FastQCFastQC Report
Mon 15 Aug 2016
SRR2920846_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920846_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2428974
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21250.038.7069321
GTATCAA35950.038.0886151
ATCAACG42400.032.0153243
TCAACGC42950.031.4948434
CAACGCA42850.031.4530355
TATCAAC45100.030.0986632
AACGCAG46400.029.5579916
GTACATG69500.025.5167641
TACATGG69450.025.0130462
ACGCAGA56250.024.0445927
ACATGGG69450.023.7145543
CGCAGAG59550.022.469838
GAGTACA53950.020.5336461
CATGGGG51400.019.6686364
AGTACAT51150.019.486392
TATACGG1507.772251E-418.9825735
GCAGAGT67800.018.8958539
TATACAG13950.018.0300435
GTATAGC8500.017.8991581
ATACCGT3852.5465852E-1017.2568846
Produced by FastQC (version 0.11.5)