FastQCFastQC Report
Mon 15 Aug 2016
SRR2920847_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920847_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2649987
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20350.045.572251
GTATCAA36300.040.7459141
TCAACGC43600.032.9986654
ATCAACG45700.031.794023
CAACGCA45800.031.4100135
AACGCAG47950.030.2986816
TATCAAC48350.029.8527582
GTACATG74300.028.2279991
TACATGG78050.026.4620632
ACATGGG77850.025.5561623
ACGCAGA58600.024.7106957
CGCAGAG61150.023.2095768
CATGGGG53900.021.3189984
GAGTACA56300.021.2029191
GGACCGT4200.019.217226
AGTACAT55800.018.7195682
GCAGAGT70250.018.7154929
TAAGACG1350.00942268517.586384
TAAGGTG10050.017.4794045
ATGGGGA35850.016.6867715
Produced by FastQC (version 0.11.5)