##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2920851_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2202022 Sequences flagged as poor quality 0 Sequence length 98 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.69878956704338 31.0 30.0 33.0 22.0 34.0 2 28.742023013394054 31.0 29.0 34.0 20.0 34.0 3 28.81094194335933 31.0 28.0 34.0 22.0 34.0 4 32.29567597417283 35.0 33.0 37.0 23.0 37.0 5 32.252785394514675 35.0 33.0 37.0 23.0 37.0 6 32.37218792546123 35.0 33.0 37.0 24.0 37.0 7 32.20078409752491 35.0 32.0 37.0 23.0 37.0 8 32.13470301386635 35.0 32.0 37.0 22.0 37.0 9 33.40753180485935 37.0 33.0 39.0 22.0 39.0 10-11 33.22179478679141 37.0 33.0 39.0 20.0 39.0 12-13 33.04692255572378 37.0 32.0 39.0 18.5 39.0 14-15 33.82081014631098 38.0 33.0 40.0 18.0 41.0 16-17 33.715420645207 38.0 32.5 40.0 17.0 41.0 18-19 33.58084047298347 38.0 32.0 40.0 17.5 41.0 20-21 33.47746253216362 38.0 32.0 40.0 17.5 41.0 22-23 33.247450979145526 38.0 32.0 40.0 16.0 41.0 24-25 33.045099685652545 38.0 32.0 40.0 14.5 41.0 26-27 33.07583961468141 38.0 32.0 40.0 12.0 41.0 28-29 32.912983158206416 38.0 32.0 40.0 10.0 41.0 30-31 32.65922706494304 38.0 31.0 40.0 9.0 41.0 32-33 32.41078177238919 37.5 31.0 40.0 8.5 41.0 34-35 32.08257864816973 37.0 30.5 40.0 7.0 41.0 36-37 31.769620149117493 37.0 30.0 40.0 2.0 41.0 38-39 31.548647334131992 37.0 30.0 40.0 2.0 41.0 40-41 31.33617715899296 37.0 30.0 40.0 2.0 41.0 42-43 31.08132162167317 36.0 30.0 40.0 2.0 41.0 44-45 30.79238195622024 36.0 29.0 39.0 2.0 41.0 46-47 30.51666740840918 36.0 28.0 39.0 2.0 40.0 48-49 30.207215913374164 35.0 28.0 39.0 2.0 40.0 50-51 29.045422797774044 34.0 25.5 38.0 2.0 39.5 52-53 29.49405591769746 34.5 27.5 38.5 2.0 39.5 54-55 30.130215320282904 35.0 28.0 39.0 2.0 40.0 56-57 30.061582718065488 35.0 28.0 39.0 2.0 40.0 58-59 29.805149085703956 35.0 28.0 39.0 2.0 40.0 60-61 29.459959301042407 35.0 27.5 39.0 2.0 40.0 62-63 29.05913633015474 34.5 26.5 38.0 2.0 40.0 64-65 28.63306996932819 34.0 26.0 38.0 2.0 40.0 66-67 28.241594770624452 34.0 26.0 37.0 2.0 40.0 68-69 27.79085858361088 34.0 25.0 37.0 2.0 39.0 70-71 27.465535539608595 33.5 25.0 36.0 2.0 39.0 72-73 27.011374772822435 33.0 23.5 36.0 2.0 39.0 74-75 26.58077121845286 33.0 22.5 35.0 2.0 38.0 76-77 26.183690489922444 33.0 21.5 35.0 2.0 37.0 78-79 25.741950579966957 32.0 20.0 35.0 2.0 37.0 80-81 25.31344827617526 32.0 18.5 35.0 2.0 36.0 82-83 24.85522124665421 32.0 17.0 35.0 2.0 36.0 84-85 24.464412253828527 31.0 10.0 34.5 2.0 35.0 86-87 24.021692562562954 31.0 6.0 34.0 2.0 35.0 88-89 23.500854896090956 31.0 2.0 34.0 2.0 35.0 90-91 23.012573216798017 31.0 2.0 34.0 2.0 35.0 92-93 22.48169931998863 30.0 2.0 34.0 2.0 35.0 94-95 21.844247923045273 30.0 2.0 34.0 2.0 35.0 96-97 21.20527814890133 29.0 2.0 34.0 2.0 35.0 98 20.810766649924478 29.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 111457.0 3 19129.0 4 14243.0 5 11759.0 6 11104.0 7 11292.0 8 12812.0 9 14570.0 10 16089.0 11 16914.0 12 17513.0 13 17545.0 14 16174.0 15 16747.0 16 18168.0 17 19697.0 18 19940.0 19 19916.0 20 20804.0 21 22130.0 22 24006.0 23 26930.0 24 30950.0 25 34866.0 26 40459.0 27 47557.0 28 54760.0 29 63465.0 30 74442.0 31 88074.0 32 105150.0 33 127995.0 34 159859.0 35 203074.0 36 255230.0 37 269582.0 38 152340.0 39 15279.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.702548981086736 17.386228911000124 12.891997974343816 25.019224133569324 2 16.389832564084745 18.94154846311536 38.697133180619204 25.971485792180694 3 20.15005334715348 22.736061721907124 27.90553488640395 29.208350044535447 4 13.789773695027648 14.78101899690009 35.055310161581524 36.37389714649074 5 15.24971962667764 36.11930617311877 33.10839875377521 15.52257544642837 6 35.013720084832414 33.52878946653603 16.62076649873701 14.836723949894552 7 30.85495392013793 30.56254629900882 19.70672033377526 18.87577944707799 8 27.781489331843062 33.41193451092794 19.266901713254367 19.539674443974633 9 27.30908228891446 15.813148097521278 18.829148845924337 38.048620767639925 10-11 26.654530848132048 24.58288894999436 26.429215155256202 22.33336504661739 12-13 27.179170322573615 21.81933824905219 26.57796664359585 24.423524784778348 14-15 24.112965798401742 23.52090196068636 23.99625151881836 28.369880722093537 16-17 24.442526908578955 26.4922112720832 24.790907852309367 24.274353967028475 18-19 24.514841193909973 25.555778157564063 26.227945365289894 23.70143528323607 20-21 25.263215302197956 24.823123725337588 25.967970822657886 23.945690149806566 22-23 24.720806252630794 24.55432186647975 25.98476934923583 24.740102531653623 24-25 24.579860667742416 24.89440567035302 25.918744510117286 24.606989151787275 26-27 24.266749620979382 25.160856532061327 25.911217374232415 24.661176472726876 28-29 24.353378209367623 25.311232920158478 25.80296241804414 24.53242645242976 30-31 24.575585801548083 25.185990742036495 25.579963069921796 24.658460386493626 32-33 24.24721618687039 25.165543512104655 25.491759104637673 25.095481196387283 34-35 24.723225627725824 25.155667074306482 25.602392620929603 24.51871467703809 36-37 24.818032482348684 24.889988785497188 25.651794480200717 24.64018425195341 38-39 24.659133551031022 24.97105791724218 25.512214984642164 24.857593547084633 40-41 24.7980818258244 24.98373954990892 25.776058640281885 24.442119983984796 42-43 24.860734428364708 24.935252625352696 25.533427613598064 24.67058533268453 44-45 24.574471043787778 24.977511353745836 25.67214038568769 24.775877216778696 46-47 24.656842239641097 24.917471849471752 25.624588954985374 24.80109695590178 48-49 24.68339758570928 25.050061235975164 25.387571410629324 24.878969767686232 50-51 24.47384766170345 25.356559595648783 25.45805767817153 24.71153506447624 52-53 24.75372896045249 25.01808602837203 25.6062354604753 24.62194955070018 54-55 24.384655525693027 24.8560394988204 25.901554919380292 24.85775005610628 56-57 24.23724438960171 24.9479807217444 25.796887917892764 25.017886970761126 58-59 24.344306394619856 24.767559557733957 25.744830730650857 25.143303316995326 60-61 24.1670573128045 24.752137305581346 26.040517148605442 25.040288233008717 62-63 24.122509272197995 24.908828135507445 25.98173599254121 24.98692659975335 64-65 24.03162549343561 24.73479689200568 25.94556722173516 25.28801039282355 66-67 24.071400464217078 24.854478782314125 25.93419979583573 25.13992095763306 68-69 24.342140511003404 24.579040830708262 25.90607931680784 25.172739341480494 70-71 24.05058993023477 24.531521968503327 25.980038038843738 25.43785006241816 72-73 24.096648262746996 24.64999627455631 25.924394228045916 25.32896123465077 74-75 24.000347186310105 24.736546188764557 25.882944449734758 25.38016217519058 76-77 24.137667873760027 24.479042319952033 26.02109345526981 25.362196351018127 78-79 23.972877828349112 24.588694371880564 26.025698357875704 25.412729441894616 80-81 24.08421029607076 24.507230371410532 26.048840750463043 25.359718582055663 82-83 24.119005084575466 24.565172765521943 26.01627146169526 25.299550688207322 84-85 23.910973640088336 24.49544066507987 26.0639074695264 25.529678225305396 86-87 23.98222298764794 24.51861328551245 26.004541525939963 25.49462220089965 88-89 23.87923570583531 24.36440055089274 25.984807375563122 25.771556367708826 90-91 23.84126270909541 24.360745773432562 26.05342342962268 25.744568087849352 92-93 24.07069669866849 24.16866379038323 26.155322495135252 25.60531701581303 94-95 23.85713520798468 24.059372440278384 26.29737941468316 25.786112937053772 96-97 24.160663854278337 23.852842900808056 26.193444165585344 25.793049079328263 98 24.01262577703574 23.842887724999752 26.179941473408313 25.964545024556195 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 23.0 1 23.5 2 39.5 3 59.5 4 73.5 5 97.0 6 163.0 7 316.5 8 483.5 9 558.0 10 574.5 11 605.0 12 705.0 13 802.0 14 919.5 15 1028.5 16 1107.0 17 1157.5 18 1175.0 19 1240.0 20 1270.5 21 1281.5 22 1381.0 23 1562.0 24 1813.5 25 2206.0 26 2825.5 27 3639.5 28 4827.5 29 6716.5 30 9575.0 31 14000.0 32 17841.5 33 21018.0 34 25032.0 35 29558.5 36 34253.0 37 38626.0 38 43591.5 39 48692.0 40 53239.0 41 58476.5 42 65092.5 43 71194.0 44 76508.5 45 82287.5 46 87932.0 47 92716.0 48 96726.0 49 99031.0 50 99463.0 51 99436.0 52 99384.0 53 100109.0 54 98747.0 55 95108.5 56 89182.5 57 81293.5 58 75956.0 59 68647.0 60 58959.5 61 50123.5 62 41736.5 63 32796.0 64 24400.5 65 18687.5 66 14475.0 67 11341.5 68 8719.0 69 6645.5 70 5067.5 71 3886.0 72 3246.5 73 2772.0 74 2266.0 75 1751.5 76 1218.0 77 804.5 78 480.0 79 269.5 80 170.0 81 111.0 82 64.0 83 32.5 84 17.0 85 12.5 86 9.0 87 5.5 88 8.0 89 7.0 90 3.5 91 5.0 92 4.5 93 1.0 94 2.0 95 2.0 96 2.0 97 2.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3707955687999484 2 0.023750898038257563 3 0.018392186817388745 4 0.01575824401391085 5 0.022388513829562102 6 0.022933467513040286 7 0.013669254893911142 8 0.008583020514781416 9 0.0 10-11 2.270640347825771E-5 12-13 0.004813757537390635 14-15 0.0028837132417387293 16-17 0.005540362448694881 18-19 0.035603640653908086 20-21 0.07649787331825023 22-23 0.09609349951998664 24-25 0.06280591202086083 26-27 0.07329627042781589 28-29 0.05533550527651404 30-31 0.05022656449390606 32-33 0.01864195725564958 34-35 0.3303327578016932 36-37 0.5044908724799297 38-39 0.4606902201703707 40-41 0.47022690963123887 42-43 0.4209313076799414 44-45 0.39477353087298855 46-47 0.39416045797907556 48-49 0.3841242276416857 50-51 0.3807636799269036 52-53 0.4415033092312429 54-55 0.44295651905385147 56-57 0.4125753511999426 58-59 0.3983611426225533 60-61 0.4120758103234209 62-63 0.3922304136834237 64-65 0.4423207397564602 66-67 0.46137141227471845 68-69 0.40442375235124806 70-71 0.3609864024973411 72-73 0.34752150523473424 74-75 0.3285616583303891 76-77 0.3277896406121283 78-79 0.3275398701738675 80-81 0.3434797654156044 82-83 0.3380302285808225 84-85 0.33871142068517024 86-87 0.32538276184343295 88-89 0.3691380013460356 90-91 0.3464543042712561 92-93 0.336395367530388 94-95 0.34375224225734347 96-97 0.3435251782225609 98 0.3389611911234311 >>END_MODULE >>Sequence Length Distribution pass #Length Count 98 2202022.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.44624805522604 #Duplication Level Percentage of deduplicated Percentage of total 1 73.23115902548352 38.40699531622214 2 12.318723642376609 12.921416717437225 3 5.495081389931483 8.645892049800088 4 2.869358967375763 6.019484486499061 5 1.6917361820131502 4.436260772293136 6 1.0810930878616805 3.401956575407038 7 0.7094029963726733 2.604386786321729 8 0.5331043084179949 2.2367456638879912 9 0.3822715137623983 1.804383597170655 >10 1.5968200862934316 13.633637625518007 >50 0.05958446208338555 2.138821982926362 >100 0.029572651076177656 2.9033085170650024 >500 0.0017430724598598364 0.6178916300997204 >1k 3.4861449197188117E-4 0.22881827935187266 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3623842086954625E-4 2 0.0 0.0 0.0 0.0 1.3623842086954625E-4 3 0.0 0.0 0.0 0.0 1.3623842086954625E-4 4 0.0 0.0 0.0 0.0 1.3623842086954625E-4 5 0.0 0.0 0.0 0.0 1.3623842086954625E-4 6 0.0 0.0 0.0 0.0 1.3623842086954625E-4 7 0.0 0.0 0.0 0.0 1.3623842086954625E-4 8 0.0 0.0 0.0 0.0 1.3623842086954625E-4 9 0.0 0.0 0.0 0.0 1.3623842086954625E-4 10-11 0.0 0.0 0.0 0.0 1.3623842086954625E-4 12-13 0.0 4.541280695651542E-5 0.0 0.0 3.6330245565212337E-4 14-15 0.0 1.3623842086954625E-4 0.0 0.0 4.0871526260863875E-4 16-17 0.0 1.8165122782606169E-4 0.0 0.0 4.541280695651542E-4 18-19 0.0 1.8165122782606169E-4 0.0 2.270640347825771E-5 5.222472799999273E-4 20-21 0.0 1.8165122782606169E-4 0.0 9.082561391303084E-5 6.130728939129582E-4 22-23 0.0 1.8165122782606169E-4 0.0 2.270640347825771E-4 6.811921043477314E-4 24-25 0.0 1.8165122782606169E-4 0.0 5.222472799999273E-4 6.811921043477314E-4 26-27 0.0 1.8165122782606169E-4 0.0 0.0012715585947824317 8.174305252172775E-4 28-29 0.0 1.8165122782606169E-4 0.0 0.005177059993042758 8.62843332173793E-4 30-31 0.0 1.8165122782606169E-4 0.0 0.0190052597113017 8.855497356520507E-4 32-33 0.0 1.8165122782606169E-4 0.0 0.05009032607303651 9.536689460868239E-4 34-35 0.0 1.8165122782606169E-4 0.0 0.08975841294955272 9.990817530433392E-4 36-37 0.0 2.270640347825771E-4 0.0 0.1583998706643258 0.001021788156521597 38-39 0.0 2.951832452173502E-4 0.0 0.2764050495408311 0.0011353201739128856 40-41 0.0 3.1788964869560794E-4 0.0 0.41234828716516003 0.0011353201739128856 42-43 0.0 4.541280695651542E-4 0.0 0.5538545936416621 0.0011580265773911433 44-45 0.0 4.541280695651542E-4 0.0 0.731918209718159 0.001248852191304174 46-47 0.0 4.995408765216696E-4 0.0 0.9404084064555214 0.0013169714017389471 48-49 0.0 4.995408765216696E-4 0.0 1.1769183050850538 0.0013623842086954627 50-51 0.0 4.995408765216696E-4 0.0 1.4384052475406695 0.0013623842086954627 52-53 0.0 4.995408765216696E-4 0.0 1.7022763623615023 0.0014986226295650089 54-55 0.0 4.995408765216696E-4 0.0 1.994053647057114 0.0015440354365215242 56-57 0.0 4.995408765216696E-4 0.0 2.3318341051996754 0.0015440354365215242 58-59 0.0 4.995408765216696E-4 0.0 2.7053998552239715 0.001566741839999782 60-61 0.0 5.903664904347005E-4 0.0 3.112434843975219 0.0016348610504345552 62-63 0.0 5.903664904347005E-4 0.0 3.53116363051777 0.0016348610504345552 64-65 0.0 5.903664904347005E-4 0.0 3.9664226787924917 0.001725686664347586 66-67 0.0 6.811921043477314E-4 0.0 4.477339463456769 0.0017483930678258437 68-69 0.0 6.811921043477314E-4 0.0 5.039981435244516 0.0017710994713041014 70-71 0.0 6.811921043477314E-4 0.0 5.64111076092791 0.001793805874782359 72-73 0.0 6.811921043477314E-4 0.0 6.276118040600865 0.0019073378921736477 74-75 0.0 6.811921043477314E-4 0.0 6.907810185365996 0.0019981635060866785 76-77 0.0 6.811921043477314E-4 0.0 7.588139446381553 0.0019981635060866785 78-79 0.0 6.811921043477314E-4 0.0 8.341220024141448 0.002020869909564936 80-81 0.0 6.811921043477314E-4 0.0 9.14216570043351 0.002043576313043194 82-83 0.0 6.811921043477314E-4 0.0 9.965091175292526 0.0021571083304344823 84-85 0.0 6.811921043477314E-4 0.0 10.791649674708065 0.0022025211373909977 86 0.0 6.811921043477314E-4 0.0 11.429540667622758 0.002225227540869256 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3670 0.0 45.138973 1 GGTATCA 1940 0.0 41.509884 1 ATCAACG 4795 0.0 34.715324 3 TCAACGC 4800 0.0 34.583626 4 CAACGCA 4895 0.0 33.81876 5 TATCAAC 5200 0.0 31.837332 2 AACGCAG 5285 0.0 31.40706 6 GTACATG 8320 0.0 28.09672 1 ACGCAGA 6130 0.0 26.551086 7 TACATGG 8480 0.0 26.391056 2 CGCAGAG 6495 0.0 24.42314 8 ACATGGG 8875 0.0 24.336367 3 GCAGAGT 6885 0.0 21.907686 9 CATGGGG 6055 0.0 21.356897 4 GAGTACA 5885 0.0 20.642994 1 AGAGTAC 5915 0.0 18.757502 10-11 AGTACAT 5965 0.0 18.682226 2 CCCTATA 310 8.636562E-9 17.752205 2 TACACCG 340 1.4260877E-9 17.53346 5 CAGAGTA 6385 0.0 17.05364 10-11 >>END_MODULE