##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920845_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1384510
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.392822009230706	33.0	32.0	34.0	32.0	34.0
2	32.42922550216322	33.0	32.0	34.0	32.0	34.0
3	32.64730410036764	33.0	32.0	34.0	32.0	34.0
4	32.793860643837895	33.0	32.0	34.0	32.0	34.0
5	32.662092725946366	33.0	32.0	34.0	32.0	34.0
6	35.74545651530144	37.0	37.0	37.0	33.0	37.0
7	35.70027374305711	37.0	37.0	37.0	33.0	37.0
8	35.521981061891935	37.0	35.0	37.0	33.0	37.0
9	35.562639489783386	37.0	35.0	37.0	33.0	37.0
10	35.46924038107345	37.0	35.0	37.0	33.0	37.0
11	35.49462553538797	37.0	34.0	37.0	33.0	37.0
12	35.39866378718825	37.0	34.0	37.0	33.0	37.0
13	37.09902131440004	38.0	38.0	38.0	36.0	38.0
14	37.046222129128715	38.0	38.0	38.0	36.0	38.0
15	36.99725968032011	38.0	38.0	38.0	36.0	38.0
16	37.08203046565211	38.0	38.0	38.0	36.0	38.0
17	37.05611299304447	38.0	38.0	38.0	36.0	38.0
18	37.1054120230262	38.0	38.0	38.0	36.0	38.0
19	37.01866725411879	38.0	38.0	38.0	36.0	38.0
20	37.06440473524929	38.0	38.0	38.0	36.0	38.0
21	37.16669074257319	38.0	38.0	38.0	37.0	38.0
22	37.11545312059862	38.0	38.0	38.0	36.0	38.0
23	37.650055976482655	39.0	38.0	39.0	36.0	39.0
24	37.653462958014025	39.0	38.0	39.0	36.0	39.0
25	37.51298148803548	39.0	38.0	39.0	36.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	19.0
16	58.0
17	26.0
18	21.0
19	15.0
20	21.0
21	35.0
22	63.0
23	212.0
24	511.0
25	1217.0
26	2443.0
27	4743.0
28	7812.0
29	12352.0
30	17963.0
31	25290.0
32	34963.0
33	50079.0
34	82607.0
35	198633.0
36	773601.0
37	171824.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.36929310730872	20.472441513604092	13.279644061798038	24.87862131728915
2	14.445182772244333	22.168637279615172	40.41682616954735	22.96935377859315
3	17.799654751500533	26.809123805534085	30.075044600616824	25.316176842348558
4	11.838556601252428	18.032661374782414	39.34323334609356	30.785548677871592
5	12.96184209575951	38.15703750785476	35.065835566373664	13.815284830012061
6	29.50011195296531	39.085163704126366	18.890004405890892	12.524719937017428
7	26.386374962983293	32.58156315230659	23.44143415359947	17.590627731110644
8	24.842363002072936	36.018085820976374	21.796158929874107	17.343392247076583
9	26.179948140497363	16.04148760211194	21.0876050010473	36.6909592563434
10	14.228932979899026	29.77132703989137	33.680580133043456	22.319159847166144
11	34.01961704863092	23.576861127763614	24.17273981408585	18.230782009519615
12	22.341405984788842	25.98124968400373	31.706596557626888	19.970747773580545
13	27.699402676759288	22.06072906660118	26.88091815877097	23.35895009786856
14	21.55311265357419	22.47242706806018	27.885533510050486	28.088926768315144
15	22.884430509299687	29.943972917474714	24.407136392757288	22.76446018046831
16	22.580272371714077	28.83326531865179	26.726808498344173	21.859653811289956
17	21.213209005352073	28.423630020729355	28.00947627680551	22.353684697113057
18	21.69482343933955	28.06386374962983	29.288701417830136	20.95261139320048
19	23.279644061798034	27.553502683259783	27.629197333352597	21.53765592158959
20	23.01088471733682	27.392507096373446	28.02471632563145	21.571891860658283
21	24.357715003864183	27.074994041213134	26.402698427602544	22.164592527320135
22	22.476977414392096	27.643137283226555	27.67376183631754	22.206123466063808
23	22.051845057096013	26.93248875053268	27.46235130118237	23.55331489118894
24	22.06722956136106	28.11305082664625	27.76072401066081	22.05899560133188
25	22.753176213967397	27.155961314833405	27.474052191750147	22.616810279449048
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	85.0
1	85.0
2	173.5
3	262.0
4	262.0
5	262.0
6	759.0
7	1256.0
8	1256.0
9	1256.0
10	1998.5
11	2741.0
12	2741.0
13	2741.0
14	4736.5
15	6732.0
16	6732.0
17	6732.0
18	12489.5
19	18247.0
20	18247.0
21	18247.0
22	29667.0
23	41087.0
24	41087.0
25	41087.0
26	58514.5
27	75942.0
28	75942.0
29	75942.0
30	95146.0
31	114350.0
32	114350.0
33	114350.0
34	128500.0
35	142650.0
36	142650.0
37	142650.0
38	149540.5
39	156431.0
40	156431.0
41	156431.0
42	163432.5
43	170434.0
44	170434.0
45	170434.0
46	177963.5
47	185493.0
48	185493.0
49	185493.0
50	170393.0
51	155293.0
52	155293.0
53	155293.0
54	132333.0
55	109373.0
56	109373.0
57	109373.0
58	94254.5
59	79136.0
60	79136.0
61	79136.0
62	67114.0
63	55092.0
64	55092.0
65	55092.0
66	43732.0
67	32372.0
68	32372.0
69	32372.0
70	23735.0
71	15098.0
72	15098.0
73	15098.0
74	12077.5
75	9057.0
76	9057.0
77	9057.0
78	8465.5
79	7874.0
80	7874.0
81	7874.0
82	5570.5
83	3267.0
84	3267.0
85	3267.0
86	2439.0
87	1611.0
88	1611.0
89	1611.0
90	1103.0
91	595.0
92	595.0
93	595.0
94	313.0
95	31.0
96	31.0
97	31.0
98	16.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	7.222771955421051E-5
16	3.6113859777105254E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1384510.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.33411478363925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.8353662599045	69.3216638337604
2	2.4171582182508042	3.496860000183412
3	0.5608861311007313	1.217136053627749
4	0.28741363838135814	0.8315924443624216
5	0.15734891465129028	0.5690847226733743
6	0.09878105120710023	0.4287143937877761
7	0.07446528803930294	0.37704664847021885
8	0.06105127613764376	0.35328720126623836
9	0.04324144180452575	0.28150482734087595
>10	0.3342809968428563	4.887360336710475
>50	0.05788661242987039	2.977065942258692
>100	0.062444352585359075	9.328940699382436
>500	0.0074813030912684216	3.7201051841162394
>1k	0.002194515573438737	2.209637712059733
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCAC	2760	0.19934850596962103	No Hit
GTACATGGAAGCAGTGGTATCAACG	2690	0.19429256560082628	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2051	0.1481390528056858	No Hit
TTGTAGAACAGTGTATATCAATGAG	1487	0.10740261897711104	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1393	0.10061321333901525	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1385	0.10003539158258157	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.222771955421051E-5
2	0.0	0.0	0.0	0.0	7.222771955421051E-5
3	0.0	0.0	0.0	0.0	7.222771955421051E-5
4	0.0	0.0	0.0	0.0	7.222771955421051E-5
5	0.0	0.0	0.0	0.0	7.222771955421051E-5
6	0.0	0.0	0.0	0.0	7.222771955421051E-5
7	0.0	0.0	0.0	0.0	7.222771955421051E-5
8	0.0	0.0	0.0	7.222771955421051E-5	7.222771955421051E-5
9	0.0	0.0	0.0	7.222771955421051E-5	7.222771955421051E-5
10	0.0	0.0	0.0	7.222771955421051E-5	7.222771955421051E-5
11	0.0	0.0	0.0	1.4445543910842102E-4	7.222771955421051E-5
12	0.0	0.0	0.0	1.4445543910842102E-4	7.222771955421051E-5
13	0.0	0.0	0.0	1.4445543910842102E-4	7.222771955421051E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAACGT	80	7.712515E-9	15.4375	19
CGACCAT	150	0.0	13.933334	10
TCCAACG	220	0.0	13.818182	18
GCTCGTA	55	1.9599091E-4	13.818182	9
CCGACCA	155	0.0	13.4838705	9
CGAACGA	50	0.0014994032	13.299999	16
ATCGCCA	220	0.0	12.954546	16
CCAACGA	140	3.6379788E-12	12.892858	19
CGCCAGT	235	0.0	12.531916	18
CAAGACG	190	0.0	12.5	4
TAAACGC	175	0.0	12.485715	18
GTAAACG	195	0.0	12.179487	17
CGTCACT	125	1.4097168E-9	12.16	15
CGCCCGA	95	1.0394888E-6	12.0	16
CGCATCG	230	0.0	11.978261	13
CGCAAGA	185	0.0	11.81081	2
CGGTTCT	315	0.0	11.761905	12
CTCGTAG	65	8.0224517E-4	11.692307	10
CGTCGTA	130	2.6138878E-9	11.692307	10
CATCGCC	245	0.0	11.632652	15
>>END_MODULE