FastQCFastQC Report
Mon 15 Aug 2016
SRR2920852_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920852_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3382621
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52350.044.3992421
GGTATCA28750.038.1013151
TCAACGC62650.036.377044
ATCAACG64500.034.97823
CAACGCA64750.034.9139675
TATCAAC67950.033.540182
AACGCAG68550.033.173346
ACGCAGA79950.027.863967
CGCAGAG84200.026.1837838
GTACATG98200.025.4032141
TACATGG101750.023.9308742
ACATGGG103350.023.1163523
GCAGAGT99450.021.6152889
TAAGGCG8000.021.2081995
CATGGGG70850.018.9635584
AGAGTAC82950.018.3179210-11
GTATTAA17800.018.1000161
GAGTACA75950.017.089221
CAGAGTA90250.016.60770410-11
GTATAGC13650.016.5220641
Produced by FastQC (version 0.11.5)