FastQCFastQC Report
Mon 15 Aug 2016
SRR2920848_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920848_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2128070
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20850.050.6233831
GTATCAA39250.043.2446941
TCAACGC51300.031.9257564
ATCAACG51800.031.8925253
TATCAAC53050.031.4087722
CAACGCA52150.030.9444165
AACGCAG56350.028.553776
GTACATG70000.025.0629731
ACGCAGA67750.023.6790947
GGACCGT3050.023.3425946
TACATGG73850.023.2695122
CGCAGAG68950.022.9211838
ACATGGG76300.021.589593
TAGGACA9300.020.4181564
GCAGAGT74950.020.389729
GAGTACA53150.019.8588431
GTATAGT3003.6779966E-919.0179751
TAAGGTG9150.018.6740745
GAGTACT34050.018.60610412-13
AGTACAT54900.018.5906832
Produced by FastQC (version 0.11.5)