FastQCFastQC Report
Mon 15 Aug 2016
SRR2920846_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920846_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2428974
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33700.040.018081
GGTATCA20200.034.1789321
TCAACGC38800.033.806694
ATCAACG39150.033.3873143
CAACGCA39350.033.3341675
TATCAAC42250.031.266472
AACGCAG43700.030.4326786
GTACATG68750.025.0390261
ACGCAGA53400.024.5565817
TACATGG68000.024.5530552
CGCAGAG54400.023.6818038
ACATGGG70950.022.4308343
GAGTACA51100.021.797821
AGTACAT50850.020.5663362
GCAGAGT62700.019.9619269
TATACCG1406.147336E-419.6558385
GTATAGG6600.019.526871
GTATTAG6450.019.267381
CATGGGG51900.018.3807964
GTGTAGC14800.017.4158571
Produced by FastQC (version 0.11.5)