##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920842_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	813997
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.799429236225688	33.0	32.0	33.0	30.0	34.0
2	31.933734399512527	33.0	32.0	34.0	30.0	34.0
3	32.259250341217474	33.0	32.0	34.0	32.0	34.0
4	32.32160683638883	33.0	32.0	34.0	32.0	34.0
5	32.379876092909434	33.0	32.0	34.0	32.0	34.0
6	35.251267510813925	37.0	34.0	37.0	33.0	37.0
7	35.2492306482702	37.0	35.0	37.0	33.0	37.0
8	35.233292014589736	37.0	34.0	37.0	33.0	37.0
9	35.25961275041554	37.0	34.0	37.0	33.0	37.0
10	35.26402062906866	37.0	34.0	37.0	33.0	37.0
11	35.204361932537836	37.0	34.0	37.0	33.0	37.0
12	35.11857660409068	37.0	34.0	37.0	33.0	37.0
13	36.871892648253	38.0	38.0	38.0	36.0	38.0
14	36.911954220961505	38.0	38.0	38.0	36.0	38.0
15	36.87040861329956	38.0	38.0	38.0	36.0	38.0
16	36.83991955744309	38.0	38.0	38.0	36.0	38.0
17	36.83925125031173	38.0	38.0	38.0	36.0	38.0
18	36.8212659260415	38.0	38.0	38.0	36.0	38.0
19	36.87029067674697	38.0	38.0	38.0	36.0	38.0
20	36.88126000464375	38.0	38.0	38.0	36.0	38.0
21	36.919516902396445	38.0	38.0	38.0	36.0	38.0
22	36.776950037899404	38.0	38.0	38.0	36.0	38.0
23	37.37410334436122	39.0	38.0	39.0	36.0	39.0
24	37.4187251304366	39.0	38.0	39.0	36.0	39.0
25	37.21552659284985	39.0	38.0	39.0	35.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	42.0
19	162.0
20	455.0
21	997.0
22	1671.0
23	2596.0
24	3544.0
25	4377.0
26	5337.0
27	6451.0
28	7650.0
29	9021.0
30	10867.0
31	13491.0
32	17510.0
33	25030.0
34	42860.0
35	116135.0
36	475818.0
37	69981.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.054711503850754	17.07745851643188	12.011100778012695	25.856729201704674
2	16.610503478514048	19.058792599972726	38.19424395913007	26.136459962383153
3	20.423048242192536	21.803151608666862	27.841380250787168	29.932419898353434
4	13.829657848861851	15.104232570881711	35.69005782576594	35.3760517544905
5	15.383226228106492	35.27887694917794	34.396932666828015	14.940964155887551
6	34.964748027326884	34.22260770002838	16.789005364884638	14.023638907760102
7	30.34962045314663	29.499740171032574	20.5915992319382	19.559040143882594
8	26.544815275731974	34.45737515003127	19.57513356928834	19.42267600494842
9	25.37331218665425	16.476596351092205	18.99429604777413	39.155795414479414
10	15.242808020176978	27.297520752533487	32.814617252889136	24.645053974400398
11	35.95873203463895	22.071948668115486	23.085711618101787	18.88360767914378
12	24.1093026141374	23.14492559554888	29.534875435658854	23.21089635465487
13	28.59838549773525	20.432507736515	25.594566073339337	25.374540692410413
14	22.42489837186132	19.880048697968174	26.023683133967324	31.67136979620318
15	24.079204223111386	28.018407930250355	22.410033452211742	25.492354394426513
16	24.78682353866169	26.971966727150097	24.0740444989355	24.16716523525271
17	23.541978655940994	26.857347140100025	26.038179501890056	23.562494702068925
18	23.68018555350941	26.626019506214398	26.245059871228026	23.448735069048166
19	24.624660778848078	26.262750354116783	25.652674395605878	23.459914471429254
20	24.19075254577105	26.202799273215994	26.016803501732806	23.589644679280145
21	24.107705556654384	25.83523035097181	26.00058722575145	24.056476866622358
22	24.09406914276097	25.643460602434654	25.895549983599448	24.366920271204933
23	23.88338040557889	25.65906262553793	26.018277708640202	24.439279260242973
24	23.91016183106326	25.920980052752036	25.97810557041365	24.19075254577105
25	24.258074661208827	25.651691591000947	25.89112736287726	24.199106384912966
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.0
2	10.5
3	19.0
4	19.0
5	19.0
6	61.5
7	104.0
8	104.0
9	104.0
10	264.5
11	425.0
12	425.0
13	425.0
14	933.5
15	1442.0
16	1442.0
17	1442.0
18	2888.0
19	4334.0
20	4334.0
21	4334.0
22	7245.5
23	10157.0
24	10157.0
25	10157.0
26	15883.0
27	21609.0
28	21609.0
29	21609.0
30	29413.5
31	37218.0
32	37218.0
33	37218.0
34	46709.5
35	56201.0
36	56201.0
37	56201.0
38	66956.5
39	77712.0
40	77712.0
41	77712.0
42	87303.0
43	96894.0
44	96894.0
45	96894.0
46	103719.0
47	110544.0
48	110544.0
49	110544.0
50	111223.0
51	111902.0
52	111902.0
53	111902.0
54	106460.5
55	101019.0
56	101019.0
57	101019.0
58	90155.0
59	79291.0
60	79291.0
61	79291.0
62	66370.0
63	53449.0
64	53449.0
65	53449.0
66	41845.5
67	30242.0
68	30242.0
69	30242.0
70	22122.0
71	14002.0
72	14002.0
73	14002.0
74	9570.0
75	5138.0
76	5138.0
77	5138.0
78	3423.0
79	1708.0
80	1708.0
81	1708.0
82	1079.0
83	450.0
84	450.0
85	450.0
86	278.5
87	107.0
88	107.0
89	107.0
90	66.5
91	26.0
92	26.0
93	26.0
94	13.5
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	813997.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.88597972074989
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.37834425994977	84.83289938227794
2	3.849545651242195	6.920403646833137
3	0.8761412668781023	2.3625844844135164
4	0.342493209655853	1.2314135079048216
5	0.17403170611539454	0.7821505203327929
6	0.1065129308476238	0.5744411485300288
7	0.0641680561564575	0.4037466016079495
8	0.04662469641028704	0.33527252128169444
9	0.03322341317537137	0.2687687138641973
>10	0.12291935998179021	1.8694336608647124
>50	0.004861224715251756	0.2956708508122577
>100	0.0011342248719371916	0.12321496127694469
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.2285057561637206E-4	0.0	0.0	0.0
5	0.0	1.2285057561637206E-4	0.0	0.0	0.0
6	0.0	1.2285057561637206E-4	0.0	0.0	0.0
7	0.0	1.2285057561637206E-4	0.0	0.0	0.0
8	0.0	1.2285057561637206E-4	0.0	0.0	0.0
9	0.0	1.2285057561637206E-4	0.0	0.0	0.0
10	0.0	1.2285057561637206E-4	0.0	0.0	0.0
11	0.0	1.2285057561637206E-4	0.0	0.0	0.0
12	0.0	1.2285057561637206E-4	0.0	0.0	3.6855172684911616E-4
13	0.0	1.2285057561637206E-4	0.0	0.0	3.6855172684911616E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	50	0.0014984774	13.299999	4
GTTCTAC	125	1.4060788E-9	12.16	1
GGGTATA	55	0.0030662878	12.090909	1
ATAAGAC	55	0.0030662878	12.090909	3
GTGTATA	135	3.6925485E-10	11.962963	1
GTAGGAC	105	2.713532E-7	11.761904	3
GTTAGAC	65	8.016223E-4	11.692307	3
TAACACC	75	2.0715022E-4	11.400001	4
GTGCTAA	60	0.005871586	11.083333	1
GCCTAAG	100	2.3945231E-5	10.45	1
TAGGCTG	100	2.3945231E-5	10.45	5
GTCCTAC	110	6.032622E-6	10.363636	1
GTGTTAG	75	0.002645255	10.133334	1
TAATACC	75	0.002645255	10.133334	4
TATAGAC	75	0.002645255	10.133334	2
TATCCTC	75	0.002645255	10.133334	5
CTCTTAG	85	6.58576E-4	10.058824	1
GTGCTAG	170	1.551598E-9	10.058824	1
AATTATA	85	6.58576E-4	10.058824	2
TTGGACA	95	1.6416403E-4	10.0	4
>>END_MODULE