##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920852_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3382621
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.02746272786694	31.0	30.0	34.0	26.0	34.0
2	30.04699905783119	31.0	30.0	34.0	26.0	34.0
3	30.189493295287885	31.0	30.0	34.0	26.0	34.0
4	33.81627560403604	35.0	35.0	37.0	30.0	37.0
5	33.30672250896568	35.0	33.0	37.0	27.0	37.0
6	33.24070801901839	35.0	33.0	37.0	27.0	37.0
7	33.04790456867618	35.0	33.0	37.0	26.0	37.0
8	32.97059114810675	35.0	33.0	37.0	26.0	37.0
9	34.28351979130976	38.0	34.0	39.0	27.0	39.0
10-11	34.079635732173365	37.0	33.5	39.0	26.0	39.0
12-13	33.88940366065249	37.0	33.0	39.0	25.0	39.0
14-15	34.75945354209058	38.0	33.0	40.0	25.0	41.0
16-17	34.627173129948645	38.0	33.0	40.0	24.5	41.0
18-19	34.524537037995096	38.0	33.0	40.0	24.0	41.0
20-21	34.407831087195405	38.0	33.0	40.0	23.0	41.0
22-23	34.25198226464035	38.0	33.0	40.0	22.5	41.0
24-25	34.0668910291753	38.0	33.0	40.0	21.0	41.0
26-27	33.86730245569929	38.0	33.0	40.0	19.0	41.0
28-29	33.80235681147843	38.0	32.5	40.0	18.5	41.0
30-31	33.79026145701809	38.0	33.0	40.0	19.0	41.0
32-33	33.637931651225486	38.0	32.5	40.0	18.0	41.0
34-35	33.45865365939607	38.0	32.0	40.0	17.0	41.0
36-37	33.25723839590661	38.0	32.0	40.0	16.0	41.0
38-39	33.06725007028574	38.0	32.0	40.0	15.5	41.0
40-41	32.83472431584857	37.5	31.0	40.0	14.5	41.0
42-43	32.86614107817577	38.0	31.0	40.0	14.0	41.0
44-45	32.7171357654316	37.5	31.0	40.0	9.0	41.0
46-47	32.468462325516214	37.0	31.0	40.0	9.0	41.0
48-49	32.20562427774202	37.0	31.0	40.0	8.0	41.0
50-51	31.9381702827482	37.0	30.0	40.0	7.0	41.0
52-53	31.675052570181528	36.5	30.0	40.0	2.0	41.0
54-55	31.38438595396883	36.0	30.0	39.5	2.0	41.0
56-57	31.054621992827457	36.0	29.0	39.0	2.0	40.5
58-59	30.71847082484263	35.0	29.0	39.0	2.0	40.0
60-61	30.38309568231262	35.0	28.0	39.0	2.0	40.0
62-63	30.05677446571756	35.0	28.0	38.5	2.0	40.0
64-65	29.6498389858042	34.5	28.0	38.0	2.0	40.0
66-67	29.213944009689527	34.0	27.5	38.0	2.0	40.0
68-69	28.76490035389717	34.0	26.0	37.0	2.0	39.5
70-71	28.33049194692518	34.0	26.0	36.5	2.0	39.0
72-73	27.883213785996126	33.0	26.0	36.0	2.0	39.0
74-75	27.428136790967713	33.0	25.0	35.5	2.0	38.5
76-77	26.009254066595105	31.0	23.0	34.0	2.0	36.0
78-79	26.83849772114582	32.5	25.0	35.0	2.0	37.0
80-81	26.858668766024927	33.0	25.0	35.0	2.0	36.5
82-83	26.51063982633585	33.0	24.0	35.0	2.0	36.0
84-85	26.172220298993	32.5	23.5	35.0	2.0	36.0
86-87	25.616107154777318	32.0	20.5	35.0	2.0	35.0
88-89	25.240419780992312	32.0	19.5	34.5	2.0	35.0
90-91	24.85244430280543	32.0	18.5	34.0	2.0	35.0
92-93	24.526088941090357	31.0	16.0	34.0	2.0	35.0
94-95	24.125595802781334	31.0	8.0	34.0	2.0	35.0
96-97	23.56480034269284	31.0	2.0	34.0	2.0	35.0
98-99	22.92305301125961	31.0	2.0	34.0	2.0	35.0
100-101	20.723468872214774	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	93747.0
3	40927.0
4	21919.0
5	15305.0
6	13035.0
7	13062.0
8	14507.0
9	16707.0
10	18844.0
11	20974.0
12	23155.0
13	25080.0
14	26896.0
15	28461.0
16	29775.0
17	31154.0
18	30217.0
19	29668.0
20	29586.0
21	30626.0
22	33108.0
23	37854.0
24	43867.0
25	50880.0
26	60280.0
27	71080.0
28	83604.0
29	98457.0
30	115796.0
31	137110.0
32	163105.0
33	200150.0
34	248011.0
35	319388.0
36	417109.0
37	473420.0
38	259757.0
39	16000.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.877684373907734	19.446389535607018	13.753117576317674	24.92280851416757
2	16.076409387868164	20.746545356396712	37.98119860309506	25.195846652640068
3	19.22726790259571	24.45872297975923	29.255997443109333	27.058011674535727
4	13.915531503530485	16.646339137692916	36.057163081186225	33.38096627759037
5	14.616149428974436	36.7943926587538	34.206691475312226	14.38276643695954
6	33.51753523590135	35.349393711073006	17.09493880290564	14.038132250120002
7	29.14125667271375	31.477713017653386	20.97156370043719	18.409466609195675
8	26.563674222635942	34.49426013707003	20.06529399315982	18.876771647134213
9	26.559939276816248	15.775979054764544	19.48686659845966	38.177215069959544
10-11	25.557682965249036	25.316865909707364	27.780853223972734	21.344597901070863
12-13	26.24009165267519	22.56929369017738	27.516652889761378	23.673961767386054
14-15	23.363391617410137	24.298953674569365	24.948351120337858	27.38930358768264
16-17	23.669359258098442	27.218133925128573	25.661446994385507	23.451059822387474
18-19	23.59469301431893	26.50633403110707	26.959628086746427	22.93934486782757
20-21	24.304226696668053	25.772346627483678	26.696268808225703	23.227157867622562
22-23	23.956560427294857	25.620161145370346	26.555730365169637	23.86754806216516
24-25	23.78728294291034	25.780281506832093	26.606788168844943	23.825647381412622
26-27	23.669080943757127	26.026210742606153	26.51870492828461	23.78600338535211
28-29	23.7569934115723	26.09678356671199	26.45361298846674	23.69261003324897
30-31	23.973016820427993	25.904304809509583	26.31730843582158	23.805369934240844
32-33	23.75065520359181	25.93163112241088	26.2430977212592	24.074615952738103
34-35	24.084875899364068	25.958249814531342	26.30096396393466	23.65591032216993
36-37	24.134642182058503	25.704417918877304	26.23841518812544	23.922524710938752
38-39	23.958467947389106	25.81197966276721	26.332860280442787	23.8966921094009
40-41	24.09931368113525	25.6432649971465	26.39926548209391	23.85815583962434
42-43	24.000476612925997	25.59052178914934	26.31077794198716	24.098223655937502
44-45	23.843381380048488	25.682669309484886	26.378035471939253	24.095913838527373
46-47	23.93209823148142	25.605156287774445	26.250889952025762	24.211855528718374
48-49	23.757096456221497	25.64310849174029	26.27619428309901	24.323600768939208
50-51	23.69724116091991	25.8124289829636	26.326870998615952	24.163458857500537
52-53	23.934760068791245	25.572913017268473	26.31488463688728	24.177442277053
54-55	23.569640433219828	25.55226480681708	26.432784017782463	24.445310742180627
56-57	23.526308375040042	25.654470229341193	26.342107990082336	24.477113405536432
58-59	23.473357622099535	25.53931689212391	26.41662748209791	24.570698003678643
60-61	23.45465800729985	25.58806226831273	26.528405988618353	24.428873735769063
62-63	23.362180678339833	25.589357142075762	26.45582707795051	24.592635101633896
64-65	23.257643196949374	25.44488552019624	26.374569202164032	24.922902080690353
66-67	23.187591643692283	25.58708154432819	26.2366685968896	24.988658215089927
68-69	23.333322484157488	25.449681078613228	26.264064600332638	24.952931836896646
70-71	23.156874448074703	25.26636193762019	26.26727517854917	25.309488435755934
72-73	23.181305516125565	25.310022260055952	26.16068971422158	25.347982509596907
74-75	23.100977904176574	25.388745832197905	26.086158296148948	25.424117967476572
76-77	23.061564400180977	25.24920060042588	26.289349968886512	25.39988503050663
78-79	23.067820783059677	25.328469172485647	26.115623574779523	25.488086469675153
80-81	22.97609457441057	25.350241783364645	26.097834261491492	25.57582938073329
82-83	23.029088213719415	25.347574040669627	26.094877076626094	25.52846066898486
84-85	22.985723417604376	25.23405008955757	26.01549827892171	25.764728213916342
86-87	22.899676860821902	25.22582836836503	26.04914999959982	25.825344771213253
88-89	22.830209671383344	25.172048813254378	25.956345681421585	26.041395833940694
90-91	22.722121869799796	25.162508677503936	25.930441839826468	26.184927612869796
92-93	22.76720155928764	25.08325990797355	25.95975103611341	26.1897874966254
94-95	22.563129935756272	25.027294198198007	26.02385393191661	26.385721934129116
96-97	22.680980183092903	24.864607897642284	25.992785493410846	26.461626425853968
98-99	22.524155455219375	24.83490477137221	25.90498053594697	26.735959237461447
100-101	22.629590599412648	24.700843570060275	25.946058050190846	26.72350778033623
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	45.0
1	33.0
2	47.5
3	73.5
4	70.0
5	83.5
6	146.0
7	299.0
8	440.0
9	451.5
10	398.0
11	375.5
12	370.0
13	407.5
14	457.5
15	493.5
16	550.5
17	633.5
18	753.5
19	845.0
20	961.5
21	1119.5
22	1348.0
23	1677.0
24	2109.5
25	2653.0
26	3346.5
27	4477.0
28	6095.5
29	8398.5
30	11884.5
31	17066.0
32	23947.0
33	31031.5
34	38199.0
35	47024.0
36	57865.0
37	68317.0
38	77634.0
39	86258.0
40	94729.0
41	103031.5
42	110830.0
43	119330.0
44	127380.0
45	134637.0
46	141057.5
47	146053.5
48	150496.5
49	153302.5
50	151909.5
51	148243.5
52	144843.0
53	141922.0
54	137354.5
55	129119.0
56	118712.0
57	107374.0
58	97369.0
59	86027.0
60	72303.5
61	60098.0
62	49347.0
63	38356.0
64	28552.0
65	21896.0
66	16808.0
67	12934.0
68	10105.0
69	7661.0
70	5817.5
71	4483.0
72	3396.5
73	2682.5
74	2068.0
75	1515.5
76	1066.5
77	651.0
78	368.0
79	192.0
80	103.0
81	58.0
82	35.0
83	18.5
84	9.0
85	5.5
86	2.0
87	1.0
88	1.5
89	1.5
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19514453437142382
2	0.0
3	0.01147039529406339
4	0.015017940230371656
5	0.005853449144908637
6	0.10462301274662458
7	0.13850206688836852
8	0.11990701884721935
9	0.13802906089686073
10-11	0.10960435709469077
12-13	0.11168853974477189
14-15	0.11223545292245274
16-17	0.06422534478441422
18-19	0.0764348119402085
20-21	0.036111051164171216
22-23	0.08140137485104006
24-25	0.05575558124897823
26-27	0.0642992519705873
28-29	0.03883083561534089
30-31	0.023960709757315405
32-33	0.030701045136301112
34-35	0.01915674265606463
36-37	0.022719069029607516
38-39	0.030745389448004968
40-41	0.023975491194550023
42-43	0.012460751588782782
44-45	0.02594142234675419
46-47	0.03306607509383996
48-49	0.008839299466301426
50-51	0.015077065979310128
52-53	0.006326455136416407
54-55	0.012179904281325043
56-57	0.005070032971473896
58-59	0.003680577871419825
60-61	0.022837320527484457
62-63	0.009874000072724671
64-65	0.006917712625801117
66-67	0.03899343142492168
68-69	0.08715135393530637
70-71	0.020974859435922616
72-73	0.042940075166564624
74-75	0.08401768924156741
76-77	0.2566796575791376
78-79	0.0786815903998704
80-81	0.016688242637883464
82-83	0.011588646791940334
84-85	0.012978101891994404
86-87	0.2705594271424437
88-89	0.6230375794391391
90-91	0.2647355408720043
92-93	0.35034962533491043
94-95	0.0010051377319540084
96-97	0.003857955118235238
98-99	0.0030597575075658784
100-101	0.004478775482089185
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3382621.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.845308356367674
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.04312726765482	42.090044011996135
2	13.333948038216231	15.15944775680371
3	5.230620354731279	8.920086808793785
4	2.6195490510283683	5.956362942413517
5	1.403284528226697	3.988507085938326
6	0.877078661766619	2.991468418854826
7	0.5779057592178711	2.2995861758562457
8	0.3743556758877523	1.702429106463657
9	0.28452697621096895	1.4556621328575678
>10	1.1881015604018097	11.322373803805922
>50	0.04882701521825274	1.8876164501128483
>100	0.017526011027497235	1.6534019669411788
>500	7.83477554196624E-4	0.31161070522962336
>1k	3.6562285770264043E-4	0.26140263393270113
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	8.868862340770663E-5	0.0	0.0	0.0
6	0.0	8.868862340770663E-5	0.0	0.0	0.0
7	0.0	8.868862340770663E-5	0.0	0.0	0.0
8	0.0	8.868862340770663E-5	0.0	0.0	0.0
9	0.0	8.868862340770663E-5	0.0	2.956287446923554E-5	0.0
10-11	2.956287446923554E-5	8.868862340770663E-5	0.0	7.390718617308886E-5	0.0
12-13	2.956287446923554E-5	1.478143723461777E-4	0.0	1.7737724681541326E-4	0.0
14-15	2.956287446923554E-5	1.7737724681541326E-4	0.0	2.2172155851926656E-4	0.0
16-17	2.956287446923554E-5	2.069401212846488E-4	0.0	2.6606587022311986E-4	0.0
18-19	2.956287446923554E-5	2.6606587022311986E-4	0.0	2.6606587022311986E-4	0.0
20-21	4.4344311703853315E-5	3.547544936308265E-4	0.0	2.8084730745773763E-4	0.0
22-23	5.912574893847108E-5	3.547544936308265E-4	0.0	3.547544936308265E-4	0.0
24-25	5.912574893847108E-5	3.547544936308265E-4	0.0	6.060389266193286E-4	0.0
26-27	5.912574893847108E-5	3.547544936308265E-4	0.0	0.0015963952213387193	0.0
28-29	5.912574893847108E-5	3.547544936308265E-4	0.0	0.0058830120193778725	0.0
30-31	5.912574893847108E-5	3.547544936308265E-4	0.0	0.02539450916907333	0.0
32-33	5.912574893847108E-5	3.547544936308265E-4	0.0	0.07123174603362303	0.0
34-35	5.912574893847108E-5	3.990988053346798E-4	0.0	0.13102265964765192	0.0
36-37	5.912574893847108E-5	5.025688659770042E-4	0.0	0.23050173223662954	0.0
38-39	5.912574893847108E-5	5.764760521500931E-4	0.0	0.3890030837034359	0.0
40-41	5.912574893847108E-5	5.912574893847108E-4	0.0	0.5761360790936969	2.956287446923554E-5
42-43	5.912574893847108E-5	5.912574893847108E-4	0.0	0.7831648890017534	2.956287446923554E-5
44-45	5.912574893847108E-5	5.912574893847108E-4	0.0	1.0298522950102893	2.956287446923554E-5
46-47	5.912574893847108E-5	5.912574893847108E-4	0.0	1.3239733331047137	2.956287446923554E-5
48-49	5.912574893847108E-5	5.912574893847108E-4	0.0	1.636940703673276	2.956287446923554E-5
50-51	5.912574893847108E-5	5.912574893847108E-4	0.0	1.9676014546116756	2.956287446923554E-5
52-53	5.912574893847108E-5	6.060389266193286E-4	0.0	2.306140711596126	2.956287446923554E-5
54-55	5.912574893847108E-5	7.09508987261653E-4	0.0	2.6907832713153494	2.956287446923554E-5
56-57	5.912574893847108E-5	7.981976106693596E-4	0.0	3.138054780597649	2.956287446923554E-5
58-59	5.912574893847108E-5	9.164491085463018E-4	0.0	3.6178897961078107	2.956287446923554E-5
60-61	5.912574893847108E-5	9.607934202501551E-4	0.0	4.1195126501018	2.956287446923554E-5
62-63	5.912574893847108E-5	9.755748574847729E-4	0.0	4.636330821572976	2.956287446923554E-5
64-65	5.912574893847108E-5	0.0010051377319540084	0.0	5.189792175948769	2.956287446923554E-5
66-67	5.912574893847108E-5	0.0010642634808924795	0.0	5.826872120760795	2.956287446923554E-5
68-69	5.912574893847108E-5	0.0010642634808924795	0.0	6.513100344377925	2.956287446923554E-5
70-71	5.912574893847108E-5	0.0010938263553617149	0.0	7.2255508376492665	2.956287446923554E-5
72-73	5.912574893847108E-5	0.0011233892298309505	0.0	7.960203049647005	2.956287446923554E-5
74-75	5.912574893847108E-5	0.0011233892298309505	0.0	8.727610926556656	2.956287446923554E-5
76-77	5.912574893847108E-5	0.0011233892298309505	0.0	9.566368800997806	2.956287446923554E-5
78-79	5.912574893847108E-5	0.0011233892298309505	0.0	10.470475409453202	4.4344311703853315E-5
80-81	5.912574893847108E-5	0.0011529521043001862	0.0	11.397197616877563	5.912574893847108E-5
82-83	5.912574893847108E-5	0.0011529521043001862	0.0	12.349343896345466	5.912574893847108E-5
84-85	5.912574893847108E-5	0.0011529521043001862	0.0	13.313197074103186	5.912574893847108E-5
86-87	5.912574893847108E-5	0.0011825149787694216	0.0	14.3241439108904	5.912574893847108E-5
88-89	5.912574893847108E-5	0.0011825149787694216	0.0	15.395177290036337	5.912574893847108E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2935	0.0	42.298637	1
GTATCAA	5010	0.0	41.014744	1
ATCAACG	6355	0.0	31.744797	3
TCAACGC	6325	0.0	31.67022	4
CAACGCA	6380	0.0	31.393019	5
TATCAAC	6635	0.0	30.330915	2
AACGCAG	6825	0.0	29.48523	6
GTACATG	9555	0.0	24.990046	1
ACGCAGA	8020	0.0	24.795595	7
TACATGG	9790	0.0	23.853	2
CGCAGAG	8475	0.0	23.351345	8
ACATGGG	10015	0.0	21.992075	3
TAAGGCG	765	0.0	19.853168	5
GCAGAGT	9865	0.0	19.098646	9
CATGGGG	7445	0.0	18.55357	4
GAGTACA	7650	0.0	18.528866	1
AGAGTAC	8165	0.0	17.870775	10-11
AGTACAT	7440	0.0	17.416075	2
GTGTAAG	1590	0.0	17.351013	1
CAGAGTA	8835	0.0	16.596115	10-11
>>END_MODULE