##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920851_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2202022
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.018497544529527	31.0	30.0	34.0	26.0	34.0
2	30.03900596815109	31.0	30.0	34.0	26.0	34.0
3	30.17199237791448	31.0	30.0	34.0	26.0	34.0
4	33.80774806064608	35.0	35.0	37.0	30.0	37.0
5	33.290873569837174	35.0	33.0	37.0	27.0	37.0
6	33.233780588931445	35.0	33.0	37.0	27.0	37.0
7	33.041045911439575	35.0	33.0	37.0	26.0	37.0
8	32.953379212378444	35.0	33.0	37.0	26.0	37.0
9	34.254256315332	37.0	34.0	39.0	27.0	39.0
10-11	34.05223290230525	37.0	33.5	39.0	25.5	39.0
12-13	33.854168577788954	37.0	33.0	39.0	25.0	39.0
14-15	34.71019544763858	38.0	33.0	40.0	25.0	41.0
16-17	34.569539041844266	38.0	33.0	40.0	24.5	41.0
18-19	34.45700951216655	38.0	33.0	40.0	24.0	41.0
20-21	34.320109426699645	38.0	33.0	40.0	22.5	41.0
22-23	34.16153744149695	38.0	33.0	40.0	22.0	41.0
24-25	33.954601725141714	38.0	33.0	40.0	19.5	41.0
26-27	33.74341764069569	38.0	32.0	40.0	19.0	41.0
28-29	33.66690455408711	38.0	32.5	40.0	18.5	41.0
30-31	33.64829870001299	38.0	32.0	40.0	18.0	41.0
32-33	33.479980445245324	38.0	32.0	40.0	18.0	41.0
34-35	33.297240218308445	38.0	32.0	40.0	16.0	41.0
36-37	33.0757951101306	38.0	31.5	40.0	15.5	41.0
38-39	32.861142168425204	37.5	31.0	40.0	15.0	41.0
40-41	32.600715842076056	37.0	31.0	40.0	12.0	41.0
42-43	32.59936980647787	37.0	31.0	40.0	10.5	41.0
44-45	32.42863173029152	37.0	31.0	40.0	9.0	41.0
46-47	32.142996527736784	37.0	30.5	40.0	8.0	41.0
48-49	31.840187791039327	37.0	30.0	40.0	6.5	41.0
50-51	31.536154270938255	36.0	30.0	40.0	2.0	41.0
52-53	31.242336134698018	36.0	29.5	39.0	2.0	41.0
54-55	30.92309568205949	35.5	29.0	39.0	2.0	40.5
56-57	30.561923995309765	35.0	28.5	39.0	2.0	40.0
58-59	30.191058944915174	35.0	28.0	39.0	2.0	40.0
60-61	29.828355257122773	35.0	28.0	38.0	2.0	40.0
62-63	29.48357895606856	34.0	27.5	38.0	2.0	40.0
64-65	29.057357056378184	34.0	26.5	37.5	2.0	40.0
66-67	28.599350733098944	34.0	26.0	37.0	2.0	39.5
68-69	28.137386002501337	33.5	26.0	36.0	2.0	39.0
70-71	27.70120416598926	33.0	25.5	36.0	2.0	39.0
72-73	27.259971744151514	33.0	24.5	35.5	2.0	38.5
74-75	26.81418214713568	32.5	24.0	35.0	2.0	37.5
76-77	25.469107029811692	30.5	21.5	34.0	2.0	36.0
78-79	26.313304090513174	32.0	23.5	35.0	2.0	36.0
80-81	26.349128437408893	33.0	24.0	35.0	2.0	36.0
82-83	26.009971744151514	32.0	23.0	35.0	2.0	36.0
84-85	25.676893782169298	32.0	21.0	35.0	2.0	35.0
86-87	25.130906957332854	32.0	19.0	34.5	2.0	35.0
88-89	24.753611907601286	31.5	17.5	34.0	2.0	35.0
90-91	24.365823320566278	31.0	14.0	34.0	2.0	35.0
92-93	24.03099946322062	31.0	7.0	34.0	2.0	35.0
94-95	23.617162998371498	31.0	2.0	34.0	2.0	35.0
96-97	23.034066190074395	31.0	2.0	34.0	2.0	35.0
98-99	22.36970089308826	31.0	2.0	34.0	2.0	35.0
100-101	20.16976079258064	27.0	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	59892.0
3	26684.0
4	14724.0
5	10503.0
6	9427.0
7	9349.0
8	10340.0
9	11971.0
10	13275.0
11	15006.0
12	16039.0
13	17559.0
14	18835.0
15	19916.0
16	21227.0
17	21841.0
18	21143.0
19	20445.0
20	20525.0
21	21116.0
22	22844.0
23	25637.0
24	29836.0
25	34835.0
26	41270.0
27	48387.0
28	57239.0
29	66918.0
30	78106.0
31	92350.0
32	109862.0
33	133511.0
34	167191.0
35	214200.0
36	272342.0
37	283535.0
38	136305.0
39	7837.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.640539207567976	18.918445492317186	13.73466030178007	24.706354998334767
2	16.681440966529852	20.185356912873715	37.238274640307864	25.894927480288572
3	20.08409551899325	23.646709801424482	28.315428139570585	27.95376654001168
4	14.471388738626418	15.90032806423028	34.59908080245156	35.029202394691744
5	15.210488118800189	36.407718488093366	33.16290337084367	15.218890022262773
6	34.950126904649444	34.124689784846275	16.32544325705113	14.599740053453155
7	30.63871470324876	30.74703849041571	19.785235131286885	18.829011675048648
8	27.690616295787056	33.82583890084688	19.1171001097589	19.366444693607157
9	27.10421518350107	15.694101671730381	18.8460189933601	38.35566415140845
10-11	26.68783435375588	24.747167526149322	26.420942371244678	22.14405574885012
12-13	27.17453865982897	21.940106475356547	26.620953186304597	24.264401678509888
14-15	24.086284071481035	23.62962217018629	23.976966181923245	28.307127576409428
16-17	24.41050829920065	26.65611955992981	24.767660325906053	24.165711814963487
18-19	24.516897097644517	25.599255749185296	26.31512450271743	23.568722650452763
20-21	25.23645340449972	24.892576933813945	25.925319361728565	23.945650299957773
22-23	24.74620434229014	24.629785462479283	26.06935274693335	24.554657448297224
24-25	24.580369074378762	25.017754883104033	25.949891925810714	24.451984116706495
26-27	24.308991320797844	25.221990038650727	25.93854282378191	24.53047581676952
28-29	24.375131606534453	25.283401255744977	25.888217753928778	24.4532493837918
30-31	24.56825811984525	25.14548108121367	25.684843303782124	24.60141749515896
32-33	24.238345433615923	25.190453390365626	25.593960185815277	24.97724099020318
34-35	24.619800618457056	25.196288349242916	25.782110166750847	24.40180086554918
36-37	24.834487172557143	24.96240048221864	25.71014371623595	24.49296862898826
38-39	24.630551046895064	25.0074614356211	25.63712937561526	24.72485814186858
40-41	24.811716716557573	25.00681362907512	25.810095072837697	24.371374581529615
42-43	24.792222567906055	25.021130870600956	25.587588083142766	24.599058478350226
44-45	24.532043826339912	24.967549966238337	25.74151308119629	24.758893126225466
46-47	24.601684684408433	24.973737139716146	25.64704305746257	24.77753511841285
48-49	24.597473388190433	25.1366355987396	25.413108660839423	24.85278235223054
50-51	24.45099497544497	25.358412581258694	25.46444488602492	24.72614755727141
52-53	24.729944351618926	25.076492112808214	25.547752560220648	24.645810975352216
54-55	24.45465749309018	24.871733207797313	25.76324787664042	24.910361422472082
56-57	24.306193259488353	25.00874240999859	25.60842632078514	25.07663800972792
58-59	24.313307678805675	24.88114514835824	25.63633923650872	25.16920793632736
60-61	24.236808306959603	24.9095457585699	25.81546221884578	25.03818371562472
62-63	24.154423720099626	25.057702474144023	25.692974695387257	25.094899110369095
64-65	23.988899501017993	24.896758819610305	25.686900711799854	25.427440967571847
66-67	24.014276099232156	24.98753499004957	25.6807144773339	25.317474433384373
68-69	24.278470132828083	24.72988153490331	25.650158095122517	25.34149023714609
70-71	23.94454066335177	24.695615915318882	25.692287846393747	25.6675555749356
72-73	23.976340640977995	24.772065818777563	25.585464187028695	25.66612935321575
74-75	23.82589815606634	24.867663063671948	25.504389338868688	25.802049441393027
76-77	23.889834995126833	24.641044865465513	25.825559253488034	25.64356088591962
78-79	23.713526657981443	24.71035048358431	25.793572070208064	25.78255078822618
80-81	23.71920490336992	24.71358666684532	25.745598964785586	25.821609464999174
82-83	23.722447307459017	24.768770053573085	25.715013703819018	25.79376893514888
84-85	23.431836132570236	24.693071042738644	25.74247262721922	26.1326201974719
86-87	23.44249748567781	24.684474760484918	25.720229810641605	26.152797943195665
88-89	23.311241877340443	24.580687655111014	25.70263875203416	26.40543171551438
90-91	23.130272609386154	24.611793889236957	25.715558659828364	26.542374841548526
92-93	23.314009007992905	24.482218134515623	25.73295609771214	26.470816759779336
94-95	22.915445711954067	24.395418800049683	25.89950633503777	26.789629152958483
96-97	23.072247120771703	24.215945471608954	25.914473879911558	26.797333527707785
98-99	22.83378690908843	24.236227248833135	25.857905331530382	27.072080510548048
100-101	23.014157695675394	24.04805459982052	25.91847448944372	27.019313215060365
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	27.0
1	18.5
2	27.5
3	47.5
4	43.5
5	46.0
6	91.5
7	191.0
8	297.0
9	340.0
10	307.0
11	284.5
12	288.5
13	284.5
14	304.5
15	371.5
16	422.0
17	456.5
18	530.5
19	584.0
20	665.5
21	822.0
22	987.5
23	1181.0
24	1485.0
25	1927.5
26	2481.0
27	3193.5
28	4209.0
29	5698.5
30	7944.5
31	11265.5
32	15624.0
33	19164.0
34	21971.5
35	25754.5
36	30184.0
37	34654.0
38	39132.5
39	43600.0
40	48286.5
41	53340.5
42	58750.5
43	65463.5
44	71384.5
45	76184.0
46	82226.0
47	87345.0
48	91659.0
49	95996.0
50	97874.5
51	98137.5
52	98314.0
53	99048.5
54	98323.0
55	94284.0
56	88201.5
57	80804.0
58	75042.0
59	68512.0
60	59175.0
61	50528.0
62	42320.0
63	33153.5
64	25137.0
65	19568.5
66	15148.0
67	11689.0
68	9000.0
69	6777.5
70	5290.0
71	4231.0
72	3355.0
73	2902.0
74	2359.5
75	1815.5
76	1320.0
77	864.0
78	564.5
79	325.5
80	173.0
81	107.5
82	67.5
83	29.0
84	15.5
85	12.0
86	10.0
87	5.0
88	3.0
89	2.5
90	0.5
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18755489273040868
2	0.0
3	0.010899073669563702
4	0.01448668541912842
5	0.006357792973912159
6	0.10622055547128957
7	0.13891777647998066
8	0.12061641527650495
9	0.1381911715686764
10-11	0.11094348739476717
12-13	0.11135220265737582
14-15	0.11232857800694089
16-17	0.06441806666781713
18-19	0.07667952454607628
20-21	0.036125887933908016
22-23	0.08072126436520616
24-25	0.055358211679992296
26-27	0.0643272410539041
28-29	0.03898689477216849
30-31	0.024205026107822717
32-33	0.03160731364173473
34-35	0.018800902079997385
36-37	0.02411420049390969
38-39	0.031925203290430346
40-41	0.02506786943999651
42-43	0.012125219457389617
44-45	0.025453878299126896
46-47	0.03267451460521284
48-49	0.008174305252172777
50-51	0.015190583926954408
52-53	0.007311461919998983
54-55	0.011966274633041812
56-57	0.005086234379129727
58-59	0.0032470156973908528
60-61	0.022456633039996877
62-63	0.009536689460868239
64-65	0.006312380166955643
66-67	0.03907772038608152
68-69	0.0873061213739009
70-71	0.020208699095649364
72-73	0.04305134099477662
74-75	0.08453594014955346
76-77	0.2406424640625752
78-79	0.07872310085911949
80-81	0.016008014452171686
82-83	0.011966274633041812
84-85	0.013215126824345986
86-87	0.2545387829912689
88-89	0.611642390493828
90-91	0.26185024491126796
92-93	0.34684031313038655
94-95	0.0010217881565215972
96-97	0.003928207801738584
98-99	0.003383254118260399
100-101	0.004995408765216696
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2202022.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.65765334009591
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.72222186212765	36.332794518731106
2	12.819226541409808	12.987838684457891
3	5.779782142457416	8.783706004616537
4	3.088713504433889	6.258679118979389
5	1.8106471857041258	4.586156872730992
6	1.199066196925266	3.644512784140038
7	0.7854460507625868	2.7852197629814945
8	0.5691268968307214	2.3064506436940166
9	0.40594772303262794	1.850792312482932
>10	1.7196984673104199	14.206203076071706
>50	0.06531398995315983	2.274852664874999
>100	0.03237648639361996	3.082811664186343
>500	0.0021626245854681567	0.7279372708746216
>1k	2.7032807318344885E-4	0.1720446211780205
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.270640347825771E-4
2	0.0	0.0	0.0	0.0	2.270640347825771E-4
3	0.0	0.0	0.0	0.0	2.270640347825771E-4
4	0.0	0.0	0.0	0.0	2.270640347825771E-4
5	0.0	0.0	0.0	0.0	2.724768417390925E-4
6	0.0	0.0	0.0	0.0	3.1788964869560794E-4
7	0.0	0.0	0.0	0.0	3.1788964869560794E-4
8	0.0	0.0	0.0	0.0	3.1788964869560794E-4
9	0.0	0.0	0.0	0.0	3.1788964869560794E-4
10-11	0.0	4.541280695651542E-5	0.0	0.0	3.1788964869560794E-4
12-13	0.0	6.811921043477313E-5	0.0	0.0	5.44953683478185E-4
14-15	0.0	9.082561391303084E-5	0.0	0.0	6.130728939129582E-4
16-17	0.0	9.082561391303084E-5	0.0	0.0	6.811921043477314E-4
18-19	0.0	9.082561391303084E-5	0.0	2.270640347825771E-5	6.811921043477314E-4
20-21	0.0	9.082561391303084E-5	0.0	9.082561391303084E-5	7.266049113042467E-4
22-23	0.0	9.082561391303084E-5	0.0	2.270640347825771E-4	7.266049113042467E-4
24-25	0.0	1.3623842086954625E-4	0.0	4.995408765216696E-4	7.720177182607621E-4
26-27	0.0	2.270640347825771E-4	0.0	0.0013169714017389471	7.720177182607621E-4
28-29	0.0	2.270640347825771E-4	0.0	0.005222472799999273	7.720177182607621E-4
30-31	0.0	2.270640347825771E-4	0.0	0.018778195676519126	7.720177182607621E-4
32-33	0.0	2.270640347825771E-4	0.0	0.04925018914434097	8.174305252172776E-4
34-35	0.0	2.951832452173502E-4	0.0	0.0884868543547703	8.174305252172776E-4
36-37	0.0	3.8600885913038106E-4	0.0	0.15601569829910872	8.174305252172776E-4
38-39	0.0	4.7683447304341193E-4	0.0	0.2707965678817015	8.401369286955353E-4
40-41	0.0	5.222472799999273E-4	0.0	0.4049914124382045	8.62843332173793E-4
42-43	0.0	5.903664904347005E-4	0.0	0.5450445090920981	8.62843332173793E-4
44-45	0.0	5.903664904347005E-4	0.0	0.7203379439442477	9.082561391303085E-4
46-47	0.0	6.584857008694737E-4	0.0	0.9255130057737844	0.001021788156521597
48-49	0.0	6.811921043477314E-4	0.0	1.1556651114294043	0.00108990736695637
50-51	0.0	7.266049113042467E-4	0.0	1.409113987053717	0.00108990736695637
52-53	0.0	7.720177182607621E-4	0.0	1.6656282271475944	0.0012261457878259164
54-55	0.0	7.720177182607621E-4	0.0	1.949730747467555	0.0012261457878259164
56-57	0.0	7.947241217390198E-4	0.0	2.279677496410118	0.0012942649982606897
58-59	0.0	8.174305252172776E-4	0.0	2.644387749077893	0.0013623842086954627
60-61	0.0	8.401369286955353E-4	0.0	3.039206692757838	0.0013623842086954627
62-63	0.0	8.62843332173793E-4	0.0	3.4453561317734334	0.0014532098226084935
64-65	0.0	8.62843332173793E-4	0.0	3.867127576382071	0.0014759162260867512
66-67	0.0	9.082561391303084E-4	0.0	4.363557675627219	0.0014986226295650089
68-69	0.0	9.082561391303084E-4	0.0	4.9097602112967085	0.0014986226295650089
70-71	0.0	9.309625426085662E-4	0.0	5.492497350162714	0.0014986226295650089
72-73	0.0	9.536689460868239E-4	0.0	6.110452120823497	0.001634861050434555
74-75	0.0	9.990817530433392E-4	0.0	6.728633955519064	0.0017710994713041014
76-77	4.541280695651542E-5	0.0010444945599998546	0.0	7.396179511376362	0.0018165122782606167
78-79	4.541280695651542E-5	0.0010444945599998546	0.0	8.132480056965825	0.0019073378921736477
80-81	4.541280695651542E-5	0.0010672009634781123	0.0	8.91319432775876	0.0019073378921736477
82-83	4.541280695651542E-5	0.00108990736695637	0.0	9.715411562645604	0.0020662827165214516
84-85	4.541280695651542E-5	0.00108990736695637	0.0	10.523691407261143	0.0020889891199997093
86-87	6.811921043477313E-5	0.00108990736695637	0.0	11.357288891754942	0.0022933467513040285
88-89	9.082561391303084E-5	0.00108990736695637	0.0	12.232370975403516	0.002497704382608348
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3915	0.0	42.150826	1
GGTATCA	2430	0.0	42.076588	1
ATCAACG	5145	0.0	31.191595	3
TCAACGC	5185	0.0	30.859394	4
CAACGCA	5295	0.0	30.035557	5
TATCAAC	5565	0.0	29.263437	2
AACGCAG	5640	0.0	28.030565	6
GTACATG	8415	0.0	24.074862	1
ACGCAGA	6645	0.0	23.575773	7
TACATGG	8605	0.0	23.339195	2
CGCAGAG	6965	0.0	22.490047	8
ACATGGG	9050	0.0	21.405128	3
AGTACCG	370	0.0	20.529345	5
GCAGAGT	7540	0.0	20.145414	9
CATGGGG	6150	0.0	18.991804	4
GAGTACA	6430	0.0	18.933779	1
GAGTACT	2450	0.0	17.33667	12-13
GTATAGG	385	2.4556357E-10	17.293211	1
AGAGTAC	6685	0.0	17.182129	10-11
AGTACAT	6590	0.0	17.14699	2
>>END_MODULE