##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920848_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2128070
Sequences flagged as poor quality	0
Sequence length	98
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.5619608377544	31.0	30.0	34.0	26.0	34.0
2	29.636164693830562	31.0	30.0	34.0	25.0	34.0
3	29.71178062751695	31.0	30.0	34.0	25.0	34.0
4	33.28679930641379	35.0	35.0	37.0	28.0	37.0
5	33.257611826678634	35.0	35.0	37.0	27.0	37.0
6	33.377837195205046	36.0	35.0	37.0	28.0	37.0
7	33.24179796717213	35.0	35.0	37.0	27.0	37.0
8	33.19474171432331	35.0	35.0	37.0	27.0	37.0
9	34.587106627131625	38.0	35.0	39.0	27.0	39.0
10-11	34.41505660058175	38.0	34.5	39.0	27.0	39.0
12-13	34.279960010713936	38.0	34.0	39.0	27.0	39.0
14-15	35.15505927906507	38.5	34.5	40.0	26.5	41.0
16-17	35.055306921294886	38.0	34.0	40.0	26.0	41.0
18-19	34.95361219320793	38.5	34.0	40.0	25.0	41.0
20-21	34.87086538506722	38.5	34.0	40.0	25.0	41.0
22-23	34.65908734205172	38.0	34.0	40.0	24.5	41.0
24-25	34.472026766036834	38.0	34.0	40.0	24.0	41.0
26-27	34.542307348912395	38.0	34.0	40.0	24.0	41.0
28-29	34.408102646999396	38.0	34.0	40.0	23.5	41.0
30-31	34.17946496120898	38.0	33.5	40.0	22.0	41.0
32-33	33.94845799245326	38.0	33.0	40.0	20.0	41.0
34-35	33.63472794597922	38.0	33.0	40.0	17.5	41.0
36-37	33.34306249324506	38.0	32.5	40.0	16.0	41.0
38-39	33.14985315332672	38.0	32.0	40.0	15.0	41.0
40-41	32.958766393962605	38.0	32.0	40.0	14.0	41.0
42-43	32.73339692773264	37.5	31.5	40.0	9.5	41.0
44-45	32.46198268853938	37.0	31.0	40.0	9.0	41.0
46-47	32.20841349203739	37.0	31.0	40.0	8.0	41.0
48-49	31.904214147091025	37.0	30.5	40.0	7.0	41.0
50-51	30.760580009116243	35.5	29.0	38.0	4.0	40.0
52-53	31.24506336727645	35.5	30.5	38.5	2.0	39.5
54-55	31.914493414220395	37.0	31.0	39.5	2.0	40.5
56-57	31.872820442936558	37.0	31.0	40.0	2.0	41.0
58-59	31.633519339119484	36.0	31.0	39.5	2.0	41.0
60-61	31.309424971922915	36.0	30.0	39.0	2.0	41.0
62-63	30.926962928851026	35.0	30.0	39.0	2.0	40.0
64-65	30.50456328974141	35.0	29.0	39.0	2.0	40.0
66-67	30.11827219029449	35.0	29.0	38.0	2.0	40.0
68-69	29.668827388196817	34.5	28.0	37.5	2.0	40.0
70-71	29.343053095057964	34.0	28.0	37.0	2.0	39.5
72-73	28.884422504898808	34.0	28.0	36.5	2.0	39.0
74-75	28.439837975254573	34.0	27.0	36.0	2.0	39.0
76-77	28.02437701767329	34.0	26.0	35.5	2.0	38.0
78-79	27.567351637869056	33.0	26.0	35.0	2.0	37.0
80-81	27.123027907916565	33.0	25.5	35.0	2.0	37.0
82-83	26.65296982712035	33.0	25.0	35.0	2.0	36.0
84-85	26.26900125465798	33.0	24.0	35.0	2.0	36.0
86-87	25.826436630374	32.0	22.5	35.0	2.0	35.0
88-89	25.300214983529678	32.0	20.0	34.5	2.0	35.0
90-91	24.81356463838126	32.0	18.0	34.0	2.0	35.0
92-93	24.283959879139317	31.0	10.0	34.0	2.0	35.0
94-95	23.650058973623988	31.0	2.0	34.0	2.0	35.0
96-97	23.007240363333914	31.0	2.0	34.0	2.0	35.0
98	22.611228953934788	31.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	76673.0
3	12896.0
4	9491.0
5	7712.0
6	7279.0
7	7410.0
8	8577.0
9	9895.0
10	10753.0
11	12026.0
12	13043.0
13	13535.0
14	12681.0
15	13265.0
16	15050.0
17	16326.0
18	16672.0
19	15888.0
20	16663.0
21	17448.0
22	19281.0
23	21544.0
24	24766.0
25	28396.0
26	33435.0
27	39615.0
28	46744.0
29	55615.0
30	66264.0
31	80234.0
32	98874.0
33	124339.0
34	159534.0
35	210325.0
36	276247.0
37	314071.0
38	193958.0
39	21544.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.17114644635582	17.946799079523164	12.593886751169459	25.28816772295156
2	16.07712749706946	19.31110916838457	39.19972513552868	25.412038199017296
3	19.967147701396577	23.031012287887652	28.545122561633306	28.456717449082465
4	13.294917220184862	15.061804636158428	36.08722440997616	35.55605373368056
5	14.384073178102783	36.72328519372015	34.16659185732864	14.726049770848423
6	34.22574004267759	34.52890138091165	16.80331644403501	14.442042132375752
7	29.813197255764855	31.194197215347643	20.486078148101846	18.506527380785652
8	27.173522788365016	34.178506039678034	19.680741077705637	18.96723009425131
9	26.855648545395596	15.655218108426885	19.325163176023345	38.16397017015418
10-11	25.84010394394921	25.133031338254852	27.377177442471346	21.649687275324588
12-13	26.317214103918463	22.453895156712296	27.409355180697524	23.819535558671713
14-15	23.39785890119898	24.128867240173317	24.729919063241667	27.74335479538604
16-17	23.50664092393747	27.32044646106788	25.580674061944674	23.59223855304997
18-19	23.468148531520537	26.240064080642327	27.283795405438628	23.007991982398508
20-21	23.937513299232435	25.64082608659872	27.11538619795371	23.306274416215132
22-23	23.769881332260027	25.49248135065011	26.891655866611263	23.8459814504786
24-25	23.491697344320944	25.870601618990236	26.890833942419658	23.746867094269167
26-27	23.340665779186796	26.040653224058257	26.87561629458187	23.743064702173072
28-29	23.213790791579797	26.1956115207864	26.965632332223215	23.624965355410584
30-31	23.575374765907203	25.97552671026134	26.74650797131956	23.70259055251189
32-33	23.243731009735704	26.01425929450801	26.773329385473282	23.968680310283
34-35	23.70875039859384	26.06854376377697	26.63655618694234	23.586149650686856
36-37	23.790152476068865	25.730115304287597	26.581175819031543	23.89855640061199
38-39	23.452717999445937	25.999543157677145	26.721571122279414	23.826167720597503
40-41	23.58571761999981	25.88908521129071	26.874014274450538	23.65118289425895
42-43	23.59979865198732	25.81502778913938	26.597091209989276	23.988082348884024
44-45	23.472555978728675	25.919088775955384	26.714735464396465	23.89361978091948
46-47	23.699630114809437	25.88670453165366	26.472295162958186	23.941370190578716
48-49	23.467378155897258	26.08294996073919	26.3747061334113	24.074965749952252
50-51	23.41695115400704	26.05333412560641	26.574347989913612	23.955366730472942
52-53	23.56446379477244	25.939178114898574	26.642528039507635	23.85383005082135
54-55	23.273533290708894	25.893662249296344	26.816072401777014	24.016732058217748
56-57	23.187099330625646	26.02101995858276	26.644920255296267	24.146960455495332
58-59	23.22643345343337	25.68556776155953	26.816864818361864	24.271133966645237
60-61	23.12075686731802	25.784047896689273	26.986465373773054	24.108729862219654
62-63	23.07293047193135	25.672557122549716	27.09617566852461	24.158336736994322
64-65	23.033172112775745	25.613684086351302	26.962180016515276	24.390963784357673
66-67	23.043508732683122	25.75679596082876	26.793159781683514	24.406535524804603
68-69	23.205640292222004	25.441196170623844	26.907258947034762	24.445904590119394
70-71	22.98039885037771	25.41791212617649	26.95822305039369	24.643465973052102
72-73	23.11558321983342	25.351001132877638	26.796846234607973	24.73656941268097
74-75	22.938867646337926	25.6507338107251	26.808484262286424	24.60191428065055
76-77	22.947093301407214	25.38746161442143	27.004734712346785	24.660710371824575
78-79	22.9951823572748	25.32571290129936	26.901286165903947	24.777818575521888
80-81	23.084939078028963	25.322311782081975	26.85358738623389	24.739161753655175
82-83	23.065814241045313	25.298104622642796	26.89984391786431	24.73623721844758
84-85	22.86966449761379	25.31135745249736	26.87479140700571	24.94418664288314
86-87	22.950743175742698	25.2465763529442	26.944041643953902	24.8586388273592
88-89	22.961579103966667	25.034245008952034	26.844644835051046	25.159531052030253
90-91	22.97466504492782	25.114818167246533	26.903515967527508	25.007000820298135
92-93	23.187123138569987	24.93404667183467	26.887556793634733	24.991273395960615
94-95	22.954484384207426	24.70781378903948	27.13423688862699	25.203464938126103
96-97	23.15762850813696	24.51039412194808	27.03320263976802	25.29877473014694
98	23.170510379509583	24.497452792876963	26.854025963740895	25.47801086387256
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	110.0
1	78.5
2	79.5
3	136.5
4	130.0
5	126.5
6	234.0
7	449.0
8	673.0
9	787.5
10	782.0
11	775.0
12	839.0
13	914.0
14	1018.0
15	1130.5
16	1236.5
17	1303.0
18	1323.0
19	1396.5
20	1460.0
21	1533.0
22	1767.0
23	2134.0
24	2609.0
25	3427.0
26	4620.0
27	6265.5
28	8218.0
29	10890.5
30	14766.5
31	20579.5
32	25928.5
33	30133.0
34	35773.5
35	41630.5
36	47140.5
37	51870.5
38	54774.0
39	57874.5
40	61653.5
41	64207.0
42	67537.5
43	73265.0
44	78800.0
45	82150.5
46	84428.0
47	86921.0
48	88531.5
49	88843.5
50	88889.0
51	89303.5
52	89886.5
53	89989.0
54	87285.5
55	81996.0
56	75368.5
57	68346.0
58	62571.5
59	55151.5
60	46431.5
61	38294.5
62	31129.5
63	24008.5
64	17614.0
65	13491.0
66	10315.0
67	8083.0
68	6331.5
69	4851.5
70	3706.0
71	2778.0
72	2146.0
73	1695.5
74	1334.5
75	976.5
76	680.0
77	437.5
78	246.5
79	153.0
80	94.5
81	49.5
82	33.0
83	22.5
84	14.5
85	11.5
86	10.0
87	7.0
88	5.5
89	7.5
90	5.0
91	1.5
92	2.0
93	1.5
94	0.5
95	1.0
96	1.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.34914265038274117
2	0.02236768527351074
3	0.017151691438721473
4	0.014896126537190975
5	0.020629020661914316
6	0.023025558369790467
7	0.01240560695841772
8	0.00667271283369438
9	0.0
10-11	0.0
12-13	0.004417147932163886
14-15	0.0023730422401518746
16-17	0.004252679658093953
18-19	0.035266697054138256
20-21	0.07224856325214866
22-23	0.09038706433528972
24-25	0.05876216477841425
26-27	0.06808986546495181
28-29	0.05237139755741117
30-31	0.04583965753006245
32-33	0.01574196337526491
34-35	0.3077671317202912
36-37	0.47298726075739994
38-39	0.43123581461136146
40-41	0.4412213883941788
42-43	0.39270324754354885
44-45	0.36864388859389025
46-47	0.36735163786905506
48-49	0.35877579214969435
50-51	0.35661420911906094
52-53	0.4127448815123563
54-55	0.4128153679155291
56-57	0.38438585196915515
58-59	0.37089945349542075
60-61	0.38410390635646385
62-63	0.3667172602404996
64-65	0.41446005065622843
66-67	0.43323292936792496
68-69	0.37771313913546073
70-71	0.33831123976184996
72-73	0.32531824611032534
74-75	0.30741469970442703
76-77	0.3056760350928306
78-79	0.30511214386744795
80-81	0.3210420709845071
82-83	0.3172358052131744
84-85	0.31549714060157796
86-87	0.30381989314261276
88-89	0.3441146202897461
90-91	0.3237675452405231
92-93	0.3140639170703971
94-95	0.32047817975912446
96-97	0.32061915256547013
98	0.3161080227624091
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	2128070.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.98117608716305
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.38775678586823	33.7728735272315
2	13.097667409166549	12.568829725806339
3	5.902465946478353	8.49621773779384
4	3.189211578511068	6.120884893110352
5	2.010959646785003	4.824410445828523
6	1.2529748082451635	3.607152294431433
7	0.904232397001945	3.03702937049875
8	0.6237777410596518	2.3943671706428757
9	0.4605932951861096	1.9889827200802255
>10	2.0473496505396747	16.455134528309348
>50	0.0836188684381278	2.740726219421076
>100	0.0376158959835412	3.168667168858002
>500	0.0010853191167607993	0.39236060805482387
>1k	6.906576197568636E-4	0.43236358993299656
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.6990935448551976E-5
2	0.0	0.0	0.0	0.0	4.6990935448551976E-5
3	0.0	0.0	0.0	0.0	4.6990935448551976E-5
4	0.0	9.398187089710395E-5	0.0	0.0	4.6990935448551976E-5
5	0.0	9.398187089710395E-5	0.0	0.0	4.6990935448551976E-5
6	0.0	9.398187089710395E-5	0.0	0.0	2.8194561269131183E-4
7	0.0	9.398187089710395E-5	0.0	0.0	2.8194561269131183E-4
8	0.0	9.398187089710395E-5	0.0	0.0	2.8194561269131183E-4
9	0.0	9.398187089710395E-5	0.0	0.0	2.8194561269131183E-4
10-11	0.0	9.398187089710395E-5	0.0	0.0	5.638912253826237E-4
12-13	0.0	9.398187089710395E-5	0.0	0.0	0.0011042869830409714
14-15	0.0	9.398187089710395E-5	0.0	4.6990935448551976E-5	0.0013392416602837312
16-17	0.0	9.398187089710395E-5	0.0	4.6990935448551976E-5	0.0015507008698022152
18-19	0.0	9.398187089710395E-5	0.0	4.6990935448551976E-5	0.001691673676147871
20-21	0.0	9.398187089710395E-5	0.0	4.6990935448551976E-5	0.001903132885666355
22-23	0.0	1.4097280634565591E-4	0.0	2.819456126913119E-4	0.001973619288839183
24-25	0.0	1.4097280634565591E-4	0.0	5.638912253826237E-4	0.001973619288839183
26-27	0.0	1.4097280634565591E-4	0.0	0.0013157461925594554	0.002020610224287735
28-29	0.0	1.4097280634565591E-4	0.0	0.004746084480303749	0.002020610224287735
30-31	0.0	1.879637417942079E-4	0.0	0.021028443613227008	0.002067601159736287
32-33	0.0	2.8194561269131183E-4	0.0	0.05495589900708153	0.002114592095184839
34-35	0.0	2.8194561269131183E-4	0.0	0.09875145084513198	0.0021615830306333907
36-37	0.0	3.2893654813986384E-4	0.0	0.17027165459782806	0.0021615830306333907
38-39	0.0	3.759274835884158E-4	0.0	0.2913437997810222	0.0023260513047033225
40-41	0.0	3.759274835884158E-4	0.0	0.43748560902601885	0.0023495467724275985
42-43	0.0	4.2291841903696777E-4	0.0	0.5892193395893932	0.0023965377078761507
44-45	0.0	4.6990935448551973E-4	0.0	0.7757498578524202	0.0025140150464975303
46-47	0.0	4.6990935448551973E-4	0.0	1.0066398191788803	0.002654987852843187
48-49	0.0	5.638912253826237E-4	0.0	1.2583232694413247	0.0027724651914645664
50-51	0.0	5.638912253826237E-4	0.0	1.5428768790500311	0.0028664470623616703
52-53	0.0	5.638912253826237E-4	0.0	1.8266316427561122	0.0029604289332587743
54-55	0.0	5.638912253826237E-4	0.0	2.1365603575070367	0.0031014017396044304
56-57	0.0	5.638912253826237E-4	0.0	2.510702185548408	0.003171888142777258
58-59	0.0	5.638912253826237E-4	0.0	2.9260550639781586	0.003265870013674362
60-61	0.0	5.638912253826237E-4	0.0	3.368451225758551	0.0032893654813986382
62-63	0.0	5.638912253826237E-4	0.0	3.818788855629749	0.0034303382877442943
64-65	0.0	6.108821608311756E-4	0.0	4.2958408323034485	0.0034538337554685704
66-67	0.0	6.108821608311756E-4	0.0	4.854727523060802	0.003477329223192846
68-69	0.0	6.108821608311756E-4	0.0	5.481586601944485	0.0035243201586413983
70-71	0.0	6.108821608311756E-4	0.0	6.15137190036042	0.003594806561814226
72-73	0.0	6.108821608311756E-4	0.0	6.824658023467274	0.00368878843271133
74-75	0.0	6.108821608311756E-4	0.0	7.5036065542956765	0.00380626577133271
76-77	0.0	6.108821608311756E-4	0.0	8.24002499917766	0.0038532567067812618
78-79	0.0	6.108821608311756E-4	0.0	9.064645429896572	0.0038532567067812618
80-81	0.0	6.108821608311756E-4	0.0	9.932450530292707	0.0039002476422298135
82-83	0.0	6.108821608311756E-4	0.0	10.809160412956341	0.003994229513126918
84-85	0.0	6.578730962797277E-4	0.0	11.699333198625984	0.00404122044857547
86	0.0	6.578730962797277E-4	0.0	12.375908687214237	0.004088211384024021
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3515	0.0	42.16142	1
GGTATCA	1945	0.0	41.646427	1
TCAACGC	4315	0.0	33.489864	4
ATCAACG	4365	0.0	33.31645	3
CAACGCA	4370	0.0	33.278324	5
TATCAAC	4625	0.0	31.841032	2
AACGCAG	4640	0.0	31.137543	6
GTACATG	6645	0.0	28.674116	1
TACATGG	6945	0.0	26.687197	2
ACATGGG	6975	0.0	25.848299	3
ACGCAGA	5665	0.0	25.011404	7
CGCAGAG	5785	0.0	24.412174	8
GAGTACA	4845	0.0	22.133331	1
TATAGCG	125	2.8558305E-4	22.020384	5
CATGGGG	4485	0.0	20.866583	4
GCAGAGT	6575	0.0	20.851368	9
GTATAGA	595	0.0	20.111341	1
TAAGGTG	880	0.0	19.810003	5
GTAAGGT	660	0.0	19.46246	4
AGTACAT	5185	0.0	18.93472	2
>>END_MODULE