FastQCFastQC Report
Mon 15 Aug 2016
SRR2920853_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920853_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3312010
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26750.049.2402041
GTATCAA51050.044.1516041
TCAACGC64950.033.468924
ATCAACG66100.033.0302433
CAACGCA66950.032.3938035
AACGCAG70750.030.9226956
TATCAAC72950.030.4487442
GTACATG101200.026.8299261
ACGCAGA85650.025.2096737
TACATGG106000.024.7609942
CGCAGAG88350.024.384448
ACATGGG107150.024.0526583
GAGTACA81850.021.3211821
CATGGGG74100.021.2014314
TAAGGTG11450.020.3089225
GCAGAGT102300.019.714099
AGTACAT81200.019.5811252
AGAGTAC87950.018.85581210-11
GTATAGA8100.018.785761
GTGGTAT16950.017.1129151
Produced by FastQC (version 0.11.5)