FastQCFastQC Report
Mon 15 Aug 2016
SRR2920848_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920848_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2128070
Sequences flagged as poor quality0
Sequence length98
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35150.042.161421
GGTATCA19450.041.6464271
TCAACGC43150.033.4898644
ATCAACG43650.033.316453
CAACGCA43700.033.2783245
TATCAAC46250.031.8410322
AACGCAG46400.031.1375436
GTACATG66450.028.6741161
TACATGG69450.026.6871972
ACATGGG69750.025.8482993
ACGCAGA56650.025.0114047
CGCAGAG57850.024.4121748
GAGTACA48450.022.1333311
TATAGCG1252.8558305E-422.0203845
CATGGGG44850.020.8665834
GCAGAGT65750.020.8513689
GTATAGA5950.020.1113411
TAAGGTG8800.019.8100035
GTAAGGT6600.019.462464
AGTACAT51850.018.934722
Produced by FastQC (version 0.11.5)