FastQCFastQC Report
Mon 15 Aug 2016
SRR2920845_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920845_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1384510
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCAC27600.19934850596962103No Hit
GTACATGGAAGCAGTGGTATCAACG26900.19429256560082628No Hit
GTATCAACGCAGAGTACTTTTTTTT20510.1481390528056858No Hit
TTGTAGAACAGTGTATATCAATGAG14870.10740261897711104No Hit
GAATAGGACCGCGGTTCTATTTTGT13930.10061321333901525No Hit
CTATTGGAGCTGGAATTACCGCGGC13850.10003539158258157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGT807.712515E-915.437519
CGACCAT1500.013.93333410
TCCAACG2200.013.81818218
GCTCGTA551.9599091E-413.8181829
CCGACCA1550.013.48387059
CGAACGA500.001499403213.29999916
ATCGCCA2200.012.95454616
CCAACGA1403.6379788E-1212.89285819
CGCCAGT2350.012.53191618
CAAGACG1900.012.54
TAAACGC1750.012.48571518
GTAAACG1950.012.17948717
CGTCACT1251.4097168E-912.1615
CGCCCGA951.0394888E-612.016
CGCATCG2300.011.97826113
CGCAAGA1850.011.810812
CGGTTCT3150.011.76190512
CTCGTAG658.0224517E-411.69230710
CGTCGTA1302.6138878E-911.69230710
CATCGCC2450.011.63265215
Produced by FastQC (version 0.11.5)