FastQCFastQC Report
Mon 15 Aug 2016
SRR2920842_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920842_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813997
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG500.001498477413.2999994
GTTCTAC1251.4060788E-912.161
GGGTATA550.003066287812.0909091
ATAAGAC550.003066287812.0909093
GTGTATA1353.6925485E-1011.9629631
GTAGGAC1052.713532E-711.7619043
GTTAGAC658.016223E-411.6923073
TAACACC752.0715022E-411.4000014
GTGCTAA600.00587158611.0833331
GCCTAAG1002.3945231E-510.451
TAGGCTG1002.3945231E-510.455
GTCCTAC1106.032622E-610.3636361
GTGTTAG750.00264525510.1333341
TAATACC750.00264525510.1333344
TATAGAC750.00264525510.1333342
TATCCTC750.00264525510.1333345
CTCTTAG856.58576E-410.0588241
GTGCTAG1701.551598E-910.0588241
AATTATA856.58576E-410.0588242
TTGGACA951.6416403E-410.04
Produced by FastQC (version 0.11.5)