FastQCFastQC Report
Mon 15 Aug 2016
SRR2920844_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920844_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1565004
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG22190.14178877498076684No Hit
TCCATGTACTCTGCGTTGATACCAC19750.12619776051690604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG1200.017.41666813
GCATCGC1250.016.7214
ATCGCCA1350.015.48148116
CATCGCC1500.014.56666715
CCAACGA1006.6029315E-1014.25000119
TCGCCAG1500.013.93333217
CGCCAGT1500.013.93333218
AGGCCCG1650.013.24242410
GCTCGTA655.4525368E-513.1538469
CGAGCCG1600.013.062515
CCGCATC1600.013.062512
AACCGCG905.405036E-712.6666667
TCCAACG1501.8189894E-1212.66666618
CGGTTCT1600.012.4687512
CGAGGTT550.003068462512.0909094
TACACCG550.003068462512.0909095
GAGCCGC1800.011.61111116
CGCGGTC1001.9294166E-611.40000110
AAAGGCG752.0738333E-411.45
GCCGCCT1850.011.297297518
Produced by FastQC (version 0.11.5)