##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920849_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4688964
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.687112121142324	31.0	30.0	33.0	22.0	34.0
2	28.72189549759819	31.0	28.0	34.0	20.0	34.0
3	28.79813430002875	31.0	28.0	34.0	22.0	34.0
4	32.27816741608594	35.0	32.0	37.0	23.0	37.0
5	32.24942609924069	35.0	32.0	37.0	23.0	37.0
6	32.3709427071737	35.0	33.0	37.0	24.0	37.0
7	32.20666057576898	35.0	32.0	37.0	23.0	37.0
8	32.15001117517644	35.0	32.0	37.0	23.0	37.0
9	33.419766711793905	37.0	33.0	39.0	22.0	39.0
10-11	33.23668351900334	37.0	33.0	39.0	21.5	39.0
12-13	33.05655737600033	37.0	32.0	39.0	19.0	39.0
14-15	33.821807759667166	38.0	33.0	40.0	18.0	41.0
16-17	33.70279170409498	38.0	32.0	40.0	17.5	41.0
18-19	33.5827410916356	38.0	32.0	40.0	17.5	41.0
20-21	33.487730018827186	38.0	32.0	40.0	18.0	41.0
22-23	33.2763476111141	38.0	32.0	40.0	16.5	41.0
24-25	33.081087528076566	38.0	32.0	40.0	15.5	41.0
26-27	33.118123534324425	38.0	32.0	40.0	14.0	41.0
28-29	32.95988516866412	38.0	32.0	40.0	10.0	41.0
30-31	32.71668037971714	38.0	31.0	40.0	9.5	41.0
32-33	32.47794982004554	37.5	31.0	40.0	9.0	41.0
34-35	32.162996559581174	37.0	30.5	40.0	8.0	41.0
36-37	31.864308299231986	37.0	30.0	40.0	6.5	41.0
38-39	31.670497256963372	37.0	30.0	40.0	2.0	41.0
40-41	31.480833186179293	37.0	30.0	40.0	2.0	41.0
42-43	31.254215643370262	37.0	30.0	40.0	2.0	41.0
44-45	30.98559660513495	36.0	29.5	40.0	2.0	41.0
46-47	30.74045535431707	36.0	29.0	39.0	2.0	40.5
48-49	30.448318007986416	36.0	28.0	39.0	2.0	40.0
50-51	29.30506077675154	34.0	26.5	38.0	2.0	39.5
52-53	29.759053385779886	34.5	27.5	38.5	2.0	39.5
54-55	30.413605222816813	36.0	28.0	39.0	2.0	40.0
56-57	30.370022674518296	36.0	28.0	39.0	2.0	41.0
58-59	30.12745523318157	35.0	28.0	39.0	2.0	41.0
60-61	29.802792045321738	35.0	28.0	39.0	2.0	40.0
62-63	29.400862429312745	35.0	27.0	39.0	2.0	40.0
64-65	28.985055227551328	34.0	26.0	38.0	2.0	40.0
66-67	28.60843482696817	34.0	26.0	38.0	2.0	40.0
68-69	28.162208005862276	34.0	26.0	37.0	2.0	40.0
70-71	27.836438710128718	34.0	26.0	36.5	2.0	39.0
72-73	27.376044793690035	33.5	25.0	36.0	2.0	39.0
74-75	26.937703509773158	33.0	24.0	35.5	2.0	38.5
76-77	26.521889696743244	33.0	22.5	35.0	2.0	37.5
78-79	26.06428957441345	32.5	21.5	35.0	2.0	37.0
80-81	25.61772579188068	32.0	20.0	35.0	2.0	36.5
82-83	25.14317427047851	32.0	18.0	35.0	2.0	36.0
84-85	24.742700839673752	32.0	15.0	35.0	2.0	35.5
86-87	24.28343297154766	31.0	7.5	34.0	2.0	35.0
88-89	23.75907087365141	31.0	2.0	34.0	2.0	35.0
90-91	23.266269691983133	31.0	2.0	34.0	2.0	35.0
92-93	22.72976130761507	30.5	2.0	34.0	2.0	35.0
94-95	22.094121963828258	30.0	2.0	34.0	2.0	35.0
96-97	21.451065096682335	29.5	2.0	34.0	2.0	35.0
98	21.0629556976765	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	230630.0
3	40523.0
4	30477.0
5	24892.0
6	23108.0
7	23177.0
8	26368.0
9	29756.0
10	32609.0
11	35494.0
12	36763.0
13	36160.0
14	34113.0
15	35549.0
16	38050.0
17	40527.0
18	41973.0
19	41901.0
20	44013.0
21	46364.0
22	50712.0
23	56401.0
24	64498.0
25	73529.0
26	85452.0
27	99215.0
28	115257.0
29	133694.0
30	155621.0
31	182905.0
32	217861.0
33	264807.0
34	331952.0
35	419024.0
36	533614.0
37	602254.0
38	369849.0
39	39870.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.364524671619314	17.813896090352788	12.847644766198263	24.973934471829637
2	15.999452188982854	19.425937571729538	39.53062323316014	25.04398700612746
3	19.59748883270311	23.63723355216777	28.610805544943396	28.154472070185722
4	13.493123408354382	15.275809162290207	36.234989018058506	34.99607841129691
5	14.622019571767591	36.86390955390235	33.88087353011759	14.633197344212462
6	33.83589138210147	34.45280097647488	17.362442706014633	14.348864935409011
7	29.742932998423928	31.146446859616404	20.73465631934249	18.375963822617177
8	26.947793023442813	34.56276702457282	19.83594658644723	18.653493365537138
9	27.20319456494014	15.87941387479196	19.223329503062935	37.69406205720496
10-11	26.038782344968137	25.18486228353025	27.3176150763383	21.458740295163313
12-13	26.503120424842297	22.31774256620352	27.38148671983914	23.79765028911504
14-15	23.660820541869526	23.939878911551858	24.685218234756668	27.714082311821947
16-17	23.98997878174567	26.93532458106558	25.393335375853805	23.68136126133495
18-19	23.95891392796678	26.42419985814512	26.604969358470953	23.01191685541715
20-21	24.50498055652375	25.648676159559663	26.509966982695637	23.33637630122095
22-23	24.268135968407123	25.297784337863682	26.36266802555516	24.071411668174033
24-25	24.055732763923775	25.48891707576706	26.3568604607612	24.09848969954796
26-27	23.841388797794643	25.806847496066705	26.314272978453868	24.037490727684787
28-29	23.875197790930052	26.07751377201041	26.14496808137196	23.902320355687575
30-31	24.33253787377255	25.733445758189756	26.022122147550903	23.911894220486797
32-33	23.9748803585351	25.76177509857689	25.987011421800293	24.27633312108772
34-35	24.261722220450537	25.86480115935354	26.07920494133566	23.79427167886027
36-37	24.348527479002968	25.603546084538802	26.08394695969483	23.9639794767634
38-39	24.198547014656416	25.75420861745415	26.05805790015101	23.989186467738424
40-41	24.387642140771113	25.55670674440067	26.09899564340459	23.95665547142362
42-43	24.15588430571151	25.516451040871956	25.98723063379565	24.340434019620886
44-45	23.981517288559758	25.580334371038525	26.186625651091376	24.251522689310338
46-47	24.10472551655569	25.490392835812198	26.05755806027351	24.3473235873586
48-49	24.02002162827571	25.62313538039177	26.018939893246397	24.337903098086127
50-51	23.988210760759287	25.721871394497818	26.16346995537543	24.126447889367466
52-53	24.10444092727998	25.52740323976276	26.22294679810176	24.1452090348555
54-55	23.60494581143558	25.405474036097136	26.519620921125238	24.469959231342045
56-57	23.707574687744216	25.523493305866708	26.360613440366343	24.408318566022732
58-59	23.798415258210785	25.406033054116108	26.39510976092217	24.40044192675094
60-61	23.879930513349873	25.38152211239759	26.525161918076616	24.213385456175917
62-63	23.774472636739198	25.399645483035787	26.516538841797022	24.309343038427993
64-65	23.563384893362795	25.251657286661306	26.513522293441312	24.671435526534584
66-67	23.553878161554646	25.353189004958093	26.389560158458437	24.703372675028824
68-69	23.604365591513016	25.281080473188993	26.43961163800721	24.674942297290784
70-71	23.49743337529589	25.125248009633367	26.50570873132012	24.87160988375062
72-73	23.460343068434288	25.335796116492382	26.411572511880404	24.792288303192926
74-75	23.482825884711666	25.4268044644106	26.39159129682012	24.69877835405761
76-77	23.48950538856945	25.15604292020397	26.458430362970752	24.89602132825583
78-79	23.43558590784052	25.183241843061232	26.52621451797852	24.854957731119733
80-81	23.55851668923695	25.290120552287632	26.364032201508174	24.787330556967238
82-83	23.62032185666796	25.24958785105976	26.435478662709812	24.69461162956247
84-85	23.567250723417228	25.295018085965815	26.326018745700065	24.8117124449169
86-87	23.485021814761268	25.39020973632872	26.3909599030027	24.73380854590731
88-89	23.447734776060024	25.139656011953633	26.455007081300696	24.95760213068565
90-91	23.578525724392655	25.099926378487364	26.444487688252394	24.877060208867594
92-93	23.781857630322946	24.965360449328433	26.419001460501494	24.833780459847127
94-95	23.645921392978806	24.96505800403441	26.453922055747498	24.93509854723929
96-97	23.715297196481757	24.765825816343735	26.464901835385284	25.053975151789228
98	23.66877622905288	24.68164445257873	26.327980760277292	25.321598558091097
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	42.0
1	49.5
2	109.5
3	188.0
4	198.0
5	219.5
6	353.0
7	627.0
8	943.0
9	1148.5
10	1218.0
11	1356.0
12	1589.0
13	1852.0
14	2079.5
15	2249.5
16	2519.5
17	2665.5
18	2665.5
19	2655.5
20	2637.0
21	2669.5
22	2826.0
23	3178.5
24	3712.0
25	4639.0
26	6117.5
27	8113.5
28	11291.0
29	16110.5
30	23265.5
31	32930.0
32	42531.5
33	52042.0
34	63344.5
35	76364.5
36	89861.0
37	101870.0
38	112867.5
39	123407.5
40	133428.5
41	144345.5
42	157199.0
43	169395.5
44	179934.0
45	190032.5
46	199497.0
47	207581.5
48	213058.0
49	214790.0
50	214430.0
51	213105.5
52	210919.0
53	207808.5
54	200239.0
55	188912.5
56	174091.5
57	157174.5
58	140440.5
59	120745.0
60	100645.5
61	82962.0
62	67585.5
63	52562.0
64	38671.0
65	28837.0
66	21853.5
67	16955.0
68	13017.5
69	9879.0
70	7400.5
71	5621.0
72	4374.5
73	3365.0
74	2643.0
75	1955.0
76	1301.5
77	838.0
78	500.5
79	273.5
80	165.0
81	123.0
82	77.5
83	40.0
84	29.5
85	22.5
86	16.0
87	10.5
88	9.0
89	8.0
90	6.0
91	6.0
92	6.5
93	6.5
94	5.0
95	3.0
96	2.0
97	2.0
98	1.5
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.40527075916982946
2	0.02593323386573239
3	0.020153705594668673
4	0.017338584813191144
5	0.023224746447189613
6	0.023054133066494006
7	0.015589797661061163
8	0.010471396240192929
9	0.0
10-11	1.0663336293475488E-5
12-13	0.0055342715363137784
14-15	0.0034122676139121564
16-17	0.006664585183422181
18-19	0.03795081386847926
20-21	0.08200105609682651
22-23	0.10116307141620196
24-25	0.06770152212727588
26-27	0.07774638491572979
28-29	0.06033315674848431
30-31	0.05490551857510529
32-33	0.021305345914364025
34-35	0.35626206556501605
36-37	0.5452590380305756
38-39	0.4975192814446859
40-41	0.5062632172053357
42-43	0.45611354661712056
44-45	0.4301269960699208
46-47	0.42931658251161664
48-49	0.418290692784163
50-51	0.41483577182507686
52-53	0.47613929217626755
54-55	0.47748287254924543
56-57	0.44777481763562277
58-59	0.43329400694908304
60-61	0.44822267775994873
62-63	0.4281542788556278
64-65	0.47558479868900677
66-67	0.49607973104506664
68-69	0.4401611955220812
70-71	0.3951939063724951
72-73	0.38016926553498814
74-75	0.3614337836673517
76-77	0.3593970864352979
78-79	0.35810682274378736
80-81	0.37598923770794573
82-83	0.37135068642028385
84-85	0.3707855295967297
86-87	0.3554943053518858
88-89	0.4034579919999386
90-91	0.3790816052330536
92-93	0.36854622897509987
94-95	0.3764370978322717
96-97	0.37538142753921766
98	0.370636242888621
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	4688964.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.71218804142535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.61024742992281	44.79057193617254
2	11.813437668947161	13.635586723318724
3	4.327691605626852	7.4928165518770475
4	2.002777120095728	4.62338599040116
5	1.1189863629406918	3.228957569691192
6	0.7014996326633214	2.429104722675385
7	0.452286429346488	1.8271707621320552
8	0.32998778804302326	1.5235453819930376
9	0.2502455321444971	1.299799548688479
>10	1.2953568843702676	13.41217241274858
>50	0.07292792239357263	2.8395178476639993
>100	0.023256608088710527	2.238371710553034
>500	8.90753878348209E-4	0.3401452326331172
>1k	4.082615387313852E-4	0.3188536094517594
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.1326672586950976E-5
2	0.0	0.0	0.0	0.0	2.1326672586950976E-5
3	0.0	0.0	0.0	0.0	2.1326672586950976E-5
4	0.0	0.0	0.0	0.0	2.1326672586950976E-5
5	0.0	0.0	0.0	0.0	2.1326672586950976E-5
6	0.0	0.0	0.0	0.0	2.1326672586950976E-5
7	0.0	0.0	0.0	0.0	2.1326672586950976E-5
8	0.0	0.0	0.0	0.0	2.1326672586950976E-5
9	0.0	0.0	0.0	2.1326672586950976E-5	4.265334517390195E-5
10-11	0.0	0.0	0.0	4.265334517390195E-5	4.265334517390195E-5
12-13	0.0	0.0	0.0	6.398001776085294E-5	7.464335405432841E-5
14-15	2.1326672586950976E-5	1.0663336293475488E-5	0.0	6.398001776085294E-5	9.59700266412794E-5
16-17	2.1326672586950976E-5	2.1326672586950976E-5	0.0	8.53066903478039E-5	1.0663336293475489E-4
18-19	2.1326672586950976E-5	2.1326672586950976E-5	0.0	1.0663336293475489E-4	1.0663336293475489E-4
20-21	2.1326672586950976E-5	2.1326672586950976E-5	0.0	1.4928670810865683E-4	1.1729669922823038E-4
22-23	2.1326672586950976E-5	2.1326672586950976E-5	0.0	1.5995004440213232E-4	1.2796003552170587E-4
24-25	2.1326672586950976E-5	2.1326672586950976E-5	0.0	3.945434428585931E-4	1.2796003552170587E-4
26-27	2.1326672586950976E-5	2.1326672586950976E-5	0.0	7.784235494237106E-4	1.2796003552170587E-4
28-29	2.1326672586950976E-5	3.199000888042646E-5	0.0	0.0038814544108250774	1.2796003552170587E-4
30-31	2.1326672586950976E-5	5.3316681467377444E-5	0.0	0.018991401938679844	1.3862337181518134E-4
32-33	2.1326672586950976E-5	7.464335405432841E-5	0.0	0.05546001206236602	1.4928670810865683E-4
34-35	2.1326672586950976E-5	8.53066903478039E-5	0.0	0.1017602182486366	1.4928670810865683E-4
36-37	2.1326672586950976E-5	8.53066903478039E-5	0.0	0.17616897890450856	1.4928670810865683E-4
38-39	2.1326672586950976E-5	8.53066903478039E-5	0.0	0.30815975554514813	1.4928670810865683E-4
40-41	2.1326672586950976E-5	8.53066903478039E-5	0.0	0.4680244932569327	1.4928670810865683E-4
42-43	2.1326672586950976E-5	1.2796003552170587E-4	0.0	0.6363452566494432	1.4928670810865683E-4
44-45	2.1326672586950976E-5	1.2796003552170587E-4	0.0	0.8371572057281736	1.5995004440213232E-4
46-47	2.1326672586950976E-5	1.2796003552170587E-4	0.0	1.073691331390047	1.706133806956078E-4
48-49	2.1326672586950976E-5	1.2796003552170587E-4	0.0	1.331904019736556	1.706133806956078E-4
50-51	2.1326672586950976E-5	1.2796003552170587E-4	0.0	1.6209657399800894	1.706133806956078E-4
52-53	2.1326672586950976E-5	1.2796003552170587E-4	0.0	1.9220450402263698	1.706133806956078E-4
54-55	2.1326672586950976E-5	1.2796003552170587E-4	0.0	2.2556688428403375	1.919400532825588E-4
56-57	2.1326672586950976E-5	1.2796003552170587E-4	0.0	2.63493172479038	1.919400532825588E-4
58-59	2.1326672586950976E-5	1.2796003552170587E-4	0.0	3.044937005274513	1.919400532825588E-4
60-61	2.1326672586950976E-5	1.2796003552170587E-4	0.0	3.4926371795560813	2.1326672586950978E-4
62-63	2.1326672586950976E-5	1.2796003552170587E-4	0.0	3.956982821791765	2.3459339845646073E-4
64-65	2.1326672586950976E-5	1.3862337181518134E-4	0.0	4.4450117339352575	2.5592007104341174E-4
66-67	2.1326672586950976E-5	1.919400532825588E-4	0.0	5.000390278108341	2.6658340733688723E-4
68-69	2.1326672586950976E-5	1.919400532825588E-4	0.0	5.606675589746477	2.7724674363036267E-4
70-71	2.1326672586950976E-5	1.919400532825588E-4	0.0	6.251988712218733	2.7724674363036267E-4
72-73	2.1326672586950976E-5	1.919400532825588E-4	0.0	6.925687635904222	2.7724674363036267E-4
74-75	2.1326672586950976E-5	1.919400532825588E-4	0.0	7.6116174063183255	2.8791007992383816E-4
76-77	2.1326672586950976E-5	1.919400532825588E-4	0.0	8.351461005032242	2.9857341621731366E-4
78-79	2.1326672586950976E-5	1.919400532825588E-4	0.0	9.162013186708194	2.9857341621731366E-4
80-81	2.1326672586950976E-5	1.919400532825588E-4	0.0	10.006645391178093	2.9857341621731366E-4
82-83	2.1326672586950976E-5	1.919400532825588E-4	0.0	10.869511900709837	2.9857341621731366E-4
84-85	2.1326672586950976E-5	2.1326672586950978E-4	0.0	11.733860613986373	2.9857341621731366E-4
86	4.265334517390195E-5	2.1326672586950978E-4	2.1326672586950976E-5	12.400841635806971	2.9857341621731366E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3480	0.0	44.302498	1
GTATCAA	6415	0.0	43.546642	1
TCAACGC	8215	0.0	33.715	4
CAACGCA	8475	0.0	32.843	5
ATCAACG	8735	0.0	31.76042	3
AACGCAG	9050	0.0	31.005331	6
TATCAAC	9195	0.0	30.222698	2
GTACATG	15600	0.0	27.082	1
TACATGG	16375	0.0	25.232239	2
ACGCAGA	11115	0.0	24.787292	7
ACATGGG	16905	0.0	24.141743	3
CGCAGAG	11520	0.0	23.795723	8
CATGGGG	12575	0.0	22.317074	4
GCAGAGT	13100	0.0	19.63118	9
GAGTACA	11610	0.0	18.908138	1
ATGGGGG	7480	0.0	17.962212	5
AGAGTAC	11415	0.0	17.910942	10-11
AGTACAT	11520	0.0	17.395676	2
GTATACG	370	4.1854946E-9	16.169786	1
GTATAGG	1200	0.0	16.107594	1
>>END_MODULE