FastQCFastQC Report
Mon 15 Aug 2016
SRR2920850_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920850_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3999666
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32950.046.4653971
GTATCAA60600.044.0168951
TCAACGC79500.033.018134
ATCAACG82200.031.8181023
CAACGCA82050.031.8132425
TATCAAC84250.031.6053262
AACGCAG86450.030.0301156
GTACATG123650.025.1100881
ACGCAGA102700.024.6774397
TACATGG126800.024.1064852
CGCAGAG106750.023.4282268
ACATGGG132100.022.2065033
GCAGAGT122900.019.2681439
CATGGGG94400.019.0070324
TAAGGTG19850.018.8881915
AGAGTAC102250.018.61017810-11
GAGTACA94500.018.566231
TATACCG3451.0968506E-917.8833185
AGTACAT96250.016.9638732
CAGAGTA111300.016.60663810-11
Produced by FastQC (version 0.11.5)