##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920847_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2649987
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.845038107734112	31.0	30.0	34.0	26.0	34.0
2	29.85567665048923	31.0	30.0	34.0	26.0	34.0
3	29.998430180978247	31.0	30.0	34.0	26.0	34.0
4	33.62135587834959	35.0	35.0	37.0	28.0	37.0
5	33.09495895640243	35.0	33.0	37.0	26.0	37.0
6	33.03255978236874	35.0	33.0	37.0	26.0	37.0
7	32.832447479931034	35.0	33.0	37.0	26.0	37.0
8	32.750006698146066	35.0	33.0	37.0	26.0	37.0
9	34.0388530207884	37.0	33.0	39.0	26.0	39.0
10-11	33.819172131787816	37.0	33.0	39.0	25.0	39.0
12-13	33.61117563972955	37.0	33.0	39.0	23.5	39.0
14-15	34.453235619646435	38.0	33.0	40.0	24.0	41.0
16-17	34.30756245219317	38.0	33.0	40.0	22.5	41.0
18-19	34.194893974951576	38.0	33.0	40.0	22.0	41.0
20-21	34.06405955953746	38.0	33.0	40.0	19.5	41.0
22-23	33.907833698806826	38.0	32.5	40.0	19.0	41.0
24-25	33.70386684915813	38.0	32.0	40.0	18.0	41.0
26-27	33.5074270175665	38.0	32.0	40.0	18.0	41.0
28-29	33.44152386407933	38.0	32.0	40.0	17.5	41.0
30-31	33.42718417109216	38.0	32.0	40.0	17.0	41.0
32-33	33.27810211899153	38.0	32.0	40.0	16.0	41.0
34-35	33.08918704129492	38.0	32.0	40.0	16.0	41.0
36-37	32.87668996112056	38.0	31.0	40.0	15.0	41.0
38-39	32.67822389317382	37.0	31.0	40.0	11.0	41.0
40-41	32.43733101332195	37.0	31.0	40.0	9.0	41.0
42-43	32.45176372563337	37.0	31.0	40.0	9.0	41.0
44-45	32.29095444619162	37.0	31.0	40.0	8.5	41.0
46-47	32.02325860466485	37.0	30.0	40.0	7.5	41.0
48-49	31.734267375651278	37.0	30.0	40.0	4.0	41.0
50-51	31.447524836914294	36.0	30.0	40.0	2.0	41.0
52-53	31.16503760207126	36.0	29.5	39.0	2.0	41.0
54-55	30.864152541125673	36.0	29.0	39.0	2.0	40.5
56-57	30.52413559009912	35.0	28.0	39.0	2.0	40.0
58-59	30.170098947655212	35.0	28.0	39.0	2.0	40.0
60-61	29.814889469269094	35.0	28.0	38.5	2.0	40.0
62-63	29.47562308796232	34.0	27.5	38.0	2.0	40.0
64-65	29.051639498608864	34.0	26.0	38.0	2.0	40.0
66-67	28.60325767635841	34.0	26.0	37.0	2.0	40.0
68-69	28.143791271428878	34.0	26.0	37.0	2.0	39.0
70-71	27.69595171598955	33.0	25.0	36.0	2.0	39.0
72-73	27.233400012905726	33.0	24.5	36.0	2.0	39.0
74-75	26.76490016743478	32.5	23.5	35.0	2.0	38.0
76-77	25.417756011633266	30.5	21.5	34.0	2.0	36.0
78-79	26.23272434921379	32.0	23.0	35.0	2.0	36.5
80-81	26.24501629630636	32.0	23.0	35.0	2.0	36.0
82-83	25.884122450412022	32.0	21.5	35.0	2.0	36.0
84-85	25.54468021918598	32.0	20.5	35.0	2.0	35.5
86-87	24.969327208020268	32.0	18.0	34.5	2.0	35.0
88-89	24.576349619828324	31.0	16.5	34.0	2.0	35.0
90-91	24.174429723617514	31.0	9.0	34.0	2.0	35.0
92-93	23.82189931497777	31.0	4.5	34.0	2.0	35.0
94-95	23.389499080561528	31.0	2.0	34.0	2.0	35.0
96-97	22.795992584114565	31.0	2.0	34.0	2.0	35.0
98-99	22.11790944634823	30.5	2.0	34.0	2.0	35.0
100-101	19.920575459426782	27.0	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	80558.0
3	34505.0
4	18581.0
5	12931.0
6	11092.0
7	11064.0
8	12136.0
9	13996.0
10	15906.0
11	17915.0
12	19288.0
13	21322.0
14	22741.0
15	24136.0
16	25133.0
17	26453.0
18	25459.0
19	25115.0
20	24930.0
21	26164.0
22	28126.0
23	31165.0
24	35768.0
25	42113.0
26	50011.0
27	59161.0
28	69475.0
29	80786.0
30	94201.0
31	110599.0
32	131433.0
33	159605.0
34	197232.0
35	251542.0
36	320740.0
37	342070.0
38	167496.0
39	9039.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.10077912081722	18.842028897295855	12.745386764737571	25.311805217149352
2	16.802157897378365	20.74753574262817	37.107276375318065	25.343029984675397
3	20.10026146632489	23.78501942881987	28.50496212361379	27.60975698124145
4	14.254211246773608	16.184932920848226	34.93218489668939	34.62867093568878
5	14.896898657267284	36.55244507174073	33.82191997947031	14.728736291521688
6	34.70657452125118	34.554455258540415	16.598111635971467	14.14085858423694
7	30.516860895720914	30.74895327922127	20.081810486857417	18.652375338200397
8	27.22384890604988	34.546797489827455	19.5009124122063	18.728441191916367
9	26.934542647247206	15.631158248590795	18.575403319819117	38.858895784342884
10-11	26.35522492088032	25.168264485913344	27.028150644243365	21.448359948962967
12-13	26.888166506428725	22.09627120949112	27.123480179290205	23.89208210478995
14-15	23.432276592826927	23.830457352536175	24.46758434613166	28.26968170850524
16-17	24.257985220875042	27.068114623177625	25.05516684099036	23.618733314956973
18-19	24.144841328661332	25.995143078282318	26.479249131458456	23.380766461597897
20-21	24.54979827769258	25.51336557744485	26.281712870119144	23.655123274743424
22-23	24.240809450767873	25.387410520042625	26.316969170874927	24.054810858314582
24-25	24.208535733764215	25.608069700695612	26.29858625057084	23.884808314969337
26-27	23.902506899628484	25.925856699936677	26.174088510839468	23.99754788959537
28-29	24.028244807204274	25.988676784387966	26.28131744924702	23.701760959160733
30-31	24.02752499269639	25.761972450850656	26.138589719684724	24.071912836768234
32-33	23.929322134121403	25.788939158217023	26.086635667072894	24.19510304058868
34-35	24.379859444117336	25.763621540830655	25.95692362274861	23.899595392303397
36-37	24.4507050972106	25.421037076627446	26.087388949218127	24.040868876943826
38-39	24.066397638044204	25.558002242597233	26.333304456260763	24.0422956630978
40-41	24.18346812053526	25.486038856686456	26.33290422619262	23.997588796585664
42-43	24.32042121694288	25.36161297919428	26.109219460941347	24.208746342921497
44-45	24.049686880853173	25.589049379253698	26.13217172313858	24.229092016754546
46-47	24.058929533996384	25.641806245290965	25.97249547404389	24.326768746668762
48-49	24.131346185561643	25.516905109555164	26.05421772001324	24.297530984869955
50-51	23.971659712747467	25.597782294208336	26.13582881847535	24.294729174568847
52-53	24.205547412420202	25.46910875186617	26.070189130398685	24.255154705314943
54-55	23.799289877049397	25.359345008193436	26.237072977059135	24.60429213769803
56-57	23.794483422549092	25.61975689562747	26.160372504174294	24.425387177649146
58-59	23.76188135578829	25.30656374444672	26.242316919805386	24.689237979959604
60-61	23.756974162666424	25.405824364710494	26.36463726805318	24.472564204569903
62-63	23.55359292335312	25.528039748963057	26.271342036989058	24.647025290694767
64-65	23.312136885027822	25.396376728186688	26.19814968214762	25.09333670463787
66-67	23.44673446309768	25.578722513500114	26.005399478549972	24.969143544852233
68-69	23.443589025015733	25.289667159422503	26.254261255265902	25.01248256029586
70-71	23.289674495917918	25.25484115632541	26.136648644649853	25.318835703106824
72-73	23.46719907672636	25.292944767615804	26.05505324887106	25.184802906786775
74-75	23.326638426699457	25.42626211201518	25.862173802696898	25.384925658588465
76-77	23.299279167811694	25.273080464904897	26.102454513697854	25.32518585358556
78-79	23.317078918507374	25.324540691930498	26.005081015266462	25.353299374295666
80-81	23.112104482095923	25.601759383808055	25.927850770919292	25.358285363176737
82-83	23.216004921339707	25.510824871635684	25.788027633473416	25.485142573551194
84-85	23.049802040676532	25.298429742225466	25.82583693269383	25.825931284404174
86-87	23.109070510498423	25.295372461841676	25.912212347731305	25.683344679928595
88-89	22.93410116805978	25.254330698347	25.862005792932564	25.94956234066066
90-91	22.812340142808278	25.476885217617664	25.82306589824229	25.887708741331767
92-93	22.921404946972913	25.250082742397357	25.8360579628481	25.992454347781635
94-95	22.64861778628443	25.124926791126782	25.826826297231044	26.399629125357745
96-97	22.74045607220442	25.021552980707888	25.81931045179463	26.418680495293064
98-99	22.573251710250634	25.165458824168724	25.74093250170572	26.52035696387492
100-101	22.64564814620884	24.93632195138335	25.882770500331432	26.535259402076377
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	39.0
1	36.0
2	44.5
3	75.5
4	78.5
5	81.0
6	160.0
7	285.5
8	426.5
9	486.5
10	405.0
11	353.5
12	358.0
13	378.0
14	456.0
15	510.5
16	544.0
17	597.5
18	668.0
19	810.5
20	957.0
21	1098.0
22	1339.5
23	1617.0
24	1988.0
25	2546.5
26	3286.0
27	4427.0
28	5857.5
29	7840.5
30	10808.0
31	15023.0
32	20632.0
33	25410.5
34	29707.0
35	34980.5
36	41343.0
37	48167.5
38	53372.0
39	58029.5
40	63836.0
41	71272.0
42	79637.0
43	87233.0
44	92792.0
45	98895.5
46	104386.0
47	108769.0
48	115596.5
49	120269.5
50	120598.0
51	120374.0
52	120733.5
53	120139.0
54	117522.5
55	110849.0
56	99883.0
57	88937.0
58	79400.0
59	68574.5
60	57327.0
61	47689.5
62	40004.0
63	32140.5
64	24492.0
65	18862.5
66	14542.5
67	11839.0
68	9050.5
69	6361.5
70	4967.5
71	4579.0
72	4317.0
73	3233.5
74	2118.0
75	1455.5
76	910.5
77	508.5
78	291.5
79	179.0
80	97.0
81	52.5
82	25.0
83	13.0
84	7.5
85	7.5
86	5.5
87	2.0
88	2.5
89	2.5
90	0.5
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18211410093709893
2	0.0
3	0.011886850765683
4	0.01343402816693063
5	0.00630191770752083
6	0.10271748502917184
7	0.13686859595915
8	0.11913265989606742
9	0.13728369233509446
10-11	0.10903827075378107
12-13	0.10875525049745527
14-15	0.1112458287531222
16-17	0.06252860863091027
18-19	0.07598150481492928
20-21	0.03488696359642519
22-23	0.0799060523693135
24-25	0.05518894998352822
26-27	0.06249087259673349
28-29	0.03790584633056691
30-31	0.02313218895036089
32-33	0.03066052776862679
34-35	0.019132169327623117
36-37	0.02400011773642663
38-39	0.03118883224710159
40-41	0.025943523496530358
42-43	0.012490627312511344
44-45	0.025509559103497486
46-47	0.03105675612748289
48-49	0.008528343723950343
50-51	0.015169885739062116
52-53	0.0066038059809350015
54-55	0.011509490423915287
56-57	0.005188704699306072
58-59	0.0033773750588210433
60-61	0.021320859309875862
62-63	0.009717028800518644
64-65	0.006490597878404687
66-67	0.03877377511663265
68-69	0.0865287263673369
70-71	0.020471798540898502
72-73	0.04345304335455231
74-75	0.08313248329142747
76-77	0.23798229953580904
78-79	0.07890604746362906
80-81	0.015962342456774317
82-83	0.011396282321384973
84-85	0.012320815158715874
86-87	0.2549635149153562
88-89	0.6136067837313919
90-91	0.26426544733993035
92-93	0.3494734125110803
94-95	0.0013207611961870002
96-97	0.0045849281524777295
98-99	0.0031320908366720287
100-101	0.005037760562598986
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2649987.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.45598332680406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.31908443564393	40.26255721781929
2	12.435065045417375	13.294572594711102
3	4.945338776814904	7.930738415964453
4	2.3700126618685107	5.067654293486305
5	1.3410782051732815	3.5844327087841625
6	0.8345414914735196	2.6766741632240794
7	0.5634696313965032	2.1084576254764333
8	0.4001656880718919	1.7113000279624102
9	0.28847708083291695	1.3878743420852564
>10	1.3645460364564548	12.885733891042403
>50	0.08808089198121334	3.2485574241341846
>100	0.047014374477139874	4.461162404243848
>500	0.0023442602942629867	0.8428365565490173
>1k	7.814200980678673E-4	0.5374483345171541
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.773603417677143E-5	0.0
5	3.773603417677143E-5	0.0	0.0	3.773603417677143E-5	0.0
6	3.773603417677143E-5	0.0	0.0	3.773603417677143E-5	0.0
7	1.132081025303143E-4	0.0	0.0	3.773603417677143E-5	0.0
8	1.132081025303143E-4	0.0	0.0	3.773603417677143E-5	0.0
9	1.132081025303143E-4	0.0	0.0	3.773603417677143E-5	0.0
10-11	1.132081025303143E-4	0.0	0.0	3.773603417677143E-5	0.0
12-13	1.132081025303143E-4	0.0	0.0	3.773603417677143E-5	0.0
14-15	1.132081025303143E-4	3.773603417677143E-5	0.0	3.773603417677143E-5	0.0
16-17	1.320761196187E-4	3.773603417677143E-5	0.0	3.773603417677143E-5	0.0
18-19	1.8868017088385716E-4	3.773603417677143E-5	0.0	2.6415223923740005E-4	0.0
20-21	2.264162050606286E-4	3.773603417677143E-5	0.0	3.207562905025572E-4	0.0
22-23	2.264162050606286E-4	3.773603417677143E-5	0.0	4.717004272096429E-4	0.0
24-25	2.6415223923740005E-4	3.773603417677143E-5	0.0	7.547206835354286E-4	0.0
26-27	2.6415223923740005E-4	3.773603417677143E-5	0.0	0.0011320810253031429	0.0
28-29	3.207562905025572E-4	3.773603417677143E-5	0.0	0.005358516853101543	0.0
30-31	3.396243075909429E-4	3.773603417677143E-5	0.0	0.023622757394658918	0.0
32-33	3.396243075909429E-4	3.773603417677143E-5	0.0	0.061415395622695504	0.0
34-35	3.396243075909429E-4	3.773603417677143E-5	0.0	0.11084960039426608	0.0
36-37	3.396243075909429E-4	3.773603417677143E-5	0.0	0.1916424495667337	0.0
38-39	3.773603417677143E-4	3.773603417677143E-5	0.0	0.32741670053475735	0.0
40-41	3.773603417677143E-4	3.773603417677143E-5	0.0	0.4876439016493288	0.0
42-43	3.9622835885610004E-4	3.773603417677143E-5	0.0	0.6581353040599822	0.0
44-45	4.1509637594448577E-4	3.773603417677143E-5	0.0	0.8614947922386034	0.0
46-47	4.717004272096429E-4	3.773603417677143E-5	0.0	1.107080902661032	0.0
48-49	4.905684442980286E-4	3.773603417677143E-5	0.0	1.3740633444616899	0.0
50-51	4.905684442980286E-4	7.547206835354286E-5	0.0	1.6770270948498993	0.0
52-53	5.471724955631858E-4	7.547206835354286E-5	0.0	1.9827078396988362	0.0
54-55	5.660405126515716E-4	7.547206835354286E-5	0.0	2.314784940454425	0.0
56-57	5.660405126515716E-4	7.547206835354286E-5	0.0	2.7082585688156207	0.0
58-59	5.660405126515716E-4	7.547206835354286E-5	0.0	3.1371852012858934	0.0
60-61	5.660405126515716E-4	7.547206835354286E-5	0.0	3.6052818372316544	0.0
62-63	5.660405126515716E-4	7.547206835354286E-5	0.0	4.07566150324511	0.0
64-65	6.037765468283429E-4	7.547206835354286E-5	0.0	4.564720506176068	1.8868017088385716E-5
66-67	6.037765468283429E-4	7.547206835354286E-5	0.0	5.118421335651835	3.773603417677143E-5
68-69	6.037765468283429E-4	7.547206835354286E-5	0.0	5.735933798920523	3.773603417677143E-5
70-71	6.037765468283429E-4	7.547206835354286E-5	0.0	6.408484268036032	3.773603417677143E-5
72-73	6.037765468283429E-4	7.547206835354286E-5	0.0	7.0955819783266865	3.773603417677143E-5
74-75	6.226445639167286E-4	7.547206835354286E-5	0.0	7.787321220821083	3.773603417677143E-5
76-77	6.415125810051144E-4	7.547206835354286E-5	0.0	8.53857018921225	3.773603417677143E-5
78-79	6.415125810051144E-4	7.547206835354286E-5	0.0	9.354253435960253	3.773603417677143E-5
80-81	7.924567177122001E-4	7.547206835354286E-5	0.0	10.223144490897504	3.773603417677143E-5
82-83	7.924567177122001E-4	7.547206835354286E-5	0.0	11.116526232015477	3.773603417677143E-5
84-85	8.113247348005858E-4	7.547206835354286E-5	0.0	11.997323005735499	3.773603417677143E-5
86-87	8.490607689773573E-4	7.547206835354286E-5	0.0	12.901704800816004	3.773603417677143E-5
88-89	8.67928786065743E-4	7.547206835354286E-5	0.0	13.857237790223122	3.773603417677143E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2035	0.0	45.57225	1
GTATCAA	3630	0.0	40.745914	1
TCAACGC	4360	0.0	32.998665	4
ATCAACG	4570	0.0	31.79402	3
CAACGCA	4580	0.0	31.410013	5
AACGCAG	4795	0.0	30.298681	6
TATCAAC	4835	0.0	29.852758	2
GTACATG	7430	0.0	28.227999	1
TACATGG	7805	0.0	26.462063	2
ACATGGG	7785	0.0	25.556162	3
ACGCAGA	5860	0.0	24.710695	7
CGCAGAG	6115	0.0	23.209576	8
CATGGGG	5390	0.0	21.318998	4
GAGTACA	5630	0.0	21.202919	1
GGACCGT	420	0.0	19.21722	6
AGTACAT	5580	0.0	18.719568	2
GCAGAGT	7025	0.0	18.715492	9
TAAGACG	135	0.009422685	17.58638	4
TAAGGTG	1005	0.0	17.479404	5
ATGGGGA	3585	0.0	16.686771	5
>>END_MODULE