##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920844_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1565004
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.225182172058346	32.0	32.0	33.0	29.0	34.0
2	31.445831448354127	33.0	32.0	33.0	29.0	34.0
3	31.79205740049227	33.0	32.0	33.0	30.0	34.0
4	31.907549757061325	33.0	32.0	34.0	30.0	34.0
5	31.903831555702094	33.0	32.0	33.0	30.0	34.0
6	34.96688826354438	37.0	35.0	37.0	32.0	37.0
7	34.98582815123795	37.0	35.0	37.0	32.0	37.0
8	35.01678462163675	37.0	35.0	37.0	32.0	37.0
9	35.03831491804494	37.0	34.0	37.0	32.0	37.0
10	34.82284326429836	37.0	34.0	37.0	32.0	37.0
11	34.72108825280958	37.0	34.0	37.0	32.0	37.0
12	34.96283843363979	37.0	34.0	37.0	32.0	37.0
13	36.52635967703597	38.0	38.0	38.0	34.0	38.0
14	36.1689216129799	38.0	37.0	38.0	33.0	38.0
15	36.259107963941304	38.0	37.0	38.0	33.0	38.0
16	36.414894786211406	38.0	37.0	38.0	34.0	38.0
17	36.40088395940202	38.0	38.0	38.0	34.0	38.0
18	36.43014906032189	38.0	38.0	38.0	34.0	38.0
19	36.31732826241978	38.0	37.0	38.0	33.0	38.0
20	36.42686408469243	38.0	38.0	38.0	34.0	38.0
21	36.52337182524773	38.0	38.0	38.0	34.0	38.0
22	36.458099787604375	38.0	38.0	38.0	34.0	38.0
23	36.84114353701332	39.0	37.0	39.0	34.0	39.0
24	36.83594738416004	39.0	37.0	39.0	34.0	39.0
25	36.818438163736324	39.0	37.0	39.0	33.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	10.0
16	91.0
17	336.0
18	797.0
19	1285.0
20	2058.0
21	3061.0
22	4405.0
23	6198.0
24	8335.0
25	10705.0
26	13312.0
27	16354.0
28	20230.0
29	24301.0
30	29859.0
31	37009.0
32	47618.0
33	67224.0
34	111872.0
35	258689.0
36	800550.0
37	100702.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.958998187864054	19.993878609894928	13.158496719497204	24.888626482743813
2	13.927440441046796	21.70320331449632	41.66129926824468	22.7080569762122
3	17.67880465481238	26.79475579615132	30.159475630733212	25.36696391830308
4	11.441568200464664	18.03075263705396	40.06462603290471	30.463053129576668
5	12.768274074698851	38.13504630020115	35.503423633422024	13.593255991677978
6	28.972258217870365	38.83855887908273	19.691898551058014	12.497284351988878
7	26.39060347449591	32.41154655195769	23.76434820613877	17.433501767407623
8	24.444346468124042	36.31926819356372	22.160518439569486	17.07586689874275
9	26.24197765628714	16.198361154348486	21.39604755003821	36.163613639326165
10	13.978111238054344	29.79634556844583	34.52483188541371	21.700711308086113
11	33.153078202995005	23.63316643280145	25.027220377711494	18.18653498649205
12	22.14109356908992	26.020252983378956	32.219598160771476	19.61905528675965
13	27.31954678710086	22.572402370856558	27.005106696212916	23.10294414582966
14	21.02365233571288	22.77284914287759	28.362930701774562	27.840567819634966
15	22.456123087303986	30.671187211781703	24.348835114054094	22.523854586860217
16	21.957116823301785	29.13585721624848	27.421795327272402	21.485230633177338
17	21.100043961886065	28.861233795444324	28.254521428863534	21.784200813806077
18	21.301370957177955	28.350857686913127	29.791976178380985	20.555795177527934
19	22.954031361423148	27.818821965775918	27.86879065547164	21.358356017329292
20	22.835813456564193	27.748157160237604	28.35973203422928	21.05629734896893
21	23.589310394844208	27.445867308371835	26.88030711879959	22.08451517798437
22	22.255591054313097	28.407667731629395	27.780894568690094	21.555846645367414
23	21.600680384690836	27.975644681746992	27.76919061186699	22.654484321695183
24	21.751721088461455	28.226489746312776	28.410451642749702	21.611337522476067
25	22.252552748277935	27.776968395762246	27.828790144282994	22.141688711676828
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	92.0
1	92.0
2	171.5
3	251.0
4	251.0
5	251.0
6	645.5
7	1040.0
8	1040.0
9	1040.0
10	2160.0
11	3280.0
12	3280.0
13	3280.0
14	6259.5
15	9239.0
16	9239.0
17	9239.0
18	16366.0
19	23493.0
20	23493.0
21	23493.0
22	36537.0
23	49581.0
24	49581.0
25	49581.0
26	68541.5
27	87502.0
28	87502.0
29	87502.0
30	108708.5
31	129915.0
32	129915.0
33	129915.0
34	147551.5
35	165188.0
36	165188.0
37	165188.0
38	175796.5
39	186405.0
40	186405.0
41	186405.0
42	193633.0
43	200861.0
44	200861.0
45	200861.0
46	205006.0
47	209151.0
48	209151.0
49	209151.0
50	191616.0
51	174081.0
52	174081.0
53	174081.0
54	148332.0
55	122583.0
56	122583.0
57	122583.0
58	103442.0
59	84301.0
60	84301.0
61	84301.0
62	69615.5
63	54930.0
64	54930.0
65	54930.0
66	42789.5
67	30649.0
68	30649.0
69	30649.0
70	22501.5
71	14354.0
72	14354.0
73	14354.0
74	11104.0
75	7854.0
76	7854.0
77	7854.0
78	6938.0
79	6022.0
80	6022.0
81	6022.0
82	4251.5
83	2481.0
84	2481.0
85	2481.0
86	1837.0
87	1193.0
88	1193.0
89	1193.0
90	847.0
91	501.0
92	501.0
93	501.0
94	269.0
95	37.0
96	37.0
97	37.0
98	28.5
99	20.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	6.389760026172458E-5
16	5.111808020937966E-4
17	7.667712031406949E-4
18	0.0024920064102072585
19	0.0015335424062813898
20	0.0017891328073282881
21	4.4728320183207203E-4
22	2.555904010468983E-4
23	4.4728320183207203E-4
24	3.8338560157034744E-4
25	0.0015335424062813898
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1565004.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.8776366048949
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.00185731227894	75.72399614472911
2	2.3365378240764025	3.6860116280218067
3	0.5507751932389544	1.3033153662987904
4	0.2626557352505493	0.8287065454913725
5	0.16913818380780724	0.667061009920207
6	0.11099437380383663	0.5252984329252136
7	0.07401821784325849	0.4086867462427743
8	0.060357115709696284	0.3808661311575214
9	0.04659720781245799	0.33079298621704484
>10	0.30098269526241844	4.754111696056204
>50	0.04180333401276536	2.337497073822956
>100	0.04013911712047437	6.398782029282835
>500	0.0035749480476373607	1.9560679436237798
>1k	5.687417348513983E-4	0.6988062662103955
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	2219	0.14178877498076684	No Hit
TCCATGTACTCTGCGTTGATACCAC	1975	0.12619776051690604	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.389760026172456E-5
2	0.0	0.0	0.0	0.0	6.389760026172456E-5
3	0.0	0.0	0.0	0.0	6.389760026172456E-5
4	0.0	0.0	0.0	0.0	6.389760026172456E-5
5	0.0	0.0	0.0	0.0	6.389760026172456E-5
6	0.0	0.0	0.0	0.0	6.389760026172456E-5
7	0.0	0.0	0.0	0.0	6.389760026172456E-5
8	0.0	0.0	0.0	0.0	6.389760026172456E-5
9	0.0	0.0	0.0	0.0	6.389760026172456E-5
10	6.389760026172456E-5	0.0	0.0	0.0	6.389760026172456E-5
11	6.389760026172456E-5	0.0	0.0	0.0	6.389760026172456E-5
12	6.389760026172456E-5	0.0	0.0	0.0	1.2779520052344913E-4
13	6.389760026172456E-5	0.0	0.0	0.0	1.2779520052344913E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	120	0.0	17.416668	13
GCATCGC	125	0.0	16.72	14
ATCGCCA	135	0.0	15.481481	16
CATCGCC	150	0.0	14.566667	15
CCAACGA	100	6.6029315E-10	14.250001	19
TCGCCAG	150	0.0	13.933332	17
CGCCAGT	150	0.0	13.933332	18
AGGCCCG	165	0.0	13.242424	10
GCTCGTA	65	5.4525368E-5	13.153846	9
CGAGCCG	160	0.0	13.0625	15
CCGCATC	160	0.0	13.0625	12
AACCGCG	90	5.405036E-7	12.666666	7
TCCAACG	150	1.8189894E-12	12.666666	18
CGGTTCT	160	0.0	12.46875	12
CGAGGTT	55	0.0030684625	12.090909	4
TACACCG	55	0.0030684625	12.090909	5
GAGCCGC	180	0.0	11.611111	16
CGCGGTC	100	1.9294166E-6	11.400001	10
AAAGGCG	75	2.0738333E-4	11.4	5
GCCGCCT	185	0.0	11.2972975	18
>>END_MODULE