FastQCFastQC Report
Mon 15 Aug 2016
SRR2920851_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920851_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2202022
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39150.042.1508261
GGTATCA24300.042.0765881
ATCAACG51450.031.1915953
TCAACGC51850.030.8593944
CAACGCA52950.030.0355575
TATCAAC55650.029.2634372
AACGCAG56400.028.0305656
GTACATG84150.024.0748621
ACGCAGA66450.023.5757737
TACATGG86050.023.3391952
CGCAGAG69650.022.4900478
ACATGGG90500.021.4051283
AGTACCG3700.020.5293455
GCAGAGT75400.020.1454149
CATGGGG61500.018.9918044
GAGTACA64300.018.9337791
GAGTACT24500.017.3366712-13
GTATAGG3852.4556357E-1017.2932111
AGAGTAC66850.017.18212910-11
AGTACAT65900.017.146992
Produced by FastQC (version 0.11.5)