FastQCFastQC Report
Mon 15 Aug 2016
SRR2920851_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920851_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2202022
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36700.045.1389731
GGTATCA19400.041.5098841
ATCAACG47950.034.7153243
TCAACGC48000.034.5836264
CAACGCA48950.033.818765
TATCAAC52000.031.8373322
AACGCAG52850.031.407066
GTACATG83200.028.096721
ACGCAGA61300.026.5510867
TACATGG84800.026.3910562
CGCAGAG64950.024.423148
ACATGGG88750.024.3363673
GCAGAGT68850.021.9076869
CATGGGG60550.021.3568974
GAGTACA58850.020.6429941
AGAGTAC59150.018.75750210-11
AGTACAT59650.018.6822262
CCCTATA3108.636562E-917.7522052
TACACCG3401.4260877E-917.533465
CAGAGTA63850.017.0536410-11
Produced by FastQC (version 0.11.5)