FastQCFastQC Report
Mon 15 Aug 2016
SRR2920849_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920849_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4688964
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34800.044.3024981
GTATCAA64150.043.5466421
TCAACGC82150.033.7154
CAACGCA84750.032.8435
ATCAACG87350.031.760423
AACGCAG90500.031.0053316
TATCAAC91950.030.2226982
GTACATG156000.027.0821
TACATGG163750.025.2322392
ACGCAGA111150.024.7872927
ACATGGG169050.024.1417433
CGCAGAG115200.023.7957238
CATGGGG125750.022.3170744
GCAGAGT131000.019.631189
GAGTACA116100.018.9081381
ATGGGGG74800.017.9622125
AGAGTAC114150.017.91094210-11
AGTACAT115200.017.3956762
GTATACG3704.1854946E-916.1697861
GTATAGG12000.016.1075941
Produced by FastQC (version 0.11.5)