##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920850_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3999666
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.27842699865439	31.0	30.0	34.0	27.0	34.0
2	30.295059387458853	31.0	30.0	34.0	26.0	34.0
3	30.43318867125405	31.0	30.0	34.0	27.0	34.0
4	34.07486050085182	35.0	35.0	37.0	30.0	37.0
5	33.60443997073756	35.0	35.0	37.0	28.0	37.0
6	33.54127144616575	35.0	35.0	37.0	28.0	37.0
7	33.36172420397103	35.0	35.0	37.0	27.0	37.0
8	33.29035224441241	35.0	33.0	37.0	27.0	37.0
9	34.62837246910117	38.0	35.0	39.0	27.0	39.0
10-11	34.437133500647306	38.0	34.0	39.0	27.0	39.0
12-13	34.25743237060294	37.0	34.0	39.0	26.5	39.0
14-15	35.15247685681754	38.0	34.0	40.0	26.0	41.0
16-17	35.02711626420806	38.0	34.0	40.0	25.5	41.0
18-19	34.93858562189943	38.5	34.0	40.0	25.0	41.0
20-21	34.823988803065056	38.0	34.0	40.0	25.0	41.0
22-23	34.67467621046357	38.0	34.0	40.0	24.5	41.0
24-25	34.48987365445015	38.0	33.0	40.0	24.0	41.0
26-27	34.29891008399201	38.0	33.0	40.0	23.0	41.0
28-29	34.241996456704136	38.0	33.0	40.0	23.5	41.0
30-31	34.23274630931683	38.0	33.0	40.0	23.0	41.0
32-33	34.087086521724565	38.0	33.0	40.0	22.0	41.0
34-35	33.91307149146954	38.0	33.0	40.0	21.0	41.0
36-37	33.711979075252785	38.0	33.0	40.0	19.0	41.0
38-39	33.52946596040769	38.0	32.0	40.0	18.0	41.0
40-41	33.29981403447188	38.0	32.0	40.0	17.0	41.0
42-43	33.3229890945894	38.0	32.0	40.0	16.5	41.0
44-45	33.17205849188407	38.0	32.0	40.0	15.0	41.0
46-47	32.92377238499415	38.0	32.0	40.0	14.5	41.0
48-49	32.65411061823662	37.0	31.0	40.0	10.5	41.0
50-51	32.393724751016705	37.0	31.0	40.0	9.0	41.0
52-53	32.12945855978974	37.0	31.0	40.0	8.0	41.0
54-55	31.84171940857062	36.0	30.0	40.0	7.0	41.0
56-57	31.517149056946256	36.0	30.0	39.0	4.0	41.0
58-59	31.183823224239223	35.5	29.5	39.0	2.0	40.5
60-61	30.853995683639585	35.0	29.0	39.0	2.0	40.0
62-63	30.532399705375397	35.0	29.0	39.0	2.0	40.0
64-65	30.130162368557773	35.0	28.0	38.0	2.0	40.0
66-67	29.696478030915582	34.0	28.0	38.0	2.0	40.0
68-69	29.25562972008163	34.0	28.0	37.0	2.0	39.5
70-71	28.821222822105646	34.0	26.5	36.5	2.0	39.0
72-73	28.378505605218038	34.0	26.0	36.0	2.0	39.0
74-75	27.93402324094062	33.0	26.0	35.5	2.0	38.5
76-77	26.4894919975818	31.5	24.5	34.0	2.0	36.0
78-79	27.329808288992133	33.0	26.0	35.0	2.0	37.0
80-81	27.35826266493252	33.0	26.0	35.0	2.0	36.5
82-83	27.018157766173474	33.0	25.5	35.0	2.0	36.0
84-85	26.702284640767502	33.0	25.0	35.0	2.0	36.0
86-87	26.166754549004843	32.5	24.0	35.0	2.0	35.0
88-89	25.800013051089767	32.0	22.5	35.0	2.0	35.0
90-91	25.433919982318525	32.0	20.0	34.5	2.0	35.0
92-93	25.127293253986707	32.0	19.5	34.0	2.0	35.0
94-95	24.748579631399224	32.0	17.5	34.0	2.0	35.0
96-97	24.215513245355986	32.0	6.0	34.0	2.0	35.0
98-99	23.611476683303056	31.0	2.0	34.0	2.0	35.0
100-101	21.401454896483855	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	91417.0
3	42883.0
4	23005.0
5	16494.0
6	14110.0
7	14187.0
8	15923.0
9	18236.0
10	20890.0
11	23417.0
12	25938.0
13	28654.0
14	30173.0
15	32331.0
16	34010.0
17	35218.0
18	34475.0
19	32968.0
20	33060.0
21	34766.0
22	37415.0
23	42043.0
24	48942.0
25	58374.0
26	69767.0
27	81599.0
28	96775.0
29	113911.0
30	133986.0
31	158853.0
32	189833.0
33	231551.0
34	292955.0
35	378891.0
36	504888.0
37	591926.0
38	341706.0
39	24096.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.168940608946556	19.260018047240482	13.963388452070848	24.60765289174211
2	16.06559147688832	20.61477133340634	38.026125181452656	25.293512008252687
3	18.976739324604246	24.445888623613346	29.37915521019725	27.19821684158516
4	13.838392426239102	16.57885458369071	36.10386914957728	33.47888384049291
5	14.552165941273262	37.030518530223866	34.00581931615909	14.41149621234378
6	33.5406880951052	35.40347704251406	17.03994111238229	14.015893749998437
7	29.29545353257209	31.5806051173735	20.899138257817597	18.22480309223681
8	26.901582908434037	34.373535549351736	19.931999423231744	18.792882118982483
9	26.807981548377924	15.708991360066019	19.355500183775113	38.12752690778095
10-11	25.690643171876737	25.3316532746314	27.625751905741495	21.35195164775036
12-13	26.33568607367698	22.628383542606954	27.486337423189266	23.5495929605268
14-15	23.49443611190654	24.183864624405015	24.88398136893474	27.437717894753703
16-17	23.67018012889241	27.144413467467103	25.724117597095564	23.461288806544918
18-19	23.70452413498366	26.400604222199036	26.993704308949006	22.901167333868298
20-21	24.426087928694216	25.638265756961164	26.717109151404237	23.218537162940383
22-23	24.074570588917528	25.43656756893029	26.652507166182748	23.83635467596943
24-25	23.948591058483544	25.62829620668245	26.59705138192394	23.82606135291007
26-27	23.67900411748724	25.852418429570655	26.542106298119283	23.926471154822824
28-29	23.808964616384127	25.983530958084543	26.43545727572358	23.772047149807754
30-31	24.02012295946999	25.76130687950832	26.2763347034398	23.942235457581884
32-33	23.801492064552008	25.82502911462892	26.105352377358106	24.26812644346097
34-35	24.17635577739406	25.829508327904765	26.168176038190456	23.82595985651072
36-37	24.32652873410297	25.520654435901896	26.180449523931465	23.97236730606367
38-39	24.049586446147963	25.673389620975033	26.152182215296104	24.124841717580896
40-41	24.25249352220656	25.5511282401784	26.284461223394533	23.91191701422051
42-43	24.10325341553745	25.540721018036077	26.10118997757274	24.254835588853734
44-45	23.953455628369273	25.57204506736797	26.298792888374933	24.175706415887817
46-47	24.12101028060418	25.56916851669856	26.06072072898989	24.249100473707372
48-49	23.918573250318804	25.649326132073114	26.054509539169356	24.377591078438726
50-51	23.927753467880052	25.761935876382857	26.122108287028666	24.188202368708424
52-53	24.1013614254699	25.530384349095403	26.108744512629485	24.25950971280521
54-55	23.681592549856887	25.485928772997106	26.32312717771668	24.509351499429318
56-57	23.68306070363029	25.621316311088528	26.165488073739724	24.53013491154146
58-59	23.68083840270433	25.353130758517917	26.2364514653045	24.72957937347325
60-61	23.591758383766745	25.37392580614789	26.444534166826195	24.58978164325917
62-63	23.56280617379138	25.47395949826224	26.331993127229396	24.631241200716982
64-65	23.346928794356316	25.318562355008563	26.297052988792746	25.03745586184238
66-67	23.354440970275974	25.403024802661477	26.24035192901391	25.002182298048638
68-69	23.52967672988979	25.30194861946773	26.209173952859615	24.95920069778287
70-71	23.260483935200394	25.163945218346264	26.277648621637304	25.297922224816045
72-73	23.288867244072982	25.221054769555252	26.188655581786136	25.301422404585633
74-75	23.259777858437023	25.41812162971877	26.028783839190655	25.29331667265355
76-77	23.235919207551888	25.09015033557821	26.3194987301684	25.35443172670151
78-79	23.18365343136617	25.143563692485692	26.28502954096651	25.387753335181625
80-81	23.200404302237928	25.241808022203614	26.168104759491058	25.3896829160674
82-83	23.14940452755844	25.17878801101065	26.257185933405257	25.414621528025656
84-85	22.945978287193025	25.174033400259884	26.210625955122328	25.669362357424763
86-87	22.936195314979603	25.16047440858123	26.239245507042476	25.664084769396695
88-89	22.801085497591647	25.03878259817177	26.221879864336707	25.938252039899872
90-91	22.719417381412736	25.07197808912886	26.22183503545788	25.986769494000523
92-93	22.79232721242133	24.907754748508665	26.286123561653422	26.013794477416585
94-95	22.540532026207522	24.805437523361622	26.407329205436024	26.24670124499483
96-97	22.637564365850185	24.57766913643341	26.423162092366287	26.36160440535012
98-99	22.445974146799504	24.635799060567788	26.31826607211548	26.59996072051723
100-101	22.530425597694894	24.461906760384096	26.50065214899126	26.507015492929746
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	54.0
1	51.5
2	82.0
3	110.0
4	104.5
5	118.0
6	187.5
7	418.0
8	634.5
9	652.5
10	550.0
11	482.5
12	519.5
13	581.0
14	638.0
15	690.5
16	780.5
17	901.0
18	1040.0
19	1155.5
20	1314.0
21	1547.5
22	1837.5
23	2220.5
24	2677.5
25	3333.0
26	4404.5
27	5985.5
28	7910.5
29	10832.5
30	15383.0
31	22000.0
32	30691.0
33	38356.0
34	45620.0
35	54617.0
36	65723.0
37	77136.0
38	87513.5
39	97311.0
40	107092.0
41	116955.5
42	126360.5
43	136598.5
44	145987.0
45	154717.0
46	162878.0
47	169273.5
48	175215.0
49	178749.0
50	177907.5
51	176279.0
52	174306.5
53	172485.0
54	169142.0
55	159653.5
56	146276.5
57	132016.0
58	119872.5
59	105520.0
60	87786.0
61	72925.0
62	60434.5
63	47066.0
64	34448.0
65	25766.5
66	19630.0
67	14731.5
68	10973.0
69	8221.0
70	6197.5
71	4680.0
72	3554.0
73	2851.0
74	2230.5
75	1724.0
76	1244.5
77	756.0
78	457.0
79	262.5
80	145.0
81	72.5
82	38.0
83	19.0
84	11.5
85	7.0
86	5.0
87	3.0
88	2.5
89	1.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19801653438062078
2	0.0
3	0.011750981206930779
4	0.014901244253895201
5	0.005675473902070823
6	0.10648389140493231
7	0.14161182458735302
8	0.1248604258455581
9	0.14136180371060983
10-11	0.11419703545246028
12-13	0.11344697282223067
14-15	0.11545964088001347
16-17	0.06529295196148878
18-19	0.07808151980690388
20-21	0.03622802504009085
22-23	0.08229437158002693
24-25	0.05670473484535958
26-27	0.06420536114765582
28-29	0.03896575364042898
30-31	0.023751983290604767
32-33	0.031165102286040886
34-35	0.019026588720158135
36-37	0.023114430054909586
38-39	0.03187766178475902
40-41	0.02512709811269241
42-43	0.012876075152275216
44-45	0.02575215030455043
46-47	0.03279023798487173
48-49	0.009150764088801415
50-51	0.01522627139366137
52-53	0.006375532356951805
54-55	0.01236353235495164
56-57	0.004837903964981075
58-59	0.0039003256771940453
60-61	0.022789402915143413
62-63	0.010138346551937088
64-65	0.007300609600901675
66-67	0.03967831313914712
68-69	0.08951997491790564
70-71	0.02113926512863824
72-73	0.0447037327616856
74-75	0.08576966176675752
76-77	0.25895912308677776
78-79	0.0795566429796888
80-81	0.016938914399352346
82-83	0.01116343214658424
84-85	0.012638555319369166
86-87	0.2717226888445185
88-89	0.631740250310901
90-91	0.26948500199766684
92-93	0.35579220864942224
94-95	0.001275106471390361
96-97	0.0041378455101000935
98-99	0.003062755740104299
100-101	0.004912910228004039
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3999666.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.02106788680593
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.62413838133459	43.691901964100914
2	12.393600762059059	14.133927008306785
3	4.495169559795391	7.6895810589538955
4	2.1252924211910407	4.847457737121938
5	1.1511817866818808	3.2820807404221033
6	0.7243754442926474	2.4782796830727754
7	0.4861637952815993	1.9405105142401502
8	0.3509113728607932	1.6007472971318044
9	0.2525768710534167	1.296198261987651
>10	1.3026631930995622	13.16854689247206
>50	0.07117516797101922	2.7587215547963893
>100	0.020859592517304883	1.9637220583628034
>500	0.0010558057337010378	0.4341401019607801
>1k	8.358461278407363E-4	0.7141851270699718
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.000417534864161E-5
2	0.0	0.0	0.0	2.5002087674320805E-5	5.000417534864161E-5
3	0.0	0.0	0.0	2.5002087674320805E-5	5.000417534864161E-5
4	0.0	0.0	0.0	2.5002087674320805E-5	5.000417534864161E-5
5	0.0	0.0	0.0	2.5002087674320805E-5	5.000417534864161E-5
6	0.0	0.0	0.0	2.5002087674320805E-5	7.500626302296241E-5
7	0.0	0.0	0.0	2.5002087674320805E-5	7.500626302296241E-5
8	0.0	0.0	0.0	2.5002087674320805E-5	7.500626302296241E-5
9	0.0	0.0	0.0	2.5002087674320805E-5	7.500626302296241E-5
10-11	0.0	0.0	0.0	5.000417534864161E-5	7.500626302296241E-5
12-13	0.0	3.7503131511481206E-5	0.0	7.500626302296241E-5	2.0001670139456644E-4
14-15	0.0	5.000417534864161E-5	0.0	7.500626302296241E-5	2.625219205803685E-4
16-17	0.0	5.000417534864161E-5	0.0	1.1250939453444364E-4	3.5002922744049126E-4
18-19	0.0	5.000417534864161E-5	0.0	1.5001252604592482E-4	4.000334027891329E-4
20-21	0.0	5.000417534864161E-5	0.0	1.7501461372024563E-4	4.625386219749349E-4
22-23	0.0	6.250521918580201E-5	0.0	2.875240082546893E-4	5.000417534864161E-4
24-25	0.0	1.0000835069728322E-4	0.0	5.250438411607369E-4	5.375448849978973E-4
26-27	0.0	1.0000835069728322E-4	0.0	0.0011250939453444362	6.250521918580201E-4
28-29	0.0	1.0000835069728322E-4	0.0	0.00433786221149466	6.625553233695013E-4
30-31	0.0	1.1250939453444364E-4	0.0	0.02010167849015393	6.875574110438221E-4
32-33	0.0	1.8751565755740604E-4	0.0	0.056992258853614274	7.250605425553033E-4
34-35	0.0	2.0001670139456644E-4	0.0	0.10684642167620997	7.75064717903945E-4
36-37	0.0	2.5002087674320803E-4	0.0	0.19085343626192786	7.75064717903945E-4
38-39	0.0	3.1252609592901005E-4	0.0	0.33429041324950637	8.125678494154262E-4
40-41	0.0	3.3752818360333086E-4	0.0	0.5029419956566373	8.250688932525866E-4
42-43	0.0	3.875323589519725E-4	0.0	0.6840321166817429	8.625720247640678E-4
44-45	0.0	4.000334027891329E-4	0.0	0.9032504214101877	9.375782877870302E-4
46-47	0.0	4.000334027891329E-4	0.0	1.1652723002370697	9.750814192985114E-4
48-49	0.0	4.000334027891329E-4	0.0	1.4443956070331874	0.001025085594647153
50-51	0.0	4.125344466262933E-4	0.0	1.747783439917233	0.0010750897699957945
52-53	0.0	4.500375781377745E-4	0.0	2.0585468886652034	0.0012376033398788797
54-55	0.0	4.750396658120953E-4	0.0	2.4077135440809307	0.0013501127344133234
56-57	0.0	4.750396658120953E-4	0.0	2.808309493842736	0.0013751148220876443
58-59	0.0	4.750396658120953E-4	0.0	3.235407656539321	0.0013751148220876443
60-61	0.0	5.125427973235765E-4	0.0	3.7015465791393583	0.0013876158659248045
62-63	0.0	5.250438411607369E-4	0.0	4.178523906746213	0.0014876242166220878
64-65	0.0	5.250438411607369E-4	0.0	4.687028866910387	0.001525127348133569
66-67	0.0	6.375532356951805E-4	0.0	5.271977710138796	0.001525127348133569
68-69	0.0	6.500542795323409E-4	0.0	5.899805133728666	0.001525127348133569
70-71	2.5002087674320805E-5	6.625553233695013E-4	0.0	6.565598227451993	0.00155012943580789
72-73	2.5002087674320805E-5	6.750563672066617E-4	0.0	7.261043797157063	0.0016876409180166542
74-75	2.5002087674320805E-5	6.750563672066617E-4	0.0	7.978541208190884	0.0017626471810396169
76-77	2.5002087674320805E-5	6.750563672066617E-4	0.0	8.758118802920045	0.001800150312551098
78-79	2.5002087674320805E-5	6.750563672066617E-4	0.0	9.615840422675294	0.001837653444062579
80-81	2.5002087674320805E-5	6.875574110438221E-4	0.0	10.500501791899623	0.0019126597070855417
82-83	2.5002087674320805E-5	7.000584548809825E-4	0.0	11.406864973225264	0.0021001753646429475
84-85	5.000417534864161E-5	7.125594987181429E-4	0.0	12.322991469787727	0.0021001753646429475
86-87	5.000417534864161E-5	7.250605425553033E-4	0.0	13.268145390140077	0.002150179539991589
88-89	5.000417534864161E-5	7.250605425553033E-4	0.0	14.266878784378495	0.0022001837153402307
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3295	0.0	46.465397	1
GTATCAA	6060	0.0	44.016895	1
TCAACGC	7950	0.0	33.01813	4
ATCAACG	8220	0.0	31.818102	3
CAACGCA	8205	0.0	31.813242	5
TATCAAC	8425	0.0	31.605326	2
AACGCAG	8645	0.0	30.030115	6
GTACATG	12365	0.0	25.110088	1
ACGCAGA	10270	0.0	24.677439	7
TACATGG	12680	0.0	24.106485	2
CGCAGAG	10675	0.0	23.428226	8
ACATGGG	13210	0.0	22.206503	3
GCAGAGT	12290	0.0	19.268143	9
CATGGGG	9440	0.0	19.007032	4
TAAGGTG	1985	0.0	18.888191	5
AGAGTAC	10225	0.0	18.610178	10-11
GAGTACA	9450	0.0	18.56623	1
TATACCG	345	1.0968506E-9	17.883318	5
AGTACAT	9625	0.0	16.963873	2
CAGAGTA	11130	0.0	16.606638	10-11
>>END_MODULE