##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2920847_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2649987 Sequences flagged as poor quality 0 Sequence length 98 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.799212222550526 31.0 30.0 33.0 23.0 34.0 2 28.83425239444571 31.0 30.0 34.0 22.0 34.0 3 28.914829770863026 31.0 30.0 34.0 22.0 34.0 4 32.4090378556574 35.0 33.0 37.0 25.0 37.0 5 32.372369373887494 35.0 33.0 37.0 25.0 37.0 6 32.495220919951684 35.0 33.0 37.0 25.0 37.0 7 32.33718957866586 35.0 33.0 37.0 23.0 37.0 8 32.27746588945531 35.0 33.0 37.0 23.0 37.0 9 33.57206016482345 37.0 33.0 39.0 23.0 39.0 10-11 33.382669990456556 37.0 33.0 39.0 22.5 39.0 12-13 33.21003404922364 37.0 33.0 39.0 20.5 39.0 14-15 34.004015114036406 38.0 33.0 40.0 19.0 41.0 16-17 33.89526533526391 38.0 33.0 40.0 18.5 41.0 18-19 33.77429549654394 38.0 33.0 40.0 18.0 41.0 20-21 33.67760294673144 38.0 32.0 40.0 18.0 41.0 22-23 33.45670941027258 38.0 32.0 40.0 17.5 41.0 24-25 33.26120618704922 38.0 32.0 40.0 16.5 41.0 26-27 33.32015741964017 38.0 32.0 40.0 16.0 41.0 28-29 33.17453821471577 38.0 32.0 40.0 15.5 41.0 30-31 32.93522779545711 38.0 32.0 40.0 10.0 41.0 32-33 32.70779592503661 38.0 31.0 40.0 9.5 41.0 34-35 32.38231225285256 37.5 30.5 40.0 8.5 41.0 36-37 32.078389063795406 37.0 30.0 40.0 8.0 41.0 38-39 31.88375924108307 37.0 30.0 40.0 7.0 41.0 40-41 31.69344000555474 37.0 30.0 40.0 2.0 41.0 42-43 31.462154153963773 37.0 30.0 40.0 2.0 41.0 44-45 31.191419618284918 36.5 30.0 40.0 2.0 41.0 46-47 30.943141985224834 36.0 29.0 39.0 2.0 40.5 48-49 30.648202991184483 36.0 28.5 39.0 2.0 40.0 50-51 29.510517221405237 34.5 27.0 38.0 2.0 39.5 52-53 29.98358369305208 35.0 28.0 38.5 2.0 39.5 54-55 30.644967692294337 36.0 29.0 39.0 2.0 40.0 56-57 30.594389708326872 36.0 29.0 39.0 2.0 41.0 58-59 30.357399677809738 35.5 28.0 39.0 2.0 41.0 60-61 30.032927897382137 35.0 28.0 39.0 2.0 40.0 62-63 29.64202994203368 35.0 28.0 39.0 2.0 40.0 64-65 29.216792572944698 34.5 27.0 38.0 2.0 40.0 66-67 28.83458390550595 34.0 26.0 38.0 2.0 40.0 68-69 28.3895083258899 34.0 26.0 37.0 2.0 40.0 70-71 28.04666702138539 34.0 26.0 37.0 2.0 39.0 72-73 27.580723603549753 33.5 25.5 36.0 2.0 39.0 74-75 27.123536832444838 33.0 24.5 36.0 2.0 38.5 76-77 26.70045003994359 33.0 23.5 35.0 2.0 37.5 78-79 26.230026600130493 33.0 22.0 35.0 2.0 37.0 80-81 25.780618357750434 32.0 20.5 35.0 2.0 36.5 82-83 25.307762641854467 32.0 19.0 35.0 2.0 36.0 84-85 24.904112737156822 32.0 17.5 35.0 2.0 35.5 86-87 24.448513521009726 31.5 10.5 34.0 2.0 35.0 88-89 23.914234862284232 31.0 4.5 34.0 2.0 35.0 90-91 23.425673975004408 31.0 2.0 34.0 2.0 35.0 92-93 22.88759775047953 31.0 2.0 34.0 2.0 35.0 94-95 22.24449572771489 30.0 2.0 34.0 2.0 35.0 96-97 21.59491008823817 30.0 2.0 34.0 2.0 35.0 98 21.197587761751283 29.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 127839.0 3 21561.0 4 15809.0 5 12873.0 6 12042.0 7 12092.0 8 13846.0 9 16057.0 10 17478.0 11 18799.0 12 19593.0 13 19619.0 14 18386.0 15 19480.0 16 20671.0 17 22813.0 18 23309.0 19 23519.0 20 24256.0 21 25872.0 22 28025.0 23 31699.0 24 36357.0 25 41037.0 26 47774.0 27 55510.0 28 64694.0 29 75234.0 30 88263.0 31 104265.0 32 124626.0 33 151811.0 34 191059.0 35 241734.0 36 310517.0 37 344307.0 38 205867.0 39 21293.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.27330697692388 17.21020764194472 11.918220389376938 25.598264991754473 2 16.585395129143045 19.302553193256156 38.512517603381056 25.599534074219743 3 20.23335670876005 22.711931960146533 28.032546444507012 29.022164886586406 4 13.5181037742899 14.958910127651482 35.4525924701403 36.07039362791832 5 14.895760400841693 36.22891759532729 33.74177415854383 15.133547845287184 6 34.77192030223159 33.87151382655201 16.91337414068837 14.443191730528026 7 30.70633662297627 30.483211045772823 20.014160473032263 18.79629185821864 8 27.33801600743914 33.98142780801942 19.67700568502601 19.003550499515427 9 27.172623865701983 15.650718286542537 18.637789543873236 38.538868303882246 10-11 26.326341662495263 24.873051240072357 27.062439752051564 21.738167345380816 12-13 26.88805693733689 21.984138410304997 27.077576347886996 24.050228304471123 14-15 23.426417398435007 23.69572777104486 24.487337619883494 28.390517210636634 16-17 24.272028037871657 26.884992685419846 25.106265669551554 23.736713607156947 18-19 24.12415153935172 25.828066071257012 26.490998356542505 23.556784032848764 20-21 24.500212619234826 25.48653172331855 26.323771887885083 23.689483769561537 22-23 24.23947347895168 25.267710963377976 26.272794454675303 24.220021102995045 24-25 24.17200750820431 25.376061576035674 26.37041531879094 24.081515596969076 26-27 23.869607044369065 25.755091675171283 26.20270026594816 24.1726010145115 28-29 23.913501904586962 25.91822198023806 26.310032435653053 23.85824367952193 30-31 23.92649652806094 25.64213505374785 26.172062594575983 24.259305823615225 32-33 23.864393998437844 25.68266948627188 26.141880667367793 24.311055847922482 34-35 24.34297690951876 25.72983688309643 25.897006674487233 24.03017953289758 36-37 24.445502544370036 25.276357496694864 26.084192138810575 24.193947820124528 38-39 24.009784594494857 25.533898566901104 26.244923047530456 24.211393791073586 40-41 24.267671626637732 25.435670316413777 26.284718944450823 24.011939112497664 42-43 24.27754929746646 25.266217402400216 26.057125361706113 24.399107938427214 44-45 24.066429417843818 25.500605370905223 26.147515024411828 24.28545018683913 46-47 24.099432105528134 25.57561300289308 25.91094766135139 24.414007230227398 48-49 24.116858067746815 25.521760177817775 25.961891753276316 24.399490001159087 50-51 23.977980661678615 25.61700699571939 26.090995487573498 24.3140168550285 52-53 24.098535371069143 25.460340104393282 26.136448051229415 24.304676473308156 54-55 23.686571811201453 25.274933101333314 26.390583608789143 24.64791147867609 56-57 23.680204266979278 25.589296519406158 26.352493908401915 24.37800530521265 58-59 23.743884857745694 25.175927246594398 26.357785567932794 24.72240232772711 60-61 23.716786061105047 25.180000992690836 26.653749056091204 24.449463890112913 62-63 23.54720958018503 25.33722481629165 26.5468322763016 24.568733327221718 64-65 23.3745981301504 25.183294342601094 26.48805784462264 24.95404968262586 66-67 23.5161883713635 25.28545610142811 26.33660326146189 24.8617522657465 68-69 23.563307772058415 24.93109897443601 26.58532976924005 24.920263484265522 70-71 23.377376114647692 24.976799573880932 26.508775640470933 25.137048671000446 72-73 23.58312830789882 24.924908152707424 26.471502016806564 25.02046152258719 74-75 23.431770849305153 25.066565809952486 26.414483236167886 25.08718010457448 76-77 23.494198035687447 24.83981232914104 26.543622344386552 25.122367290784965 78-79 23.527512182711906 24.90671783052326 26.487309233420458 25.078460753344377 80-81 23.404426915903137 25.097518918783834 26.4745595083123 25.02349465700073 82-83 23.504862632432243 24.95199082827937 26.31907849651025 25.224068042778143 84-85 23.37709173475836 24.752902975917106 26.47460272326383 25.39540256606071 86-87 23.45245594561894 24.721579491714582 26.603667299133676 25.222297263532802 88-89 23.39190832313761 24.547810761570812 26.550022922624166 25.510257992667412 90-91 23.377982098463562 24.70894431220019 26.595616382232656 25.317457207103587 92-93 23.602147362140002 24.388117646791567 26.64891333237922 25.360821658689215 94-95 23.404579982139587 24.206367462168735 26.738025014042783 25.651027541648897 96-97 23.572782750372866 24.138186942902223 26.65396581865188 25.635064488073027 98 23.469841710961877 24.140640115160604 26.545586993091348 25.84393118078617 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 28.0 1 27.0 2 66.0 3 111.0 4 107.0 5 126.5 6 215.5 7 379.5 8 589.5 9 717.5 10 725.5 11 760.0 12 888.5 13 1034.5 14 1173.0 15 1308.0 16 1409.0 17 1485.0 18 1511.5 19 1510.5 20 1564.5 21 1660.0 22 1833.0 23 2089.0 24 2447.5 25 3085.0 26 4016.0 27 5331.0 28 7048.0 29 9581.0 30 13277.5 31 18523.0 32 23703.5 33 28475.5 34 34059.0 35 40255.5 36 46936.0 37 52784.0 38 57815.0 39 63472.0 40 70535.5 41 78210.5 42 86257.0 43 92314.5 44 98163.5 45 104174.0 46 108335.5 47 115488.5 48 120927.5 49 121192.0 50 120953.0 51 121424.5 52 122961.0 53 122309.5 54 118091.0 55 112213.5 56 101809.0 57 90607.0 58 81302.5 59 69149.5 60 57225.0 61 48052.0 62 40522.5 63 31743.0 64 23270.0 65 18093.0 66 14528.0 67 11662.0 68 8481.5 69 6309.0 70 5290.0 71 4927.5 72 4159.0 73 2781.5 74 1993.5 75 1340.0 76 813.5 77 483.0 78 258.5 79 151.5 80 102.0 81 60.0 82 32.0 83 22.0 84 21.5 85 18.5 86 14.5 87 8.5 88 7.5 89 6.0 90 5.0 91 5.5 92 4.5 93 2.5 94 2.5 95 4.0 96 3.0 97 1.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3686433178728801 2 0.024075589804780174 3 0.01815103243902706 4 0.016188758661834945 5 0.021207651207345544 6 0.02369822946301246 7 0.013509500235284173 8 0.008264191484712944 9 0.0 10-11 1.886801708838572E-5 12-13 0.004773608323361586 14-15 0.002660390409462386 16-17 0.0053773848701899295 18-19 0.03767943012550627 20-21 0.07777396643832592 22-23 0.09417027328813311 24-25 0.06354748155368309 26-27 0.07224563743142891 28-29 0.05707575169236679 30-31 0.049924773215868606 32-33 0.016735931157398132 34-35 0.326812923987929 36-37 0.49754961062073133 38-39 0.4514173088396282 40-41 0.4630211393489855 42-43 0.41251145760337693 44-45 0.3880396394397407 46-47 0.3862660458334324 48-49 0.37664335711835567 50-51 0.37524712385381515 52-53 0.4340776011354018 54-55 0.4332662764006012 56-57 0.40362462155474726 58-59 0.3898321010631373 60-61 0.40362462155474726 62-63 0.3850962287739525 64-65 0.4354549663828539 66-67 0.4550777041547751 68-69 0.3967000592833097 70-71 0.35511494962050755 72-73 0.341680921453577 74-75 0.32513367046706265 76-77 0.3227940363481029 78-79 0.32222799583545125 80-81 0.3381526022580488 82-83 0.3330205016100079 84-85 0.3327563493707705 86-87 0.31996383378484494 88-89 0.36160554749891227 90-91 0.3411526169751022 92-93 0.33181294851635124 94-95 0.3377563738991927 96-97 0.3379639220871649 98 0.33230351696064925 >>END_MODULE >>Sequence Length Distribution pass #Length Count 98 2649987.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.99855226226917 #Duplication Level Percentage of deduplicated Percentage of total 1 75.62262547022578 40.835122936639955 2 12.307608166827752 13.291860456399588 3 4.861709350353362 7.875757993170561 4 2.3507101098328516 5.07739770877015 5 1.3056536572640647 3.525170362409824 6 0.8394418710643072 2.719718744748181 7 0.5497134085877439 2.0778609756026793 8 0.3966136342512594 1.7133249645636113 9 0.2818838074647396 1.3699185758344947 >10 1.3507532794176493 12.725756309006039 >50 0.08678680170605073 3.2134137488483665 >100 0.04396262810824171 4.352010788035296 >500 0.001762371455754032 0.6680618602546241 >1k 7.754434405227909E-4 0.5546245757166087 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 3.773603417677143E-5 0.0 7 0.0 0.0 0.0 3.773603417677143E-5 0.0 8 0.0 0.0 0.0 3.773603417677143E-5 0.0 9 0.0 0.0 0.0 1.132081025303143E-4 0.0 10-11 0.0 0.0 0.0 1.132081025303143E-4 0.0 12-13 0.0 0.0 0.0 1.5094413670708573E-4 0.0 14-15 0.0 0.0 0.0 1.5094413670708573E-4 0.0 16-17 0.0 0.0 0.0 1.8868017088385716E-4 0.0 18-19 0.0 0.0 0.0 3.773603417677143E-4 0.0 20-21 0.0 0.0 0.0 4.3396439303287145E-4 0.0 22-23 3.773603417677143E-5 0.0 0.0 6.226445639167287E-4 0.0 24-25 1.132081025303143E-4 0.0 0.0 9.434008544192858E-4 0.0 26-27 1.132081025303143E-4 0.0 0.0 0.0013396292132753858 0.0 28-29 1.8868017088385716E-4 0.0 0.0 0.005603801075250558 0.0 30-31 2.0754818797224286E-4 0.0 0.0 0.024660498334520133 0.0 32-33 2.264162050606286E-4 0.0 0.0 0.06386823784418565 0.0 34-35 2.264162050606286E-4 0.0 0.0 0.11511377225624125 0.0 36-37 2.6415223923740005E-4 0.0 0.0 0.19826512356475712 0.0 38-39 3.0188827341417145E-4 0.0 0.0 0.3379450540700766 0.0 40-41 3.396243075909429E-4 0.0 0.0 0.5024741630808001 0.0 42-43 3.9622835885610004E-4 0.0 0.0 0.6767769049433072 0.0 44-45 4.1509637594448577E-4 0.0 0.0 0.8841552807617548 0.0 46-47 4.528324101212572E-4 0.0 0.0 1.1382131308568684 0.0 48-49 4.905684442980286E-4 0.0 0.0 1.414289956894128 0.0 50-51 4.905684442980286E-4 0.0 0.0 1.7303481111416774 0.0 52-53 4.905684442980286E-4 0.0 0.0 2.049425148123368 0.0 54-55 4.905684442980286E-4 0.0 0.0 2.396766474703461 0.0 56-57 4.905684442980286E-4 0.0 0.0 2.8059382932821935 0.0 58-59 4.905684442980286E-4 0.0 0.0 3.25118576053392 0.0 60-61 5.094364613864144E-4 0.0 0.0 3.736791916337703 0.0 62-63 5.283044784748001E-4 0.0 0.0 4.2261905435762515 0.0 64-65 5.283044784748001E-4 0.0 0.0 4.733740203253827 0.0 66-67 5.283044784748001E-4 0.0 0.0 5.312610967525501 0.0 68-69 5.283044784748001E-4 0.0 0.0 5.957312243418553 0.0 70-71 5.660405126515716E-4 0.0 0.0 6.659862859704594 0.0 72-73 6.037765468283429E-4 0.0 0.0 7.372394657030393 0.0 74-75 6.415125810051144E-4 0.0 0.0 8.08692646416756 0.0 76-77 6.415125810051144E-4 0.0 0.0 8.862722722790714 0.0 78-79 6.415125810051144E-4 0.0 0.0 9.70693441137636 0.0 80-81 7.924567177122001E-4 0.0 0.0 10.604278436082893 0.0 82-83 7.924567177122001E-4 0.0 3.773603417677143E-5 11.529698070216948 0.0 84-85 7.924567177122001E-4 0.0 3.773603417677143E-5 12.44053272714168 0.0 86 7.924567177122001E-4 0.0 3.773603417677143E-5 13.13772482657462 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1975 0.0 43.578285 1 GTATCAA 3585 0.0 41.98114 1 TCAACGC 4375 0.0 33.656498 4 ATCAACG 4465 0.0 33.183563 3 CAACGCA 4470 0.0 32.941204 5 AACGCAG 4655 0.0 31.822523 6 TATCAAC 4755 0.0 31.642101 2 GTACATG 7395 0.0 29.99884 1 TACATGG 7450 0.0 28.939005 2 ACATGGG 7840 0.0 27.265396 3 ACGCAGA 5660 0.0 26.247637 7 CGCAGAG 5900 0.0 24.401857 8 CATGGGG 5230 0.0 23.59351 4 GCAGAGT 6775 0.0 20.302475 9 GAGTACA 5485 0.0 19.71909 1 AGTACAT 5695 0.0 17.881388 2 TAGCCCT 1285 0.0 17.848791 4 CATGGGA 3270 0.0 17.254377 4 AGAGTAC 5955 0.0 16.977594 10-11 GTATAGC 820 0.0 16.838486 1 >>END_MODULE