FastQCFastQC Report
Mon 15 Aug 2016
SRR2920843_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920843_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1096605
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAACCTCCG21020.1916825110226563No Hit
CTATTGGAGCTGGAATTACCGCGGC20710.18885560434249343No Hit
GAATAGGACCGCGGTTCTATTTTGT20180.18402250582479562No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG19970.18210750452533045No Hit
GAACTACGACGGTATCTGATCGTCT19160.17472107094167907No Hit
GTCCTATTCCATTATTCCTAGCTGC18710.17061749672853946No Hit
CTTTAATATACGCTATTGGAGCTGG18280.16669630359153934No Hit
ATACAGGACTCTTTCGAGGCCCTGT17270.1574860592464926No Hit
GATTAAGAGGGACGGCCGGGGGCAT17260.1573948687084228No Hit
GAATAACGCCGCCGCATCGCCAGTC16600.151376293195818No Hit
ATATTAAAGTTGCTGCAGTTAAAAA16430.14982605404863192No Hit
CTCTTAATCATGGCCTCAGTTCCGA15970.1456312892974225No Hit
GTATCAACGCAGAGTACTTTTTTTT15930.1452665271451434No Hit
GTCTTGCGCCGGTCCAAGAATTTCA15860.144628193378655No Hit
GTACATGGGTACCTGGTTGATCCTG15350.13997747593709678No Hit
GCTTTGAACACTCTAATTTTTTCAA14790.13487080580518965No Hit
CTCTAGATAACCTCGGGCCGATCGC13950.12721080060732898No Hit
GAATAATGGAATAGGACCGCGGTTC13570.12374556016067774No Hit
GCGCAAGACGGACCAGAGCGAAAGC13530.12338079800839866No Hit
GTCCTGTATTGTTATTTTTCGTCAC13440.12256008316577072No Hit
GCTAAGAGCATCGAGGGGGCGCCGA13180.12018912917595671No Hit
AGATAACCTCGGGCCGATCGCACGC12590.11480888742984027No Hit
ATCAGATACCGTCGTAGTTCCGACC12560.11453531581563096No Hit
ACCTCGGGCCGATCGCACGCCCCCC12200.11125245644511925No Hit
CCATTATTCCTAGCTGCGGTATCCA12150.11079650375477042No Hit
GTATCAACGCAGAGTACATGGGAAT12090.11024936052635179No Hit
CTTTAAATCCTTTAACGAGGATCCA12040.10979340783600294No Hit
GTACAGTGAAACTGCGAATGGCTCA11930.10879031191723545No Hit
GTATCAACGCAGAGTACATGGGTAC11890.10842554976495639No Hit
CCAATGGATCCTCGTTAAAGGATTT11880.10833435922688661No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG11240.10249816479042134No Hit
AAATAGAACCGCGGTCCTATTCCAT11070.10094792564323525No Hit
TATCTAGAGTCACCAAAGCCGCCGG10980.10012721080060734No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATACG402.7601878E-416.6251
CGTTATT902.2846507E-914.7777782
GCGTTAT851.7147613E-814.5294121
CGCATCG2550.013.78431313
GCATCGC2700.013.3703714
CGGTCCA3400.013.13235310
GTATTAT801.9978725E-613.06251
CCCTTAT604.0903193E-412.6666671
CATCGCC2850.012.66666715
CCGCATC2850.012.66666712
TCGCGTA751.4776433E-512.6666679
ATCGCCA2850.012.66666716
GTATCAA15900.012.6666661
CGCCAGT2900.012.448276518
CGGAATG701.0903364E-412.21428516
GTATTGA550.003067455712.0909091
TCGCCAG2950.011.91525417
CGCGTAA802.864559E-511.87510
GCGTAAC802.864559E-511.87511
AACACCG658.0201175E-411.69230711
Produced by FastQC (version 0.11.5)