FastQCFastQC Report
Mon 15 Aug 2016
SRR2920845_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920845_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1384510
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG26650.19248687261197103No Hit
TCCATGTACTCTGCGTTGATACCAC26390.19060895190356153No Hit
TTGTAGAACAGTGTATATCAATGAG14120.10198554001054526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT804.311005E-1016.6244321
CGTTATT852.6994167E-713.4113052
AACCGCG1153.765308E-1013.2169397
TCCAACG2050.012.97610218
CAAGACG1301.9826984E-1012.4226514
CTCGTAG1001.4419311E-712.35002310
GGTCGGC853.9423503E-612.29414211
CCAACGA1405.2750693E-1112.2147519
GTCGGCG701.0903475E-412.2147512
CGGACAT701.09108296E-412.2138685
TCCCGGA550.003067281312.09136917
AGAACCG1353.7107384E-1011.9625545
CGAGCCG2650.011.83063815
GCTCGTA1052.720044E-711.7615029
CCGGAAG658.019894E-411.69275219
CCGATAA658.0247555E-411.6919079
GAACCGC1302.6138878E-911.6919076
GAGCCGC2850.011.6671116
GTATTAG3600.011.6107141
CGCAAGA1406.8030204E-1011.5353192
Produced by FastQC (version 0.11.5)