##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920843_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1096605
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.845452099890114	32.0	32.0	33.0	29.0	33.0
2	30.8190579105512	32.0	32.0	33.0	29.0	33.0
3	31.405592715699818	32.0	32.0	33.0	29.0	34.0
4	31.493610734950142	32.0	32.0	33.0	29.0	34.0
5	31.428027411875743	33.0	32.0	33.0	29.0	34.0
6	34.54684412345375	37.0	33.0	37.0	32.0	37.0
7	34.47656995910104	37.0	33.0	37.0	31.0	37.0
8	34.37112725183635	37.0	33.0	37.0	31.0	37.0
9	34.40874334879013	37.0	33.0	37.0	32.0	37.0
10	34.36687594895154	37.0	33.0	37.0	31.0	37.0
11	34.26656270945327	37.0	33.0	37.0	31.0	37.0
12	34.55215141276941	37.0	33.0	37.0	32.0	37.0
13	36.31487819223877	38.0	37.0	38.0	33.0	38.0
14	35.932327501698424	38.0	37.0	38.0	33.0	38.0
15	36.015894510785564	38.0	37.0	38.0	33.0	38.0
16	36.19226704237168	38.0	37.0	38.0	33.0	38.0
17	36.125507361356185	38.0	37.0	38.0	33.0	38.0
18	36.14959260627117	38.0	37.0	38.0	33.0	38.0
19	36.04688835086471	38.0	37.0	38.0	33.0	38.0
20	36.21127753384309	38.0	37.0	38.0	33.0	38.0
21	36.294741497622205	38.0	37.0	38.0	33.0	38.0
22	36.21510936025278	38.0	37.0	38.0	33.0	38.0
23	36.42249214621491	38.0	37.0	39.0	33.0	39.0
24	36.43232795765111	38.0	37.0	39.0	33.0	39.0
25	36.3618322002909	38.0	37.0	39.0	33.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	4.0
15	28.0
16	135.0
17	370.0
18	752.0
19	1133.0
20	1628.0
21	2278.0
22	3410.0
23	4360.0
24	5794.0
25	7419.0
26	9416.0
27	12019.0
28	15176.0
29	19324.0
30	24549.0
31	32314.0
32	43940.0
33	65304.0
34	114823.0
35	256040.0
36	456072.0
37	20316.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.42931137465176	18.62065192115666	12.559490427273264	25.390546276918307
2	16.185043839851176	20.50720177274406	38.23838118556819	25.069373201836576
3	18.319267192836072	25.056059383278388	29.14358406171776	27.481089362167783
4	12.673387409322409	16.016523725498242	37.05782847971694	34.2522603854624
5	14.788460749312652	36.443204253126694	32.860966346131924	15.907368651428728
6	32.61292808258215	36.27723747383971	17.637891492378753	13.471942951199384
7	29.29249821038569	30.80626114234387	21.55297486332818	18.348265783942256
8	27.244997059105145	32.93811354133895	20.38473288011636	19.43215651943954
9	27.23834014982605	15.378189959009852	19.659312149771342	37.72415774139276
10	16.1789340738005	26.7410781457316	30.720724417634425	26.359263362833474
11	36.38594494629774	21.885515437688426	22.6589045068311	19.06963510918273
12	24.80191135367794	23.69339917290182	28.80171073449419	22.70297873892605
13	28.558687950538253	19.65019309596436	25.843216107896644	25.94790284560074
14	23.77209660725603	21.199885099922035	25.100286794242226	29.927731498579707
15	24.734725515228888	27.44847711107058	22.637515958416927	25.179281415283604
16	25.583030426829158	26.41724679417617	23.903563566594137	24.096159212400533
17	23.57930944549365	26.447090407870892	25.59892102568218	24.374679120953278
18	24.547230307938108	25.395227360603474	26.675658225908823	23.381884105549595
19	26.19018958772194	25.03624873471398	25.140024986549214	23.63353669101487
20	25.429149324997173	25.009392771031603	24.763083309319452	24.798374594651772
21	27.23655749245848	24.75287161361158	23.917194664215444	24.093376229714497
22	25.205681571551285	24.727384146241377	25.128625075004695	24.93830920720264
23	23.947265950937307	24.16037979312364	25.43286184441504	26.459492411524018
24	24.520813898243567	25.74614786261888	25.088660485738178	24.644377753399375
25	24.866130703123858	24.844062331295504	25.166971854618676	25.12283511096196
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	32.0
1	32.0
2	64.5
3	97.0
4	97.0
5	97.0
6	239.0
7	381.0
8	381.0
9	381.0
10	778.0
11	1175.0
12	1175.0
13	1175.0
14	2154.0
15	3133.0
16	3133.0
17	3133.0
18	5543.0
19	7953.0
20	7953.0
21	7953.0
22	13658.5
23	19364.0
24	19364.0
25	19364.0
26	29166.0
27	38968.0
28	38968.0
29	38968.0
30	49224.0
31	59480.0
32	59480.0
33	59480.0
34	72417.5
35	85355.0
36	85355.0
37	85355.0
38	91110.0
39	96865.0
40	96865.0
41	96865.0
42	105865.0
43	114865.0
44	114865.0
45	114865.0
46	127754.0
47	140643.0
48	140643.0
49	140643.0
50	138186.5
51	135730.0
52	135730.0
53	135730.0
54	123780.0
55	111830.0
56	111830.0
57	111830.0
58	102783.5
59	93737.0
60	93737.0
61	93737.0
62	84358.5
63	74980.0
64	74980.0
65	74980.0
66	61914.0
67	48848.0
68	48848.0
69	48848.0
70	37028.0
71	25208.0
72	25208.0
73	25208.0
74	20419.0
75	15630.0
76	15630.0
77	15630.0
78	14188.5
79	12747.0
80	12747.0
81	12747.0
82	9104.0
83	5461.0
84	5461.0
85	5461.0
86	4149.0
87	2837.0
88	2837.0
89	2837.0
90	2003.0
91	1169.0
92	1169.0
93	1169.0
94	629.0
95	89.0
96	89.0
97	89.0
98	58.5
99	28.0
100	28.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	2.735716142093096E-4
12	0.0
13	0.0
14	0.0
15	4.559526903488494E-4
16	6.383337664883892E-4
17	7.295243045581591E-4
18	0.0019150012994651675
19	0.0013678580710465482
20	0.001550239147186088
21	8.20714842627929E-4
22	6.383337664883892E-4
23	7.295243045581591E-4
24	5.471432284186192E-4
25	0.0011854769949070083
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1096605.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.539328505564626
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.3127140911308	52.540722528242746
2	5.017207378642404	5.77373487080496
3	1.3889405021148624	2.3975611147761287
4	0.6400174459633476	1.4730469629031007
5	0.3411863741494404	0.9815817431903567
6	0.20770921948437449	0.717086940812751
7	0.15604872053521643	0.6285257015624207
8	0.11145590384692791	0.5130478292266408
9	0.08308676366512968	0.43026809300928964
>10	0.5197640168254372	5.943958327078492
>50	0.08818058899061854	3.6099545168399914
>100	0.11029342096753758	13.234547922158246
>500	0.01702943118494942	6.603079630755576
>1k	0.006366142499046512	5.152883818639283
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCTGATCGTCTTCGAACCTCCG	2116	0.19295917855563308	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2107	0.19213846371300514	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1996	0.18201631398726068	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1991	0.18156036129691183	No Hit
GAACTACGACGGTATCTGATCGTCT	1961	0.17882464515481875	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1875	0.17098225888081853	No Hit
CTTTAATATACGCTATTGGAGCTGG	1803	0.1644165401397951	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1762	0.16067772807893455	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1692	0.15429439041405063	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	1677	0.1529265323430041	No Hit
GTACATGGGTACCTGGTTGATCCTG	1663	0.15164986481002732	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	1640	0.1495524824344226	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1617	0.1474551000588179	No Hit
GCTTTGAACACTCTAATTTTTTCAA	1538	0.14025104755130607	No Hit
GTCCTGTATTGTTATTTTTCGTCAC	1507	0.1374241408711432	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	1507	0.1374241408711432	No Hit
GTATCAACGCAGAGTACATGGGAAT	1442	0.13149675589660817	No Hit
CTCTAGATAACCTCGGGCCGATCGC	1438	0.1311319937443291	No Hit
GAATAATGGAATAGGACCGCGGTTC	1305	0.11900365218104969	No Hit
CCATTATTCCTAGCTGCGGTATCCA	1274	0.11617674550088682	No Hit
GTACAGTGAAACTGCGAATGGCTCA	1263	0.11517364958211936	No Hit
GTACATGGGAATAACGCCGCCGCAT	1253	0.11426174420142166	No Hit
GCTAAGAGCATCGAGGGGGCGCCGA	1187	0.10824316868881685	No Hit
CTTTAAATCCTTTAACGAGGATCCA	1186	0.10815197815074706	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1159	0.1056898336228633	No Hit
GTATCAACGCAGAGTACATGGGTAC	1148	0.10468673770409583	No Hit
GATTTAAAGTGGACTCATTCCAATT	1128	0.10286292694270043	No Hit
AGATAACCTCGGGCCGATCGCACGC	1118	0.10195102156200272	No Hit
AGCTAATACATGCCGACGGGCGCTG	1118	0.10195102156200272	No Hit
CCAATGGATCCTCGTTAAAGGATTT	1112	0.10140387833358412	No Hit
GTATTGTTATTTTTCGTCACTACCT	1111	0.10131268779551433	No Hit
CTCCAATAGCGTATATTAAAGTTGC	1105	0.10076554456709573	No Hit
CTCCCGACCCGGGGAGGTAGTGACG	1103	0.10058316349095618	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	9.119053806976988E-5
7	0.0	0.0	0.0	0.0	9.119053806976988E-5
8	0.0	0.0	0.0	0.0	9.119053806976988E-5
9	0.0	0.0	0.0	0.0	9.119053806976988E-5
10	0.0	0.0	0.0	0.0	9.119053806976988E-5
11	0.0	0.0	0.0	0.0	9.119053806976988E-5
12	0.0	0.0	0.0	0.0	1.8238107613953976E-4
13	0.0	0.0	0.0	0.0	2.735716142093096E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTA	60	2.5662037E-5	14.250411	15
GCGTTAT	110	2.743036E-9	12.954329	1
CGTCGTA	215	0.0	12.813739	10
CGTTATT	105	1.9914296E-8	12.666454	2
CGGTCCA	300	0.0	12.666454	10
CGGTTCT	490	0.0	12.602404	12
CGCATCG	295	0.0	12.559685	13
CGCCAGT	295	0.0	12.559685	18
CCGTCGT	205	0.0	12.511984	9
ATCGCCA	300	0.0	12.350356	16
TAGGGTC	100	1.4412217E-7	12.3497925	4
CGAACGA	70	1.0900431E-4	12.214637	16
CTTATAC	70	1.09050714E-4	12.214081	3
GCATCGC	305	0.0	12.147891	14
CGACCAT	235	0.0	12.127456	10
GGTTCTA	505	0.0	12.03995	13
ACTGTTC	320	0.0	11.874801	8
CATCGCC	315	0.0	11.762243	15
ATAGGGT	105	2.7174428E-7	11.761707	3
ACCGTCG	235	0.0	11.723207	8
>>END_MODULE