##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920849_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4688964
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.306550018298285	31.0	30.0	34.0	27.0	34.0
2	30.324138978247646	31.0	30.0	34.0	26.0	34.0
3	30.463238361394968	31.0	30.0	34.0	27.0	34.0
4	34.106278700369636	35.0	35.0	37.0	30.0	37.0
5	33.633999109398154	35.0	35.0	37.0	28.0	37.0
6	33.572092470746206	35.0	35.0	37.0	28.0	37.0
7	33.38962337949278	35.0	35.0	37.0	27.0	37.0
8	33.32140468555527	35.0	35.0	37.0	27.0	37.0
9	34.663140727887864	38.0	35.0	39.0	27.0	39.0
10-11	34.47256419541715	38.0	34.0	39.0	27.0	39.0
12-13	34.28358140518887	37.0	34.0	39.0	27.0	39.0
14-15	35.18372022050073	38.0	34.0	40.0	26.0	41.0
16-17	35.05225738563998	38.0	34.0	40.0	25.5	41.0
18-19	34.95995746608419	38.5	34.0	40.0	25.0	41.0
20-21	34.841034288171116	38.0	34.0	40.0	25.0	41.0
22-23	34.696530086390084	38.0	34.0	40.0	24.5	41.0
24-25	34.51196714242208	38.0	33.0	40.0	24.0	41.0
26-27	34.32574178432592	38.0	33.0	40.0	23.5	41.0
28-29	34.272852489377186	38.0	33.0	40.0	23.5	41.0
30-31	34.26916041581893	38.0	33.0	40.0	23.0	41.0
32-33	34.125569635424796	38.0	33.0	40.0	22.5	41.0
34-35	33.95883077797142	38.0	33.0	40.0	21.5	41.0
36-37	33.77092263877479	38.0	33.0	40.0	20.0	41.0
38-39	33.59343791080503	38.0	32.5	40.0	18.5	41.0
40-41	33.3583979318246	38.0	32.0	40.0	17.5	41.0
42-43	33.394275046684086	38.0	32.0	40.0	17.5	41.0
44-45	33.25667535515308	38.0	32.0	40.0	15.5	41.0
46-47	33.01377980295861	38.0	32.0	40.0	15.0	41.0
48-49	32.75717866462613	37.0	31.0	40.0	12.5	41.0
50-51	32.50020932129144	37.0	31.0	40.0	9.0	41.0
52-53	32.2356324339449	37.0	31.0	40.0	8.5	41.0
54-55	31.952626955549242	36.5	30.5	40.0	7.5	41.0
56-57	31.626897327426697	36.0	30.0	39.0	6.5	41.0
58-59	31.292216468285957	36.0	29.5	39.0	2.0	40.5
60-61	30.960889441676244	35.0	29.0	39.0	2.0	40.0
62-63	30.632739022948353	35.0	29.0	39.0	2.0	40.0
64-65	30.233301855164594	35.0	28.0	38.0	2.0	40.0
66-67	29.806470043276086	34.0	28.0	38.0	2.0	40.0
68-69	29.359163666003834	34.0	28.0	37.0	2.0	39.5
70-71	28.9296342432998	34.0	27.0	36.5	2.0	39.0
72-73	28.482695431229587	34.0	26.0	36.0	2.0	39.0
74-75	28.038055741097608	33.0	26.0	35.5	2.0	38.5
76-77	26.587493740621596	31.5	24.5	34.5	2.0	36.0
78-79	27.428081128368653	33.0	26.0	35.0	2.0	37.0
80-81	27.456422783369632	33.0	26.0	35.0	2.0	36.5
82-83	27.11786889385374	33.0	26.0	35.0	2.0	36.0
84-85	26.797743915287043	33.0	25.0	35.0	2.0	36.0
86-87	26.258552742140907	32.5	24.0	35.0	2.0	35.0
88-89	25.890312870817517	32.0	23.5	35.0	2.0	35.0
90-91	25.522869230815164	32.0	21.0	35.0	2.0	35.0
92-93	25.203870513827788	32.0	20.0	34.0	2.0	35.0
94-95	24.817772220046905	32.0	18.0	34.0	2.0	35.0
96-97	24.282929662074608	32.0	6.5	34.0	2.0	35.0
98-99	23.68270976275356	31.5	2.0	34.0	2.0	35.0
100-101	21.462657316200335	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	105712.0
3	49951.0
4	27308.0
5	19235.0
6	16438.0
7	16221.0
8	18301.0
9	21216.0
10	24034.0
11	26752.0
12	29283.0
13	32526.0
14	35092.0
15	36745.0
16	39421.0
17	40029.0
18	39513.0
19	38487.0
20	38863.0
21	40378.0
22	43683.0
23	48966.0
24	57703.0
25	67634.0
26	80345.0
27	95613.0
28	112963.0
29	132850.0
30	156696.0
31	185572.0
32	222215.0
33	271832.0
34	343601.0
35	442702.0
36	589142.0
37	700543.0
38	410994.0
39	30405.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.644316119762486	18.945697632973747	13.468065702009232	24.941920545254536
2	16.112237159423703	20.427092210560797	38.23426667383243	25.22640395618307
3	19.432190207279625	24.31472905154657	28.90015167217992	27.352929068993888
4	14.00519590214811	16.164353466387674	35.80249599535867	34.02795463610555
5	14.692199165434083	37.041076724398096	33.827240803085736	14.43948330708208
6	33.91657025519453	34.87758216040122	17.059247633978142	14.146599950426106
7	29.674362658747988	31.260817416531832	20.745434260007553	18.319385664712627
8	27.04835823215684	34.63433716558054	19.7361478474011	18.58115675486152
9	27.093265286683106	15.68480128689497	19.172056849251582	38.049876577170345
10-11	26.040703872399906	25.24962993471094	27.318109681095848	21.391556511793304
12-13	26.45142356546531	22.349904143367958	27.472591878352286	23.726080412814447
14-15	23.623993586883728	23.995764729846197	24.705326210922472	27.6749154723476
16-17	24.03078408724114	27.00135780766081	25.39021364796769	23.577644457130358
18-19	23.9958456735157	26.415371605613892	26.648401489273965	22.940381231596444
20-21	24.525730875439773	25.61816515203995	26.539005856006685	23.317098116513595
22-23	24.24689948755953	25.325344629449702	26.423330726449677	24.00442515654109
24-25	24.035529379756255	25.53183907218172	26.398787946684592	24.03384360137743
26-27	23.862049876629417	25.797715350066436	26.351581196453516	23.988653576850634
28-29	23.866478419793015	26.07142038026651	26.207282423114897	23.854818776825578
30-31	24.312772248081195	25.714500556761994	26.118483644849842	23.85424355030697
32-33	23.99872682517281	25.75916622408837	26.01959319632268	24.222513754416138
34-35	24.26450543613897	25.857773028133114	26.11481910466354	23.762902431064383
36-37	24.312394974452562	25.646877378157036	26.16286045994457	23.877867187445837
38-39	24.192933501441075	25.765476899052153	26.099377702683963	23.94221189682281
40-41	24.359574798730364	25.547007249770605	26.160759511357508	23.93265844014153
42-43	24.128195606514957	25.563785646595143	26.038952714931114	24.269066031958786
44-45	23.937138958215762	25.565078156590072	26.261021710584632	24.236761174609537
46-47	24.02635193903994	25.526160894842064	26.112083102839378	24.335404063278617
48-49	23.94519478007133	25.644212905469445	26.102224118575297	24.308368195883933
50-51	23.945621326575992	25.706699856129227	26.214854844093775	24.132823973201
52-53	24.12581778542603	25.50788835216763	26.15133534606471	24.214958516341632
54-55	23.64481491178442	25.40496728875532	26.421482081468568	24.52873571799169
56-57	23.692859194126008	25.532704926520168	26.212717693288525	24.561718186065303
58-59	23.73242873788909	25.423938472950276	26.267064735866825	24.576568053293812
60-61	23.832377519829546	25.435710276144402	26.403629435536214	24.328282768489842
62-63	23.724855433137506	25.438435589469353	26.318602073845554	24.518106903547583
64-65	23.45250591298656	25.275163558236375	26.340967455139523	24.931363073637545
66-67	23.479309170287696	25.387935388888007	26.218258946892085	24.914496493932216
68-69	23.513180837199315	25.32856170558476	26.21350291246676	24.944754544749166
70-71	23.381540925791835	25.150585399239688	26.264706707370987	25.20316696759749
72-73	23.344559036609347	25.352394554737355	26.081197594211737	25.221848814441554
74-75	23.26139122000698	25.371766969965787	26.077904358747332	25.288937451279892
76-77	23.19986922543762	25.029323244847035	26.31016984977069	25.460637679944654
78-79	23.079334117152634	25.03571635531069	26.34220873334922	25.54274079418746
80-81	23.12646898282558	25.147279559523255	26.16459049360809	25.561660964043075
82-83	23.088862447921016	25.091664451106944	26.253332271144416	25.56614082982763
84-85	22.96284012208882	25.080336746729685	26.11783576025032	25.83898737093117
86-87	22.765327571681784	25.155160524917054	26.19149222590838	25.888019677492785
88-89	22.710959992883563	24.898063334809486	26.25666804052293	26.134308631784027
90-91	22.71414282728245	24.799388862129927	26.249782824737828	26.236685485849797
92-93	22.785101489696938	24.69826606518917	26.215603487131283	26.30102895798261
94-95	22.553475999980808	24.599605131477993	26.26787398543689	26.57904488310431
96-97	22.397414847437247	24.438886016189088	26.38611083000242	26.777588306371246
98-99	22.222076485390666	24.371744514661163	26.338285328687384	27.06789367126079
100-101	22.372164347100018	24.2668864843076	26.391798640784774	26.969150527807606
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	61.0
1	53.5
2	63.5
3	105.5
4	110.0
5	111.0
6	194.0
7	375.0
8	572.5
9	620.5
10	527.5
11	473.5
12	504.0
13	552.5
14	624.5
15	747.0
16	843.0
17	922.5
18	1039.0
19	1185.5
20	1361.5
21	1574.5
22	1897.5
23	2336.5
24	2857.5
25	3629.0
26	4720.5
27	6234.0
28	8562.5
29	12281.0
30	17808.0
31	25484.5
32	35438.0
33	44545.0
34	53411.0
35	64264.5
36	77069.0
37	89591.0
38	100758.0
39	111607.5
40	121643.0
41	131690.0
42	142662.0
43	155029.5
44	166775.0
45	177647.0
46	187495.0
47	195851.0
48	204583.0
49	211175.5
50	212713.5
51	211801.0
52	210184.0
53	208805.0
54	203594.0
55	191632.5
56	176983.5
57	159941.0
58	142531.5
59	123884.0
60	103999.0
61	85970.5
62	70006.0
63	54216.0
64	39953.0
65	29806.5
66	22376.0
67	17261.5
68	13312.5
69	10176.5
70	7785.0
71	5966.5
72	4481.0
73	3557.0
74	2835.0
75	2080.0
76	1420.5
77	872.0
78	530.0
79	297.5
80	156.5
81	82.5
82	46.5
83	30.0
84	16.0
85	10.0
86	6.5
87	4.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19552293427716655
2	0.0
3	0.0120069166664534
4	0.013947643871865938
5	0.006376675103498342
6	0.10763571654634158
7	0.14288870633257156
8	0.12392929440277213
9	0.14259013291635425
10-11	0.11369249156103566
12-13	0.11531331867764394
14-15	0.11596378219154593
16-17	0.06614467502842845
18-19	0.07950583540415324
20-21	0.03679917354878391
22-23	0.08385647661189125
24-25	0.05761400599364806
26-27	0.06572880491298291
28-29	0.03959296765767449
30-31	0.024397713439471915
32-33	0.03133954536652446
34-35	0.019066045292734174
36-37	0.023811229943330764
38-39	0.031862048844904754
40-41	0.024749603537156606
42-43	0.01307325029580095
44-45	0.026178490600482322
46-47	0.03341889594375218
48-49	0.009223785893856298
50-51	0.015547144315887263
52-53	0.006621931838248278
54-55	0.012614726835181504
56-57	0.004873144686118299
58-59	0.003508237640553436
60-61	0.022872856349504922
62-63	0.010151496151388665
64-65	0.007325712033617661
66-67	0.03999817443682656
68-69	0.08934809480303112
70-71	0.02152927597652701
72-73	0.044807339105184
74-75	0.08615975725128194
76-77	0.26047331564072573
78-79	0.08073211907790293
80-81	0.016442864564539205
82-83	0.011356453152551394
84-85	0.012241510064909861
86-87	0.27209635220061407
88-89	0.6255219703115656
90-91	0.26533579699055054
92-93	0.3512502975070826
94-95	0.001311590364097485
96-97	0.004020077782640259
98-99	0.0031350208702817938
100-101	0.004883808022411774
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4688964.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.05405934437525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.34198179215323	41.47881936708043
2	12.999117964828697	14.313084237204269
3	4.725528281813769	7.804785433814965
4	2.2229271744203216	4.895246583150432
5	1.1992570052160025	3.301198316715977
6	0.7765647952299847	2.565182659280651
7	0.5093941176614423	1.9630949788386098
8	0.3794372124185534	1.6711647047964282
9	0.27876668354842016	1.3812513785378457
>10	1.4528832142039312	14.306041397811914
>50	0.08621741910987878	3.2226293165160023
>100	0.026447798365842396	2.4098990401134257
>500	0.0010102654251593977	0.35489326616515604
>1k	4.6627560475622285E-4	0.3327093199739723
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.1326672586950976E-5
2	0.0	0.0	0.0	2.1326672586950976E-5	2.1326672586950976E-5
3	0.0	0.0	0.0	2.1326672586950976E-5	2.1326672586950976E-5
4	0.0	0.0	0.0	2.1326672586950976E-5	2.1326672586950976E-5
5	0.0	0.0	0.0	2.1326672586950976E-5	2.1326672586950976E-5
6	0.0	0.0	0.0	2.1326672586950976E-5	2.1326672586950976E-5
7	0.0	0.0	0.0	2.1326672586950976E-5	4.265334517390195E-5
8	0.0	0.0	0.0	2.1326672586950976E-5	4.265334517390195E-5
9	0.0	0.0	0.0	4.265334517390195E-5	4.265334517390195E-5
10-11	0.0	0.0	0.0	6.398001776085294E-5	4.265334517390195E-5
12-13	0.0	0.0	0.0	1.0663336293475489E-4	9.59700266412794E-5
14-15	0.0	0.0	0.0	1.2796003552170587E-4	1.0663336293475489E-4
16-17	0.0	0.0	0.0	1.4928670810865683E-4	1.0663336293475489E-4
18-19	0.0	0.0	0.0	1.706133806956078E-4	1.0663336293475489E-4
20-21	0.0	0.0	0.0	2.1326672586950978E-4	1.0663336293475489E-4
22-23	0.0	0.0	0.0	2.1326672586950978E-4	1.0663336293475489E-4
24-25	0.0	2.1326672586950976E-5	0.0	4.37196788032495E-4	1.0663336293475489E-4
26-27	0.0	5.3316681467377444E-5	0.0	8.530669034780391E-4	1.0663336293475489E-4
28-29	0.0	7.464335405432841E-5	0.0	0.003945434428585931	1.0663336293475489E-4
30-31	0.0	8.53066903478039E-5	0.0	0.01902339194756027	1.0663336293475489E-4
32-33	0.0	8.53066903478039E-5	0.0	0.05522541866390955	1.0663336293475489E-4
34-35	0.0	8.53066903478039E-5	0.0	0.10169623823087573	1.0663336293475489E-4
36-37	0.0	9.59700266412794E-5	0.0	0.17611566222304115	1.1729669922823038E-4
38-39	0.0	1.0663336293475489E-4	0.0	0.30743464867719184	1.2796003552170587E-4
40-41	0.0	1.0663336293475489E-4	0.0	0.4669261696187047	1.2796003552170587E-4
42-43	0.0	1.2796003552170587E-4	0.0	0.6350336662853457	1.2796003552170587E-4
44-45	0.0	1.2796003552170587E-4	0.0	0.836336128833576	1.2796003552170587E-4
46-47	0.0	1.2796003552170587E-4	0.0	1.0724117310348298	1.2796003552170587E-4
48-49	0.0	1.2796003552170587E-4	0.0	1.3283744554234156	1.2796003552170587E-4
50-51	0.0	1.2796003552170587E-4	0.0	1.6141092147433846	1.2796003552170587E-4
52-53	0.0	1.2796003552170587E-4	0.0	1.9121068108008505	1.2796003552170587E-4
54-55	0.0	1.2796003552170587E-4	0.0	2.241443952224841	1.2796003552170587E-4
56-57	0.0	1.4928670810865683E-4	0.0	2.614052912327755	1.2796003552170587E-4
58-59	0.0	1.812767169890833E-4	0.0	3.0178627944253784	1.2796003552170587E-4
60-61	0.0	1.919400532825588E-4	0.0	3.459634153727775	1.2796003552170587E-4
62-63	0.0	1.919400532825588E-4	0.0	3.919000017914405	1.4928670810865683E-4
64-65	0.0	1.919400532825588E-4	0.0	4.398882141129683	1.4928670810865683E-4
66-67	1.0663336293475488E-5	1.919400532825588E-4	0.0	4.945570066223584	1.4928670810865683E-4
68-69	2.1326672586950976E-5	1.919400532825588E-4	0.0	5.541437298302994	1.4928670810865683E-4
70-71	2.1326672586950976E-5	1.919400532825588E-4	0.0	6.177569288226568	1.4928670810865683E-4
72-73	2.1326672586950976E-5	1.919400532825588E-4	0.0	6.842481622806232	1.5995004440213232E-4
74-75	2.1326672586950976E-5	1.919400532825588E-4	0.0	7.52262120161298	1.706133806956078E-4
76-77	2.1326672586950976E-5	1.919400532825588E-4	0.0	8.255810878479767	1.706133806956078E-4
78-79	2.1326672586950976E-5	1.919400532825588E-4	0.0	9.057021977562634	1.706133806956078E-4
80-81	2.1326672586950976E-5	2.0260338957603429E-4	0.0	9.89203585269582	1.706133806956078E-4
82-83	2.1326672586950976E-5	2.1326672586950978E-4	0.0	10.744324332624434	1.706133806956078E-4
84-85	2.1326672586950976E-5	2.3459339845646073E-4	0.0	11.600760850371213	1.706133806956078E-4
86-87	2.1326672586950976E-5	2.3459339845646073E-4	0.0	12.488814160228145	1.919400532825588E-4
88-89	2.1326672586950976E-5	2.3459339845646073E-4	0.0	13.438597523888006	2.1326672586950978E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6660	0.0	43.769207	1
GGTATCA	3680	0.0	41.60931	1
TCAACGC	8460	0.0	33.777935	4
CAACGCA	8775	0.0	32.34487	5
ATCAACG	8930	0.0	32.000153	3
TATCAAC	9375	0.0	30.68145	2
AACGCAG	9360	0.0	30.323639	6
GTACATG	15555	0.0	26.260656	1
TACATGG	16490	0.0	24.581692	2
ACGCAGA	11565	0.0	24.376886	7
ACATGGG	16925	0.0	23.446856	3
CGCAGAG	11930	0.0	23.31007	8
CATGGGG	12615	0.0	20.056122	4
GAGTACA	12200	0.0	19.9179	1
GCAGAGT	13825	0.0	19.01652	9
GTGTACG	555	0.0	18.850035	1
AGTACAT	12075	0.0	18.671577	2
AGAGTAC	11890	0.0	18.337944	10-11
GTGGTAT	2755	0.0	17.778622	1
GTATATA	1655	0.0	17.527271	1
>>END_MODULE