##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920846_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2428974
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.06741117854699	31.0	30.0	34.0	26.0	34.0
2	30.079310441363308	31.0	30.0	34.0	26.0	34.0
3	30.224409153823796	31.0	30.0	34.0	26.0	34.0
4	33.85291320532867	35.0	35.0	37.0	30.0	37.0
5	33.34980201517184	35.0	33.0	37.0	27.0	37.0
6	33.290299937339796	35.0	33.0	37.0	27.0	37.0
7	33.10063137769281	35.0	33.0	37.0	26.0	37.0
8	33.02754702191131	35.0	33.0	37.0	26.0	37.0
9	34.34016296592718	38.0	34.0	39.0	27.0	39.0
10-11	34.13146414906046	37.0	33.5	39.0	26.0	39.0
12-13	33.939156409249335	37.0	33.0	39.0	25.0	39.0
14-15	34.80700781482223	38.0	33.0	40.0	25.0	41.0
16-17	34.67431598691464	38.0	33.0	40.0	25.0	41.0
18-19	34.57593967658773	38.0	33.0	40.0	24.5	41.0
20-21	34.45322716504994	38.0	33.0	40.0	24.0	41.0
22-23	34.302224107792014	38.0	33.0	40.0	23.0	41.0
24-25	34.1141438730921	38.0	33.0	40.0	22.0	41.0
26-27	33.916538834915485	38.0	33.0	40.0	19.5	41.0
28-29	33.852146214821566	38.0	32.5	40.0	20.0	41.0
30-31	33.846580696211646	38.0	33.0	40.0	19.0	41.0
32-33	33.69529706781546	38.0	32.5	40.0	19.0	41.0
34-35	33.525584464881064	38.0	32.0	40.0	18.0	41.0
36-37	33.32955272473069	38.0	32.0	40.0	16.5	41.0
38-39	33.13908588564554	38.0	32.0	40.0	16.0	41.0
40-41	32.90139334550308	37.5	31.0	40.0	15.0	41.0
42-43	32.9229757090854	38.0	32.0	40.0	15.0	41.0
44-45	32.77236952721602	37.5	31.0	40.0	11.0	41.0
46-47	32.51931926813543	37.0	31.0	40.0	9.0	41.0
48-49	32.244546462827515	37.0	31.0	40.0	8.0	41.0
50-51	31.97424118166765	37.0	30.0	40.0	7.5	41.0
52-53	31.707199212507007	36.5	30.0	40.0	4.0	41.0
54-55	31.409704261964105	36.0	30.0	39.5	2.0	41.0
56-57	31.082027226310366	36.0	29.0	39.0	2.0	40.5
58-59	30.73805997923403	35.0	29.0	39.0	2.0	40.0
60-61	30.395302913905212	35.0	28.0	39.0	2.0	40.0
62-63	30.05919515812026	35.0	28.0	38.5	2.0	40.0
64-65	29.64758844680923	34.0	28.0	38.0	2.0	40.0
66-67	29.199348572689537	34.0	27.0	37.5	2.0	40.0
68-69	28.742396995603904	34.0	26.0	37.0	2.0	39.5
70-71	28.3018031481605	34.0	26.0	36.5	2.0	39.0
72-73	27.83669462909031	33.0	26.0	36.0	2.0	39.0
74-75	27.375864665492507	33.0	25.0	35.5	2.0	38.5
76-77	25.979091171828106	30.5	23.0	34.0	2.0	36.0
78-79	26.80237375945564	32.5	25.0	35.0	2.0	37.0
80-81	26.81817569887533	33.0	25.0	35.0	2.0	36.5
82-83	26.45999277884407	33.0	24.0	35.0	2.0	36.0
84-85	26.12210031890008	32.5	23.5	35.0	2.0	36.0
86-87	25.559417062512814	32.0	20.0	35.0	2.0	35.0
88-89	25.177396299836886	32.0	19.5	34.5	2.0	35.0
90-91	24.781685806435146	31.5	18.0	34.0	2.0	35.0
92-93	24.441438236885205	31.0	14.0	34.0	2.0	35.0
94-95	24.024962391528277	31.0	4.5	34.0	2.0	35.0
96-97	23.44975429955199	31.0	2.0	34.0	2.0	35.0
98-99	22.79823703341411	31.0	2.0	34.0	2.0	35.0
100-101	20.59556071822918	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	63239.0
3	28518.0
4	15490.0
5	10895.0
6	9316.0
7	9129.0
8	10300.0
9	11958.0
10	13397.0
11	15361.0
12	16859.0
13	18295.0
14	19696.0
15	20738.0
16	22113.0
17	23059.0
18	22380.0
19	21797.0
20	21935.0
21	22788.0
22	24379.0
23	27490.0
24	31647.0
25	37178.0
26	44131.0
27	51979.0
28	61258.0
29	72021.0
30	83602.0
31	98760.0
32	118096.0
33	143055.0
34	179329.0
35	229089.0
36	296917.0
37	338307.0
38	182494.0
39	11979.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.906870553955585	18.90522227437833	12.809186477507748	25.37872069415834
2	16.586921062143933	20.237474752714522	37.56219704286666	25.613407142274884
3	20.32566688544138	23.208181570385207	28.46375682572988	28.00239471844353
4	14.019081030556826	15.590692541762916	35.242877554465764	35.147348873214504
5	14.581948061305363	36.75011579673299	34.11592024950336	14.552015892458286
6	34.95363863938535	34.71740135692189	16.181058649655824	14.147901354036929
7	30.360426399990764	30.797116984592144	19.888757691146957	18.953698924270135
8	27.24765752430276	35.015843219114586	19.034493163322217	18.70200609326044
9	26.699399382561317	15.530916461073657	18.81056473211341	38.95911942425162
10-11	26.247307010490033	25.30136546469448	26.97661411412959	21.474713410685897
12-13	26.687338262724502	22.15551055139621	27.150326509137457	24.006824676741836
14-15	23.370200165734847	23.946212220976772	24.473732098544843	28.209855514743538
16-17	23.93098415187211	27.098982464459176	25.2843132186104	23.685720165058317
18-19	23.83994182249681	26.42904672535233	26.571462121789573	23.15954933036128
20-21	24.367539490621485	25.61513197792574	26.588371494884942	23.428957036567834
22-23	24.006904121871184	25.564019290007728	26.43465724141682	23.994419346704266
24-25	23.83419571728636	25.7496273026599	26.548902957810128	23.867274022243613
26-27	23.750275501103037	26.02895284862991	26.410246375229413	23.810525275037644
28-29	23.72074633212624	26.110009956723772	26.397261952444545	23.77198175870544
30-31	24.00839074966794	25.82408045500133	26.342057706170163	23.82547108916057
32-33	23.73227988135281	25.852156374609663	26.374414048222818	24.041149695814713
34-35	23.982579237272343	25.92189810793288	26.31751569859602	23.77800695619876
36-37	24.053456839081385	25.80093222296757	26.316273875396405	23.82933706255464
38-39	23.889625481344694	25.859666542227274	26.378873279168907	23.87183469725913
40-41	24.037987769937782	25.766108450536894	26.364228046041767	23.83167573348356
42-43	23.91693047763493	25.725867035154504	26.314997398776157	24.042205088434404
44-45	23.841323865970306	25.802641101903873	26.375315673139188	23.980719358986637
46-47	23.847706615088697	25.76258608082594	26.253594015407085	24.136113288678278
48-49	23.762495069496424	25.862745485552697	26.326212821532653	24.048546623418225
50-51	23.77735279821182	25.91703043732566	26.311247137512822	23.994369626949698
52-53	23.90810678947092	25.771600967145265	26.260690732759727	24.059601510624084
54-55	23.590711087769677	25.607341105592113	26.531320196295887	24.270627610342324
56-57	23.567609451466858	25.818015559212064	26.351168999491737	24.263205989829345
58-59	23.549190863630486	25.758825239224898	26.39761602644175	24.294367870702864
60-61	23.623038460231818	25.698464123240754	26.463900873717144	24.214596542810288
62-63	23.52769579066967	25.696437508324806	26.45263347943817	24.323233221567357
64-65	23.298415577223754	25.592056167596994	26.46044707216828	24.64908118301097
66-67	23.299638116376485	25.707122824409733	26.317771836421933	24.675467222791852
68-69	23.388280795762988	25.560109329022584	26.3467615624342	24.704848312780232
70-71	23.275592632810405	25.378277357137886	26.331418937977958	25.01471107207375
72-73	23.347859673127495	25.423119719048053	26.20696955399723	25.022051053827226
74-75	23.238196838205738	25.521126598064225	26.080383405617457	25.160293158112584
76-77	23.194240637496897	25.216195864879413	26.382963268525305	25.206600229098385
78-79	23.116211959090123	25.26199058873359	26.40352174681543	25.21827570536086
80-81	23.154425078930114	25.317403536439613	26.229853133888327	25.298318250741946
82-83	23.149939422262346	25.320526743244066	26.18942869583388	25.340105138659712
84-85	23.065766163607858	25.189936353333188	26.18044854275588	25.563848940303068
86-87	22.978620032473756	25.09944494930284	26.26269234984396	25.659242668379445
88-89	22.918708245378447	25.07940019375449	26.2132596096864	25.788631951180662
90-91	22.910666898659414	25.057404789577003	26.15022686372841	25.881701448035173
92-93	22.956731116833712	24.938017032560012	26.18104356139402	25.92420828921226
94-95	22.747735276129884	24.776358067526203	26.29568430818648	26.180222348157432
96-97	22.82779579832296	24.559716921103224	26.336432656672553	26.276054623901263
98-99	22.707930757713964	24.548626400150027	26.236598053062476	26.506844789073536
100-101	22.849546092425914	24.40019540083418	26.27684213971736	26.473416367022544
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	23.0
1	15.0
2	21.5
3	45.0
4	40.5
5	47.5
6	101.0
7	182.5
8	271.5
9	309.5
10	269.5
11	227.0
12	267.5
13	336.0
14	354.5
15	364.0
16	430.0
17	509.5
18	583.0
19	660.0
20	779.5
21	978.0
22	1224.0
23	1602.5
24	2056.0
25	2615.0
26	3536.0
27	4769.0
28	6364.5
29	8536.0
30	11554.5
31	15548.0
32	20788.5
33	25662.0
34	29837.0
35	34924.5
36	41262.5
37	47366.0
38	52348.5
39	57414.0
40	63130.0
41	68979.0
42	73485.5
43	78036.5
44	83154.5
45	88173.5
46	93148.5
47	97761.0
48	102404.5
49	105485.0
50	106256.0
51	106713.5
52	107094.0
53	107009.0
54	104826.0
55	99341.5
56	92184.5
57	84035.0
58	75796.5
59	66358.0
60	55389.5
61	45751.5
62	37167.0
63	28592.5
64	21246.5
65	15774.0
66	11978.0
67	9353.0
68	6996.0
69	4976.0
70	3723.5
71	2785.0
72	2090.5
73	1716.5
74	1295.0
75	899.0
76	651.0
77	415.0
78	252.5
79	159.0
80	82.5
81	43.5
82	21.5
83	15.0
84	10.0
85	2.0
86	1.0
87	1.0
88	1.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19053312221538807
2	0.0
3	0.012186215249730957
4	0.015809144107759077
5	0.006134277270979434
6	0.10704107989628543
7	0.14236463626205964
8	0.12412648303357715
9	0.1428586720154271
10-11	0.11391641079731606
12-13	0.11438986172762657
14-15	0.1156661207571592
16-17	0.06543915249813297
18-19	0.07900455089268144
20-21	0.03694975738727545
22-23	0.08308034585796308
24-25	0.05767867420565226
26-27	0.06500687121393642
28-29	0.03979046296913841
30-31	0.0241048277997212
32-33	0.031556533746347225
34-35	0.020276050711123296
36-37	0.02408424297666422
38-39	0.030897819408523926
40-41	0.025257577890911966
42-43	0.01274200547226936
44-45	0.02599863152096317
46-47	0.033162149944791504
48-49	0.008892643560614482
50-51	0.014841657424081113
52-53	0.0072664425391132225
54-55	0.012083291134446067
56-57	0.005084451295073558
58-59	0.003622928858028122
60-61	0.02291090806241648
62-63	0.00981896059817849
64-65	0.007060594308543443
66-67	0.04049034695307566
68-69	0.08937930171339833
70-71	0.0218610820865106
72-73	0.04468965085669917
74-75	0.08614748449345279
76-77	0.24691495256845072
78-79	0.07752244363257901
80-81	0.016529612914753307
82-83	0.011383407150508815
84-85	0.013647737686776391
86-87	0.26358865924460284
88-89	0.6214352232671079
90-91	0.2669645702259472
92-93	0.3520416439204372
94-95	0.0014615224370454357
96-97	0.00454924589559213
98-99	0.0033964958044013643
100-101	0.0049609423567316905
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2428974.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.28433544225284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.70232909317177	47.77353594441179
2	12.800507386953619	15.94542191840109
3	4.66254430840378	8.712104211569633
4	2.1547974723841166	5.368405143203635
5	1.1432047854922835	3.5601875169395045
6	0.685381724608391	2.5613127144899264
7	0.4183608778977639	1.8240130478429792
8	0.28354106189741884	1.4128133288697127
9	0.20572498253093918	1.1532099438727759
>10	0.9046649206549792	9.258649600008475
>50	0.030237567349906107	1.261109245365061
>100	0.007910578796728438	0.8230157922395277
>500	7.289698701268065E-4	0.2928932310936788
>1k	6.626998819224091E-5	0.053328361692210106
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	4.1169646113955936E-5	8.233929222791187E-5	0.0	0.0	0.0
3	4.1169646113955936E-5	8.233929222791187E-5	0.0	0.0	0.0
4	4.1169646113955936E-5	1.235089383418678E-4	0.0	0.0	0.0
5	1.235089383418678E-4	1.6467858445582374E-4	0.0	0.0	0.0
6	1.235089383418678E-4	1.6467858445582374E-4	0.0	0.0	0.0
7	1.6467858445582374E-4	1.6467858445582374E-4	0.0	0.0	0.0
8	2.0584823056977967E-4	1.6467858445582374E-4	0.0	0.0	0.0
9	2.0584823056977967E-4	1.6467858445582374E-4	0.0	0.0	0.0
10-11	2.0584823056977967E-4	1.6467858445582374E-4	0.0	0.0	0.0
12-13	2.676026997407136E-4	1.6467858445582374E-4	0.0	0.0	0.0
14-15	3.087723458546695E-4	1.6467858445582374E-4	0.0	2.0584823056977968E-5	0.0
16-17	3.4994199196862545E-4	1.6467858445582374E-4	0.0	4.1169646113955936E-5	0.0
18-19	4.1169646113955934E-4	2.0584823056977967E-4	0.0	1.4409376139884578E-4	0.0
20-21	4.5286610725351527E-4	2.0584823056977967E-4	0.0	2.2643305362675766E-4	0.0
22-23	4.5286610725351527E-4	2.0584823056977967E-4	0.0	3.911116380825814E-4	0.0
24-25	7.410536300512068E-4	2.0584823056977967E-4	0.0	7.616384531081848E-4	0.0
26-27	7.410536300512068E-4	2.0584823056977967E-4	0.0	0.0010498259759058764	0.0
28-29	9.469018606209866E-4	2.0584823056977967E-4	0.0	0.0044669066033642185	0.0
30-31	0.0011115804450768103	2.0584823056977967E-4	0.0	0.02025546588806632	0.0
32-33	0.0011321652681337883	2.0584823056977967E-4	0.0	0.05339703100980085	0.0
34-35	0.0011939197373047222	3.911116380825814E-4	0.0	0.09786024881287325	0.0
36-37	0.00131742867564659	4.1169646113955934E-4	0.0	0.1696601116356124	0.0
38-39	0.0015232769062163696	4.1169646113955934E-4	0.0	0.298027068218927	0.0
40-41	0.0015232769062163696	4.1169646113955934E-4	0.0	0.44718469608978934	0.0
42-43	0.0015850313753873035	4.322812841965373E-4	0.0	0.6050908737598673	0.0
44-45	0.0016879554906721933	4.5286610725351527E-4	0.0	0.7925774421628227	0.0
46-47	0.0018938037212419732	4.5286610725351527E-4	0.0	1.0178371608753327	0.0
48-49	0.002017312659583841	4.734509303104933E-4	0.0	1.2671811225645067	0.0
50-51	0.002017312659583841	5.146205764244492E-4	0.0	1.5405475727611742	0.0
52-53	0.002017312659583841	5.352053994814272E-4	0.0	1.8239800014327037	0.0
54-55	0.002017312659583841	5.352053994814272E-4	0.0	2.139401245134777	0.0
56-57	0.0022231608901536206	6.58714337823295E-4	0.0	2.5013236041225637	0.0
58-59	0.0022231608901536206	6.792991608802729E-4	0.0	2.8929292779585127	4.1169646113955936E-5
60-61	0.0022643305362675765	7.822232761651628E-4	0.0	3.3219787449351044	4.1169646113955936E-5
62-63	0.0022643305362675765	7.822232761651628E-4	0.0	3.7591180473730885	4.1169646113955936E-5
64-65	0.0022643305362675765	7.822232761651628E-4	0.0	4.2179949229592415	4.1169646113955936E-5
66-67	0.0023466698284954884	8.233929222791187E-4	0.0	4.741302294713735	4.1169646113955936E-5
68-69	0.0023466698284954884	8.645625683930746E-4	0.0	5.328463787590975	4.1169646113955936E-5
70-71	0.002408424297666422	9.263170375640086E-4	0.0	5.948787430413006	4.1169646113955936E-5
72-73	0.0024290091207234002	9.880715067349425E-4	0.0	6.589366539123103	4.1169646113955936E-5
74-75	0.0024290091207234002	9.880715067349425E-4	0.0	7.231118982747448	4.1169646113955936E-5
76-77	0.0024495939437803784	9.880715067349425E-4	0.0	7.927585886057241	4.1169646113955936E-5
78-79	0.002470178766837356	9.880715067349425E-4	0.0	8.690418258902731	4.1169646113955936E-5
80-81	0.00263485735129318	9.880715067349425E-4	0.0	9.494337938569949	4.1169646113955936E-5
82-83	0.00263485735129318	9.880715067349425E-4	0.0	10.326211807948543	4.1169646113955936E-5
84-85	0.002696611820464114	9.880715067349425E-4	0.0	11.149501806112376	4.1169646113955936E-5
86-87	0.0027583662896350477	9.880715067349425E-4	0.0	12.014599579904932	4.1169646113955936E-5
88-89	0.0027583662896350477	9.880715067349425E-4	2.0584823056977968E-5	12.931756371208586	4.1169646113955936E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2125	0.0	38.706932	1
GTATCAA	3595	0.0	38.088615	1
ATCAACG	4240	0.0	32.015324	3
TCAACGC	4295	0.0	31.494843	4
CAACGCA	4285	0.0	31.453035	5
TATCAAC	4510	0.0	30.098663	2
AACGCAG	4640	0.0	29.557991	6
GTACATG	6950	0.0	25.516764	1
TACATGG	6945	0.0	25.013046	2
ACGCAGA	5625	0.0	24.044592	7
ACATGGG	6945	0.0	23.714554	3
CGCAGAG	5955	0.0	22.46983	8
GAGTACA	5395	0.0	20.533646	1
CATGGGG	5140	0.0	19.668636	4
AGTACAT	5115	0.0	19.48639	2
TATACGG	150	7.772251E-4	18.982573	5
GCAGAGT	6780	0.0	18.895853	9
TATACAG	1395	0.0	18.030043	5
GTATAGC	850	0.0	17.899158	1
ATACCGT	385	2.5465852E-10	17.256884	6
>>END_MODULE