233244
76-77	23.19429039193635	25.275639307597757	26.669043296426885	24.86102700403901
78-79	23.18004336685841	25.273891163487484	26.664133499140664	24.881931970513445
80-81	23.150389965011495	25.384956912162025	26.63392081899471	24.830732303831766
82-83	23.261413524556506	25.38915820852825	26.55015532387703	24.799272943038215
84-85	23.22481900936883	25.211666495698033	26.603937086976266	24.959577407956875
86-87	23.37816396366342	25.17257088386078	26.61698043259869	24.832284719877116
88-89	23.294594216900048	25.075246626141052	26.580906682058032	25.049252474900868
90-91	23.29752650583623	25.171115256727056	26.62638101820416	24.904977219232553
92-93	23.49169397562467	24.971696060540125	26.60006654759213	24.93654341624307
94-95	23.449610376818843	24.851541634939935	26.699412439908627	24.999435548332592
96-97	23.749110330800107	24.69609566862741	26.538212854786135	25.016581145786343
98	23.718226900749258	24.67576862140903	26.443843331300886	25.162161146540825
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	54.0
1	52.0
2	101.0
3	166.0
4	173.0
5	203.5
6	322.0
7	580.5
8	905.0
9	1033.5
10	1061.0
11	1155.0
12	1310.5
13	1493.5
14	1647.0
15	1829.0
16	2024.5
17	2073.5
18	2104.5
19	2178.0
20	2234.0
21	2385.0
22	2551.0
23	2852.5
24	3347.5
25	4121.5
26	5221.0
27	6781.5
28	9351.5
29	13289.0
30	19017.5
31	26791.0
32	34706.5
33	42063.0
34	49775.0
35	58454.0
36	67520.0
37	75289.5
38	80914.5
39	86507.0
40	93579.0
41	101191.5
42	109220.0
43	116066.5
44	122967.5
45	130002.0
46	136051.5
47	142995.5
48	148448.0
49	150307.5
50	150563.0
51	150138.0
52	148742.0
53	145957.5
54	139761.0
55	131343.5
56	119800.5
57	107493.0
58	96964.0
59	83165.0
60	68949.0
61	57352.0
62	47485.5
63	36931.5
64	26977.0
65	20619.0
66	16105.0
67	12364.0
68	9183.0
69	6973.0
70	5297.5
71	4150.0
72	3384.0
73	2620.5
74	2107.0
75	1508.0
76	923.5
77	551.0
78	304.0
79	150.5
80	80.0
81	47.5
82	33.5
83	23.5
84	17.0
85	13.5
86	10.0
87	9.0
88	7.5
89	4.5
90	5.0
91	5.0
92	3.0
93	3.0
94	2.0
95	0.5
96	2.0
97	1.5
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.402535016500554
2	0.02666054752250144
3	0.020229407519904832
4	0.018236659913466447
5	0.023822391840604345
6	0.023852584986156444
7	0.01612313972481967
8	0.010537407797681772
9	0.0
10-11	1.5096572776048383E-5
12-13	0.005646118218242094
14-15	0.0036080808934755636
16-17	0.0064462365753726585
18-19	0.037635755930688616
20-21	0.08132523754457263
22-23	0.1006941404162427
24-25	0.06739110087227998
26-27	0.07801908810661803
28-29	0.06017493908532885
30-31	0.05387966823771667
32-33	0.021210684750347975
34-35	0.35614324835975736
36-37	0.5437936479660388
38-39	0.4962092505759343
40-41	0.5051162285138028
42-43	0.4526707346898107
44-45	0.4255270968384757
46-47	0.4244854333169284
48-49	0.41376686664593404
50-51	0.41062677950851595
52-53	0.47480231037949766
54-55	0.4753910767177636
56-57	0.44406568820746317
58-59	0.428712473694222
60-61	0.44317499041367625
62-63	0.42219075425496905
64-65	0.4738663228673826
66-67	0.4958016431109809
68-69	0.43571728346230837
70-71	0.39053324114359556
72-73	0.37696142221792805
74-75	0.3587398588772377
76-77	0.3568376907074556
78-79	0.35609795864142924
80-81	0.3730363132961555
82-83	0.36627304869248584
84-85	0.367888381979523
86-87	0.3532447063867561
88-89	0.3982777829777084
90-91	0.3745912602920885
92-93	0.3658201515092044
94-95	0.37280986470451477
96-97	0.37223619493902493
98	0.3685375346088931
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3312010.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.478724954671115
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.74112890074564	47.42900673462425
2	11.11479657075518	13.22187856318138
3	3.7553939677341877	6.700981347098785
4	1.7326685796856114	4.1222767155488445
5	0.9725411327069922	2.892275326969174
6	0.5683525293690082	2.028293026297852
7	0.3916069175374592	1.6304596096989972
8	0.28276268656066844	1.3454691249108701
9	0.20100634199468778	1.0760040836681932
>10	1.1278750992654285	12.091830060349357
>50	0.0751345814961703	3.0364235898945013
>100	0.03499307893457128	3.5033973602873325
>500	0.0011767971766834745	0.46865812646045646
>1k	5.628160378006584E-4	0.4530463310100393
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.0386291104193526E-5
2	0.0	0.0	0.0	0.0	6.0386291104193526E-5
3	0.0	0.0	0.0	3.0193145552096763E-5	6.0386291104193526E-5
4	0.0	0.0	0.0	9.057943665629028E-5	6.0386291104193526E-5
5	0.0	0.0	0.0	9.057943665629028E-5	9.057943665629028E-5
6	0.0	0.0	0.0	9.057943665629028E-5	9.057943665629028E-5
7	0.0	0.0	0.0	9.057943665629028E-5	9.057943665629028E-5
8	0.0	0.0	0.0	9.057943665629028E-5	9.057943665629028E-5
9	0.0	0.0	0.0	1.5096572776048382E-4	9.057943665629028E-5
10-11	0.0	0.0	0.0	1.5096572776048382E-4	9.057943665629028E-5
12-13	0.0	0.0	0.0	1.660623005365322E-4	2.1135201886467734E-4
14-15	0.0	1.5096572776048382E-5	0.0	1.9625544608862895E-4	3.019314555209676E-4
16-17	0.0	3.0193145552096763E-5	0.0	2.1135201886467734E-4	3.6231774662516113E-4
18-19	0.0	3.0193145552096763E-5	0.0	2.2644859164072572E-4	3.925108921772579E-4
20-21	0.0	3.0193145552096763E-5	0.0	3.6231774662516113E-4	4.378006105054031E-4
22-23	0.0	3.0193145552096763E-5	0.0	5.132834743856449E-4	4.5289718328145144E-4
24-25	0.0	3.0193145552096763E-5	0.0	7.699252115784675E-4	4.5289718328145144E-4
26-27	0.0	3.0193145552096763E-5	0.0	0.001736105869245564	5.132834743856449E-4
28-29	0.0	3.0193145552096763E-5	0.0	0.007216161786951126	5.434766199377418E-4
30-31	3.0193145552096763E-5	3.0193145552096763E-5	0.0	0.03248782461405612	5.434766199377418E-4
32-33	3.0193145552096763E-5	3.0193145552096763E-5	0.0	0.08876784792316447	5.736697654898385E-4
34-35	3.0193145552096763E-5	6.0386291104193526E-5	0.0	0.16533766504328187	6.038629110419353E-4
36-37	3.0193145552096763E-5	6.0386291104193526E-5	0.0	0.28546109462229885	6.038629110419353E-4
38-39	3.0193145552096763E-5	7.54828638802419E-5	0.0	0.48974791742778556	6.189594838179836E-4
40-41	3.0193145552096763E-5	9.057943665629028E-5	0.0	0.7225219730616755	6.34056056594032E-4
42-43	3.0193145552096763E-5	1.2077258220838705E-4	0.0	0.9695320968233792	6.34056056594032E-4
44-45	3.0193145552096763E-5	1.2077258220838705E-4	0.0	1.2658023375533287	6.793457749221771E-4
46-47	3.0193145552096763E-5	1.2077258220838705E-4	0.0	1.6156503150654737	7.397320660263707E-4
48-49	3.0193145552096763E-5	1.2077258220838705E-4	0.0	1.9891697186904629	7.699252115784675E-4
50-51	3.0193145552096763E-5	1.2077258220838705E-4	0.0	2.4064389902204404	8.454080754587093E-4
52-53	3.0193145552096763E-5	1.2077258220838705E-4	0.0	2.8333851648998643	8.454080754587093E-4
54-55	3.0193145552096763E-5	1.2077258220838705E-4	0.0	3.3095159736836544	8.454080754587093E-4
56-57	3.0193145552096763E-5	1.2077258220838705E-4	0.0	3.859016126159039	8.454080754587093E-4
58-59	3.0193145552096763E-5	1.2077258220838705E-4	0.0	4.433168981977712	8.756012210108062E-4
60-61	3.0193145552096763E-5	1.2077258220838705E-4	0.0	5.040171980157065	8.756012210108062E-4
62-63	3.0193145552096763E-5	1.2077258220838705E-4	0.0	5.663418890643446	9.057943665629029E-4
64-65	3.0193145552096763E-5	1.2077258220838705E-4	0.0	6.326898167577996	9.057943665629029E-4
66-67	3.0193145552096763E-5	1.5096572776048382E-4	0.0	7.075914625861637	9.057943665629029E-4
68-69	3.0193145552096763E-5	1.5096572776048382E-4	0.0	7.895779300183272	9.057943665629029E-4
70-71	3.0193145552096763E-5	1.5096572776048382E-4	0.0	8.753928883064967	9.057943665629029E-4
72-73	3.0193145552096763E-5	1.5096572776048382E-4	0.0	9.624170820740275	9.208909393389512E-4
74-75	3.0193145552096763E-5	1.5096572776048382E-4	0.0	10.512407873164634	9.359875121149996E-4
76-77	3.0193145552096763E-5	1.5096572776048382E-4	0.0	11.47377272411617	9.359875121149996E-4
78-79	3.0193145552096763E-5	1.5096572776048382E-4	0.0	12.496173018801272	9.359875121149996E-4
80-81	3.0193145552096763E-5	1.5096572776048382E-4	0.0	13.565659523974867	9.51084084891048E-4
82-83	3.0193145552096763E-5	1.5096572776048382E-4	0.0	14.642618832672607	9.963738032191932E-4
84-85	3.0193145552096763E-5	1.5096572776048382E-4	0.0	15.722763518226092	9.963738032191932E-4
86	3.0193145552096763E-5	1.5096572776048382E-4	0.0	16.554056298139198	9.963738032191932E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2530	0.0	48.74986	1
GTATCAA	4875	0.0	44.086063	1
TCAACGC	6230	0.0	34.00239	4
ATCAACG	6375	0.0	33.44478	3
CAACGCA	6500	0.0	32.30782	5
TATCAAC	6800	0.0	31.085701	2
AACGCAG	6960	0.0	30.69493	6
GTACATG	9950	0.0	30.526707	1
TACATGG	10385	0.0	28.258047	2
ACATGGG	10455	0.0	27.848719	3
ACGCAGA	8390	0.0	24.80455	7
CATGGGG	7170	0.0	24.172869	4
CGCAGAG	8580	0.0	24.040476	8
GCAGAGT	9495	0.0	20.999336	9
GAGTACA	7275	0.0	19.357422	1
GTATATA	1110	0.0	18.242685	1
AGTACAT	7515	0.0	17.755491	2
AGAGTAC	8380	0.0	17.722916	10-11
ATGGGGA	3730	0.0	17.209757	5
TAAGGCG	1070	0.0	17.140825	5
>>END_MODULE