##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920852_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3382621
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.663876029859686	31.0	30.0	33.0	22.0	34.0
2	28.698308501011493	31.0	28.0	34.0	20.0	34.0
3	28.77443999785965	31.0	28.0	34.0	22.0	34.0
4	32.26203556354673	35.0	32.0	37.0	23.0	37.0
5	32.230836679604366	35.0	32.0	37.0	23.0	37.0
6	32.34902284352873	35.0	33.0	37.0	24.0	37.0
7	32.19194730949758	35.0	32.0	37.0	23.0	37.0
8	32.133540234037454	35.0	32.0	37.0	22.0	37.0
9	33.403724212674135	37.0	33.0	39.0	22.0	39.0
10-11	33.213597089357634	37.0	33.0	39.0	20.0	39.0
12-13	33.04650949662998	37.0	32.0	39.0	18.5	39.0
14-15	33.81435032183623	38.0	33.0	40.0	18.0	41.0
16-17	33.69947505203805	38.0	32.5	40.0	17.5	41.0
18-19	33.58787061867115	38.0	32.0	40.0	17.5	41.0
20-21	33.49733209839353	38.0	32.0	40.0	18.0	41.0
22-23	33.2728415332371	38.0	32.0	40.0	16.5	41.0
24-25	33.08341076342872	38.0	32.0	40.0	15.5	41.0
26-27	33.122150693205064	38.0	32.0	40.0	14.0	41.0
28-29	32.97011769867213	38.0	32.0	40.0	10.5	41.0
30-31	32.72450579003678	38.0	31.5	40.0	9.5	41.0
32-33	32.48610160582578	37.5	31.0	40.0	9.0	41.0
34-35	32.162376748680984	37.0	30.5	40.0	8.0	41.0
36-37	31.864559316577292	37.0	30.0	40.0	7.0	41.0
38-39	31.665311307415166	37.0	30.0	40.0	2.0	41.0
40-41	31.470667272508507	37.0	30.0	40.0	2.0	41.0
42-43	31.24865392841823	37.0	30.0	40.0	2.0	41.0
44-45	30.97385474163378	36.0	29.5	39.5	2.0	41.0
46-47	30.74001639557018	36.0	29.0	39.0	2.0	40.0
48-49	30.438304793827037	36.0	28.0	39.0	2.0	40.0
50-51	29.315069290943324	34.0	26.5	38.0	2.0	39.5
52-53	29.771469372418608	35.0	27.5	38.5	2.0	39.5
54-55	30.42841438635898	36.0	28.0	39.0	2.0	40.0
56-57	30.385060578764218	36.0	28.0	39.0	2.0	41.0
58-59	30.153460290112314	35.0	28.0	39.0	2.0	41.0
60-61	29.835032508814912	35.0	28.0	39.0	2.0	40.0
62-63	29.447850793807525	35.0	27.5	39.0	2.0	40.0
64-65	29.03149199984273	34.0	26.0	38.0	2.0	40.0
66-67	28.65222397070201	34.0	26.0	38.0	2.0	40.0
68-69	28.214814636342645	34.0	26.0	37.0	2.0	40.0
70-71	27.891940894353816	34.0	26.0	37.0	2.0	39.0
72-73	27.428034946865168	33.5	25.0	36.0	2.0	39.0
74-75	26.982998538707115	33.0	24.0	36.0	2.0	39.0
76-77	26.552639210836805	33.0	22.5	35.0	2.0	37.5
78-79	26.084516414933866	32.5	21.5	35.0	2.0	37.0
80-81	25.625383984785763	32.0	20.0	35.0	2.0	36.5
82-83	25.14235543976106	32.0	18.0	35.0	2.0	36.0
84-85	24.72463616231319	32.0	15.0	35.0	2.0	35.5
86-87	24.25653051287744	31.0	7.5	34.0	2.0	35.0
88-89	23.709468190494885	31.0	2.0	34.0	2.0	35.0
90-91	23.20461559246513	31.0	2.0	34.0	2.0	35.0
92-93	22.657350320949348	30.5	2.0	34.0	2.0	35.0
94-95	22.008405464283463	30.0	2.0	34.0	2.0	35.0
96-97	21.353698507754785	29.5	2.0	34.0	2.0	35.0
98	20.947938891173443	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	168832.0
3	28900.0
4	21307.0
5	17007.0
6	16107.0
7	16341.0
8	18495.0
9	21338.0
10	23152.0
11	24998.0
12	26065.0
13	25607.0
14	24423.0
15	25365.0
16	27068.0
17	28720.0
18	30175.0
19	30363.0
20	31531.0
21	33596.0
22	36374.0
23	41128.0
24	47007.0
25	54026.0
26	62085.0
27	72238.0
28	83885.0
29	97173.0
30	113296.0
31	134126.0
32	159637.0
33	193760.0
34	240244.0
35	304606.0
36	386338.0
37	429424.0
38	262088.0
39	25795.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.86052917465613	18.11396192364545	13.089444408166173	24.936064493532246
2	15.937394468196594	19.611424137455856	39.38019600896831	25.07098538537923
3	19.409860981130514	23.614076640010172	28.963205757684623	28.01285662117469
4	13.429722314459145	15.64291566713217	36.554680364317946	34.37268165409074
5	14.644587531695633	36.58702900104235	34.15259737859556	14.615786088666455
6	33.606099538868	34.78339806528588	17.430552988782917	14.1799494070632
7	29.291159735502863	31.287365661024047	21.003153055661013	18.41832154781208
8	26.585477988732336	34.314989008779676	20.15841549030304	18.941117512184956
9	26.73193361006155	16.020919872489408	19.61422813847605	37.63291837897299
10-11	25.60795312274121	25.19600924844965	27.780647018983206	21.41539060982593
12-13	26.3099683688852	22.560384859519594	27.449962733881478	23.679684037713724
14-15	23.441252080377865	24.198724700099643	25.036005189654926	27.32401802986757
16-17	23.660525612069183	27.109068576908196	25.682669950725806	23.547735860296807
18-19	23.592826537216542	26.481632718244136	27.00962728377575	22.915913460763573
20-21	24.385480835487478	25.737628623644532	26.771174102101263	23.105716438766727
22-23	23.97656518296762	25.535700202900323	26.55427076843041	23.933463845701652
24-25	23.77809181132704	25.724006911630585	26.643620009413116	23.854281267629258
26-27	23.651701366175754	25.958493132730908	26.55881520192638	23.830990299166956
28-29	23.775018646542748	26.05968047302097	26.459144659457113	23.706156220979167
30-31	23.929969030906978	25.873375749455008	26.324365015277497	23.872290204360517
32-33	23.67452820285821	25.89046600603387	26.26628924669469	24.16871654441323
34-35	24.119716272836854	25.89170567682765	26.261287333829173	23.727290716506317
36-37	24.159455117487692	25.64461069523644	26.233958854415135	23.96197533286074
38-39	23.985143654026523	25.822029258142305	26.249023683818834	23.943803404012336
40-41	24.116775716422207	25.631349877163967	26.416484347597823	23.835390058816007
42-43	24.01982474354162	25.53046735055568	26.291735364410844	24.157972541491855
44-45	23.926658584452728	25.70121766118621	26.267690110282587	24.104433644078476
46-47	24.0212390273808	25.57147427024243	26.1998870359251	24.207399666451675
48-49	23.838887376595093	25.60764772743802	26.222323415621574	24.33114148034531
50-51	23.78725036863552	25.794245352817768	26.309051254251276	24.109453024295437
52-53	23.9164002199791	25.591327488907112	26.37058101838204	24.121691272731756
54-55	23.590208939618627	25.49309883660056	26.559651047000088	24.357041176780726
56-57	23.577183385285558	25.650229275220088	26.484483742876208	24.288103596618143
58-59	23.55149124326658	25.424149752113866	26.551049313137852	24.473309691481703
60-61	23.55397331166046	25.382310308032263	26.735538619580623	24.328177760726653
62-63	23.413721350765694	25.434163873278802	26.71420398762488	24.43791078833062
64-65	23.419304679848132	25.28341732946508	26.619167454401126	24.67811053628566
66-67	23.351959439977932	25.404321987584872	26.562649241698054	24.681069330739135
68-69	23.478694756451166	25.26011089573043	26.543210334749283	24.71798401306912
70-71	23.30870157434617	25.156625132941606	26.60597985327194	24.928693439440288
72-73	23.30324815207668	25.22555725539228	26.60016976606201	24.871024826469025
74-75	23.345440218078203	25.33749844980054	26.518468586876736	24.798592745244523
76-77	23.372864935251037	25.152580650090066	26.638787311852553	24.835767102806344
78-79	23.378960793987833	25.275208113851676	26.51166799332232	24.834163098838168
80-81	23.37382488162023	25.23420767303861	26.535153702309078	24.856813743032077
82-83	23.478360850062572	25.257887275988455	26.56913601401688	24.694615859932096
84-85	23.491653036321626	25.175747999955494	26.522003271282813	24.810595692440074
86-87	23.50009619593651	25.173193478138007	26.556679431973752	24.77003089395173
88-89	23.479916662672146	25.030938367092354	26.5227323353017	24.9664126349338
90-91	23.41257795008524	25.078109583390827	26.587481398064362	24.921831068459575
92-93	23.57624638107947	24.984708368598415	26.58481459036008	24.854230659962038
94-95	23.509660921191	24.8870053814874	26.602428672147067	25.000905025174536
96-97	23.698926013824078	24.72680027845185	26.580816614056356	24.99345709366771
98	23.719692865149902	24.68991877744236	26.509073818350355	25.081314539057388
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	39.0
1	41.5
2	69.5
3	114.5
4	135.5
5	166.0
6	243.5
7	459.5
8	736.5
9	844.0
10	881.5
11	997.5
12	1168.0
13	1352.0
14	1521.5
15	1679.0
16	1758.0
17	1810.0
18	1833.5
19	1810.0
20	1833.5
21	1872.0
22	1999.5
23	2298.5
24	2715.0
25	3308.0
26	4329.5
27	5648.5
28	7601.5
29	10727.0
30	15352.5
31	22065.0
32	29629.5
33	37618.0
34	46602.0
35	56824.0
36	67968.0
37	78431.0
38	88129.0
39	97195.5
40	105393.5
41	112986.0
42	120995.5
43	129301.0
44	136437.5
45	142406.5
46	147627.0
47	151701.0
48	153899.0
49	152422.5
50	150398.0
51	147998.5
52	143985.5
53	140834.0
54	135107.0
55	127328.0
56	116999.5
57	105481.0
58	96226.5
59	84133.5
60	70598.0
61	58676.5
62	47987.0
63	37369.0
64	27571.5
65	21071.0
66	16296.0
67	12684.0
68	9884.0
69	7478.5
70	5577.0
71	4319.5
72	3478.0
73	2656.5
74	1977.0
75	1454.5
76	982.5
77	594.5
78	339.0
79	211.5
80	123.5
81	67.5
82	43.0
83	29.0
84	20.5
85	13.0
86	10.5
87	9.0
88	7.0
89	6.5
90	6.5
91	6.5
92	5.5
93	3.5
94	5.0
95	5.0
96	1.5
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4020846556560726
2	0.02775953912661218
3	0.020871389375280294
4	0.018624610915618393
5	0.02501019180097327
6	0.021462646864665005
7	0.01492925160696395
8	0.011115640800432564
9	0.0
10-11	0.0
12-13	0.005676071898093224
14-15	0.0036214521224813534
16-17	0.006548176694935673
18-19	0.03670230865355593
20-21	0.07987888681587443
22-23	0.09978948277090456
24-25	0.06598433581533374
26-27	0.07692259936895088
28-29	0.05816495551822093
30-31	0.054129623153170284
32-33	0.02104876662209571
34-35	0.3523303379243492
36-37	0.5388129500762869
38-39	0.4922809856617102
40-41	0.5012533180631232
42-43	0.4497400093004803
44-45	0.42332558096221834
46-47	0.42255694622601825
48-49	0.41192909285432805
50-51	0.40733206587436194
52-53	0.4711583118534415
54-55	0.4723703897066801
56-57	0.4417728146310213
58-59	0.4269322516474651
60-61	0.44257101224169065
62-63	0.4212709611866065
64-65	0.4700053597491412
66-67	0.49211838985212947
68-69	0.43405690439455086
70-71	0.3899490956864514
72-73	0.37513809557736444
74-75	0.35732646370965004
76-77	0.35553790980426125
78-79	0.35445886488613415
80-81	0.37057063147186753
82-83	0.36653529910681687
84-85	0.36609185598977834
86-87	0.35169473612326063
88-89	0.3968076825633141
90-91	0.37473899677202976
92-93	0.36402245477693185
94-95	0.3709845117144368
96-97	0.37128014045912916
98	0.36584057155678984
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3382621.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.72984872497709
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.79195051477913	44.51249788303927
2	12.38055225873408	14.542159225742516
3	4.9393872914513945	8.702684052630442
4	2.4412917688771683	5.735067851187512
5	1.3378804544913219	3.9286758352189457
6	0.8146785523592253	2.8707568883724375
7	0.5092205906372186	2.0934513779037127
8	0.3645283287599076	1.7126954883230467
9	0.2689287665105943	1.4214731197465695
>10	1.0909826051594695	10.673256544992242
>50	0.04329311060577647	1.712087393674056
>100	0.016244550173235277	1.5594406066691684
>500	7.074716447124764E-4	0.2707853470796815
>1k	3.5373581676858315E-4	0.26496838542041606
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.956287446923554E-5	0.0	0.0	0.0
3	0.0	2.956287446923554E-5	0.0	0.0	0.0
4	0.0	5.912574893847108E-5	0.0	2.956287446923554E-5	0.0
5	0.0	2.069401212846488E-4	0.0	2.956287446923554E-5	0.0
6	0.0	2.069401212846488E-4	0.0	2.956287446923554E-5	0.0
7	0.0	2.3650299575388433E-4	0.0	2.956287446923554E-5	0.0
8	0.0	2.3650299575388433E-4	0.0	2.956287446923554E-5	0.0
9	0.0	2.3650299575388433E-4	0.0	2.956287446923554E-5	0.0
10-11	0.0	2.6606587022311986E-4	0.0	7.390718617308886E-5	0.0
12-13	0.0	3.547544936308265E-4	0.0	1.7737724681541326E-4	0.0
14-15	0.0	3.695359308654443E-4	0.0	2.2172155851926656E-4	0.0
16-17	0.0	3.8431736810006206E-4	0.0	2.3650299575388433E-4	0.0
18-19	0.0	3.8431736810006206E-4	0.0	2.3650299575388433E-4	0.0
20-21	0.0	5.616946149154753E-4	0.0	2.512844329885021E-4	0.0
22-23	0.0	5.764760521500931E-4	0.0	3.25191619161591E-4	0.0
24-25	0.0	5.912574893847108E-4	0.0	6.060389266193286E-4	0.0
26-27	0.0	5.912574893847108E-4	0.0	0.0015224880351656304	0.0
28-29	0.0	5.912574893847108E-4	0.0	0.0058830120193778725	0.0
30-31	0.0	6.208203638539464E-4	0.0	0.02564579360206183	0.0
32-33	0.0	6.208203638539464E-4	0.0	0.07204472508152701	0.0
34-35	2.956287446923554E-5	6.356018010885642E-4	0.0	0.13289990217644837	0.0
36-37	2.956287446923554E-5	6.651646755577998E-4	0.0	0.2339753699867647	0.0
38-39	2.956287446923554E-5	6.947275500270352E-4	0.0	0.3944426526057752	2.956287446923554E-5
40-41	2.956287446923554E-5	7.09508987261653E-4	0.0	0.5829651030960903	2.956287446923554E-5
42-43	2.956287446923554E-5	7.390718617308886E-4	0.0	0.7907921106148161	2.956287446923554E-5
44-45	2.956287446923554E-5	7.390718617308886E-4	0.0	1.0385585615414792	2.956287446923554E-5
46-47	2.956287446923554E-5	7.390718617308886E-4	0.0	1.335990641576458	2.956287446923554E-5
48-49	2.956287446923554E-5	7.390718617308886E-4	0.0	1.653643727748394	2.956287446923554E-5
50-51	2.956287446923554E-5	7.390718617308886E-4	0.0	1.9909561254423713	2.956287446923554E-5
52-53	2.956287446923554E-5	7.981976106693596E-4	0.0	2.3360139962472886	2.956287446923554E-5
54-55	2.956287446923554E-5	0.001108607792596333	0.0	2.729096756627479	2.956287446923554E-5
56-57	2.956287446923554E-5	0.0011825149787694216	0.0	3.1853701611856606	2.956287446923554E-5
58-59	5.912574893847108E-5	0.0013303293511155994	0.0	3.6735123444216775	2.956287446923554E-5
60-61	5.912574893847108E-5	0.001359892225584835	0.0	4.185083105674565	4.4344311703853315E-5
62-63	5.912574893847108E-5	0.0013746736628194528	0.0	4.710844046672683	5.912574893847108E-5
64-65	5.912574893847108E-5	0.0014485808489925415	0.0	5.27695831132131	7.390718617308886E-5
66-67	5.912574893847108E-5	0.0015077065979310126	0.0	5.930637810147811	8.868862340770663E-5
68-69	5.912574893847108E-5	0.0015077065979310126	0.0	6.635002857251818	8.868862340770663E-5
70-71	5.912574893847108E-5	0.0015372694724002482	0.0	7.36483632071107	8.868862340770663E-5
72-73	5.912574893847108E-5	0.0015668323468694836	0.0	8.115349014861552	8.868862340770663E-5
74-75	5.912574893847108E-5	0.0015668323468694836	0.0	8.893990784069512	8.868862340770663E-5
76-77	5.912574893847108E-5	0.0015668323468694836	0.0	9.742075745405707	8.868862340770663E-5
78-79	5.912574893847108E-5	0.0016111766585733371	0.0	10.657992722211564	8.868862340770663E-5
80-81	5.912574893847108E-5	0.0016998652819810436	0.0	11.600398034541854	8.868862340770663E-5
82-83	5.912574893847108E-5	0.0017146467192156614	0.0	12.567739631486944	8.868862340770663E-5
84-85	5.912574893847108E-5	0.0017294281564502792	0.0	13.54742372852294	8.868862340770663E-5
86	5.912574893847108E-5	0.0017737724681541325	0.0	14.30624359039928	8.868862340770663E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5235	0.0	44.399242	1
GGTATCA	2875	0.0	38.101315	1
TCAACGC	6265	0.0	36.37704	4
ATCAACG	6450	0.0	34.9782	3
CAACGCA	6475	0.0	34.913967	5
TATCAAC	6795	0.0	33.54018	2
AACGCAG	6855	0.0	33.17334	6
ACGCAGA	7995	0.0	27.86396	7
CGCAGAG	8420	0.0	26.183783	8
GTACATG	9820	0.0	25.403214	1
TACATGG	10175	0.0	23.930874	2
ACATGGG	10335	0.0	23.116352	3
GCAGAGT	9945	0.0	21.615288	9
TAAGGCG	800	0.0	21.208199	5
CATGGGG	7085	0.0	18.963558	4
AGAGTAC	8295	0.0	18.31792	10-11
GTATTAA	1780	0.0	18.100016	1
GAGTACA	7595	0.0	17.08922	1
CAGAGTA	9025	0.0	16.607704	10-11
GTATAGC	1365	0.0	16.522064	1
>>END_MODULE