##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920848_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2128070
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.92247529451569	31.0	29.0	31.0	25.0	34.0
2	28.939335642154628	31.0	28.0	33.0	23.0	34.0
3	29.067505768137327	31.0	28.0	34.0	25.0	34.0
4	32.65591874327442	35.0	32.0	37.0	26.0	37.0
5	32.0186055909815	35.0	32.0	37.0	22.0	37.0
6	31.933422772747136	35.0	32.0	37.0	22.0	37.0
7	31.722659498982647	35.0	32.0	37.0	19.0	37.0
8	31.61359165816914	35.0	32.0	37.0	18.0	37.0
9	32.798148087233976	37.0	32.0	39.0	17.0	39.0
10-11	32.52373794095119	37.0	32.0	39.0	17.0	39.0
12-13	32.300262914283834	37.0	32.0	39.0	16.5	39.0
14-15	32.97995625143909	38.0	32.0	40.0	16.0	41.0
16-17	32.79081585662126	37.5	31.5	40.0	12.0	41.0
18-19	32.666823694709294	37.5	31.0	40.0	10.5	41.0
20-21	32.5205301047428	37.0	31.0	40.0	10.0	41.0
22-23	32.330262162428866	37.0	31.0	40.0	10.0	41.0
24-25	32.085154858627774	37.0	30.5	40.0	10.0	41.0
26-27	31.83709370462438	37.0	30.0	40.0	8.5	41.0
28-29	31.729186774871128	37.0	30.0	40.0	8.0	41.0
30-31	31.674497784377394	37.0	30.0	40.0	8.0	41.0
32-33	31.470446460877696	36.5	30.0	40.0	4.0	41.0
34-35	31.240999591178863	36.0	30.0	39.5	2.0	41.0
36-37	30.985838576738548	36.0	29.5	39.0	2.0	40.0
38-39	30.74425065904787	36.0	29.0	39.0	2.0	40.0
40-41	30.454134732410118	35.5	28.0	39.0	2.0	40.0
42-43	30.414055693656692	36.0	28.0	39.0	2.0	40.0
44-45	30.194129187479735	35.0	28.0	39.0	2.0	40.0
46-47	29.86372299783372	35.0	27.0	39.0	2.0	40.0
48-49	29.510250602658747	35.0	26.5	39.0	2.0	40.0
50-51	29.166155953516565	35.0	26.0	39.0	2.0	40.0
52-53	28.83443660217944	34.5	25.0	38.5	2.0	40.0
54-55	28.486379677360237	34.0	24.0	38.0	2.0	40.0
56-57	28.09486929471305	34.0	23.5	38.0	2.0	40.0
58-59	27.689880502051153	33.5	23.0	38.0	2.0	40.0
60-61	27.2934915204857	33.0	22.0	38.0	2.0	40.0
62-63	26.924315694502532	33.0	21.5	37.0	2.0	39.5
64-65	26.459123055162657	32.5	19.5	37.0	2.0	39.0
66-67	25.958493846537003	32.0	18.0	36.0	2.0	39.0
68-69	25.47354457325182	31.5	16.0	36.0	2.0	39.0
70-71	24.990349236632255	31.0	12.5	35.0	2.0	38.0
72-73	24.50460910590347	31.0	8.0	35.0	2.0	37.5
74-75	24.02117364560376	30.0	7.5	34.5	2.0	37.0
76-77	22.94705084889125	29.0	7.0	33.5	2.0	35.5
78-79	23.7097297081393	30.5	6.5	34.0	2.0	36.0
80-81	23.6953384992035	31.0	2.0	34.0	2.0	36.0
82-83	23.30265710244494	30.0	2.0	34.0	2.0	35.5
84-85	22.915481398638203	30.0	2.0	34.0	2.0	35.0
86-87	22.29981438580498	29.5	2.0	34.0	2.0	35.0
88-89	21.851146108915593	29.0	2.0	34.0	2.0	35.0
90-91	21.37605764847961	29.0	2.0	34.0	2.0	35.0
92-93	20.9436959310549	29.0	2.0	34.0	2.0	35.0
94-95	20.416368117590117	28.5	2.0	34.0	2.0	35.0
96-97	19.7062514390974	27.0	2.0	33.5	2.0	35.0
98-99	18.906667778785472	26.0	2.0	33.0	2.0	35.0
100-101	16.773112491600372	21.0	2.0	31.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	100161.0
3	38019.0
4	20079.0
5	14061.0
6	11921.0
7	12034.0
8	13183.0
9	15301.0
10	17150.0
11	18981.0
12	20961.0
13	22683.0
14	24213.0
15	25681.0
16	26986.0
17	27562.0
18	26063.0
19	25125.0
20	24897.0
21	25907.0
22	27371.0
23	30939.0
24	35117.0
25	40718.0
26	47166.0
27	55268.0
28	64074.0
29	74374.0
30	85848.0
31	99433.0
32	115457.0
33	137048.0
34	163069.0
35	193085.0
36	213749.0
37	175517.0
38	57071.0
39	1798.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.61720223194826	20.677388955678296	13.665834754111941	24.039574058261497
2	17.053433392698548	21.672501374484863	36.71932784165934	24.554737391157246
3	20.513379649885962	24.819192967829533	29.06583777488802	25.601589607396484
4	14.914748894736421	17.11376166536562	35.05811005215312	32.91337938774484
5	14.397606327986075	37.412356716154186	34.01917531664367	14.17086163921607
6	34.02259078578548	35.66212320271168	16.288311222812244	14.026974788690588
7	29.48152449284582	31.649610952798618	20.46991518883941	18.398949365516152
8	27.120178248154854	34.902021851959184	19.464512207514577	18.513287692371385
9	26.80589963838953	15.587552318236037	19.184082551647716	38.42246549172672
10-11	26.02524301120085	25.587826377796535	27.19175792939662	21.195172681605992
12-13	26.52501570023027	22.71149445023321	27.331878170917296	23.431611678619227
14-15	23.478710842863393	24.396089813852182	24.64042940542778	27.48476993785664
16-17	23.637293347627377	27.574597039628358	25.471538866632816	23.316570746111456
18-19	23.590716414625682	26.384642225321723	27.304366881635882	22.72027447841671
20-21	24.175928263229125	25.7006542934275	27.047199474647364	23.076217968696014
22-23	23.95142657340684	25.65828735946245	26.89406783048633	23.496218236644374
24-25	23.737605491431392	26.0102259103411	26.882413784997294	23.369754813230212
26-27	23.533820551268146	26.141249942988637	26.92337531720652	23.401554188536696
28-29	23.477525701181154	26.19845808190767	27.026814219341972	23.297201997569204
30-31	23.701411932806288	26.09455343629852	26.821788134876247	23.38224649601895
32-33	23.529714530676248	26.087925228584158	26.754734789728897	23.627625451010697
34-35	23.92684488118145	26.107699494947123	26.68212312085644	23.283332503014993
36-37	23.942237031873834	25.810805316716106	26.666679200401017	23.58027845100904
38-39	23.72691063584959	25.998307334234738	26.743296495956244	23.531485533959433
40-41	23.768966344645477	25.885248841214892	26.92226251632452	23.423522297815115
42-43	23.814756077988687	25.857808091515196	26.622604386693148	23.70483144380297
44-45	23.647184900906915	25.91026276765004	26.73661426667826	23.705938064764787
46-47	23.852792273065603	25.937951472103123	26.476915299338565	23.73234095549271
48-49	23.607118847897258	26.105919896160557	26.38389341982861	23.90306783611358
50-51	23.65319744660152	26.085035736064228	26.478878534979984	23.782888282354268
52-53	23.875475373396476	25.90629636983316	26.577064827009316	23.641163429761047
54-55	23.60231268747114	25.85406946756727	26.62854484209834	23.91507300286325
56-57	23.448849176670887	26.02744908132209	26.432458253180897	24.091243488826123
58-59	23.41697127563115	25.776274123866923	26.655220872729647	24.15153372777228
60-61	23.448204388728875	25.896983124901297	26.69752099987216	23.957291486497663
62-63	23.299349299907608	25.88563074109129	26.755353495668423	24.05966646333268
64-65	23.15728480283506	25.79076391971245	26.60615862436978	24.445792653082705
66-67	23.211636867567805	25.92915297445298	26.467847223217888	24.391362934761325
68-69	23.361868315493876	25.629656315093175	26.58315301049392	24.425322358919033
70-71	23.17009520089808	25.546651401058956	26.56934841752095	24.713904980522013
72-73	23.308251236534915	25.46489787466191	26.405682172625283	24.821168716177894
74-75	23.06919953319408	25.80318388348569	26.313326659974496	24.81428992334573
76-77	23.062266780390782	25.541229623970985	26.732106701606533	24.664396894031697
78-79	23.101581242633003	25.490010277642117	26.560639146600657	24.847769333124223
80-81	23.111668922953427	25.561759006015016	26.46536249297089	24.861209578060667
82-83	23.111244579604794	25.58727312118422	26.449155318320006	24.852326980890975
84-85	22.863091209865402	25.528399691706145	26.418833182946088	25.18967591548237
86-87	22.936185606927758	25.44511427088309	26.479435574661153	25.139264547528
88-89	22.88427330838875	25.382694086016045	26.357144595798243	25.37588800979697
90-91	22.77535937793328	25.440488088337165	26.382341046119606	25.401811487609944
92-93	22.910691255168214	25.309255907008826	26.384376185309087	25.395676652513878
94-95	22.66839031280794	25.164928218220744	26.54457349861164	25.622107970359675
96-97	22.80810373967243	24.95267226367911	26.52904949082005	25.710174505828416
98-99	22.64502765027199	25.08559648271999	26.350488344028783	25.91888752297924
100-101	22.94751781048516	24.729670670501324	26.493684091806237	25.829127427207276
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	105.0
1	70.0
2	61.5
3	102.0
4	94.5
5	88.0
6	165.5
7	328.5
8	515.5
9	581.0
10	486.5
11	412.0
12	426.0
13	434.5
14	455.5
15	501.0
16	582.0
17	659.5
18	716.5
19	816.0
20	965.0
21	1146.0
22	1363.5
23	1728.0
24	2181.5
25	2759.5
26	3823.5
27	5284.5
28	6940.0
29	9238.5
30	12387.0
31	16667.5
32	22516.0
33	27306.5
34	31355.5
35	36272.5
36	42138.5
37	48034.5
38	52068.5
39	54810.0
40	57612.5
41	61448.0
42	64912.5
43	68516.0
44	72779.5
45	77636.5
46	81646.5
47	83728.0
48	86049.5
49	87870.5
50	88196.5
51	88351.5
52	88697.5
53	88261.0
54	85547.0
55	80528.5
56	74228.0
57	67595.0
58	61602.5
59	54514.0
60	46364.0
61	38857.0
62	31751.0
63	24836.0
64	18831.5
65	14473.5
66	11074.5
67	8512.0
68	6714.0
69	5122.5
70	3913.0
71	3020.5
72	2311.0
73	1801.0
74	1372.0
75	1009.0
76	707.0
77	452.5
78	276.5
79	171.5
80	101.0
81	63.0
82	37.5
83	21.0
84	10.5
85	2.5
86	1.0
87	1.5
88	3.5
89	2.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1714229325163176
2	0.0
3	0.01170074292668944
4	0.013956307828219936
5	0.0059208578665175485
6	0.10145342963342371
7	0.13523991222093257
8	0.11719539300868863
9	0.1341591207056159
10-11	0.10777371045125395
12-13	0.1083141062089123
14-15	0.10995878894961163
16-17	0.06155812543760309
18-19	0.07349382304153529
20-21	0.03463231942558281
22-23	0.07899176248901588
24-25	0.05262984770237822
26-27	0.061135207018566125
28-29	0.03686438885938902
30-31	0.023025558369790467
32-33	0.029416325590793536
34-35	0.017480627986861334
36-37	0.02248516261213212
38-39	0.03084954912197437
40-41	0.0243648000300742
42-43	0.011677247458965166
44-45	0.024670241110489786
46-47	0.030755567251077264
48-49	0.00796496355852956
50-51	0.014919622004915253
52-53	0.006461253624175896
54-55	0.011395301846273853
56-57	0.00439365246443961
58-59	0.00321887907822581
60-61	0.020582029726465764
62-63	0.009774114573298812
64-65	0.006743199236867208
66-67	0.03742828008477165
68-69	0.08416076538835658
70-71	0.019336769937079137
72-73	0.04020074527623621
74-75	0.08009604947205684
76-77	0.22569746295939513
78-79	0.07563191060444441
80-81	0.014637676392223941
82-83	0.0106669423468213
84-85	0.010807915153166954
86-87	0.24115748072196874
88-89	0.5786228836457448
90-91	0.25090809982754325
92-93	0.33135658131546425
94-95	0.0010807915153166953
96-97	0.004205688722645402
98-99	0.0029134379978102225
100-101	0.005169002899340717
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2128070.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.12765397288344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.75441028412814	39.715264425221186
2	11.50281957664592	12.22235636361108
3	5.063329999395612	8.070085324752306
4	2.703794640825142	5.745850643659792
5	1.6830565467929741	4.470842291740661
6	1.042695743755954	3.323758720395879
7	0.6879423618896723	2.5584134629033866
8	0.5056227049648642	2.149003848816531
9	0.37611027743292985	1.7983671007591697
>10	1.5817120820783113	14.096924106313566
>50	0.06828348476874793	2.458491138465764
>100	0.028351630033602238	2.560169565445789
>500	0.001603429104151087	0.6243218555056712
>1k	2.672381840251738E-4	0.2061511524092367
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.879637417942079E-4
2	0.0	0.0	0.0	0.0	1.879637417942079E-4
3	0.0	0.0	0.0	0.0	1.879637417942079E-4
4	0.0	0.0	0.0	0.0	1.879637417942079E-4
5	0.0	4.6990935448551976E-5	0.0	0.0	2.8194561269131183E-4
6	0.0	4.6990935448551976E-5	0.0	0.0	3.2893654813986384E-4
7	0.0	4.6990935448551976E-5	0.0	0.0	3.2893654813986384E-4
8	0.0	4.6990935448551976E-5	0.0	0.0	3.2893654813986384E-4
9	0.0	4.6990935448551976E-5	0.0	0.0	3.2893654813986384E-4
10-11	0.0	4.6990935448551976E-5	0.0	0.0	4.2291841903696777E-4
12-13	0.0	4.6990935448551976E-5	0.0	0.0	6.343776285554516E-4
14-15	0.0	9.398187089710395E-5	0.0	4.6990935448551976E-5	8.458368380739355E-4
16-17	0.0	9.398187089710395E-5	0.0	4.6990935448551976E-5	0.0010807915153166953
18-19	0.0	9.398187089710395E-5	0.0	4.6990935448551976E-5	0.0012217643216623512
20-21	0.0	9.398187089710395E-5	0.0	4.6990935448551976E-5	0.0013862325957322832
22-23	0.0	9.398187089710395E-5	0.0	1.879637417942079E-4	0.0015037099343536632
24-25	0.0	1.4097280634565594E-4	0.0	4.934048222097957E-4	0.0015507008698022152
26-27	0.0	1.879637417942079E-4	0.0	0.0010103051121438675	0.001691673676147871
28-29	0.0	1.879637417942079E-4	0.0	0.00415869778719685	0.001691673676147871
30-31	0.0	1.879637417942079E-4	0.0	0.01867889684079941	0.001762160079320699
32-33	0.0	2.1145920951848388E-4	0.0	0.04877659099559695	0.0019266283533906309
34-35	0.0	2.3495467724275987E-4	0.0	0.08770858101472226	0.002020610224287735
36-37	0.0	2.3495467724275987E-4	0.0	0.15185120790199572	0.002020610224287735
38-39	0.0	2.8194561269131183E-4	0.0	0.2609876554812577	0.002020610224287735
40-41	0.0	2.8194561269131183E-4	0.0	0.3939250118652112	0.002020610224287735
42-43	0.0	3.0544108041558786E-4	0.0	0.5315144708585714	0.002044105692012011
44-45	0.0	3.2893654813986384E-4	0.0	0.6999769744416302	0.002091096627460563
46-47	0.0	3.2893654813986384E-4	0.0	0.911177733815147	0.0021615830306333907
48-49	0.0	3.524320158641398E-4	0.0	1.1396946529014553	0.002208573966081943
50-51	0.0	3.759274835884158E-4	0.0	1.3957012692251665	0.002302555836979047
52-53	0.0	4.4641388676124375E-4	0.0	1.6516608946134292	0.0024435286433247025
54-55	0.0	5.169002899340717E-4	0.0	1.9301761690169967	0.002537510514221807
56-57	0.0	5.403957576583477E-4	0.0	2.267618076473048	0.0025610059819460825
58-59	0.0	6.578730962797277E-4	0.0	2.643287109916497	0.0026314923851189108
60-61	0.0	6.578730962797277E-4	0.0	3.04073644194035	0.0026314923851189108
62-63	0.0	6.578730962797277E-4	0.0	3.4423679672191234	0.0027724651914645664
64-65	0.0	6.578730962797277E-4	0.0	3.870055966204119	0.0027959606591888425
66-67	0.0	6.813685640040037E-4	0.0	4.371825174923757	0.0028429515946373947
68-69	0.0	7.048640317282797E-4	0.0	4.929889524310761	0.0028664470623616703
70-71	0.0	7.518549671768316E-4	0.0	5.526956350120061	0.0028899425300859464
72-73	0.0	7.988459026253836E-4	0.0	6.13043743861809	0.0030544108041558786
74-75	0.0	7.988459026253836E-4	0.0	6.745877720187776	0.0031953836105015343
76-77	0.0	7.988459026253836E-4	0.0	7.411433834413342	0.0031953836105015343
78-79	0.0	7.988459026253836E-4	0.0	8.154994901483503	0.003312860949122914
80-81	0.0	7.988459026253836E-4	0.0	8.9396260461357	0.003383347352295742
82-83	2.3495467724275988E-5	7.988459026253836E-4	0.0	9.725854882593147	0.00357131109408995
84-85	4.6990935448551976E-5	7.988459026253836E-4	0.0	10.523573002767765	0.00357131109408995
86-87	4.6990935448551976E-5	7.988459026253836E-4	0.0	11.347441578519504	0.003759274835884158
88-89	4.6990935448551976E-5	7.988459026253836E-4	0.0	12.225796143923837	0.003947238577678366
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2085	0.0	50.623383	1
GTATCAA	3925	0.0	43.244694	1
TCAACGC	5130	0.0	31.925756	4
ATCAACG	5180	0.0	31.892525	3
TATCAAC	5305	0.0	31.408772	2
CAACGCA	5215	0.0	30.944416	5
AACGCAG	5635	0.0	28.55377	6
GTACATG	7000	0.0	25.062973	1
ACGCAGA	6775	0.0	23.679094	7
GGACCGT	305	0.0	23.342594	6
TACATGG	7385	0.0	23.269512	2
CGCAGAG	6895	0.0	22.921183	8
ACATGGG	7630	0.0	21.58959	3
TAGGACA	930	0.0	20.418156	4
GCAGAGT	7495	0.0	20.38972	9
GAGTACA	5315	0.0	19.858843	1
GTATAGT	300	3.6779966E-9	19.017975	1
TAAGGTG	915	0.0	18.674074	5
GAGTACT	3405	0.0	18.606104	12-13
AGTACAT	5490	0.0	18.590683	2
>>END_MODULE