FastQCFastQC Report
Mon 15 Aug 2016
SRR2920847_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920847_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2649987
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19750.043.5782851
GTATCAA35850.041.981141
TCAACGC43750.033.6564984
ATCAACG44650.033.1835633
CAACGCA44700.032.9412045
AACGCAG46550.031.8225236
TATCAAC47550.031.6421012
GTACATG73950.029.998841
TACATGG74500.028.9390052
ACATGGG78400.027.2653963
ACGCAGA56600.026.2476377
CGCAGAG59000.024.4018578
CATGGGG52300.023.593514
GCAGAGT67750.020.3024759
GAGTACA54850.019.719091
AGTACAT56950.017.8813882
TAGCCCT12850.017.8487914
CATGGGA32700.017.2543774
AGAGTAC59550.016.97759410-11
GTATAGC8200.016.8384861
Produced by FastQC (version 0.11.5)