FastQCFastQC Report
Mon 15 Aug 2016
SRR2920849_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920849_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4688964
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA66600.043.7692071
GGTATCA36800.041.609311
TCAACGC84600.033.7779354
CAACGCA87750.032.344875
ATCAACG89300.032.0001533
TATCAAC93750.030.681452
AACGCAG93600.030.3236396
GTACATG155550.026.2606561
TACATGG164900.024.5816922
ACGCAGA115650.024.3768867
ACATGGG169250.023.4468563
CGCAGAG119300.023.310078
CATGGGG126150.020.0561224
GAGTACA122000.019.91791
GCAGAGT138250.019.016529
GTGTACG5550.018.8500351
AGTACAT120750.018.6715772
AGAGTAC118900.018.33794410-11
GTGGTAT27550.017.7786221
GTATATA16550.017.5272711
Produced by FastQC (version 0.11.5)