##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920846_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2428974
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.611214446922858	31.0	30.0	33.0	22.0	34.0
2	28.644661079122297	31.0	28.0	34.0	18.0	34.0
3	28.72985836818344	31.0	28.0	34.0	22.0	34.0
4	32.1957336719125	35.0	32.0	37.0	22.0	37.0
5	32.160752235305935	35.0	32.0	37.0	22.0	37.0
6	32.27508116595731	35.0	32.0	37.0	23.0	37.0
7	32.10822841249021	35.0	32.0	37.0	22.0	37.0
8	32.052895996416595	35.0	32.0	37.0	22.0	37.0
9	33.32260205337727	37.0	33.0	39.0	22.0	39.0
10-11	33.1317795085497	37.0	32.5	39.0	19.0	39.0
12-13	32.94805934522148	37.0	32.0	39.0	18.0	39.0
14-15	33.72032306644699	38.0	32.5	40.0	17.0	41.0
16-17	33.60736199728774	38.0	32.0	40.0	17.0	41.0
18-19	33.49110529795708	38.0	32.0	40.0	17.5	41.0
20-21	33.40081017746588	38.0	32.0	40.0	17.5	41.0
22-23	33.18098855730856	38.0	32.0	40.0	15.5	41.0
24-25	32.99751849958048	38.0	32.0	40.0	12.0	41.0
26-27	33.03836702245475	38.0	32.0	40.0	10.5	41.0
28-29	32.87885564028269	38.0	32.0	40.0	10.0	41.0
30-31	32.64240807023871	38.0	31.0	40.0	9.0	41.0
32-33	32.406113651278275	37.5	31.0	40.0	8.5	41.0
34-35	32.09083732473053	37.0	30.0	40.0	7.5	41.0
36-37	31.792581970823896	37.0	30.0	40.0	4.0	41.0
38-39	31.60330369942206	37.0	30.0	40.0	2.0	41.0
40-41	31.40314511394523	37.0	30.0	40.0	2.0	41.0
42-43	31.181242985721543	37.0	30.0	40.0	2.0	41.0
44-45	30.907110574258926	36.0	29.0	39.5	2.0	41.0
46-47	30.665821247983715	36.0	28.5	39.0	2.0	40.5
48-49	30.37524815004195	36.0	28.0	39.0	2.0	40.0
50-51	29.245880359361607	34.0	26.5	38.0	2.0	39.5
52-53	29.70551496228449	35.0	27.5	38.5	2.0	39.5
54-55	30.366721916331752	36.0	28.0	39.0	2.0	40.0
56-57	30.32589047886062	36.0	28.0	39.0	2.0	41.0
58-59	30.08665490038181	35.0	28.0	39.0	2.0	41.0
60-61	29.759745884476324	35.0	28.0	39.0	2.0	40.0
62-63	29.370417303766942	35.0	27.0	39.0	2.0	40.0
64-65	28.9561271549222	34.0	26.0	38.0	2.0	40.0
66-67	28.571396811987285	34.0	26.0	38.0	2.0	40.0
68-69	28.116990548272646	34.0	26.0	37.0	2.0	40.0
70-71	27.78124426198057	34.0	25.5	37.0	2.0	39.0
72-73	27.313351233895464	33.5	24.5	36.0	2.0	39.0
74-75	26.866725415751674	33.0	23.5	36.0	2.0	39.0
76-77	26.442409840533493	33.0	22.0	35.0	2.0	37.5
78-79	25.980711403250922	32.0	20.5	35.0	2.0	37.0
80-81	25.53429225673062	32.0	19.5	35.0	2.0	36.5
82-83	25.062347106226746	32.0	17.5	35.0	2.0	36.0
84-85	24.66244471945768	32.0	13.0	35.0	2.0	35.5
86-87	24.207914329260007	31.0	7.0	34.0	2.0	35.0
88-89	23.687323124907884	31.0	2.0	34.0	2.0	35.0
90-91	23.194715752412336	31.0	2.0	34.0	2.0	35.0
92-93	22.65627832986273	30.5	2.0	34.0	2.0	35.0
94-95	22.032740778616816	30.0	2.0	34.0	2.0	35.0
96-97	21.393597461314943	29.5	2.0	34.0	2.0	35.0
98	21.011561671718184	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	125119.0
3	21533.0
4	15788.0
5	12666.0
6	11692.0
7	11616.0
8	13445.0
9	15416.0
10	16919.0
11	18199.0
12	18548.0
13	18653.0
14	17484.0
15	18451.0
16	19951.0
17	21280.0
18	21955.0
19	21986.0
20	22611.0
21	24264.0
22	26244.0
23	29102.0
24	33573.0
25	38755.0
26	44108.0
27	50993.0
28	59529.0
29	68832.0
30	80362.0
31	94758.0
32	112637.0
33	137677.0
34	170958.0
35	215840.0
36	276005.0
37	309300.0
38	191731.0
39	20994.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.05259808287169	17.47281244253058	12.016910733067053	25.457678741530685
2	16.386939437091847	19.03634532215303	38.93621016687271	25.640505073882412
3	20.495330412439056	22.382395572539203	28.02411384899196	29.09816016602978
4	13.403504755347784	14.535753948171918	35.82617296313941	36.23456833334088
5	14.630819862230728	36.47068367424255	34.08928812621273	14.809208337313992
6	35.03568569574341	34.08980474579398	16.597842880814884	14.276666677647725
7	30.5109667579671	30.57544803369167	19.933814046871216	18.97977116147001
8	27.276436639394646	34.61114215142743	19.23041608800609	18.882005121171833
9	26.902017065641708	15.611653315350432	18.85396879505503	38.63236082395283
10-11	26.23100380284178	25.12321050913287	27.024775491534896	21.62101019649045
12-13	26.673197872904563	22.059536768810943	27.10152779996476	24.165737558319734
14-15	23.450832015002725	23.806920232163424	24.485212910822526	28.25703484201133
16-17	23.93038278393519	26.977062360825165	25.323052365454977	23.769502489784667
18-19	23.847676572902042	26.312151126598405	26.579996116278537	23.260176184221013
20-21	24.401098207224294	25.549036658341734	26.599552171744545	23.450312962689427
22-23	24.061758982461605	25.466380005950807	26.407876658416313	24.06398435317128
24-25	23.8329599181336	25.584341403317463	26.637827491858985	23.94487118668995
26-27	23.752895428375073	25.892133911141762	26.452514150620782	23.90245650986238
28-29	23.72602947388888	26.06071953132772	26.38769683776385	23.82555415701955
30-31	24.020697096635537	25.782706024076035	26.327216871556775	23.86938000773165
32-33	23.71130242427462	25.834689120227104	26.326169590101458	24.127838865396818
34-35	24.015277221845636	25.8643316277404	26.290502668680826	23.829888481733136
36-37	24.069570708612083	25.680016039690372	26.312996183952404	23.93741706774514
38-39	23.844128458183707	25.87849711726249	26.354220676415945	23.92315374813786
40-41	24.061084863805647	25.677951973842873	26.375770407021452	23.885192755330024
42-43	23.895840831804698	25.601781399049884	26.311649321896997	24.19072844724842
44-45	23.896733448883225	25.736857921995142	26.4016142813385	23.964794347783133
46-47	23.940859861698254	25.644272101183734	26.261210820257748	24.153657216860264
48-49	23.82250985452461	25.80308640776177	26.25684712250361	24.117556615210002
50-51	23.783867672668276	25.87740135492389	26.303369785771753	24.035361186636084
52-53	23.90546908763071	25.709136926732135	26.331064971909125	24.054329013728033
54-55	23.544877812746844	25.554857093229554	26.654128808400294	24.2461362856233
56-57	23.584976646941527	25.739527299533105	26.516811845501444	24.158684208023924
58-59	23.575013284891106	25.61165788206902	26.552131028813797	24.26119780422607
60-61	23.629169092159923	25.52460938533591	26.7106962598894	24.13552526261477
62-63	23.545105917293675	25.60384586370208	26.68382093833866	24.167227280665585
64-65	23.400981619042813	25.439611157107844	26.664422430342515	24.494984793506823
66-67	23.37037450673814	25.546951910588273	26.55031064121973	24.53236294145385
68-69	23.48636884998366	25.3783031439773	26.607112700613268	24.528215305425775
70-71	23.365193480092074	25.234508958354812	26.630333385881467	24.769964175671646
72-73	23.446473167739768	25.233217790927537	26.588945651273477	24.731363390059222
74-75	23.344982495275275	25.353388894052525	26.569776208032554	24.73185240263965
76-77	23.337429749898277	25.120672170322806	26.66802295921002	24.8738751205689
78-79	23.27914359420278	25.12928497220977	26.6983639807255	24.893207452861947
80-81	23.351872123046387	25.15959520256914	26.61930024142499	24.86923243295948
82-83	23.395733855088892	25.160852138319207	26.628021115896992	24.81539289069491
84-85	23.34488792473245	25.072705928866657	26.62401362341479	24.958392522986102
86-87	23.265845304955988	25.029818474421788	26.7484365825333	24.955899638088916
88-89	23.30328167274353	24.91950890825937	26.684362102674026	25.09284731632307
90-91	23.38637140199898	24.82451823857587	26.696860809052623	25.092249550372532
92-93	23.46479785604837	24.698780497377278	26.778966337365016	25.05745530920933
94-95	23.343877579055505	24.5593613215473	26.864752231571547	25.232008867825645
96-97	23.41273185790399	24.433555275717552	26.8472613334391	25.306451532939356
98	23.491365242674647	24.332284267398617	26.694211188622823	25.482139301303906
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	25.0
1	25.5
2	49.0
3	78.0
4	80.0
5	103.5
6	177.5
7	281.5
8	456.0
9	572.0
10	599.0
11	691.5
12	779.5
13	906.5
14	1044.0
15	1150.0
16	1259.0
17	1313.5
18	1371.5
19	1413.0
20	1414.5
21	1486.0
22	1693.5
23	2002.5
24	2449.5
25	3120.5
26	4230.5
27	5757.5
28	7839.0
29	10625.0
30	14364.0
31	19465.5
32	24230.5
33	28803.5
34	34156.5
35	40021.0
36	46303.0
37	52109.5
38	57494.5
39	63486.0
40	69566.0
41	74443.5
42	78941.5
43	84225.0
44	89759.0
45	94650.0
46	99184.0
47	103964.0
48	107271.0
49	107909.5
50	107758.0
51	108409.0
52	108390.5
53	107120.0
54	104391.5
55	98761.5
56	91381.0
57	83329.5
58	74842.5
59	64563.0
60	53771.0
61	44468.0
62	36133.5
63	27931.0
64	20632.0
65	15385.0
66	11648.0
67	8985.5
68	6705.5
69	4916.5
70	3624.5
71	2716.0
72	2097.0
73	1627.0
74	1265.5
75	906.5
76	601.5
77	389.5
78	243.0
79	154.5
80	88.5
81	54.5
82	31.0
83	18.0
84	15.5
85	13.5
86	11.0
87	6.5
88	5.0
89	2.5
90	0.5
91	0.0
92	1.0
93	2.5
94	2.5
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3791312710634202
2	0.027007287850755093
3	0.02033780518029423
4	0.01832049252071039
5	0.02330201970049906
6	0.022643305362675763
7	0.014903411893252047
8	0.009592527544551732
9	0.0
10-11	4.1169646113955936E-5
12-13	0.005022696825902624
14-15	0.0033553261582874084
16-17	0.006154862094036412
18-19	0.03666156986447776
20-21	0.07935449288465006
22-23	0.09930118642686173
24-25	0.06605669718984229
26-27	0.0770695775253255
28-29	0.059099026996583745
30-31	0.05312942831006013
32-33	0.020378974826408186
34-35	0.3340916782147524
36-37	0.5133237325718596
38-39	0.4690663629993569
40-41	0.47686801093795156
42-43	0.4292141455610476
44-45	0.4041624159007054
46-47	0.4022686121794634
48-49	0.3922232185276582
50-51	0.3881680083854335
52-53	0.4499430623794244
54-55	0.4513839999934129
56-57	0.4212889886841111
58-59	0.40644733126002996
60-61	0.42083612257685754
62-63	0.40146580408024124
64-65	0.4499018927333104
66-67	0.46908694782241395
68-69	0.4136520193299722
70-71	0.3693123104652417
72-73	0.3561380237087758
74-75	0.3375499284883247
76-77	0.3359031426437664
78-79	0.3359031426437664
80-81	0.3521445680357221
82-83	0.3469777774484206
84-85	0.3472865497942753
86-87	0.33246547719325115
88-89	0.3763317351276712
90-91	0.3555822334862374
92-93	0.34570151841888797
94-95	0.35152702334401276
96-97	0.35101240276758827
98	0.3461543845261415
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	2428974.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.01285334944967
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.18336794014675	50.04740466318679
2	11.987376367905009	15.34692330966724
3	4.353017931661352	8.359472954608885
4	2.0500182546377714	5.249100715912899
5	1.076414419128083	3.445217917743952
6	0.6219022088663734	2.388584093631719
7	0.4055879552715241	1.8173989610769457
8	0.2660726876111694	1.3625657545878178
9	0.19507548539836983	1.1238604594990704
>10	0.8257359363221851	8.586302845718958
>50	0.027068295839527037	1.1443656594106597
>100	0.007716400810420467	0.8095053605351477
>500	5.815047613885583E-4	0.2593670202097078
>1k	6.461164015386013E-5	0.05993028421017034
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.1169646113955936E-5	0.0	0.0	0.0
3	0.0	4.1169646113955936E-5	0.0	0.0	0.0
4	0.0	4.1169646113955936E-5	0.0	0.0	0.0
5	0.0	4.1169646113955936E-5	0.0	0.0	0.0
6	0.0	4.1169646113955936E-5	0.0	0.0	0.0
7	8.233929222791187E-5	4.1169646113955936E-5	0.0	0.0	0.0
8	8.233929222791187E-5	4.1169646113955936E-5	0.0	0.0	0.0
9	8.233929222791187E-5	4.1169646113955936E-5	0.0	4.1169646113955936E-5	0.0
10-11	8.233929222791187E-5	4.1169646113955936E-5	0.0	4.1169646113955936E-5	0.0
12-13	1.0292411528488985E-4	4.1169646113955936E-5	0.0	4.1169646113955936E-5	0.0
14-15	1.6467858445582374E-4	4.1169646113955936E-5	0.0	6.17544691709339E-5	0.0
16-17	1.852634075128017E-4	4.1169646113955936E-5	0.0	8.233929222791187E-5	0.0
18-19	2.8818752279769156E-4	4.1169646113955936E-5	0.0	2.058482305697797E-4	0.0
20-21	3.293571689116475E-4	4.1169646113955936E-5	0.0	3.087723458546695E-4	0.0
22-23	3.293571689116475E-4	4.1169646113955936E-5	0.0	4.5286610725351527E-4	0.0
24-25	5.146205764244492E-4	4.1169646113955936E-5	0.0	5.969598686523611E-4	0.0
26-27	5.352053994814272E-4	4.1169646113955936E-5	0.0	8.645625683930746E-4	0.0
28-29	7.410536300512068E-4	4.1169646113955936E-5	0.0	0.004446321780307241	0.0
30-31	8.233929222791187E-4	4.1169646113955936E-5	0.0	0.020481898941693076	0.0
32-33	8.233929222791187E-4	4.1169646113955936E-5	0.0	0.053705803355655515	0.0
34-35	9.263170375640086E-4	4.1169646113955936E-5	0.0	0.09837486938929771	0.0
36-37	9.880715067349425E-4	4.1169646113955936E-5	0.0	0.1708540313729171	0.0
38-39	0.0010704107989628544	4.1169646113955936E-5	0.0	0.30049724698576435	0.0
40-41	0.0010909956220198323	4.1169646113955936E-5	0.0	0.45113698211672915	0.0
42-43	0.0012145045603617001	4.1169646113955936E-5	0.0	0.6098665527090863	0.0
44-45	0.0013997679678745018	4.1169646113955936E-5	0.0	0.7989175676643718	0.0
46-47	0.0015232769062163696	4.1169646113955936E-5	0.0	1.0266474651437192	0.0
48-49	0.0015644465523303255	6.17544691709339E-5	0.0	1.2797996190984342	0.0
50-51	0.0015644465523303255	8.233929222791187E-5	0.0	1.5588063108127135	0.0
52-53	0.0015644465523303255	8.233929222791187E-5	0.0	1.8483318471091086	0.0
54-55	0.0015644465523303255	8.233929222791187E-5	0.0	2.169475671621022	0.0
56-57	0.0016056161984442814	8.233929222791187E-5	0.0	2.5395907901854855	0.0
58-59	0.0016262010215012594	8.233929222791187E-5	0.0	2.9395950718286814	0.0
60-61	0.0016467858445582374	8.233929222791187E-5	0.0	3.377290164489204	0.0
62-63	0.0016467858445582374	8.233929222791187E-5	0.0	3.822848659557492	0.0
64-65	0.0016879554906721933	8.233929222791187E-5	0.0	4.290268236712291	0.0
66-67	0.001811464429014061	8.233929222791187E-5	0.0	4.8262558594698834	0.0
68-69	0.001893803721241973	8.233929222791187E-5	0.0	5.430976206414725	0.0
70-71	0.001914388544298951	8.233929222791187E-5	0.0	6.067108993344515	0.0
72-73	0.0019349733673559289	8.233929222791187E-5	0.0	6.721335839741389	0.0
74-75	0.0019967278365268628	8.233929222791187E-5	0.0	7.3715898152882655	0.0
76-77	0.002017312659583841	8.233929222791187E-5	0.0	8.080057670440276	0.0
78-79	0.002017312659583841	8.233929222791187E-5	0.0	8.857237664956479	0.0
80-81	0.00232608500543851	8.233929222791187E-5	0.0	9.677110582492855	0.0
82-83	0.002470178766837356	8.233929222791187E-5	0.0	10.523229149426879	0.0
84-85	0.002490763589894334	8.233929222791187E-5	0.0	11.362492970282926	0.0
86	0.002511348412951312	8.233929222791187E-5	0.0	12.018737129339383	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3370	0.0	40.01808	1
GGTATCA	2020	0.0	34.178932	1
TCAACGC	3880	0.0	33.80669	4
ATCAACG	3915	0.0	33.387314	3
CAACGCA	3935	0.0	33.334167	5
TATCAAC	4225	0.0	31.26647	2
AACGCAG	4370	0.0	30.432678	6
GTACATG	6875	0.0	25.039026	1
ACGCAGA	5340	0.0	24.556581	7
TACATGG	6800	0.0	24.553055	2
CGCAGAG	5440	0.0	23.681803	8
ACATGGG	7095	0.0	22.430834	3
GAGTACA	5110	0.0	21.79782	1
AGTACAT	5085	0.0	20.566336	2
GCAGAGT	6270	0.0	19.961926	9
TATACCG	140	6.147336E-4	19.655838	5
GTATAGG	660	0.0	19.52687	1
GTATTAG	645	0.0	19.26738	1
CATGGGG	5190	0.0	18.380796	4
GTGTAGC	1480	0.0	17.415857	1
>>END_MODULE