FastQCFastQC Report
Mon 15 Aug 2016
SRR2920843_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920843_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1096605
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAACCTCCG21160.19295917855563308No Hit
GAATAACGCCGCCGCATCGCCAGTC21070.19213846371300514No Hit
CTATTGGAGCTGGAATTACCGCGGC19960.18201631398726068No Hit
GAATAGGACCGCGGTTCTATTTTGT19910.18156036129691183No Hit
GAACTACGACGGTATCTGATCGTCT19610.17882464515481875No Hit
GTCCTATTCCATTATTCCTAGCTGC18750.17098225888081853No Hit
CTTTAATATACGCTATTGGAGCTGG18030.1644165401397951No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG17620.16067772807893455No Hit
GTCTTGCGCCGGTCCAAGAATTTCA16920.15429439041405063No Hit
CTCTTAATCATGGCCTCAGTTCCGA16770.1529265323430041No Hit
GTACATGGGTACCTGGTTGATCCTG16630.15164986481002732No Hit
ATACAGGACTCTTTCGAGGCCCTGT16400.1495524824344226No Hit
GATTAAGAGGGACGGCCGGGGGCAT16170.1474551000588179No Hit
GCTTTGAACACTCTAATTTTTTCAA15380.14025104755130607No Hit
GTCCTGTATTGTTATTTTTCGTCAC15070.1374241408711432No Hit
ATATTAAAGTTGCTGCAGTTAAAAA15070.1374241408711432No Hit
GTATCAACGCAGAGTACATGGGAAT14420.13149675589660817No Hit
CTCTAGATAACCTCGGGCCGATCGC14380.1311319937443291No Hit
GAATAATGGAATAGGACCGCGGTTC13050.11900365218104969No Hit
CCATTATTCCTAGCTGCGGTATCCA12740.11617674550088682No Hit
GTACAGTGAAACTGCGAATGGCTCA12630.11517364958211936No Hit
GTACATGGGAATAACGCCGCCGCAT12530.11426174420142166No Hit
GCTAAGAGCATCGAGGGGGCGCCGA11870.10824316868881685No Hit
CTTTAAATCCTTTAACGAGGATCCA11860.10815197815074706No Hit
ATCAGATACCGTCGTAGTTCCGACC11590.1056898336228633No Hit
GTATCAACGCAGAGTACATGGGTAC11480.10468673770409583No Hit
GATTTAAAGTGGACTCATTCCAATT11280.10286292694270043No Hit
AGATAACCTCGGGCCGATCGCACGC11180.10195102156200272No Hit
AGCTAATACATGCCGACGGGCGCTG11180.10195102156200272No Hit
CCAATGGATCCTCGTTAAAGGATTT11120.10140387833358412No Hit
GTATTGTTATTTTTCGTCACTACCT11110.10131268779551433No Hit
CTCCAATAGCGTATATTAAAGTTGC11050.10076554456709573No Hit
CTCCCGACCCGGGGAGGTAGTGACG11030.10058316349095618No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA602.5662037E-514.25041115
GCGTTAT1102.743036E-912.9543291
CGTCGTA2150.012.81373910
CGTTATT1051.9914296E-812.6664542
CGGTCCA3000.012.66645410
CGGTTCT4900.012.60240412
CGCATCG2950.012.55968513
CGCCAGT2950.012.55968518
CCGTCGT2050.012.5119849
ATCGCCA3000.012.35035616
TAGGGTC1001.4412217E-712.34979254
CGAACGA701.0900431E-412.21463716
CTTATAC701.09050714E-412.2140813
GCATCGC3050.012.14789114
CGACCAT2350.012.12745610
GGTTCTA5050.012.0399513
ACTGTTC3200.011.8748018
CATCGCC3150.011.76224315
ATAGGGT1052.7174428E-711.7617073
ACCGTCG2350.011.7232078
Produced by FastQC (version 0.11.5)