##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920845_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1384510
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.3422712728691	32.0	32.0	33.0	18.0	34.0
2	30.544223588128652	32.0	32.0	33.0	18.0	34.0
3	30.91343507811428	32.0	32.0	33.0	29.0	34.0
4	31.053873211461095	32.0	32.0	33.0	29.0	34.0
5	31.035867563253426	32.0	32.0	33.0	29.0	34.0
6	34.003773176069515	37.0	33.0	37.0	29.0	37.0
7	34.04273497482864	37.0	33.0	37.0	29.0	37.0
8	34.0812857978635	37.0	33.0	37.0	29.0	37.0
9	34.09971397823057	37.0	33.0	37.0	30.0	37.0
10	33.83587334147099	37.0	33.0	37.0	29.0	37.0
11	33.72858484229077	37.0	33.0	37.0	29.0	37.0
12	34.05264606250587	37.0	33.0	37.0	30.0	37.0
13	35.585311048674264	38.0	37.0	38.0	32.0	38.0
14	35.084278192284636	38.0	36.0	38.0	20.0	38.0
15	35.191546467703375	38.0	36.0	38.0	30.0	38.0
16	35.40927115008198	38.0	37.0	38.0	32.0	38.0
17	35.38040823107092	38.0	37.0	38.0	32.0	38.0
18	35.424010660811405	38.0	37.0	38.0	32.0	38.0
19	35.26270593928538	38.0	37.0	38.0	32.0	38.0
20	35.433939805418525	38.0	37.0	38.0	32.0	38.0
21	35.57537757040397	38.0	37.0	38.0	32.0	38.0
22	35.474520227372864	38.0	37.0	38.0	32.0	38.0
23	35.685677965489596	38.0	37.0	39.0	32.0	39.0
24	35.68129518746704	38.0	37.0	39.0	32.0	39.0
25	35.677007750034306	38.0	37.0	39.0	32.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	7.0
15	140.0
16	319.0
17	521.0
18	1262.0
19	2506.0
20	4503.0
21	7162.0
22	10110.0
23	13222.0
24	16261.0
25	19842.0
26	23239.0
27	26797.0
28	30633.0
29	35501.0
30	40628.0
31	47691.0
32	57840.0
33	76931.0
34	118500.0
35	238259.0
36	559266.0
37	53369.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.25921083993615	19.790828524171005	13.283327675495302	24.666632960397543
2	14.235433474658905	21.724436804356774	41.02187777625297	23.01825194473135
3	17.8077442560906	26.64169995160743	30.05640984897184	25.494145943330132
4	11.5352724068443	17.701425052906806	39.77710525745571	30.986197282793192
5	13.091635307798427	37.88091093599902	35.145647196481065	13.881806559721491
6	29.53189214956916	38.62196733862522	19.197333352594057	12.648807159211561
7	26.59337960722566	32.37441405262512	23.245697033607556	17.786509306541664
8	24.956988393005467	35.54932792106955	22.01392550433005	17.47975818159493
9	26.35936179587002	16.026536464164217	20.912669464287003	36.701432275678755
10	14.181334912712801	29.474615567962676	33.842297997125335	22.50175152219919
11	34.01651125669009	23.45010147994597	24.264902384237022	18.268484879126913
12	22.308325689233012	25.750120981430253	31.678066608402972	20.26348672093376
13	27.617496442784812	21.962571595727006	26.83281449754787	23.58711746394031
14	21.500747556897387	22.493084195852685	27.721070992625553	28.285097254624382
15	22.953063470922523	29.89401289122201	24.24464141774551	22.90828222010996
16	22.691359005565165	28.698704591171577	26.763861452288	21.846074950975257
17	21.447825358395498	28.382149852943726	27.862252474532394	22.307772314128382
18	21.869054652773663	27.975002997502322	29.144895650804703	21.011046698919312
19	23.433092932802214	27.573375129559874	27.217505299766344	21.77602663787157
20	23.12366602985204	27.354233854221217	27.752212900732758	21.769887215193986
21	24.497220665306855	27.081178530361775	26.081975927841306	22.339624876490063
22	22.580151216172098	27.726889263189523	27.391896615982	22.301062904656384
23	22.22732634021017	27.100338533033153	27.09607707603378	23.576258050722895
24	22.124265171645934	28.121779088151953	27.474617318655664	22.279338421546445
25	22.904541298120467	27.22698689918483	27.188850222536175	22.679621580158525
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	79.0
1	79.0
2	126.0
3	173.0
4	173.0
5	173.0
6	461.5
7	750.0
8	750.0
9	750.0
10	1580.0
11	2410.0
12	2410.0
13	2410.0
14	4582.5
15	6755.0
16	6755.0
17	6755.0
18	12258.0
19	17761.0
20	17761.0
21	17761.0
22	28784.0
23	39807.0
24	39807.0
25	39807.0
26	56412.0
27	73017.0
28	73017.0
29	73017.0
30	92283.0
31	111549.0
32	111549.0
33	111549.0
34	126710.5
35	141872.0
36	141872.0
37	141872.0
38	149148.0
39	156424.0
40	156424.0
41	156424.0
42	163978.5
43	171533.0
44	171533.0
45	171533.0
46	178942.5
47	186352.0
48	186352.0
49	186352.0
50	171271.5
51	156191.0
52	156191.0
53	156191.0
54	134033.5
55	111876.0
56	111876.0
57	111876.0
58	96036.0
59	80196.0
60	80196.0
61	80196.0
62	68191.5
63	56187.0
64	56187.0
65	56187.0
66	44450.0
67	32713.0
68	32713.0
69	32713.0
70	24328.5
71	15944.0
72	15944.0
73	15944.0
74	12648.0
75	9352.0
76	9352.0
77	9352.0
78	8605.5
79	7859.0
80	7859.0
81	7859.0
82	5595.5
83	3332.0
84	3332.0
85	3332.0
86	2532.0
87	1732.0
88	1732.0
89	1732.0
90	1163.0
91	594.0
92	594.0
93	594.0
94	316.5
95	39.0
96	39.0
97	39.0
98	26.0
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.4445543910842102E-4
16	3.6113859777105254E-4
17	4.333663173252631E-4
18	0.0017334652693010524
19	0.0010834157933131577
20	0.0010834157933131577
21	4.333663173252631E-4
22	1.4445543910842102E-4
23	5.055940368794736E-4
24	2.1668315866263155E-4
25	0.0011556435128673682
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1384510.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.35507106959794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.01253768149792	71.39019062880229
2	2.2797447598172966	3.3902116727351714
3	0.5881156376599832	1.311881401060499
4	0.2747230829961358	0.8170821744254693
5	0.15418506720880434	0.5732220815090691
6	0.09962655272537835	0.4444643644988732
7	0.07527674612535162	0.39180454656270214
8	0.048670048306173934	0.2895091920613062
9	0.0407785188399677	0.2728880699812863
>10	0.30756451805341317	4.685721968156959
>50	0.054180737669081	2.8611917341922424
>100	0.05668092340734138	8.702433069660731
>500	0.006352125556181724	3.2596845904886704
>1k	0.0015636001369062705	1.6097145058648132
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	2665	0.19248687261197103	No Hit
TCCATGTACTCTGCGTTGATACCAC	2639	0.19060895190356153	No Hit
TTGTAGAACAGTGTATATCAATGAG	1412	0.10198554001054526	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	7.222771955421051E-5	0.0
6	0.0	0.0	0.0	7.222771955421051E-5	0.0
7	0.0	0.0	0.0	7.222771955421051E-5	0.0
8	0.0	0.0	0.0	1.4445543910842102E-4	0.0
9	7.222771955421051E-5	0.0	0.0	1.4445543910842102E-4	0.0
10	7.222771955421051E-5	0.0	0.0	1.4445543910842102E-4	0.0
11	7.222771955421051E-5	0.0	0.0	1.4445543910842102E-4	0.0
12	7.222771955421051E-5	0.0	0.0	1.4445543910842102E-4	7.222771955421051E-5
13	7.222771955421051E-5	0.0	0.0	1.4445543910842102E-4	7.222771955421051E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	80	4.311005E-10	16.624432	1
CGTTATT	85	2.6994167E-7	13.411305	2
AACCGCG	115	3.765308E-10	13.216939	7
TCCAACG	205	0.0	12.976102	18
CAAGACG	130	1.9826984E-10	12.422651	4
CTCGTAG	100	1.4419311E-7	12.350023	10
GGTCGGC	85	3.9423503E-6	12.294142	11
CCAACGA	140	5.2750693E-11	12.21475	19
GTCGGCG	70	1.0903475E-4	12.21475	12
CGGACAT	70	1.09108296E-4	12.213868	5
TCCCGGA	55	0.0030672813	12.091369	17
AGAACCG	135	3.7107384E-10	11.962554	5
CGAGCCG	265	0.0	11.830638	15
GCTCGTA	105	2.720044E-7	11.761502	9
CCGGAAG	65	8.019894E-4	11.692752	19
CCGATAA	65	8.0247555E-4	11.691907	9
GAACCGC	130	2.6138878E-9	11.691907	6
GAGCCGC	285	0.0	11.66711	16
GTATTAG	360	0.0	11.610714	1
CGCAAGA	140	6.8030204E-10	11.535319	2
>>END_MODULE