FastQCFastQC Report
Mon 15 Aug 2016
SRR2920850_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920850_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3999666
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58400.045.868111
GGTATCA30100.042.967541
TCAACGC75950.034.775084
CAACGCA77450.033.805565
TATCAAC79050.033.413442
ATCAACG79000.033.374463
AACGCAG81400.032.0468446
ACGCAGA98550.026.235057
GTACATG126500.024.9957921
CGCAGAG104400.024.4996858
TACATGG129850.023.731652
ACATGGG133900.022.7384553
GCAGAGT114150.021.2026549
CATGGGG95500.020.1659834
AGAGTAC98000.018.63962710-11
TAAGGCG12800.018.2697945
GAGTACA97300.017.9278031
GTATTAA23400.017.3088231
AGTACAT96250.017.2467982
TATTAAG19850.016.4020632
Produced by FastQC (version 0.11.5)