##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920843_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1096605
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13780987684718	33.0	32.0	33.0	32.0	34.0
2	32.183561081702166	33.0	32.0	33.0	32.0	34.0
3	32.386430847935216	33.0	32.0	34.0	32.0	34.0
4	32.42362108507621	33.0	32.0	34.0	32.0	34.0
5	32.191476420406616	33.0	32.0	34.0	31.0	34.0
6	35.17670720086084	37.0	34.0	37.0	32.0	37.0
7	35.10039075145563	37.0	34.0	37.0	32.0	37.0
8	34.87641037565942	37.0	34.0	37.0	32.0	37.0
9	34.94469476247144	37.0	34.0	37.0	32.0	37.0
10	34.843644703425575	37.0	34.0	37.0	32.0	37.0
11	34.86957108530419	37.0	33.0	37.0	32.0	37.0
12	34.76080995435913	37.0	33.0	37.0	32.0	37.0
13	36.61612978237378	38.0	37.0	38.0	34.0	38.0
14	36.55745596636893	38.0	37.0	38.0	34.0	38.0
15	36.4934237943471	38.0	37.0	38.0	34.0	38.0
16	36.62243834379745	38.0	37.0	38.0	34.0	38.0
17	36.59070312464379	38.0	37.0	38.0	34.0	38.0
18	36.64271000041036	38.0	37.0	38.0	34.0	38.0
19	36.54378650471227	38.0	37.0	38.0	34.0	38.0
20	36.62427856885569	38.0	37.0	38.0	34.0	38.0
21	36.749135741675445	38.0	38.0	38.0	34.0	38.0
22	36.69303441074954	38.0	37.0	38.0	34.0	38.0
23	37.063826993311174	38.0	37.0	39.0	34.0	39.0
24	37.05951003323895	38.0	37.0	39.0	34.0	39.0
25	36.88667295881379	38.0	37.0	39.0	34.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	4.0
16	4.0
17	10.0
18	7.0
19	7.0
20	13.0
21	26.0
22	113.0
23	312.0
24	783.0
25	1719.0
26	3366.0
27	6075.0
28	9954.0
29	14863.0
30	21690.0
31	30146.0
32	41573.0
33	60872.0
34	100111.0
35	219124.0
36	525648.0
37	60185.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.81441357644731	19.085450093698277	12.805157736833225	25.294978593021188
2	16.486610949247908	20.84761605135851	37.82556161972634	24.840211379667245
3	18.444289420529724	25.297714309163283	29.047651615668357	27.210344654638636
4	12.991368815571697	16.71130443505182	36.59002101941903	33.70730572995746
5	14.798309327424185	36.405724941980026	32.88285207526867	15.913113655327123
6	32.39480031551926	36.762644707985096	17.331673665540464	13.510881310955176
7	29.114950232763846	31.116856115009504	21.37232640741197	18.395867244814678
8	27.274086840749405	33.040520515591304	20.21794538598675	19.467447257672543
9	27.15335056834503	15.292835615376548	19.754971024206526	37.798842792071895
10	16.221702436155226	27.14541699153296	30.3838665700047	26.24901400230712
11	36.347089426001155	21.987315396154493	22.577044605851697	19.08855057199265
12	24.75813989540445	24.0249679693235	28.652158252059767	22.56473388321228
13	28.626351329786022	19.773573893972763	25.807560607511366	25.792514168729852
14	23.57877266654812	21.07313025200505	25.226585689468862	30.12151139197797
15	24.761422754774966	27.6599140073226	22.593458902704256	24.98520433519818
16	25.429234254115435	26.53747387388702	23.87343106536179	24.15986080663576
17	23.414903269636742	26.631284737895594	25.51337993169829	24.440432060769375
18	24.364287961481118	25.34513338895956	26.846494407740252	23.44408424181907
19	26.0152926532343	24.98438362035555	25.412249624978912	23.588074101431236
20	25.420274392329052	25.03198508122797	24.944715736295205	24.603024790147774
21	27.207700129034613	24.809480168337732	24.059893945404227	23.92292575722343
22	25.04739628216176	24.749203222673614	25.350787202319886	24.852613292844737
23	23.74847825789596	24.044482744470432	25.90221638602779	26.30482261160582
24	24.51995020996621	25.688101002639968	25.254581184656278	24.53736760273754
25	24.724764158470915	24.79096848910957	25.50116039959694	24.983106952822574
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	46.0
1	46.0
2	96.0
3	146.0
4	146.0
5	146.0
6	526.5
7	907.0
8	907.0
9	907.0
10	1175.5
11	1444.0
12	1444.0
13	1444.0
14	2351.5
15	3259.0
16	3259.0
17	3259.0
18	5754.5
19	8250.0
20	8250.0
21	8250.0
22	14275.0
23	20300.0
24	20300.0
25	20300.0
26	30284.5
27	40269.0
28	40269.0
29	40269.0
30	50387.0
31	60505.0
32	60505.0
33	60505.0
34	72923.5
35	85342.0
36	85342.0
37	85342.0
38	90723.5
39	96105.0
40	96105.0
41	96105.0
42	105711.0
43	115317.0
44	115317.0
45	115317.0
46	127426.5
47	139536.0
48	139536.0
49	139536.0
50	137374.5
51	135213.0
52	135213.0
53	135213.0
54	123575.0
55	111937.0
56	111937.0
57	111937.0
58	102111.0
59	92285.0
60	92285.0
61	92285.0
62	83695.5
63	75106.0
64	75106.0
65	75106.0
66	61961.5
67	48817.0
68	48817.0
69	48817.0
70	36636.0
71	24455.0
72	24455.0
73	24455.0
74	19846.5
75	15238.0
76	15238.0
77	15238.0
78	14026.0
79	12814.0
80	12814.0
81	12814.0
82	9114.5
83	5415.0
84	5415.0
85	5415.0
86	4057.0
87	2699.0
88	2699.0
89	2699.0
90	1920.5
91	1142.0
92	1142.0
93	1142.0
94	599.5
95	57.0
96	57.0
97	57.0
98	29.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	9.119053806976989E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1096605.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.14495877757049
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.08854748513072	51.14162743661436
2	5.131436465667638	5.76208577669263
3	1.413892036336395	2.3814873028812626
4	0.6402541900126169	1.437881804217006
5	0.36727570503879314	1.0310339659703087
6	0.2270952744109342	0.7650152892229776
7	0.15544914696409687	0.61093801638154
8	0.11667350420818227	0.5240503267362472
9	0.08477571600128087	0.4283756172207842
>10	0.5415925033882798	6.017985969440458
>50	0.09356587303253248	3.7813590397152783
>100	0.11422211701081599	13.730206191967595
>500	0.018711600140267497	7.208408451891682
>1k	0.006508382657484346	5.179544811047874
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCTGATCGTCTTCGAACCTCCG	2102	0.1916825110226563	No Hit
CTATTGGAGCTGGAATTACCGCGGC	2071	0.18885560434249343	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2018	0.18402250582479562	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1997	0.18210750452533045	No Hit
GAACTACGACGGTATCTGATCGTCT	1916	0.17472107094167907	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1871	0.17061749672853946	No Hit
CTTTAATATACGCTATTGGAGCTGG	1828	0.16669630359153934	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	1727	0.1574860592464926	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1726	0.1573948687084228	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1660	0.151376293195818	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	1643	0.14982605404863192	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	1597	0.1456312892974225	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1593	0.1452665271451434	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1586	0.144628193378655	No Hit
GTACATGGGTACCTGGTTGATCCTG	1535	0.13997747593709678	No Hit
GCTTTGAACACTCTAATTTTTTCAA	1479	0.13487080580518965	No Hit
CTCTAGATAACCTCGGGCCGATCGC	1395	0.12721080060732898	No Hit
GAATAATGGAATAGGACCGCGGTTC	1357	0.12374556016067774	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1353	0.12338079800839866	No Hit
GTCCTGTATTGTTATTTTTCGTCAC	1344	0.12256008316577072	No Hit
GCTAAGAGCATCGAGGGGGCGCCGA	1318	0.12018912917595671	No Hit
AGATAACCTCGGGCCGATCGCACGC	1259	0.11480888742984027	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1256	0.11453531581563096	No Hit
ACCTCGGGCCGATCGCACGCCCCCC	1220	0.11125245644511925	No Hit
CCATTATTCCTAGCTGCGGTATCCA	1215	0.11079650375477042	No Hit
GTATCAACGCAGAGTACATGGGAAT	1209	0.11024936052635179	No Hit
CTTTAAATCCTTTAACGAGGATCCA	1204	0.10979340783600294	No Hit
GTACAGTGAAACTGCGAATGGCTCA	1193	0.10879031191723545	No Hit
GTATCAACGCAGAGTACATGGGTAC	1189	0.10842554976495639	No Hit
CCAATGGATCCTCGTTAAAGGATTT	1188	0.10833435922688661	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	1124	0.10249816479042134	No Hit
AAATAGAACCGCGGTCCTATTCCAT	1107	0.10094792564323525	No Hit
TATCTAGAGTCACCAAAGCCGCCGG	1098	0.10012721080060734	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	9.119053806976988E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATACG	40	2.7601878E-4	16.625	1
CGTTATT	90	2.2846507E-9	14.777778	2
GCGTTAT	85	1.7147613E-8	14.529412	1
CGCATCG	255	0.0	13.784313	13
GCATCGC	270	0.0	13.37037	14
CGGTCCA	340	0.0	13.132353	10
GTATTAT	80	1.9978725E-6	13.0625	1
CCCTTAT	60	4.0903193E-4	12.666667	1
CATCGCC	285	0.0	12.666667	15
CCGCATC	285	0.0	12.666667	12
TCGCGTA	75	1.4776433E-5	12.666667	9
ATCGCCA	285	0.0	12.666667	16
GTATCAA	1590	0.0	12.666666	1
CGCCAGT	290	0.0	12.4482765	18
CGGAATG	70	1.0903364E-4	12.214285	16
GTATTGA	55	0.0030674557	12.090909	1
TCGCCAG	295	0.0	11.915254	17
CGCGTAA	80	2.864559E-5	11.875	10
GCGTAAC	80	2.864559E-5	11.875	11
AACACCG	65	8.0201175E-4	11.692307	11
>>END_MODULE