FastQCFastQC Report
Mon 15 Aug 2016
SRR2920844_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920844_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1565004
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29270.1870282759660678No Hit
GTACATGGAAGCAGTGGTATCAACG23130.14779514940536895No Hit
TCCATGTACTCTGCGTTGATACCAC20170.12888145972789847No Hit
TATCAACGCAGAGTACTTTTTTTTT17610.11252367406089697No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACCG456.7623664E-414.7777785
AACAGCG456.7623664E-414.7777787
CGCGAGG400.005279124714.257
GCATCGC1301.8189894E-1213.88461514
CGCGGTA551.960166E-413.81818217
CCAACGA852.6990529E-713.41176419
CGCATCG1351.8189894E-1213.3703713
CGCCCCT1001.0071744E-813.3000015
ATCGCCA1403.6379788E-1212.89285816
TCCGATA604.0920536E-412.6666678
CCGATAA604.0920536E-412.6666679
GCGTTAT604.0920536E-412.6666671
GCCGCGG604.0920536E-412.66666715
TCCCCGC1051.9934305E-812.6666671
CGATAAC604.0920536E-412.66666710
CGCCAGT1457.2759576E-1212.44827618
CGACCCG550.003068462512.0909095
CATCGCC1501.4551915E-1112.03333315
AGCCCCG951.039778E-612.016
GTCTTAC951.039778E-612.01
Produced by FastQC (version 0.11.5)