##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920850_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3999666
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.674550324952133	31.0	30.0	33.0	22.0	34.0
2	28.703035203439487	31.0	28.0	34.0	20.0	34.0
3	28.775487003164763	31.0	28.0	34.0	22.0	34.0
4	32.26424756467165	35.0	32.0	37.0	23.0	37.0
5	32.2365162491068	35.0	32.0	37.0	23.0	37.0
6	32.359534271111634	35.0	33.0	37.0	24.0	37.0
7	32.1953065580976	35.0	32.0	37.0	23.0	37.0
8	32.136660911186084	35.0	32.0	37.0	22.0	37.0
9	33.406195667338224	37.0	33.0	39.0	22.0	39.0
10-11	33.21550686982363	37.0	32.5	39.0	20.5	39.0
12-13	33.04392241752187	37.0	32.0	39.0	18.5	39.0
14-15	33.807132645575905	38.0	32.5	40.0	18.0	41.0
16-17	33.69989931659293	38.0	32.0	40.0	17.5	41.0
18-19	33.5841115233122	38.0	32.0	40.0	17.5	41.0
20-21	33.49235286146393	38.0	32.0	40.0	18.0	41.0
22-23	33.271948707717094	38.0	32.0	40.0	16.5	41.0
24-25	33.07856093483806	38.0	32.0	40.0	15.5	41.0
26-27	33.11106777415914	38.0	32.0	40.0	14.0	41.0
28-29	32.955805059722486	38.0	32.0	40.0	10.0	41.0
30-31	32.70856153988858	38.0	31.0	40.0	9.5	41.0
32-33	32.465935405606366	37.5	31.0	40.0	9.0	41.0
34-35	32.1422381268836	37.0	30.5	40.0	8.0	41.0
36-37	31.833967261266316	37.0	30.0	40.0	6.5	41.0
38-39	31.63641789089389	37.0	30.0	40.0	2.0	41.0
40-41	31.44397194665755	37.0	30.0	40.0	2.0	41.0
42-43	31.207379941225092	36.5	30.0	40.0	2.0	41.0
44-45	30.931904439020656	36.0	29.0	39.5	2.0	41.0
46-47	30.681695671588578	36.0	28.5	39.0	2.0	40.5
48-49	30.38475025164601	36.0	28.0	39.0	2.0	40.0
50-51	29.241490664470483	34.0	26.5	38.0	2.0	39.5
52-53	29.684656418810974	34.5	27.5	38.5	2.0	39.5
54-55	30.33860239830026	36.0	28.0	39.0	2.0	40.0
56-57	30.285153435311848	35.5	28.0	39.0	2.0	41.0
58-59	30.045989715141214	35.0	28.0	39.0	2.0	41.0
60-61	29.717983326607772	35.0	28.0	39.0	2.0	40.0
62-63	29.32735720932698	35.0	27.0	39.0	2.0	40.0
64-65	28.905923394603448	34.0	26.0	38.0	2.0	40.0
66-67	28.521850074481222	34.0	26.0	38.0	2.0	40.0
68-69	28.073527389537027	34.0	26.0	37.0	2.0	39.5
70-71	27.74206746263313	34.0	25.5	36.5	2.0	39.0
72-73	27.281490504457125	33.0	24.5	36.0	2.0	39.0
74-75	26.845277830698862	33.0	23.5	35.5	2.0	38.5
76-77	26.42485522541132	33.0	22.5	35.0	2.0	37.5
78-79	25.9620944598874	32.0	20.5	35.0	2.0	37.0
80-81	25.50999695974614	32.0	19.5	35.0	2.0	36.5
82-83	25.034813656940354	32.0	18.0	35.0	2.0	36.0
84-85	24.63491851569606	31.5	13.0	35.0	2.0	35.5
86-87	24.17853628277961	31.0	7.0	34.0	2.0	35.0
88-89	23.639287005464958	31.0	2.0	34.0	2.0	35.0
90-91	23.14833701114043	31.0	2.0	34.0	2.0	35.0
92-93	22.610670991027753	30.5	2.0	34.0	2.0	35.0
94-95	21.972010287859035	30.0	2.0	34.0	2.0	35.0
96-97	21.326563018012003	29.5	2.0	34.0	2.0	35.0
98	20.930378436599455	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	195774.0
3	34871.0
4	25790.0
5	20947.0
6	19190.0
7	19956.0
8	22771.0
9	26004.0
10	28437.0
11	30360.0
12	31798.0
13	31455.0
14	29721.0
15	31005.0
16	32847.0
17	35350.0
18	36778.0
19	36459.0
20	38219.0
21	40129.0
22	43758.0
23	49080.0
24	55867.0
25	63978.0
26	74073.0
27	84966.0
28	98470.0
29	115195.0
30	133483.0
31	156886.0
32	187357.0
33	226463.0
34	282357.0
35	357746.0
36	456253.0
37	506930.0
38	307593.0
39	31346.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.94836694912382	18.023946308455262	13.302690225543149	24.724996516877766
2	15.941257337268382	19.52076642959161	39.236101298631795	25.301874934508213
3	19.138460799786337	23.639037591623378	29.039299617414336	28.18320199117595
4	13.342995828672755	15.681399721478071	36.46584230377495	34.50976214607422
5	14.59405861360982	36.71533211794745	34.06776651586878	14.622842752573941
6	33.572449424883494	34.850072084936436	17.345648833199334	14.231829656980736
7	29.410782123016787	31.403898512251864	20.878485565622356	18.30683379910899
8	26.917905754711203	34.04582614968575	20.030105682447562	19.006162413155483
9	26.91169712671008	15.793993798482173	19.444323601020685	37.849985473787065
10-11	25.704215228025344	25.201110593012167	27.596936243410386	21.497737935552102
12-13	26.346254196515574	22.542117676709967	27.40520006630913	23.706428060465328
14-15	23.511669949765277	24.11472913842633	24.85721740171863	27.516383510089764
16-17	23.720896099710433	27.018110871548657	25.693594147752886	23.567398880988023
18-19	23.705715357986946	26.30294482481857	26.95641658268061	23.034923234513872
20-21	24.42624411155479	25.615506109770177	26.711546382156275	23.246703396518758
22-23	24.12866009187325	25.349674086344937	26.591798731283696	23.929867090498117
24-25	23.952164272691164	25.547430737331943	26.556234187408723	23.94417080256817
26-27	23.71841538688465	25.803102753840097	26.482977240474998	23.995504618800258
28-29	23.81672800958138	25.927482980859136	26.37579388780657	23.879995121752916
30-31	24.006877787440743	25.72484872757555	26.231214383266167	24.037059101717535
32-33	23.789429776498956	25.786094791010743	26.08738382191938	24.337091610570923
34-35	24.19621171062668	25.81465283632422	26.124794251073908	23.86434120197519
36-37	24.343953532715503	25.450544295745146	26.171998385595955	24.033503785943395
38-39	24.081773568094427	25.657862832455447	26.080784075858727	24.1795795235914
40-41	24.252385168045446	25.500497676963047	26.260318849983	23.98679830500851
42-43	24.134503110511897	25.466928410631272	26.0600592892275	24.338509189629335
44-45	23.99588462125145	25.547374876730096	26.242745828910476	24.213994673107983
46-47	24.18986264951698	25.52654533730991	26.02783116200286	24.25576085117025
48-49	23.994147759653934	25.57785538363431	25.98849134717016	24.439505509541586
50-51	23.963102519473406	25.76291287548142	26.108974247932533	24.16501035711264
52-53	24.10081385531076	25.50409062967335	26.197087283765125	24.198008231250768
54-55	23.65009680050254	25.445663534692365	26.487476352553546	24.41676331225155
56-57	23.711489076687144	25.607920492702746	26.319331842159226	24.361258588450887
58-59	23.727756813053965	25.282778949366648	26.407315937404384	24.582148300175007
60-61	23.611493049146322	25.30350575167289	26.6203443846319	24.46465681454889
62-63	23.618538369177507	25.382253053329123	26.5971066878717	24.40210188962167
64-65	23.47574351822579	25.27035627604507	26.489809191529318	24.76409101419982
66-67	23.419671855990117	25.34508942323783	26.497674025927687	24.737564694844362
68-69	23.644379745560787	25.19295511523317	26.433849458158708	24.72881568104734
70-71	23.386945057907464	25.11757384659156	26.507965453187516	24.987515642313458
72-73	23.40448145717943	25.191246894743717	26.503432173247106	24.90083947482975
74-75	23.465747044987257	25.413322888181504	26.388365432435812	24.73256463439543
76-77	23.509677788447377	25.142640708010788	26.503846701040786	24.843834802501053
78-79	23.466339774203824	25.199386712804973	26.488853024727575	24.845420488263624
80-81	23.58519023781578	25.257765721732728	26.39331516895905	24.763728871492447
82-83	23.574452441178835	25.22475758853288	26.472674757086644	24.728115213201637
84-85	23.475096655099296	25.280002730295603	26.453038601938083	24.791862012667014
86-87	23.558261258366752	25.252657043679555	26.408261063911443	24.78082063404225
88-89	23.496041388450994	25.09570200716918	26.39591888988182	25.012337714498006
90-91	23.44224403392051	25.184685577760433	26.44974037609703	24.923330012222024
92-93	23.64642648864103	25.013939620240077	26.458867204477542	24.88076668664135
94-95	23.559540707339206	24.934889036563526	26.54756801956265	24.958002236534615
96-97	23.79063389917918	24.71213635510952	26.50062080548492	24.996608940226377
98	23.8252832304203	24.69656841119441	26.324581835811927	25.15356652257336
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	55.0
1	54.5
2	93.5
3	152.5
4	172.0
5	201.0
6	319.5
7	628.5
8	987.0
9	1132.5
10	1149.5
11	1250.0
12	1448.0
13	1638.5
14	1832.5
15	2033.5
16	2210.0
17	2304.0
18	2366.0
19	2406.5
20	2397.5
21	2501.0
22	2684.5
23	2990.5
24	3550.5
25	4354.0
26	5526.0
27	7336.0
28	9987.0
29	13764.5
30	19484.5
31	27913.5
32	36297.0
33	44128.5
34	53483.0
35	64252.0
36	76157.0
37	87740.5
38	98224.5
39	107821.5
40	117979.5
41	127300.0
42	136820.0
43	146813.0
44	156088.0
45	164393.0
46	171399.0
47	176849.5
48	179622.5
49	179793.5
50	178893.0
51	177685.0
52	174968.0
53	172292.5
54	166844.0
55	158586.5
56	145803.5
57	131221.0
58	119588.5
59	103729.5
60	86367.5
61	72066.0
62	59498.5
63	46144.0
64	33663.0
65	25266.5
66	19112.0
67	14531.0
68	10841.5
69	8127.0
70	5988.5
71	4463.5
72	3526.0
73	2710.0
74	2098.5
75	1636.0
76	1143.5
77	674.5
78	394.0
79	227.0
80	129.0
81	92.0
82	54.5
83	28.0
84	25.0
85	19.5
86	12.0
87	8.0
88	8.5
89	6.0
90	3.5
91	4.5
92	4.5
93	5.0
94	3.0
95	1.5
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.40430875978144176
2	0.026827240074546226
3	0.020576718155966022
4	0.01747645928435024
5	0.023401954063164275
6	0.022776901871306255
7	0.015051256779941125
8	0.01052587891088906
9	0.0
10-11	2.5002087674320805E-5
12-13	0.005425453025327615
14-15	0.0035377954059163945
16-17	0.006125511480208598
18-19	0.037065594977180594
20-21	0.08200684757177225
22-23	0.10132096030018506
24-25	0.06634303964381026
26-27	0.07799401250004376
28-29	0.05821736114965599
30-31	0.05399200833269578
32-33	0.021201770347824042
34-35	0.3556796992548878
36-37	0.5432328599438053
38-39	0.496216434072245
40-41	0.5044671230047709
42-43	0.4542129267793861
44-45	0.42612308127728665
46-47	0.42523550716484826
48-49	0.4144346052895416
50-51	0.41035926499862735
52-53	0.47461463032163187
54-55	0.4764522837656944
56-57	0.44484964494535295
58-59	0.42959837146401725
60-61	0.44456212093709824
62-63	0.42413541530717813
64-65	0.4737020541215191
66-67	0.49574139440643294
68-69	0.43647394557445546
70-71	0.3920452357771874
72-73	0.3773190061370124
74-75	0.36013007086091686
76-77	0.3576048600058105
78-79	0.3564672650166289
80-81	0.3736061961173758
82-83	0.36768070133856173
84-85	0.3687682921523947
86-87	0.35439209173966024
88-89	0.3986832900547196
90-91	0.376431432024574
92-93	0.3655805259739188
94-95	0.37328116897760966
96-97	0.37311865540772654
98	0.36873078902088324
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3999666.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.466870906920924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.33809677384565	46.58521487944158
2	11.651073255436316	13.857057384162205
3	4.172090501373014	7.443035017714202
4	1.8821180123775223	4.476946754945789
5	1.0789527395547527	3.208097163888558
6	0.651739528173528	2.3254146232099644
7	0.44862558886076054	1.867485198482696
8	0.30358057773977615	1.4442389621039793
9	0.22478503467466743	1.2030536374925576
>10	1.1704944220088838	12.266934579429993
>50	0.0578436165174269	2.3436165833888403
>100	0.018826037103116887	1.8505243903815791
>500	9.71428203484843E-4	0.40786574916671353
>1k	8.024841311322027E-4	0.7205150761913419
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.5002087674320805E-5
2	0.0	0.0	0.0	0.0	2.5002087674320805E-5
3	0.0	2.5002087674320805E-5	0.0	0.0	2.5002087674320805E-5
4	0.0	2.5002087674320805E-5	0.0	0.0	2.5002087674320805E-5
5	0.0	5.000417534864161E-5	0.0	0.0	2.5002087674320805E-5
6	0.0	5.000417534864161E-5	0.0	0.0	7.500626302296241E-5
7	0.0	5.000417534864161E-5	0.0	0.0	7.500626302296241E-5
8	0.0	5.000417534864161E-5	0.0	0.0	7.500626302296241E-5
9	0.0	5.000417534864161E-5	0.0	0.0	7.500626302296241E-5
10-11	0.0	5.000417534864161E-5	0.0	5.000417534864161E-5	7.500626302296241E-5
12-13	0.0	7.500626302296241E-5	0.0	7.500626302296241E-5	4.375365343006141E-4
14-15	0.0	1.2501043837160402E-4	0.0	7.500626302296241E-5	5.125427973235765E-4
16-17	0.0	1.6251356988308523E-4	0.0	1.1250939453444364E-4	6.250521918580201E-4
18-19	0.0	1.7501461372024563E-4	0.0	1.5001252604592482E-4	6.500542795323409E-4
20-21	0.0	2.7502296441752884E-4	0.0	1.8751565755740604E-4	6.875574110438221E-4
22-23	0.0	3.0002505209184965E-4	0.0	3.1252609592901005E-4	7.375615863924637E-4
24-25	0.0	3.1252609592901005E-4	0.0	5.375448849978973E-4	7.625636740667846E-4
26-27	0.0	3.2502713976617046E-4	0.0	0.001150096033018757	8.000668055782658E-4
28-29	0.0	3.5002922744049126E-4	0.0	0.00433786221149466	8.250688932525866E-4
30-31	0.0	3.750313151148121E-4	0.0	0.020376701454571457	8.37569937089747E-4
32-33	0.0	4.125344466262933E-4	0.0	0.057654814176983774	0.0010000835069728323
34-35	0.0	4.500375781377745E-4	0.0	0.10810902710376316	0.0010000835069728323
36-37	0.0	5.250438411607369E-4	0.0	0.19217854690866687	0.0010000835069728323
38-39	0.0	6.125511480208597E-4	0.0	0.33674061784158976	0.0010500876823214737
40-41	0.0	6.500542795323409E-4	0.0	0.505954747221393	0.0010500876823214737
42-43	0.0	7.000584548809825E-4	0.0	0.6868823546766155	0.0010625887261586343
44-45	0.0	7.000584548809825E-4	0.0	0.9062006677557576	0.0011000918576701154
46-47	0.0	7.125594987181429E-4	0.0	1.16958516036089	0.0011750981206930778
48-49	0.0	7.250605425553033E-4	0.0	1.4511711727929282	0.0012626054275532007
50-51	0.0	7.375615863924637E-4	0.0	1.7582843167404478	0.0013001085590646818
52-53	0.0	8.000668055782658E-4	0.0	2.0738106631903763	0.001337611690576163
54-55	0.0	8.37569937089747E-4	0.0	2.4288028050342203	0.0014251189974362859
56-57	0.0	8.500709809269074E-4	0.0	2.835486763144723	0.0014501210851106067
58-59	0.0	8.750730686012282E-4	0.0	3.2681603913926813	0.0015001252604592483
60-61	0.0	9.00075156275549E-4	0.0	3.7425250008375697	0.0015376283919707294
62-63	0.0	9.00075156275549E-4	0.0	4.227002954746721	0.0016001336111565315
64-65	0.0	9.250772439498698E-4	0.0	4.743158553739237	0.0016001336111565315
66-67	0.0	9.250772439498698E-4	0.0	5.338145735168887	0.0016251356988308523
68-69	0.0	9.250772439498698E-4	0.0	5.977349108650572	0.0016501377865051731
70-71	2.5002087674320805E-5	9.500793316241906E-4	0.0	6.6536430791971135	0.0017001419618538148
72-73	2.5002087674320805E-5	9.750814192985114E-4	0.0	7.3581269035964505	0.0017876492687139375
74-75	2.5002087674320805E-5	9.750814192985114E-4	0.0	8.080949759304902	0.0018251524002254188
76-77	2.5002087674320805E-5	9.750814192985114E-4	0.0	8.865827796621017	0.0018251524002254188
78-79	2.5002087674320805E-5	9.750814192985114E-4	0.0	9.729987453952404	0.0018876576194112207
80-81	2.5002087674320805E-5	9.875824631356718E-4	0.0	10.621399386848802	0.001925160750922702
82-83	2.5002087674320805E-5	0.0010000835069728323	0.0	11.537425875060567	0.0020376701454571456
84-85	2.5002087674320805E-5	0.0010000835069728323	0.0	12.46209058456381	0.002050171189294306
86	2.5002087674320805E-5	0.0010000835069728323	0.0	13.17057474299104	0.002075173276968627
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5840	0.0	45.86811	1
GGTATCA	3010	0.0	42.96754	1
TCAACGC	7595	0.0	34.77508	4
CAACGCA	7745	0.0	33.80556	5
TATCAAC	7905	0.0	33.41344	2
ATCAACG	7900	0.0	33.37446	3
AACGCAG	8140	0.0	32.046844	6
ACGCAGA	9855	0.0	26.23505	7
GTACATG	12650	0.0	24.995792	1
CGCAGAG	10440	0.0	24.499685	8
TACATGG	12985	0.0	23.73165	2
ACATGGG	13390	0.0	22.738455	3
GCAGAGT	11415	0.0	21.202654	9
CATGGGG	9550	0.0	20.165983	4
AGAGTAC	9800	0.0	18.639627	10-11
TAAGGCG	1280	0.0	18.269794	5
GAGTACA	9730	0.0	17.927803	1
GTATTAA	2340	0.0	17.308823	1
AGTACAT	9625	0.0	17.246798	2
TATTAAG	1985	0.0	16.402063	2
>>END_MODULE