##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920853_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3312010
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.215877971382938	31.0	30.0	34.0	26.0	34.0
2	30.231385170938495	31.0	30.0	34.0	26.0	34.0
3	30.37027364047814	31.0	30.0	34.0	26.0	34.0
4	34.006846899616846	35.0	35.0	37.0	30.0	37.0
5	33.52656966615439	35.0	35.0	37.0	28.0	37.0
6	33.47059066850644	35.0	35.0	37.0	28.0	37.0
7	33.28770716271992	35.0	33.0	37.0	27.0	37.0
8	33.21978103930846	35.0	33.0	37.0	27.0	37.0
9	34.542226019848975	38.0	34.0	39.0	27.0	39.0
10-11	34.35530448277632	37.5	34.0	39.0	27.0	39.0
12-13	34.17039546982044	37.0	34.0	39.0	26.0	39.0
14-15	35.06708584817075	38.0	34.0	40.0	26.0	41.0
16-17	34.944008170265185	38.0	34.0	40.0	25.0	41.0
18-19	34.85217118909665	38.0	33.5	40.0	25.0	41.0
20-21	34.73735058167095	38.0	34.0	40.0	25.0	41.0
22-23	34.58939420472764	38.0	33.5	40.0	24.0	41.0
24-25	34.40359962681272	38.0	33.0	40.0	23.5	41.0
26-27	34.22149389645563	38.0	33.0	40.0	23.0	41.0
28-29	34.166220814550684	38.0	33.0	40.0	22.5	41.0
30-31	34.15596963777283	38.0	33.0	40.0	22.5	41.0
32-33	34.00690758783941	38.0	33.0	40.0	21.5	41.0
34-35	33.83672377196929	38.0	33.0	40.0	20.0	41.0
36-37	33.639252296943546	38.0	32.5	40.0	18.5	41.0
38-39	33.460430524062424	38.0	32.0	40.0	18.0	41.0
40-41	33.22499056464201	38.0	32.0	40.0	16.0	41.0
42-43	33.24929921709173	38.0	32.0	40.0	16.0	41.0
44-45	33.10473005818219	38.0	32.0	40.0	15.0	41.0
46-47	32.858334666863925	37.5	31.5	40.0	13.5	41.0
48-49	32.593909740610684	37.0	31.0	40.0	9.0	41.0
50-51	32.33406556743488	37.0	31.0	40.0	8.5	41.0
52-53	32.07467655592828	37.0	31.0	40.0	8.0	41.0
54-55	31.795290926053966	36.0	30.0	40.0	7.0	41.0
56-57	31.47791990362348	36.0	30.0	39.0	2.0	41.0
58-59	31.146339684964722	35.5	29.0	39.0	2.0	40.5
60-61	30.822639877295057	35.0	29.0	39.0	2.0	40.0
62-63	30.506287722561225	35.0	28.5	39.0	2.0	40.0
64-65	30.10575270002204	35.0	28.0	38.0	2.0	40.0
66-67	29.679085510007518	34.0	28.0	38.0	2.0	40.0
68-69	29.241518141551506	34.0	28.0	37.0	2.0	39.5
70-71	28.817683974384135	34.0	26.5	36.5	2.0	39.0
72-73	28.370834478156766	34.0	26.0	36.0	2.0	39.0
74-75	27.930130645740803	33.0	26.0	35.5	2.0	38.5
76-77	26.468289799849636	31.5	24.5	34.5	2.0	36.0
78-79	27.309403806147927	33.0	26.0	35.0	2.0	37.0
80-81	27.33000262680366	33.0	26.0	35.0	2.0	36.5
82-83	26.987474977430622	33.0	25.0	35.0	2.0	36.0
84-85	26.665258559001934	33.0	25.0	35.0	2.0	36.0
86-87	26.114730631851955	32.0	24.0	35.0	2.0	35.0
88-89	25.747235817524704	32.0	22.0	35.0	2.0	35.0
90-91	25.373950863674928	32.0	20.0	34.5	2.0	35.0
92-93	25.059762198785634	32.0	19.5	34.0	2.0	35.0
94-95	24.67523709167545	32.0	17.0	34.0	2.0	35.0
96-97	24.132119770169776	31.0	4.5	34.0	2.0	35.0
98-99	23.515419639433453	31.0	2.0	34.0	2.0	35.0
100-101	21.30360219323009	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	78835.0
3	36190.0
4	19300.0
5	13577.0
6	11838.0
7	11533.0
8	13172.0
9	15285.0
10	17358.0
11	19332.0
12	21177.0
13	23217.0
14	25228.0
15	26660.0
16	28606.0
17	29567.0
18	28895.0
19	27976.0
20	27665.0
21	28831.0
22	31020.0
23	35249.0
24	40795.0
25	48451.0
26	57609.0
27	67833.0
28	80464.0
29	94357.0
30	111179.0
31	131569.0
32	157866.0
33	192879.0
34	242302.0
35	313545.0
36	416098.0
37	488230.0
38	278432.0
39	19889.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.6723798898095	19.08207050691746	13.172732630111557	25.072816973161483
2	16.307015981231938	20.763403492139215	37.73747663805363	25.192103888575218
3	19.35060044757645	24.198509910427333	29.034310470456543	27.416579171539667
4	13.84189321323592	16.236674604579026	35.719186334792276	34.20224584739278
5	14.594855251095778	36.829232647825776	33.96852591518097	14.607386185897475
6	34.01113102332207	34.95490735582732	16.83291465298598	14.201046967864633
7	29.741388824467997	31.339668721046504	20.655119687304502	18.263822767181
8	26.836087994369755	34.579460724918434	19.840542854447968	18.743908426263843
9	26.91798667828162	15.680972995233777	19.24888222201594	38.15215810446866
10-11	25.84937046499621	25.38169300359475	27.426654003015578	21.34228252839346
12-13	26.479013481665557	22.474853464592623	27.46781102906314	23.578322024678677
14-15	23.348842791109913	24.137288930255504	24.836363229647088	27.6775050489875
16-17	23.708514608818845	27.35054110345313	25.55106262092061	23.389881666807412
18-19	23.65297149982451	26.372917422421242	27.033307794431533	22.940803283322715
20-21	24.338292727276023	25.755809703771405	26.696078899247723	23.209818669704845
22-23	24.04040455931543	25.50534407153915	26.655341608694116	23.798909760451302
24-25	23.90749912539508	25.78647978620366	26.694543250799228	23.61147783760203
26-27	23.588942156987894	26.069033341365095	26.585352036503963	23.75667246514305
28-29	23.612043295791548	26.121798260266498	26.657045111160382	23.609113332781572
30-31	23.796523795149657	25.867227838148377	26.499013867763	23.83723449893897
32-33	23.63956598763576	25.95685277156391	26.401668781134667	24.00191245966566
34-35	24.054991950342476	25.985762177692827	26.251788938613924	23.70745693335077
36-37	24.112949123938492	25.604921183834822	26.437863289073345	23.84426640315334
38-39	23.886967631255455	25.7928182886276	26.52660446825797	23.793609611858976
40-41	24.033545328429316	25.58288081085746	26.553431477141782	23.830142383571445
42-43	23.933236511196124	25.510750865790115	26.430072981988456	24.125939641025308
44-45	23.728281705335192	25.722337441042846	26.468405730920715	24.080975122701247
46-47	23.69161797958805	25.7578066326986	26.35346856831069	24.197106819402663
48-49	23.717103529887435	25.735486948002624	26.339958713477785	24.207450808632153
50-51	23.659073888412433	25.798676120646963	26.318096440262362	24.224153550678245
52-53	23.848635441030556	25.62078661354697	26.333472835943116	24.197105109479352
54-55	23.411779502393333	25.458091675860384	26.578353640538094	24.551775181208193
56-57	23.316250542565438	25.72929475928966	26.50646738002227	24.447987318122628
58-59	23.34809736348984	25.42305302308841	26.483523849610403	24.745325763811348
60-61	23.282979841137273	25.45042640582854	26.704600278416724	24.561993474617463
62-63	23.163298105959324	25.497221724404547	26.61348413711303	24.725996032523103
64-65	23.00021438622131	25.409477682696313	26.509475267076922	25.08083266400546
66-67	22.9698291420554	25.59958859980457	26.346739079544925	25.083843178595107
68-69	23.033965758328247	25.37527096481152	26.46490233178574	25.12586094507449
70-71	22.867233973799983	25.351411057581597	26.375402698219634	25.405952270398785
72-73	22.954351376684134	25.419940692222116	26.266965954363158	25.35874197673059
74-75	22.78125646414401	25.579568824879246	26.068293997844442	25.5708807131323
76-77	22.788271803291142	25.420588020277645	26.279979917906797	25.511160258524406
78-79	22.777851223936164	25.499270616693398	26.1585938426602	25.564284316710246
80-81	22.618886021890486	25.70019041573624	26.029322302075066	25.651601260298207
82-83	22.663304147581634	25.66272633099756	25.98282796149117	25.691141559929637
84-85	22.54551417195137	25.507353337519724	25.937200808026876	26.009931682502025
86-87	22.54148570906089	25.493514894194202	25.973575761649293	25.99142363509561
88-89	22.381793044772945	25.484586985916348	25.900761155254404	26.232858814056303
90-91	22.23369815809463	25.56338132639348	25.930284976265856	26.272635539246036
92-93	22.332918204461574	25.413780460459733	25.871407014162862	26.381894320915826
94-95	22.117318162494325	25.31212560024734	25.96558903950763	26.6049671977507
96-97	22.229678582719153	25.19664913675475	25.87751969208871	26.696152588437382
98-99	22.06978451218507	25.263905919325	25.794676801491356	26.87163276699857
100-101	22.029091451498747	25.148845110500773	25.931327434483393	26.890736003517084
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	62.0
1	49.5
2	62.5
3	109.5
4	108.5
5	120.5
6	211.5
7	396.0
8	601.5
9	661.5
10	563.0
11	486.0
12	518.5
13	582.5
14	652.5
15	739.5
16	834.5
17	915.5
18	1019.5
19	1173.5
20	1310.5
21	1531.5
22	1857.5
23	2278.0
24	2798.0
25	3399.5
26	4219.0
27	5514.5
28	7284.5
29	10164.0
30	14641.0
31	20890.0
32	29089.5
33	36220.0
34	42745.5
35	50497.5
36	59262.5
37	67603.0
38	74242.0
39	79724.5
40	85412.5
41	92563.5
42	100874.0
43	109328.5
44	115427.0
45	121721.5
46	129034.0
47	135139.5
48	141429.0
49	147205.0
50	149195.5
51	149492.5
52	148402.0
53	145507.5
54	141338.0
55	132987.5
56	120771.5
57	108107.0
58	97299.5
59	84959.5
60	70770.0
61	58111.5
62	47944.5
63	37681.0
64	27816.0
65	21069.5
66	16190.0
67	12596.0
68	9512.0
69	6907.0
70	5394.5
71	4313.0
72	3495.5
73	2843.5
74	2130.5
75	1587.5
76	1082.0
77	614.5
78	328.0
79	152.5
80	75.5
81	40.0
82	14.5
83	8.0
84	8.0
85	8.0
86	4.5
87	1.5
88	1.0
89	1.0
90	1.5
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1951081065576493
2	0.0
3	0.011986678784182414
4	0.014371937282798059
5	0.006219787983731934
6	0.10866513084199625
7	0.14393072484684527
8	0.12566387178782673
9	0.14429304259347042
10-11	0.11636438295778093
12-13	0.11625870694834858
14-15	0.11838732370977141
16-17	0.06779870833723328
18-19	0.08090253350684327
20-21	0.037922590813433535
22-23	0.08541640876688174
24-25	0.0586954749532761
26-27	0.06740619744505602
28-29	0.041908086026310305
30-31	0.025407531982089424
32-33	0.032925625224561515
34-35	0.02080307728539467
36-37	0.02583023601981878
38-39	0.03321246010730644
40-41	0.025618884000954106
42-43	0.013269887470146529
44-45	0.027747500762376923
46-47	0.034888179685447815
48-49	0.008318211599602658
50-51	0.016002367142611284
52-53	0.00736712751471161
54-55	0.012635831413552496
56-57	0.005963146246539111
58-59	0.003985495212876773
60-61	0.0237469089767241
62-63	0.01006941404162427
64-65	0.006944423476982256
66-67	0.04121364367861208
68-69	0.09060962980184238
70-71	0.022720342027952815
72-73	0.044776434853759504
74-75	0.0872732872183357
76-77	0.2587401608086932
78-79	0.08174794158230199
80-81	0.0172553826830233
82-83	0.012243320521375237
84-85	0.014688965311095075
86-87	0.2746217553690961
88-89	0.6328483307719481
90-91	0.2706211635834433
92-93	0.35718491188130475
94-95	0.001268112113188064
96-97	0.0044383923961582245
98-99	0.0038949157762204825
100-101	0.0053743799082732234
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3312010.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.496775474972885
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.25177478641746	44.992247254127875
2	11.883014152458996	13.6646999337972
3	4.0600630421422235	7.003215994448604
4	1.8510705349698688	4.25722347750002
5	1.0199693126771168	2.9322473281179295
6	0.6271187014065366	2.1634381902556914
7	0.42876752563522463	1.7256925106685732
8	0.3053409330336643	1.404489525596426
9	0.22680115231708559	1.1736301439016534
>10	1.2208429354330372	12.693758172051487
>50	0.08539010257779893	3.3420015655090367
>100	0.037841280799167445	3.668204528938334
>500	0.0013194342991996962	0.4841287094161854
>1k	6.861058325736837E-4	0.49502266567100045
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.0193145552096763E-5	0.0
3	0.0	0.0	0.0	3.0193145552096763E-5	0.0
4	0.0	0.0	0.0	3.0193145552096763E-5	0.0
5	0.0	0.0	0.0	3.0193145552096763E-5	3.0193145552096763E-5
6	0.0	0.0	0.0	3.0193145552096763E-5	3.0193145552096763E-5
7	0.0	0.0	0.0	9.057943665629028E-5	3.0193145552096763E-5
8	0.0	0.0	0.0	9.057943665629028E-5	3.0193145552096763E-5
9	0.0	0.0	0.0	9.057943665629028E-5	3.0193145552096763E-5
10-11	0.0	0.0	0.0	9.057943665629028E-5	3.0193145552096763E-5
12-13	0.0	0.0	0.0	1.2077258220838705E-4	1.509657277604838E-4
14-15	0.0	0.0	0.0	1.660623005365322E-4	2.415451644167741E-4
16-17	0.0	0.0	0.0	1.8115887331258057E-4	3.0193145552096765E-4
18-19	0.0	0.0	0.0	2.5664173719282246E-4	3.6231774662516113E-4
20-21	0.0	0.0	0.0	3.6231774662516113E-4	4.0760746495330626E-4
22-23	0.0	0.0	0.0	5.283800471616933E-4	4.5289718328145144E-4
24-25	0.0	1.5096572776048382E-5	0.0	8.001183571305642E-4	4.5289718328145144E-4
26-27	0.0	3.0193145552096763E-5	0.0	0.001781395587573709	4.830903288335482E-4
28-29	0.0	3.0193145552096763E-5	0.0	0.007291644650831368	4.830903288335482E-4
30-31	0.0	3.0193145552096763E-5	0.0	0.03226137602241539	4.830903288335482E-4
32-33	0.0	3.0193145552096763E-5	0.0	0.08783186041104948	4.830903288335482E-4
34-35	0.0	3.0193145552096763E-5	0.0	0.16385820091122913	5.434766199377418E-4
36-37	0.0	4.528971832814514E-5	0.0	0.28349854016141257	5.434766199377418E-4
38-39	0.0	6.0386291104193526E-5	0.0	0.48724188634696153	5.434766199377418E-4
40-41	0.0	6.0386291104193526E-5	0.0	0.7216010821223366	5.434766199377418E-4
42-43	0.0	6.0386291104193526E-5	0.0	0.9706945329271349	5.434766199377418E-4
44-45	0.0	6.0386291104193526E-5	0.0	1.2676894091503348	5.434766199377418E-4
46-47	0.0	6.0386291104193526E-5	0.0	1.6163900471315	5.434766199377418E-4
48-49	0.0	6.0386291104193526E-5	0.0	1.985848472679732	5.434766199377418E-4
50-51	0.0	6.0386291104193526E-5	0.0	2.3974263362731394	5.434766199377418E-4
52-53	0.0	6.0386291104193526E-5	0.0	2.819058517335394	5.434766199377418E-4
54-55	0.0	6.0386291104193526E-5	0.0	3.28756555686728	5.434766199377418E-4
56-57	0.0	6.0386291104193526E-5	0.0	3.8296683886824012	5.585731927137902E-4
58-59	0.0	6.0386291104193526E-5	0.0	4.399337562386587	6.038629110419353E-4
60-61	0.0	6.0386291104193526E-5	0.0	4.999547102816718	6.038629110419353E-4
62-63	0.0	6.0386291104193526E-5	0.0	5.616045845272206	6.038629110419353E-4
64-65	0.0	6.0386291104193526E-5	0.0	6.270603047696111	6.038629110419353E-4
66-67	0.0	1.2077258220838705E-4	0.0	7.011180521797941	6.038629110419353E-4
68-69	0.0	1.3586915498443544E-4	0.0	7.81785079151331	6.038629110419353E-4
70-71	0.0	1.8115887331258057E-4	0.0	8.664345820211896	6.038629110419353E-4
72-73	0.0	2.1135201886467734E-4	0.0	9.523537066615138	6.491526293700804E-4
74-75	0.0	2.1135201886467734E-4	0.0	10.403667259458757	7.095389204742739E-4
76-77	0.0	2.1135201886467734E-4	0.0	11.359340702473725	7.246354932503223E-4
78-79	0.0	2.1135201886467734E-4	0.0	12.37369452386919	7.548286388024191E-4
80-81	0.0	2.2644859164072572E-4	0.0	13.433715477912205	7.548286388024191E-4
82-83	0.0	2.415451644167741E-4	0.0	14.500167571957814	7.699252115784675E-4
84-85	0.0	2.415451644167741E-4	0.0	15.571420376146207	7.850217843545158E-4
86-87	0.0	2.415451644167741E-4	0.0	16.679493721335383	8.152149299066125E-4
88-89	0.0	2.415451644167741E-4	0.0	17.84944791833358	8.454080754587093E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2675	0.0	49.240204	1
GTATCAA	5105	0.0	44.151604	1
TCAACGC	6495	0.0	33.46892	4
ATCAACG	6610	0.0	33.030243	3
CAACGCA	6695	0.0	32.393803	5
AACGCAG	7075	0.0	30.922695	6
TATCAAC	7295	0.0	30.448744	2
GTACATG	10120	0.0	26.829926	1
ACGCAGA	8565	0.0	25.209673	7
TACATGG	10600	0.0	24.760994	2
CGCAGAG	8835	0.0	24.38444	8
ACATGGG	10715	0.0	24.052658	3
GAGTACA	8185	0.0	21.321182	1
CATGGGG	7410	0.0	21.201431	4
TAAGGTG	1145	0.0	20.308922	5
GCAGAGT	10230	0.0	19.71409	9
AGTACAT	8120	0.0	19.581125	2
AGAGTAC	8795	0.0	18.855812	10-11
GTATAGA	810	0.0	18.78576	1
GTGGTAT	1695	0.0	17.112915	1
>>END_MODULE